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1. 发明申请

WO2015021600A1 BETA-AMYLASE AND METHODS OF USE 审中-公开
标题翻译： β-淀粉酶和使用方法
公开(公告)号：WO2015021600A1
公开(公告)日：2015-02-19
申请号：PCT/CN2013/081383
申请日：2013-08-13
申请人： DANISCO US INC. , ADAMS, Christian D. , HUA, Ling , SHETTY, Jayarama K. , SONG, Danfeng , TANG, Zhongmei , XIE, Zhiyong , ZHANG, Bo , ZOU, Zhengzheng
发明人： ADAMS, Christian D. , HUA, Ling , SHETTY, Jayarama K. , SONG, Danfeng , TANG, Zhongmei , XIE, Zhiyong , ZHANG, Bo , ZOU, Zhengzheng
IPC分类号： C07K14/195 , C12N9/26 , C12N15/56
CPC分类号： C12N9/2425 , C12Y302/01002
摘要： The present teachings provide a beta amylase with maltogenic properties. Non-naturally occuring nucleic acids encoding the beta amylase and variants thereof, expression vectors, formulations, and host cells are also provided. Additional embodiments of the present teachings provide various methods of use and methods of manufacturing.
摘要翻译：本发明提供具有麦芽糖特性的β淀粉酶。还提供了编码β淀粉酶及其变体，表达载体，制剂和宿主细胞的非天然存在的核酸。本教导的另外的实施例提供了各种使用方法和制造方法。

2. 发明申请

WO2014204596A1 ALPHA-AMYLASE FROM BACILLACEAE FAMILY MEMBER 审中-公开
标题翻译：来自BACILLACEAE家族成员的ALPHA-淀粉酶
公开(公告)号：WO2014204596A1
公开(公告)日：2014-12-24
申请号：PCT/US2014/038112
申请日：2014-05-15
申请人： DANISCO US INC.
发明人： HUA, Ling , KOLKMAN, Marc , QIAN, Zhen , ZHANG, Bo
IPC分类号： C12N9/26 , A23L1/09 , C11D3/386
CPC分类号： C12N9/2417 , A23K20/189 , A23L29/35 , C11D3/386 , C11D3/38681 , C12N9/2414 , C12Y302/01001
摘要： Disclosed are compositions and methods relating to an alpha-amylase from a Bacillaceae family member. The compositions and methods are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing ( e.g. , desizing), in animal feed for improving digestibility, and for baking and brewing.
摘要翻译：公开了与来自芽孢杆菌科家族成员的α-淀粉酶相关的组合物和方法。组合物和方法可用于例如淀粉液化和糖化，用于清洁衣物，餐具洗涤和其它应用中的淀粉污渍，用于织物加工（例如脱浆），用于改善消化率的动物饲料中，以及烘烤和酿造。

3. 发明申请

WO2014093125A1 METHOD OF USING ALPHA-AMYLASE FROM ASPERGILLUS FUMIGATUS AND ISOAMYLASE FOR SACCHARIFICATION 审中-公开
标题翻译：从ASPERGILLUS FUMIGATUS和ISOAMYLASE使用ALPHA-AMYLASE进行鉴定的方法
公开(公告)号：WO2014093125A1
公开(公告)日：2014-06-19
申请号：PCT/US2013/073317
申请日：2013-12-05
申请人： DANISCO US INC.
发明人： HUA, Ling , SCHEFFERS, Martijn , VAN BRUSSEL, Marco , VROEMEN, Casper , ZHANG, Bo , ZHANG, Zhenghong
IPC分类号： C12P19/14 , C12N9/28 , C12P7/06 , C12C11/00 , A23L1/105 , A21D8/04 , C11D3/386 , D06M16/00
CPC分类号： C12P19/14 , A21D8/042 , A23L7/107 , A23V2002/00 , C11D3/386 , C12C5/004 , C12C11/003 , C12N9/242 , C12N9/246 , C12P7/06 , C12P19/02 , C12Y302/01001 , C12Y302/01068 , D06L1/06 , Y02E50/17
摘要： A fungal alpha-amylase is provided from Aspergillus fumigatus (AfAmyl). AfAmyl has an optimal pH of 3.5 and is operable at 30 - 75 degrees C, allowing the enzyme to be used in combination with a glucoamylase and an isoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase. AfAmyl also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DPI + DP2) compared to the products of saccharification catalyzed by an alpha-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.
摘要翻译：真菌α-淀粉酶由烟曲霉（AfAmyl）提供。 AfAmyl具有3.5的最佳pH，并且在30-75℃下可操作，允许酶在糖化反应中与葡糖淀粉酶和异淀粉酶组合使用。这消除了作为分批方法进行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。与由川崎曲霉α-淀粉酶催化的糖化产物相比，AfAmyl还催化淀粉底物对显着富集DP2和（DPI + DP2）的寡糖组合物的糖化。这有助于例如在同时糖化和发酵过程中由发酵生物体利用寡糖组合物。

