基本信息:
- 专利标题: PROCESS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL
- 专利标题(中):木质纤维素材料的酶解水解方法
- 申请号:PCT/EP2014/050271 申请日:2014-01-09
- 公开(公告)号:WO2014108454A1 公开(公告)日:2014-07-17
- 发明人: SMITS, Johannes Petrus , BERKHOUT, Michael Petrus Jozef , NOORDAM, Bertus
- 申请人: DSM IP ASSETS B.V.
- 申请人地址: Het Overloon 1 NL-6411 TE Heerlen NL
- 专利权人: DSM IP ASSETS B.V.
- 当前专利权人: DSM IP ASSETS B.V.
- 当前专利权人地址: Het Overloon 1 NL-6411 TE Heerlen NL
- 代理机构: MATULEWICZ, Emil Rudolf Antonius
- 优先权: EP13150932.5 20130111
- 主分类号: C12P19/14
- IPC分类号: C12P19/14 ; C12P19/02 ; C13K1/02
摘要:
The invention relates to a process for the hydrolysis of cellulose containing biomass which comprises • a liquefaction step in which a first enzyme or first enzyme composition is added to liquefy at least part of the solids present in the biomass and to keep the viscosity of the cellulose containing biomass below 1000 cP, preferably below 800 cP, more preferably below 600 cP in the liquefaction step; followed by • a saccharification step in which a second enzyme composition is added to form oligomeric and/or monomeric sugars; and • whereby the first enzyme or first enzyme composition is different from the second enzyme composition; • whereby the first enzyme or first enzyme composition comprises an endoglucanase; ⋅ whereby the second enzyme composition comprises a cellulase; and • whereby the first enzyme or first enzyme composition comprises more endogluconase than the second enzyme composition (expressed in protein wt%).
摘要(中):
本发明涉及一种水解含纤维素生物质的方法,其包括:液化步骤,其中加入第一种酶或第一种酶组合物以液化存在于生物质中的至少部分固体,并保持纤维素的粘度 在液化步骤中含有低于1000cP的生物质,优选低于800cP,更优选低于600cP; 其次是糖化步骤,其中加入第二种酶组合物以形成低聚和/或单体糖; 和•由此第一酶或第一酶组合物不同于第二酶组合物; •由此第一酶或第一酶组合物包含内切葡聚糖酶; ⋅由此第二酶组合物包含纤维素酶; 和•由此第一酶或第一酶组合物包含比第二酶组合物更多的内切葡聚糖酶(以蛋白质wt%表示)。
IPC结构图谱:
C | 化学;冶金 |
--C12 | 生物化学;啤酒;烈性酒;果汁酒;醋;微生物学;酶学;突变或遗传工程 |
----C12P | 发酵或使用酶的方法合成目标化合物或组合物或从外消旋混合物中分离旋光异构体 |
------C12P19/00 | 含有糖残基的化合物的制备 |
--------C12P19/14 | .由碳水化合物酶作用产生的,如由α-淀粉酶 |