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1. 发明申请

WO0222799A3 METHOD FOR THE MICROBIAL PRODUCTION OF METABOLIC PRODUCTS, POLYNUCLEOTIDES FROM CORYNEFORM BACTERIA AND USE THEREOF 审中-公开
标题翻译：用于生产微生物代谢产物，从杆状菌及其用途多核苷酸
公开(公告)号：WO0222799A3
公开(公告)日：2003-03-13
申请号：PCT/DE0103485
申请日：2001-09-08
申请人： FORSCHUNGSZENTRUM JUELICH GMBH , DEGUSSA , BOTT MICHAEL , NIEBISCH AXEL , BATHE BRIGITTE , MARX ACHIM , HERMANN THOMAS
发明人： BOTT MICHAEL , NIEBISCH AXEL , BATHE BRIGITTE , MARX ACHIM , HERMANN THOMAS
IPC分类号： C07K14/34 , C12N1/21 , C12N9/02 , C12N15/31 , C12N15/52 , C12N15/53 , C12P1/04 , C12P7/04 , C12P7/40 , C12P13/04 , C12P19/38 , C12Q1/68
CPC分类号： C12N9/0036 , C07K14/34 , C12N9/0038 , C12N9/0053 , C12N15/52 , C12P1/04
摘要： The invention relates to a method for the microbial production ofmetabolic products, polynucleotides from coryneform bacteria and use thereof. According to the invention, by means of said method and polynucleotides it is possible to influence the synthesis of ATP in a controlled manner and also to control the synthesis of metabolic products. The invention relates to genes from Corynebacterium glutamicum coding for cytochrome aa3 oxidase and the cytochrome bcl complex. The monocistronic ctaD gene codes for a 65 kDa protein, the primary structure of which displays all the typical properties of the sub-unit I of cytochrome aa3 oxidase. The genes which code for the sub-unit III of the cytochrome aa3 (ctaE) and the three characteristic sub-units of the cytochrome bc1 complex (qcrABC) are arranged in a group with the sequence ctaE-qcrCAB.
摘要翻译：本发明涉及一种用于从棒状细菌和它们的使用的微生物生产的代谢产物，多核苷酸的方法。本发明的方法和多核苷酸，它是现在可以影响ATP的合成目标，从而控制代谢产物的合成。本发明涉及一种细胞色素氧化酶AA3和谷氨酸棒杆菌细胞色素BC1复杂的编码基因。单顺反子基因编码CTAD大的蛋白质，其主要结构具有细胞色素氧化酶AA3的亚基我为65 kDa的所有的典型特征。编码细胞色素BC1络合物（qcrABC）被布置的顺序ctaE-qcrCAB一组中的特性编码细胞色素AA3（ctaE）的亚基III的基因和三个亚基。

2. 发明申请

WO2007042494A2 MICROBIOLOGICAL PRODUCTION OF 3-HYDROXYPROPIONIC ACID 审中-公开
标题翻译： 3-羟基丙酸的微生物制备
公开(公告)号：WO2007042494A2
公开(公告)日：2007-04-19
申请号：PCT/EP2006067182
申请日：2006-10-09
申请人： DEGUSSA , FORSCHUNGSZENTRUM JUELICH GMBH , MARX ACHIM , WENDISCH VOLKER F , RITTMANN DORIS , BUCHHOLZ STEFAN
发明人： MARX ACHIM , WENDISCH VOLKER F , RITTMANN DORIS , BUCHHOLZ STEFAN
IPC分类号： C12N15/52 , C12P7/18 , C12P7/40 , C12P13/06
CPC分类号： C12P7/40 , C12P7/42 , C12P7/44
摘要： The invention relates to a cell which is genetically modified in relation to a wild type and exhibits at least one following properties: a) the activity of an enzyme E 1 , which is used for catalysing the conversion of pyruvate into oxalacetate or of an enzyme E 1b for catalysing the conversion of phosphoenolpyruvate into oxalacetate, and which is greater than the wild type activity and b) the activity of an enzyme E 2 , which is used for catalysing the conversion of aspartate into beta-alanine and which is greater than the wild type activity, wherein the inventive cell, in addition to the a) and b) properties, is characterised in that it exhibits at least one following property: c) the genetically modified cell is capable to eject beta-alanine therefrom and d) the genetically modified cell is capable to convert the beta-alanine into the 3-hydrixypropionic acid. A method for producing a genetically modified cell, the genetically modified cells obtainable by said method, a method for producing the 3-hydrixypropionic acid, a method for producing an acrylic acid, a method for producing polyacrylates, a method for producing acrylic acid esters and the use of cells for producing the 3-hydrixypropionic acid are also disclosed.
摘要翻译：本发明涉及一种比其野生型遗传修饰的细胞，其包含的特征a）或b）至少一种的：相比于它的野生型，酶E _{1 的，该方法包括使一）催化丙酮酸成草酰乙酸，或酶E _{图1b ，其催化磷酸烯醇丙酮酸转化成草酰乙酸的，b）相比，其野生型，酶E的_{2 ，这催化天冬氨酸向β-丙氨酸的转化，其中，除了a的属性）或b）由所述性质中的至少一种）或d）的特征在于c中的细胞：c）中经遗传修饰的细胞能够从β-丙氨酸去除细胞; d）经遗传修饰的细胞能够将β-丙氨酸转化为3-羟基丙酸。本发明还涉及一种用于制造经遗传修饰的细胞的方法，通过该方法获得的遗传修饰的细胞，产生3-羟基丙酸，为生产丙烯酸的过程中，聚丙烯酸酯的制备方法，用于Acrylsäureestern的制备方法的方法和细胞用于生产3-羟基丙酸的用途。}}}

