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1. 发明申请

WO2006046152A3 METHODS FOR HETEROLOGOUS EXPRESSION OF SECONDARY METABOLITES 审中-公开
公开(公告)号：WO2006046152A3
公开(公告)日：2006-05-04
申请号：PCT/IB2005/003650
申请日：2005-10-26
申请人： GENE BRIDGES GMBH , ZHANG, Youming , MÜLLER, Rolf , STEWART, Francis , GROSS, Frank , WENZEL, Silke, C. , FU, Jun
发明人： ZHANG, Youming , MÜLLER, Rolf , STEWART, Francis , GROSS, Frank , WENZEL, Silke, C. , FU, Jun
IPC分类号： C12N15/63 , C12N1/21
摘要： The invention provides a method for the heterologous expression of a secondary metabolite encoded by a biosynthetic pathway. Also provided is a method for introducing a large sized DNA molecule into the chromosome of a heterologous host using a transposable element. Novel myxochromide S derivatives are also provided.

2. 发明申请

WO2011154927A9 DIRECT CLONING 审中-公开
公开(公告)号：WO2011154927A9
公开(公告)日：2011-12-15
申请号：PCT/IB2011/052549
申请日：2011-06-10
申请人： GENE BRIDGES GMBH , ZHANG, Youming , FU, Jun , STEWART, Francis
发明人： ZHANG, Youming , FU, Jun , STEWART, Francis
IPC分类号： C12N15/90 , C12N9/22
摘要： A method for performing homologous recombination between at least a first nucleic acid molecule and a second nucleic acid molecule which share at least one region of sequence homology. A method for improving the efficiency of homologous recombination.

3. 发明申请

WO2009104094A2 METHOD OF NUCLEIC ACID RECOMBINATION 审中-公开
标题翻译：核酸重组方法
公开(公告)号：WO2009104094A2
公开(公告)日：2009-08-27
申请号：PCT/IB2009/000488
申请日：2009-02-20
申请人： GENE BRIDGES GMBH , MARESCA, Marcello , ERLER, Axel, Steffen , FU, Jun , SEIBERT, Philipp, Martin , STEWART, Adrian, Francis , ZHANG, Youming
发明人： MARESCA, Marcello , ERLER, Axel, Steffen , FU, Jun , SEIBERT, Philipp, Martin , STEWART, Adrian, Francis , ZHANG, Youming
IPC分类号： C12N15/09
CPC分类号： C12N15/1082
摘要： The invention provides a method for inserting a single stranded replacement nucleic acid into a target nucleic acid, said method comprising the steps of: a) generating a single stranded replacement nucleic acid from a double stranded nucleic acid, wherein the double stranded nucleic acid is adapted at one or both of its 5' ends such that preferential degradation of one strand and/or strand separation generates the single stranded replacement nucleic acid, wherein the single stranded replacement nucleic acid comprises a 5' region that is identical to sequence on the target nucleic acid, a 3' region that is identical to sequence on the target nucleic acid and optionally a replacement region between the 5' and 3' regions that is not identical to sequence on the target nucleic acid, b) exposing the target nucleic acid to the single stranded replacement nucleic acid under conditions suitable for recombination to occur between the single stranded replacement nucleic acid and the target nucleic acid, and c) selecting a target nucleic acid whose sequence has been altered by inclusion of said single stranded replacement nucleic acid. Other methods for modifying target nucleic acids are also provided.
摘要翻译：本发明提供了将单链置换核酸插入靶核酸的方法，所述方法包括以下步骤：a）从双链核酸产生单链置换核酸，其中双链核酸适应在其5'末端的一个或两个上，使得一条链和/或链分离的优先降解产生单链置换核酸，其中单链置换核酸包含与靶核酸上的序列相同的5'区酸，3'区，其与靶核酸上的序列相同，并且任选地在与靶核酸上的序列不相同的5'和3'区之间的置换区，b）将靶核酸暴露于单链置换核酸在适于在单链置换核酸和靶核之间发生重组的条件下进行并且c）选择通过包含所述单链置换核酸改变其序列的靶核酸。还提供了用于修饰靶核酸的其它方法。

