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1. 发明申请

WO0056875A9 DETECTION, CLONING AND SEQUENCING OF POLYPEPTIDES WHICH DRIVE THE SUBCELLULAR LOCALIZATION OF PROTEINS 审中-公开
标题翻译：检测，克隆和测序推动蛋白质的细胞定位的多肽
公开(公告)号：WO0056875A9
公开(公告)日：2002-02-28
申请号：PCT/EP0002607
申请日：2000-03-23
申请人： EUROP LAB MOLEKULARBIOLOG , GONZALEZ CAYSTANO , BEJARANO LUIS
发明人： GONZALEZ CAYSTANO , BEJARANO LUIS
IPC分类号： C07K14/435 , C12N15/10 , C12N15/12 , C12N15/62
CPC分类号： C12N15/1051 , C07K2319/00 , C07K2319/60 , C12N15/1086
摘要： The present invention concerns a process for the detection, cloning and/or sequencing of polypeptides or parts thereof, which drive the subcellular localization of a protein containing such polypeptide or part thereof, characterized in that the process comprises the following steps: (a) constructing an expression library of random nucleic acids ligated to a reporter gene and contained in a vector molecule, (b) transfecting a plurality of host cells with the library, (c) screening for the subcellular localization of the expression product of the nucleic acid in the host cells via detection of a signal produced by the reporter gene, (d) cloning such cells where the reporter gene signal is detected in a certain subcellular localization, and (e) cloning and optionally sequencing the nucleic acid insert which encodes the polypeptide or part thereof. Polypeptides, driving the intracellular localization can be used to construct fusion proteins with predetermined intracellular localization.
摘要翻译：本发明涉及用于检测，克隆和/或测序多肽或其部分的方法，其驱动含有此类多肽或其部分的蛋白质的亚细胞定位，其特征在于该方法包括以下步骤：（a）构建连接到报告基因并包含在载体分子中的随机核酸的表达文库，（b）用该文库转染多个宿主细胞，（c）筛选核酸表达产物的亚细胞定位通过检测由报告基因产生的信号的宿主细胞，（d）克隆这样的细胞，其中在某些亚细胞定位中检测到报告基因信号，和（e）克隆和任选测序编码多肽或部分的核酸插入片段它们。驱动细胞内定位的多肽可用于构建具有预定细胞内定位的融合蛋白。

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