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    • 3. 发明申请
    • SYSTEMS TO REDUCE RECALCITRANCE OF CELLULOSIC BIOMASS AND INCREASE YIELDS OF FERMENTABLE SUGARS
    • 减少细胞生物量回收和增加可食用糖的系统
    • WO2011160050A3
    • 2012-06-28
    • PCT/US2011040928
    • 2011-06-17
    • EDENSPACE SYSTEMS CORPPAPPAN KIRKBLAYLOCK MICHAELLEE DAVIDFERGUSON BRUCE
    • PAPPAN KIRKBLAYLOCK MICHAELLEE DAVIDFERGUSON BRUCE
    • A01H5/00C12N15/55C12N15/82C12P19/12
    • C12N9/00C12N9/18C12N9/2437C12N15/8255C12P19/02C12P19/14C12P2201/00C12Y302/01091
    • Described herein are methods to reduce the resistance of the plant cell wall to hydrolysis, methods to enhance the conversion of plant biomass into fermentable sugars, methods to increase yields of fermentable sugars from corn grain hydrolysis, and techniques to harvest both the grain and stover from the field simultaneously for hydrolysis. In certain embodiments, the invention provides transgenic plants transformed with expression vectors containing a DNA sequence encoding plant cell wall-modifying enzymes from microbial and animal sources. In certain embodiments, the invention provides methods of improving enzyme performance by creating variants of the parental enzyme. In certain embodiments, the invention provides methods of using stay-green varieties as biomass feedstocks for cellulosic biofuel production. Uses of this invention include, but are not limited to, enhanced biomass conversion, cost-effective lignocellulosic biomass conversion and biofuel production, reduced biomass recalcitrance, enhanced forage digestibility by animals, improved fiber qualities, and enhanced fiber processing properties.
    • 本文描述的是减少植物细胞壁对水解的抗性的方法,增强植物生物质转化为可发酵糖的方法,从玉米粒水解中提高可发酵糖产量的方法,以及收获谷物和秸秆两者的技术 该领域同时进行水解。 在某些实施方案中,本发明提供用含有编码来自微生物和动物来源的植物细胞壁修饰酶的DNA序列的表达载体转化的转基因植物。 在某些实施方案中,本发明提供了通过产生亲本酶的变体来提高酶性能的方法。 在某些实施方案中,本发明提供了使用保留绿色品种作为纤维素生物燃料生产的生物质原料的方法。 本发明的用途包括但不限于增强的生物质转化,成本有效的木质纤维素生物质转化和生物燃料生产,降低的生物质耐受性,动物提高的饲料消化率,改善的纤维品质和增强的纤维加工性能。