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    • 6. 发明申请
    • METHOD AND APPARATUS DETERMINING THE ISOELECTRIC POINT OF CHARGED ANALYTE
    • 方法和装置确定充电分析仪的电压点
    • WO2004065937A2
    • 2004-08-05
    • PCT/US2004/000630
    • 2004-01-12
    • PROTASIS CORPORATIONSTRAND, DavidLEATZOW, Dan, M.
    • STRAND, DavidLEATZOW, Dan, M.
    • G01N
    • B01D61/425C07K1/28G01N27/44704
    • Devices are provided for determining the isoelectric point of a charged analyte, comprising a titration chamber and an electrode chamber. The electrode chamber comprises at least two electrodes, for example, an electrode array. Either or both of the titration chamber and the electrode chamber may have a shaped geometry. The electrodes are operative, in conjunction with the shaped geometry of the chamber(s) where appropriate, to generate an electric field gradient in the titration chamber. Permeable material separates the titration chamber and the electrode chamber. A pH Sensor is located in the titration chamber for obtaining the pH of the first fluid. Certain preferred embodiments further include an analyte band detector for detecting the presence and optionally the location of a focused band of charged solute. Methods are provided for determining the isoelectric point of a charged analyte comprising introducing a carrier fluid comprising a Charge analyte into the titration chamber of a device as just described and applying an electric field gradient to focus the charged analyte into a focused band. The pH of the carrier fluid is incremented or adjusted to shift the location of the focused band of charged analyte, and the pH and location of the focused band of charged analyte are obtained for a plurality of locations and pH's and the isoelectric point is determined from such data.
    • 提供了用于确定带电分析物的等电点的装置,包括滴定室和电极室。 电极室包括至少两个电极,例如电极阵列。 滴定室和电极室中的任一个或两者可以具有成形几何形状。 电极在适当的情况下与室的成形几何结合起作用,以在滴定室中产生电场梯度。 渗透材料分离滴定室和电极室。 pH传感器位于滴定室中以获得第一流体的pH。 某些优选实施例还包括用于检测带电溶质的聚焦带的存在和可选地位置的分析物带检测器。 提供了用于确定带电分析物的等电点的方法,包括将包含电荷分析物的载体流体引入如上所述的装置的滴定室中,并施加电场梯度以将带电分析物聚焦到聚焦带中。 载体流体的pH被递增或调整以移动带电分析物的聚焦带的位置,并且对于多个位置和pH值获得带电分析物的聚焦带的pH和位置,并且等电点由 这样的数据。