4. 发明申请

WO2010049807A2 METHOD FOR RECOMBINANT EXPRESSION OF POLYPEPTIDES 审中-公开
标题翻译：多糖重组表达方法
公开(公告)号：WO2010049807A2
公开(公告)日：2010-05-06
申请号：PCT/IB2009/007384
申请日：2009-10-29
申请人： GENE BRIDGES GMBH , ZHANG, Youming
发明人： ZHANG, Youming
IPC分类号： C12N15/73
CPC分类号： C12N15/73
摘要： A two vector system for broad host range inducible expression of a polypeptide of interest comprising: i). a first vector comprising: (a) the T7 promoter positioned 5' of an inducible promoter; (b) a cloning site positioned 3' of the inducible promoter for insertion of a gene encoding the polypeptide of interest; and ii). a second vector for making a host cell competent for T7 RNA polymerase expression, wherein the vector comprises a gene encoding the T7 RNA polymerase under the control of an inducible promoter.
摘要翻译：用于广泛宿主范围的两种载体系统诱导性表达感兴趣的多肽，包括：i）。第一载体包含：（a）位于诱导型启动子5'的T7启动子; （b）位于诱导型启动子3'的克隆位点，用于插入编码目标多肽的基因; 和ii）。用于制备用于T7RNA聚合酶表达的宿主细胞的第二载体，其中所述载体包含编码在诱导型启动子控制下的T7RNA聚合酶的基因。

5. 发明申请

WO2011073956A2 HETEROLOGOUS HOSTS 审中-公开
标题翻译：异物宿主
公开(公告)号：WO2011073956A2
公开(公告)日：2011-06-23
申请号：PCT/IB2010/055923
申请日：2010-12-17
申请人： GENE BRIDGES GMBH , ZHANG, Youming
发明人： ZHANG, Youming
IPC分类号： C12P17/10
CPC分类号： C12N15/70 , A61K35/74 , A61K2035/11 , C07D245/02 , C07D417/06 , C07D493/04 , C07D498/22 , C07G17/00 , C07K5/06139 , C07K7/64 , C12N9/1288 , C12N9/90 , C12N9/93 , C12N15/52 , C12N15/74 , C12P17/10 , C12P17/181 , C12Y207/08007 , Y02P20/52
摘要： This invention is related to bacterial engineering and the heterologous expression of useful compounds. In particular, the invention relates to a heterologous host that has been engineered for expression of a gene which is capable of polyketide or non- ribosomal peptide synthesis. Methods of treating cancer are also disclosed.
摘要翻译：本发明涉及细菌工程和有用化合物的异源表达。特别地，本发明涉及已被改造用于表达能够进行聚酮化合物或非核糖体肽合成的基因的异源宿主。还公开了治疗癌症的方法。

6. 发明申请

WO2010113031A3 METHOD OF ALTERING NUCLEIC ACIDS 审中-公开
公开(公告)号：WO2010113031A3
公开(公告)日：2010-10-07
申请号：PCT/IB2010/000893
申请日：2010-03-30
申请人： GENE BRIDGES GMBH , STEWART, Adrian, Francis , ZHANG, Youming , MARESCA, Marcello , KRANZ, Harald , NOLL, Stephan
发明人： STEWART, Adrian, Francis , ZHANG, Youming , MARESCA, Marcello , KRANZ, Harald , NOLL, Stephan
IPC分类号： C12N15/10 , C12N15/90
摘要： The invention provides a method for altering the sequence of a target nucleic acid molecule, said method comprising the steps of: a) introducing a nucleic acid fragment into the target nucleic acid molecule by homologous recombination, wherein the nucleic acid fragment comprises: i) a first region of homology to the target nucleic acid molecule; ii) a first recognition sequence for a Type IIS nuclease; iii) a selectable marker; iv) a second recognition sequence for a Type IIS nuclease; v) a second region of homology to the target nucleic acid molecule; wherein components i) to v) are ordered from 5' to 3'; and a replacement nucleic acid sequence positioned between the first and second regions of homology but flanking the recognition sequences for the Type IIS nucleases; b) selecting for the incorporation of the replacement nucleic acid using the selectable marker, and c) cleaving the product of step b) with Type IIS nucleases such that the selectable marker is excised, to produce a linear target fragment including the desired replacement sequence.

7. 发明申请

WO2002062988A2 RECOMBINATION METHOD 审中-公开
标题翻译：重组方法
公开(公告)号：WO2002062988A2
公开(公告)日：2002-08-15
申请号：PCT/IB2002/001415
申请日：2002-02-08
申请人： GENE BRIDGES GMBH , MUIJRERS, Joep, P., P. , ZHANG, Youming , STEWART, Adrian, Francis
发明人： MUIJRERS, Joep, P., P. , ZHANG, Youming , STEWART, Adrian, Francis
IPC分类号： C12N15/00
CPC分类号： C12N15/102
摘要： The invention relates to a novel method for altering the sequence of a nucleic acid molecule using repair recombination in a simple one component system. The frequency of the recombination reaction is high, allowing a range of feasible selection strategies to identify successful recombination events. The method involves the steps of bringing a first nucleic acid molecule into contact with a second nucleic acid molecule in the presence of a phage annealing protein into contact with a second nucleic acid molecule in the presence of a phage annealing protein, or a functional equivalent or fragment thereof, wherein said first nucleic acid molecule comprises at least two regions of shared sequence homology with the second nucleic acid molecule, under conditions suitable for repair recombination to occur between said first and second nucleic acid molecules; ad selecting a nucleic acid molecule whose sequence has been altered so as to include sequence from said second nucleic acid molecule.
摘要翻译：本发明涉及一种在简单的单一成分系统中使用修复重组改变核酸分子的序列的新方法。重组反应的频率很高，允许一系列可行的选择策略来鉴定成功的重组事件。该方法包括以下步骤：在噬菌体退火蛋白存在下，使第一核酸分子与第二核酸分子接触，以在噬菌体退火蛋白或功能等价物或存在下与第二核酸分子接触，其中所述第一核酸分子在适于在所述第一和第二核酸分子之间发生修复重组的条件下包含与所述第二核酸分子具有共享序列同源性的至少两个区域; 选择其序列已被改变以便包括来自所述第二核酸分子的序列的核酸分子。

