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    • 1. 发明申请
    • PROCESS AND APPARATUS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL AND FERMENTATION OF SUGARS
    • 木质纤维素材料的酶水解和糖的发酵的方法和装置
    • WO2015075277A1
    • 2015-05-28
    • PCT/EP2015/051839
    • 2015-01-29
    • DSM IP ASSETS B.V.
    • NOORDAM, BertusBERKHOUT, Michael Petrus JozefHOFMEESTER, Joseph Johannes Maria
    • C12P19/02D21C3/26C12P19/14C12M1/00
    • C12P19/02C12M45/06C12P19/14C12P2201/00C12P2203/00D21C3/263D21C5/005
    • The invention relates to a process for the preparation of a sugar product from lignocellulosic material, comprising the following steps: a) optionally, pretreatment of the lignocellulosic material; b) optionally, washing of the optionally pretreated lignocellulosic material; c) enzymatic hydrolysis of the optionally washed and/or optionally pretreated lignocellulosic material in a hydrolysis reactor using an enzyme composition comprising at least two cellulase; and d) optionally, recovery of a sugar product; wherein during the enzymatic hydrolysis oxygen-containing gas is added to the lignocellulosic material in the hydrolysis reactor and wherein part of the oxygen-containing gas, added to the lignocellulosic material, is gas originating from the headspace of the reactor, preferably during part of the time of the enzymatic hydrolysis less oxygen is added to the lignocellulosic material compared to the other part of the time of the enzymatic hydrolysis, or preferably during part of the time of the enzymatic hydrolysis no oxygen is added to the lignocellulosic material.
    • 本发明涉及一种从木质纤维素材料制备糖产品的方法,包括以下步骤:a)任选地预处理木质纤维素材料; b)任选地,任选地预处理的木质纤维素材料的洗涤; c)使用包含至少两种纤维素酶的酶组合物在水解反应器中酶解水解任选洗涤和/或任选预处理的木质纤维素材料; 和d)任选地,糖产品的回收; 其中在酶水解期间,将含氧气体加入到水解反应器中的木质纤维素材料中,并且其中添加到木质纤维素材料中的部分含氧气体是来自反应器的顶部空间的气体,优选在部分 酶水解的时间与木质纤维素材料的时间相比,与酶水解的其它部分相比较,或者优选在部分酶水解过程中,没有氧被添加到木质纤维素材料中。
    • 2. 发明申请
    • PROCESS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL AND FERMENTATION OF SUGARS
    • 木质纤维素材料的酶解水解和糖的发酵工艺
    • WO2014072390A1
    • 2014-05-15
    • PCT/EP2013/073250
    • 2013-11-07
    • DSM IP ASSETS B.V.
    • NOORDAM, Bertus
    • C12P19/14
    • C12P19/14C12P19/02Y02E50/16
    • The invention relates to a process for the preparation of a fermentation product from ligno-cellulosic material, comprising the following steps: a) optionally pre-treatment of the ligno-cellulosic material; b) optionally washing of the optionally pre-treated ligno-cellulosic material; c) enzymatic hydrolysis of the optionally washed and/or optionally pre-treated ligno-cellulosic material using an enzyme composition comprising at least two cellulase and whereby the enzyme composition at least comprises GH61; d)fermentation of the hydrolysed ligno-cellulosic material to produce a fermentation product; and e)optionally recovery of a fermentation product; wherein before and/or during the enzymatic hydrolysis oxygen is added to the ligno-cellulosic material.
    • 本发明涉及一种从木质纤维素材料制备发酵产物的方法,包括以下步骤:a)任选地预处理木素纤维素材料; b)任选地洗涤任选预处理的木质纤维素材料; c)使用包含至少两种纤维素酶的酶组合物对任选洗涤和/或任选预处理的木质纤维素材料进行酶水解,由此所述酶组合物至少包含GH61; d)水解的木质纤维素材料的发酵以产生发酵产物; 和e)任选地回收发酵产物; 其中在酶水解之前和/或期间,向木质纤维素材料中加入氧气。
    • 7. 发明申请
    • PROCESS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL
    • 木质纤维素材料的酶解水解方法
    • WO2014108454A1
    • 2014-07-17
    • PCT/EP2014/050271
    • 2014-01-09
    • DSM IP ASSETS B.V.
    • SMITS, Johannes PetrusBERKHOUT, Michael Petrus JozefNOORDAM, Bertus
    • C12P19/14C12P19/02C13K1/02
    • C12P19/02C12P19/14C12P2201/00C12Y302/01004C13K1/02
    • The invention relates to a process for the hydrolysis of cellulose containing biomass which comprises • a liquefaction step in which a first enzyme or first enzyme composition is added to liquefy at least part of the solids present in the biomass and to keep the viscosity of the cellulose containing biomass below 1000 cP, preferably below 800 cP, more preferably below 600 cP in the liquefaction step; followed by • a saccharification step in which a second enzyme composition is added to form oligomeric and/or monomeric sugars; and • whereby the first enzyme or first enzyme composition is different from the second enzyme composition; • whereby the first enzyme or first enzyme composition comprises an endoglucanase; ⋅ whereby the second enzyme composition comprises a cellulase; and • whereby the first enzyme or first enzyme composition comprises more endogluconase than the second enzyme composition (expressed in protein wt%).
    • 本发明涉及一种水解含纤维素生物质的方法,其包括:液化步骤,其中加入第一种酶或第一种酶组合物以液化存在于生物质中的至少部分固体,并保持纤维素的粘度 在液化步骤中含有低于1000cP的生物质,优选低于800cP,更优选低于600cP; 其次是糖化步骤,其中加入第二种酶组合物以形成低聚和/或单体糖; 和•由此第一酶或第一酶组合物不同于第二酶组合物; •由此第一酶或第一酶组合物包含内切葡聚糖酶; ⋅由此第二酶组合物包含纤维素酶; 和•由此第一酶或第一酶组合物包含比第二酶组合物更多的内切葡聚糖酶(以蛋白质wt%表示)。