3. 发明申请

WO2007141208A3 MICROBIOLOGICAL PRODUCTION OF 3-HYDROXYISOBUTYRIC ACID 审中-公开
标题翻译：微生物制剂3-羟基异
公开(公告)号：WO2007141208A3
公开(公告)日：2008-03-20
申请号：PCT/EP2007055394
申请日：2007-06-01
申请人： EVONIK DEGUSSA GMBH , UNIV ALBERT LUDWIGS FREIBURG , MARX ACHIM , POETTER MARKUS , BUCHHOLZ STEFAN , MAY ALEXANDER , SIEGERT HERMANN , ALBER BIRGIT , FUCHS GEORG , EGGELING LOTHAR
发明人： MARX ACHIM , POETTER MARKUS , BUCHHOLZ STEFAN , MAY ALEXANDER , SIEGERT HERMANN , ALBER BIRGIT , FUCHS GEORG , EGGELING LOTHAR
IPC分类号： C12P7/42 , C07C57/04 , C12N9/02 , C12P7/52
CPC分类号： C12P7/42 , C08F120/06 , C08F120/10 , C12N9/0008 , C12N9/001 , C12N9/1096 , C12N9/16 , C12N9/88 , C12P7/40 , C12P7/52 , C12P7/625 , C12Y102/04004 , C12Y103/99012 , C12Y206/01042 , C12Y301/02004 , C12Y402/01017
摘要： The present invention relates to a cell which has been genetically modified by comparison with its wild type in such a way that it is able to form more, by comparison with its wild type, 3-hydroxyisobutyric acid or polyhydroxyalkanoates based on 3-hydroxyisobutyric acid via methylmalonate semialdehyde or 3-hydroxybutyryl-coenzyme A as precursors. The invention also relates to a method for producing a genetically modified cell, to the genetically modified cell obtainable by this method, to a method for producing 3-hydroxyisobutyric acid or polyhydroxyalkanoates based on 3-hydroxyisobutyric acid, to a method for producing methacrylic acid or methacrylic esters, and to a method for producing polymethacrylic acid or polymethacrylic esters. The present invention further relates to an isolated DNA, to a vector, to the use of this vector for transforming a cell, to a transformed cell, and to a polypeptide.
摘要翻译：是能够形成作为前体，本发明涉及已经被改变在这样的基因相比，它的野生型，即，相对于它的野生型更3-羟基异丁酸或基于3-羟基聚羟基烷酸酯甲基丙二酸半醛或3-羟基丁酰基辅酶A细胞，本发明还涉及一种方法，用于通过该方法生产遗传修饰的细胞，获得遗传修饰的细胞，为3-羟基异丁酸的制备方法或基于3-羟基异丁酸，用于生产甲基丙烯酸或甲基丙烯酸酯的方法，和一种方法对于聚甲基丙烯酸或聚甲基丙烯酸的制备。本发明还涉及一种分离的DNA，载体，用于细胞，转化的细胞的转化使用这种载体的，和多肽。