8. 发明申请

WO2002062988A3 RECOMBINATION METHOD 审中-公开
公开(公告)号：WO2002062988A3
公开(公告)日：2002-08-15
申请号：PCT/IB2002/001415
申请日：2002-02-08
申请人： GENE BRIDGES GMBH , MUIJRERS, Joep, P., P. , ZHANG, Youming , STEWART, Adrian, Francis
发明人： MUIJRERS, Joep, P., P. , ZHANG, Youming , STEWART, Adrian, Francis
IPC分类号： C12N15/00
摘要： The invention relates to a novel method for altering the sequence of a nucleic acid molecule using repair recombination in a simple one component system. The frequency of the recombination reaction is high, allowing a range of feasible selection strategies to identify successful recombination events. The method involves the steps of bringing a first nucleic acid molecule into contact with a second nucleic acid molecule in the presence of a phage annealing protein into contact with a second nucleic acid molecule in the presence of a phage annealing protein, or a functional equivalent or fragment thereof, wherein said first nucleic acid molecule comprises at least two regions of shared sequence homology with the second nucleic acid molecule, under conditions suitable for repair recombination to occur between said first and second nucleic acid molecules; ad selecting a nucleic acid molecule whose sequence has been altered so as to include sequence from said second nucleic acid molecule.

9. 发明申请

WO2003004652A2 A NEW TYROSINE RECOMBINASE FOR GENETIC ENGINEERING 审中-公开
标题翻译：一种用于遗传工程的新型酪氨酸重组体
公开(公告)号：WO2003004652A2
公开(公告)日：2003-01-16
申请号：PCT/EP2002/007176
申请日：2002-06-28
申请人： THE EUROPEAN MOLECULAR BIOLOGY LABORATORY , L'UNIVERSITE CATHOLIQUE DE LOUVAIN , STEWART, Francis, A. , ZHANG, Youming , HALLET, Bernard
发明人： STEWART, Francis, A. , ZHANG, Youming , HALLET, Bernard
IPC分类号： C12N15/52
CPC分类号： C12N9/00 , C12N15/902
摘要： The present invention is directed to methods and compositions for TnpI-mediated genetic engineering using the Bacillus thurungiensis recombinase TnpI and the TnpI recombination substrates TRT or TRT', and variants thereof. In particular, the invention relates to TRT or TRT' sequences, and variants thereof, vectors, cells and kits useful for TnpI-mediated genetic recombination, as well as methods for the use of TRT or TRT' sequences for TnpI-mediated genetic engineering.
摘要翻译：本发明涉及使用芽孢杆菌Thrungiensis重组酶TnpI和TnpI重组底物TRT或TRT'及其变体的TnpI介导的遗传工程的方法和组合物。特别地，本发明涉及可用于TnpI介导的基因重组的TRT或TRT'序列及其变体，载体，细胞和试剂盒，以及用于TnpI介导的基因工程的TRT或TRT'序列的使用方法。

10. 发明申请

WO2003010322A1 IMPROVED RECT OR RECET CLONING AND SUBCLONING METHOD 审中-公开
标题翻译：改进的重建或恢复克隆和扩充方法
公开(公告)号：WO2003010322A1
公开(公告)日：2003-02-06
申请号：PCT/EP2002/008096
申请日：2002-07-19
申请人： ZHANG, Youming , STEWART, Francis, A. , MUIJRERS, Joep, P., P.
发明人： ZHANG, Youming , STEWART, Francis, A. , MUIJRERS, Joep, P., P.
IPC分类号： C12N15/90
CPC分类号： C12N15/64 , C12N15/66
摘要： The invention refers to an improved method for DNA cloning and subcloning using Rec T or RecET-mediated homologous recombination. Further, novel reagent kits suitable for carrying out the method are provided.
摘要翻译：本发明涉及使用Rec T或RecET介导的同源重组的DNA克隆和亚克隆的改进方法。此外，提供了适用于实施该方法的新型试剂盒。

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