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1. 发明申请

WO2008064067A3 METHOD FOR THE SEQUENTIAL ANALYSIS OF DIFFERENT COMPONENTS IN A SINGLE BIOLOGICAL SAMPLE AND REAGENTS THEREFOR 审中-公开
标题翻译：单一生物样品和试剂中不同成分的顺序分析方法
公开(公告)号：WO2008064067A3
公开(公告)日：2008-07-10
申请号：PCT/US2007084800
申请日：2007-11-15
申请人： GEN ELECTRIC , PANG ZHENGYU , FILKINS ROBERT JOHN , MONTALTO MICHAEL CHRISTOPHER , GINTY FIONA , GERDES MICHAEL J , CAN ALI , BRESNAHAN MAUREEN ANN , SOOD ANUP
发明人： PANG ZHENGYU , FILKINS ROBERT JOHN , MONTALTO MICHAEL CHRISTOPHER , GINTY FIONA , GERDES MICHAEL J , CAN ALI , BRESNAHAN MAUREEN ANN , SOOD ANUP
IPC分类号： G01N33/50 , C12Q1/68
CPC分类号： G01N33/582 , C12Q1/6816 , G01N33/542 , C12Q2545/101 , C12Q2527/125
摘要： Methods for probing multiple targets in a biological sample are provided. The methods include the steps of providing a biological sample containing multiple targets, binding at least one fluorescent probe to one or more target present in the sample, and binding at least at least one control probe to one or more target present in the sample. The methods include the steps of observing a signal from the fluorescent probe and a control signal from the control probe and applying to the sample a basic solution containing an oxidizing agent that selectively inactivates the fluorescent probe and not the control probe. The methods further include the steps of binding at least one fluorescent probe to one or more target present in the sample and observing a signal from the fluorescent probe. The methods disclosed herein also provide for multiple iterations of binding, observing, and oxidizing for deriving information about multiple targets in a single sample. An associated kit is also provided.
摘要翻译：提供了探索生物样品中多个靶标的方法。所述方法包括以下步骤：提供含有多个靶标的生物样品，将至少一种荧光探针结合至样品中存在的一种或多种靶标，以及将至少一种对照探针结合至样品中存在的一种或多种靶标。所述方法包括以下步骤：观察来自荧光探针的信号和来自对照探针的控制信号，并向样品施加含有选择性地使荧光探针失活而不是对照探针的氧化剂的碱性溶液。所述方法还包括将至少一种荧光探针结合到样品中存在的一种或多种目标物并观察来自荧光探针的信号的步骤。本文公开的方法还提供结合，观察和氧化的多次迭代，用于导出关于单个样品中的多个靶的信息。还提供了相关的套件。

2. 发明申请

WO2008133729A3 SEQUENTIAL ANALYSIS OF BIOLOGICAL SAMPLES WITH INTERMEDIATE BLEACHING OF FLUORESCENCE DETECTOR 审中-公开
标题翻译：生物样品的序贯分析与荧光检测器的中间漂白
公开(公告)号：WO2008133729A3
公开(公告)日：2009-01-08
申请号：PCT/US2007084791
申请日：2007-11-15
申请人： GEN ELECTRIC , SOOD ANUP , GERDES MICHAEL J , PANG ZHENGYU , GINTY FIONA , MONTALTO MICHAEL CHRISTOPHER , LARSEN MELINDA
发明人： SOOD ANUP , GERDES MICHAEL J , PANG ZHENGYU , GINTY FIONA , MONTALTO MICHAEL CHRISTOPHER , LARSEN MELINDA
IPC分类号： G01N33/68 , C12Q1/68
CPC分类号： G01N33/53 , C12Q1/68 , G01N33/542 , G01N33/582 , Y10T436/143333
摘要： Methods for probing multiple targets in a biological sample are provided. The methods include the steps of providing a biological sample containing multiple targets adhered to a solid support, binding at least one fluorescent probe to one or more target present in the sample, and observing a signal from the fluorescent probe. The method further includes the steps of oxidizing the bound fluorescent probe with a solution containing an oxidizing agent that substantially inactivates the fluorescent probe, binding at least one fluorescent probe to one or more target present in the sample, and observing a signal from the fluorescent probe. The methods disclosed herein also provide for multiple iterations of binding, observing, and oxidizing for deriving information about multiple targets in a single sample. An associated kit is also provided.
摘要翻译：提供了探测生物样品中多个靶标的方法。所述方法包括以下步骤：提供包含粘附到固体支持物上的多个靶的生物样品，将至少一个荧光探针结合到样品中存在的一个或多个靶，并观察来自荧光探针的信号。所述方法还包括以下步骤：用含有基本上灭活荧光探针的氧化剂的溶液氧化结合的荧光探针，将至少一种荧光探针结合到样品中存在的一种或多种靶，并观察来自荧光探针的信号。本文公开的方法还提供结合，观察和氧化的多次迭代，用于导出关于单个样本中的多个目标的信息。还提供了相关的套件。