4. 发明申请

WO2011157573A3 AN ENZYME FOR THE PREPARATION OF METHYLMALONATE SEMIALDEHYDE 审中-公开
标题翻译： AN酶甲基丙二酸二乙酯生产
公开(公告)号：WO2011157573A3
公开(公告)日：2012-04-26
申请号：PCT/EP2011059178
申请日：2011-06-03
申请人： EVONIK ROEHM GMBH , POETTER MARKUS , MARX ACHIM , SCHAFFER STEFFEN , REINECKE LIV , HAAS THOMAS
发明人： POETTER MARKUS , MARX ACHIM , SCHAFFER STEFFEN , REINECKE LIV , HAAS THOMAS
IPC分类号： C12N9/02 , C12N5/10
CPC分类号： C12P7/62 , C12N9/001
摘要： The subject matter of the invention is an enzyme for the preparation of methylmalonate semialdehyde or malonate semialdehyde, nucleic acids comprising coding sequences for this enzyme, cells which have been transformed with these nucleic acids, and the use of this enzyme.
摘要翻译：本发明是一种用于生产甲基丙或丙二酸半醛，核酸，其包含编码这种酶的序列，这些核酸，如使用这种酶的转化细胞的酶。

5. 发明申请

WO2010000506A3 METHOD FOR THE PRODUCTION OF FREE CARBOXYLIC ACIDS 审中-公开
标题翻译：用于生产无碳氢酸
公开(公告)号：WO2010000506A3
公开(公告)日：2010-02-25
申请号：PCT/EP2009055163
申请日：2009-04-29
申请人： EVONIK ROEHM GMBH , HAAS THOMAS , TACKE THOMAS , MARX ACHIM , SCHRAVEN ALEXANDER , ZEHNACKER OLIVIER , WITTMANN EVA-MARIA
发明人： HAAS THOMAS , TACKE THOMAS , MARX ACHIM , SCHRAVEN ALEXANDER , ZEHNACKER OLIVIER , WITTMANN EVA-MARIA
IPC分类号： C12P7/40 , C07C51/48 , C12P7/42 , C12P7/52
CPC分类号： C12P7/40 , C07C51/02 , C07C51/41 , C07C51/47 , C12P7/42 , C07C59/01 , C07C59/06 , C07C59/255 , C07C59/08 , C07C59/265
摘要： The invention relates to a method for producing free carboxylic acids, comprising the following method steps: A) production of the carboxylic acid by a biological cell present in an aqueous medium by adding an amine of general formula (I) in which R1, R2 and R3 independently of one another are the same or different, branched or unbranched, optionally substituted hydrocarbon radicals or H; B) in case the amine added in method step A) is water-soluble, addition of a water-insoluble amine of general formula (I), wherein a multiphase system is obtained in method step A) or B) and the water-insoluble amine and the carboxylic acid produce the corresponding ammonium carboxylate; C) separation of the water-insoluble phase; and D) heating the water-insoluble phase while releasing free carboxylic acid.
摘要翻译：本发明涉及一种用于生产游离羧酸的方法，包括该过程本身在水性介质中利用Dende步骤a）羧酸的制备中，在加入通式（I）的胺，其中R1，R2和R3独立地是相同的或生物细胞是不相等的，支链或无支链的，任选地取代的烃基或H，B）对于所加入的胺可溶于在工艺步骤a）水，加入通式（I）的不溶于水的胺的情况下，其中在所述方法步骤a）或b）获得多相体系和以形成水不溶性胺的相应的羧酸铵和羧酸，以及C）分离所述水不溶相和D）具有游离羧酸的解放加热水不溶相。

6. 发明申请

WO2011057871A3 USE OF A PROTEIN HOMOLOGOUS TO A MEAB PROTEIN FOR INCREASING THE ENZYMATIC ACTIVITY OF A 3-HYDROXYCARBOXYLIC ACID-COA MUTASE 审中-公开
标题翻译： AN MEAB蛋白同源蛋白质的FOR A 3-羟基酰基-CoA变位酶增加酶活性USE
公开(公告)号：WO2011057871A3
公开(公告)日：2012-01-26
申请号：PCT/EP2010065151
申请日：2010-10-11
申请人： EVONIK ROEHM GMBH , REINECKE LIV , SCHAFFER STEFFEN , KOEHLER TIM , THIESSENHUSEN ANJA , MARX ACHIM , BUCHHAUPT MARKUS
发明人： REINECKE LIV , SCHAFFER STEFFEN , KOEHLER TIM , THIESSENHUSEN ANJA , MARX ACHIM , BUCHHAUPT MARKUS
IPC分类号： C07K14/47
CPC分类号： C12P7/52 , C07K14/195 , C07K2319/00 , C12N9/90 , C12P7/42
摘要： The invention relates to the use of a protein homologous to a MeaB protein for increasing the enzymatic activity of a 3-hydroxycarboxylic acid-CoA mutase, a fusion protein comprising a 3-hydroxycarboxylic acid-CoA mutase and a protein sequence homologous to a MeaB protein and an enzymatic method for producing 2-hydroxyisobutryric acid.
摘要翻译：本发明涉及使用一种同源MEAB蛋白蛋白质的用于增加3-羟基酰基-CoA变位酶的酶活性的融合蛋白，其包括3-羟基酰基-CoA变位酶，和同源MeaB-蛋白质蛋白质序列和用于制备2-羟基异丁酸酶促过程。