3. 发明申请

WO2008021681A2 SYSTEM AND METHODS FOR ANALYZING IMAGES OF TISSUE SAMPLES 审中-公开
标题翻译：用于分析组织样本图像的系统和方法
公开(公告)号：WO2008021681A2
公开(公告)日：2008-02-21
申请号：PCT/US2007074388
申请日：2007-07-26
申请人： GEN ELECTRIC , FILKINS ROBERT JOHN , CLINE HARVEY ELLIS , MONTALTO MICHAEL CHRISTOPHER , ADAK SUDESHNA , CAN ALI , GERDES MICHAEL J , LARSEN MELINDA , GINTY FIONA
发明人： FILKINS ROBERT JOHN , CLINE HARVEY ELLIS , MONTALTO MICHAEL CHRISTOPHER , ADAK SUDESHNA , CAN ALI , GERDES MICHAEL J , LARSEN MELINDA , GINTY FIONA
CPC分类号： G06T7/0012
摘要： A system for analyzing tissue samples, comprising: a storage device for at least temporarily storing one or more images of one or more cells, wherein at least one of the images is indicative of one or more channels comprising a receptor tyrosine kinase (RTK); and a processing device that determines an extent to which one or more of the RTKs may have translocated from at least one subcellular region to another subcellular region of one or more of the cells; and generates a score based at least in part on the RTK translocation.
摘要翻译：一种用于分析组织样本的系统，包括：用于至少临时存储一个或多个细胞的一个或多个图像的存储装置，其中所述图像中的至少一个指示包含受体酪氨酸激酶（RTK）的一个或多个通道; 以及处理设备，其确定一个或多个RTK可以从一个或多个单元的至少一个亚细胞区域移位到另一个亚细胞区域的程度; 并且至少部分地基于RTK易位产生得分。

4. 发明申请

WO2008133727A3 SEQUENTIAL ANALYSIS OF BIOLOGICAL SAMPLES WITH BLEACHING OF INTERMEDIATE FLUORESCENT SIGNALS 审中-公开
标题翻译：中间荧光信号漂白生物样品的顺序分析
公开(公告)号：WO2008133727A3
公开(公告)日：2009-01-22
申请号：PCT/US2007084783
申请日：2007-11-15
申请人： GEN ELECTRIC , FILKINS ROBERT JOHN , MONTALTO MICHAEL CHRISTOPHER , SOOD ANUP , GERDES MICHAEL J , SEEL MAXIMILIAN LEWIS
发明人： FILKINS ROBERT JOHN , MONTALTO MICHAEL CHRISTOPHER , SOOD ANUP , GERDES MICHAEL J , SEEL MAXIMILIAN LEWIS
IPC分类号： G01N33/542
CPC分类号： G01N33/53 , C12Q1/68 , G01N33/542 , G01N33/582 , Y10T436/143333
摘要： Methods for detecting multiple targets in a biological sample are provided. The methods includes contacting the sample with a first probe; physically binding the first probe to a first target; observing a first signal from the first probe; applying a chemical agent to modify the first signal; contacting the sample with a second probe; physically binding the second probe to a second target; and observing a second signal from the second probe. The methods disclosed herein also provide for multiple iterations of binding, observing, signal modification for deriving information about multiple targets in a single sample. An associated kit and device are also provided.
摘要翻译：提供了用于检测生物样品中多个靶的方法。所述方法包括使样品与第一探针接触; 将第一探针物理地绑定到第一靶; 观察来自第一探针的第一信号; 施用化学试剂来修饰第一信号; 使样品与第二探针接触; 将第二探针物理地绑定到第二靶上; 并观察来自第二探测器的第二信号。本文公开的方法还提供绑定，观察，信号修改的多次迭代，用于导出关于单个样本中的多个目标的信息。还提供了相关的套件和设备。

5. 发明申请

WO2008133728A3 SEQUENTIAL ANALYSIS OF BIOLOGICAL SAMPLES WITH INTERMEDIATE BLEACHING OF FLUORESCENCE DETECTOR 审中-公开
标题翻译：生物样品的序贯分析与荧光检测器的中间漂白
公开(公告)号：WO2008133728A3
公开(公告)日：2009-01-22
申请号：PCT/US2007084786
申请日：2007-11-15
申请人： GEN ELECTRIC , SOOD ANUP , GERDES MICHAEL J , TREYNOR THOMAS PIRRIE , PANG ZHENGYU
发明人： SOOD ANUP , GERDES MICHAEL J , TREYNOR THOMAS PIRRIE , PANG ZHENGYU
IPC分类号： G01N33/68 , C12Q1/68
CPC分类号： G01N33/581 , G01N33/6803
摘要： Methods for probing multiple targets in a biological sample are provided. The methods include the steps of providing a sample containing multiple targets, binding at least one probe having a binder coupled to an enzyme to one or more target present in the sample, and reacting the bound probe with an enzyme substrate coupled to a fluorescent signal generator. The methods include the steps of observing a signal from the fluorescent signal generator and applying to the sample a solution containing an oxidizing agent that substantially inactivates both the fluorescent signal generator and the enzyme. The methods further include the steps of binding at least one probe having a binder coupled to an enzyme to one or more target present in the sample of step, reacting the bound probe with an enzyme substrate coupled to a fluorescent signal generator; and observing a signal from the fluorescent signal generator. The methods disclosed herein also provide for multiple iterations of binding, observing, and oxidizing for deriving information about multiple targets in a single sample. An associated kit is also provided.
摘要翻译：提供了探测生物样品中多个靶标的方法。所述方法包括以下步骤：提供含有多个靶标的样品，将至少一种具有与酶偶联的结合剂的探针结合至样品中存在的一种或多种靶标，以及使结合的探针与偶联于荧光信号发生器。所述方法包括以下步骤：观察来自荧光信号发生器的信号，并向样品施加含有基本上灭活荧光信号发生器和酶的氧化剂的溶液。所述方法还包括以下步骤：将具有与酶偶联的粘合剂的至少一种探针与步骤样品中存在的一种或多种靶结合，使结合的探针与耦合到荧光信号发生器的酶底物反应; 并观察来自荧光信号发生器的信号。本文公开的方法还提供结合，观察和氧化的多次迭代，用于导出关于单个样本中的多个目标的信息。还提供了相关的套件。

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