7. 发明申请

WO0234775A2 NUCLEOTIDE SEQUENCES ENCODING THE HEMD AND HEMB GENES 审中-公开
标题翻译：编码HEMD和HEMB基因的核苷酸序列
公开(公告)号：WO0234775A2
公开(公告)日：2002-05-02
申请号：PCT/EP0111705
申请日：2001-10-10
申请人： DEGUSSA
发明人： FARWICK MIKE , HUTHMACHER KLAUS , PFEFFERLE WALTER , SCHISCHKA NATALIE , MARX ACHIM
IPC分类号： C12N9/88 , C12P13/08 , C07K14/34 , C12N9/00 , C12N9/10 , C12N15/10 , C12N15/63 , C12Q1/68
CPC分类号： C12P13/08 , C12N9/88 , C12Y402/01024 , C12Y402/01075
摘要： The invention relates to an isolated polynucleotide containing a polynucleotide sequence, selected from the group a) polynucleotide that is at least 70 % identical with a polynucleotide encoding a polypeptide that contains the amino acid sequence of SEQ ID No. 2, b) polynucleotide that is at least 70 % identical with a polynucleotide encoding a polypeptide that contains the amino acid sequence of SEQ ID No. 3, c) polynucleotide encoding a polypeptide that contains an amino acid sequence that is at least 70 % identical with the amino acid sequence of SEQ ID No. 2, d) polynucleotide encoding a polypeptide that contains an amino acid sequence that is at least 70 % identical with the amino acid sequence of SEQ ID No. 3, e) polynucleotide that is complementary to the polynucleotides of a), b), c) and d), and f) polynucleotide containing at least 15 consecutive nucleotides of the polynucleotide sequence of a), b), c), d) and e), and a method for the fermentative production of L-amino acids using coryneform bacteria, in which at least the hemD-gene and/or the hemB-gene is/are amplified, and the use, as Hybridization probes, of polynucleotides that contain the sequences according to the invention.
摘要翻译：本发明涉及含有多核苷酸序列的分离的多核苷酸，所述多核苷酸序列选自：a）与编码含有SEQ ID No.2的氨基酸序列的多肽的多核苷酸至少70％相同的多核苷酸，b）与编码含有SEQ ID No.3的氨基酸序列的多肽的多核苷酸至少70％相同，c）编码含有与SEQ ID No.3的氨基酸序列至少70％相同的氨基酸序列的多肽的多核苷酸 d）编码多肽的多核苷酸，所述多肽含有与SEQ ID No.3的氨基酸序列至少70％相同的氨基酸序列，e）与a）的多核苷酸互补的多核苷酸，b ），c）和d），和f）含有a），b），c），d）和e）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及用于发酵生产L-氨基酸 ü 其中至少扩增hemD-基因和/或hemB-基因的棒状杆菌细菌，以及使用含有本发明序列的多核苷酸作为杂交探针。

8. 发明申请

WO0194569A2 NOVEL NUCLEOTIDE SEQUENCES CODING FOR THE GLBO GENE 审中-公开
标题翻译： GLBO基因的新核苷酸序列编码
公开(公告)号：WO0194569A2
公开(公告)日：2001-12-13
申请号：PCT/EP0104792
申请日：2001-04-27
申请人： DEGUSSA
发明人： MOECKEL BETTINA , MARX ACHIM , PFEFFERLE WALTER
IPC分类号： C07K14/34 , C07K14/805 , C12N1/21 , C12P13/04 , C12P13/08 , C12N15/00
CPC分类号： C12P13/04 , C07K14/34 , C07K14/805 , C12P13/08
摘要： The present invention provides an isolated polynucleotide containing a polynucleotide sequence selected from the group a) polynucleotide which is at least 70% identical to a polynucleotide which codes for a polypeptide containing the amino acid sequence of SEQ ID no. 2, b) polynucleotide which codes for a polypeptide which contains an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID no. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide containing at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative production of L-amino acids with enhancement of the glbO gene which codes for the haemoglobin-like protein and the use of the above polynucleotides as a primer or hybridization probe.
摘要翻译：本发明提供了一种分离的多核苷酸，其含有多核苷酸序列，该多核苷酸序列选自a）多核苷酸，该多核苷酸与编码含有SEQ ID NO：1的氨基酸序列的多肽的多核苷酸具有至少70％的相同性。 2，b）编码多肽的多核苷酸，其含有与SEQ ID No：1的氨基酸序列至少70％相同的氨基酸序列。 2，c）与a）或b）的多核苷酸互补的多核苷酸，以及d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及d）发酵生产具有编码血红蛋白样蛋白质的glbO基因增强的L-氨基酸和使用上述多核苷酸作为引物或杂交探针。

9. 发明申请

WO0220572A2 NUCLEOTIDE SEQUENCES CODING FOR THE CHRS GENE 审中-公开
标题翻译：核糖体序列编码CHRS基因
公开(公告)号：WO0220572A2
公开(公告)日：2002-03-14
申请号：PCT/EP0109096
申请日：2001-08-07
申请人： DEGUSSA
发明人： BATHE BRIGITTE , SCHISCHKA NATALIE , MARX ACHIM , PFEFFERLE WALTER
IPC分类号： C07K14/34 , C12N9/12 , C12N15/54 , C12P13/08 , C07K14/195
CPC分类号： C12N9/1223 , A01K2217/075 , C07K14/34 , C12P13/08
摘要： The invention provides an isolated polynucleotide containing a polynucleotide sequence chosen from the group consisting of a) a polynucleotide which is at least 70% identical to a polynucleotide which codes for a polypeptide which contains the amino acid sequence in SEQ ID No. 2, b) a polynucleotide which codes for a polypeptide which contains an amino acid sequence which is at least 70% identical to the amino acid sequence in SEQ ID No. 2, c) a polynucleotide which is complementary to the polynucleotides in a) or b), and d) a polynucleotide containing a sequence of at least 15 consecutive nucleotides from the polynucleotide sequence in a), b) or c),and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the chrS gene present is attenuated, and the use of polynucleotides which contain sequences according to the invention as hybridization probes.
摘要翻译：本发明提供了一种分离的多核苷酸，其含有多核苷酸序列，所述多核苷酸序列选自a）与编码含有SEQ ID No.2所示氨基酸序列的多肽的多核苷酸至少70％相同的多核苷酸，b）编码多肽的多核苷酸，其含有与SEQ ID No.2中的氨基酸序列至少70％相同的氨基酸序列，c）与a）或b）中的多核苷酸互补的多核苷酸，以及 d）含有a），b）或c）中多核苷酸序列至少15个连续核苷酸序列的多核苷酸，以及使用至少存在chrS基因的棒状细菌发酵制备L-氨基酸的方法并且使用含有根据本发明的序列的多核苷酸作为杂交探针。

10. 发明申请

WO0222666A3 NUCLEOTIDE SEQUENCES WHICH CODE FOR THE GORA GENE 审中-公开
标题翻译：核苷酸序列，其代码为GORA基因
公开(公告)号：WO0222666A3
公开(公告)日：2002-07-25
申请号：PCT/EP0109314
申请日：2001-08-11
申请人： DEGUSSA
发明人： FARWICK MIKE , HUTHMACHER KLAUS , PFEFFERLE WALTER , MARX ACHIM
IPC分类号： C12N9/02 , C12P13/08 , C12N15/53 , C12N5/10 , C12P13/04 , C12Q1/68 , C12R1/15
CPC分类号： C12N9/0036 , C12P13/08
摘要： The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the gorA gene is present in attenuated form, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
摘要翻译：本发明涉及一种分离的多核苷酸，其包含多核苷酸序列，所述多核苷酸序列选自a）多核苷酸，其与编码多肽的多核苷酸的程度相同，所述多核苷酸包含SEQ ID No的氨基酸序列 2，b）编码多肽的多核苷酸，其包含与至少70％与SEQ ID No.2的氨基酸序列相同程度的氨基酸序列，c）多核苷酸，其与）或b），和d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少gorA 基因以减毒形式存在，并且使用包含根据本发明的序列的多核苷酸作为杂交探针。

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