4. 发明申请

WO2014099415A1 METHOD OF USING ALPHA-AMYLASE FROM ASPERGILLUS TERREUS AND PULLULANASE FOR SACCHARIFICATION 审中-公开
标题翻译：使用来自青蒿素和富勒烯的ALPHA-AMYLASE进行分离的方法
公开(公告)号：WO2014099415A1
公开(公告)日：2014-06-26
申请号：PCT/US2013/073594
申请日：2013-12-06
申请人： DANISCO US INC.
发明人： GE, Jing , HUA, Ling , SCHEFFERS, Martijn , VAN BRUSSEL, Marco , VROEMEN, Casper , ZHANG, Bo
IPC分类号： C12P19/14 , C12N9/24 , A21D8/04 , A23L1/10 , C11D3/386 , C12C11/00 , D06M16/00
CPC分类号： C12P19/14 , A21D8/042 , A23L2/382 , A23L7/107 , A23L29/35 , C11D3/386 , C12C5/004 , C12C7/04 , C12C7/16 , C12C11/003 , C12C12/04 , C12N9/242 , C12P7/06 , C12P7/14 , C12P19/02 , C12P2203/00 , C13K1/06 , C13K11/00 , D06L1/06 , Y02E50/17
摘要： A fungal alpha-amylase is provided from Aspergillus terreus (AtAmy1). AtAmy1 has an optimal pH of 4.5 and is operable at 30 75 degrees C, allowing the enzyme to be used in combination with a glucoamylase and a pullulanase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase. AtAmy1 also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1 + DP2) compared to the products of saccharification catalyzed by an alpha-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.
摘要翻译：从Aspergillus terreus（AtAmy1）提供真菌α-淀粉酶。 AtAmy1具有4.5的最佳pH，并且在30±75℃下可操作，使得酶可在糖化反应中与葡糖淀粉酶和支链淀粉酶组合使用。这消除了作为分批方法进行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。与由来自曲霉（Aspergillus kawachii）的α-淀粉酶催化的糖化产物相比，AtAmy1还催化淀粉底物对显着富含DP2和（DP1 + DP2）的寡糖组合物的糖化。这有助于例如在同时糖化和发酵过程中由发酵生物体利用寡糖组合物。

5. 发明申请

WO2014092960A1 TRICHODERMA REESEI HOST CELLS EXPRESSING A GLUCOAMYLASE FROM ASPERGILLUS FUMIGATUS AND METHODS OF USE THEREOF 审中-公开
标题翻译：表达来自青蒿素的谷氨酰胺酶的TRICHODERMA REESEI HOST细胞及其使用方法
公开(公告)号：WO2014092960A1
公开(公告)日：2014-06-19
申请号：PCT/US2013/071154
申请日：2013-11-21
申请人： DANISCO US INC.
发明人： GE, Jing , HUA, Ling , LEE, Sung Ho , LI, Jalsen , SHETTY, Jayarama, K. , TANG, Zhongmei , ZHANG, Bo , ZHONG, Kun
IPC分类号： C12N1/14 , C12N9/34 , C12N15/80 , C12P19/14
CPC分类号： C12P19/14 , C12N9/2428 , C12P19/02 , C12Y302/01003 , Y02E50/17
摘要： Fungal glucoamylases from Aspergillus fumigatus - expressed in Trichoderma reesei host cells (AfGATR) are provided. Trichoderma reesei host cells express AfGATRs at higher, or at least comparable, levels to natively expressed AfGA Aspergillus fumigatus . AfGATRs, including AfGA1TR and AfGA2TR, exhibit high activity at elevated temperatures and at low pH, so AfGATRs can be used efficiently in a process of saccharification in the presence of alpha-amylase, such as Aspergillus kawachii alpha-amylase (AkAA). AfGATRs advantageously catalyze starch saccharification to an oligosaccharide composition significantly enriched in DP1 (i.e., glucose) compared to the products of saccharification catalyzed by Aspergillus niger glucoamylase (AnGA) or native AfGA expressed in Aspergillus fumigatus . AfGATRs such as AfGA1TR, AfGA2TR or a variant thereof can be used at a lower dosage than AnGA and natively expressed AfGAs to produce comparable levels of glucose.
摘要翻译：提供来自烟曲霉的真菌葡糖淀粉酶 - 在里氏木霉宿主细胞（AfGATR）中表达。里氏木霉宿主细胞以更高或至少相当的水平将AfGATR表达为天然表达的AfGA烟曲霉（Aspergillus fumigatus）。包括AfGA1TR和AfGA2TR的AfGATR在升高的温度和低pH下表现出高活性，因此在α-淀粉酶例如曲霉曲霉α-淀粉酶（AkAA）的存在下，可以有效地使用AfGATR。与由烟曲霉中表达的黑曲霉葡糖淀粉酶（AnGA）或天然AfGA催化的糖化产物相比，AfGATR有利地将淀粉糖化催化剂显着富集DP1（即葡萄糖）的寡糖组合物。 AfGATR如AfGA1TR，AfGA2TR或其变体可以以比AnGA和天然表达的AfGA更低的剂量使用以产生相当水平的葡萄糖。

6. 发明申请

WO2014088935A3 COMPOSITIONS OF BETA-MANNANASE AND METHODS OF USE 审中-公开
公开(公告)号：WO2014088935A3
公开(公告)日：2014-06-12
申请号：PCT/US2013/072576
申请日：2013-12-02
申请人： DANISCO US INC.
发明人： HUA, Ling , LAU, Rosalyn , LE, Steven , QIAN, Zhen , YU, Zheyong
IPC分类号： C07K14/32 , C12N9/24
摘要： The present compositions and methods relate to a beta-mannanase from Bacillus licheniformis , polynucleotides encoding the beta-mannanase, and methods of make and/or use thereof. Formulations containing the beta-mannanase are suitable for use in hydrolyzing lignocellulosic biomass substrates, especially those comprising a measurable level of galactoglucomannan (GGM) and/or glucomannan (GM).

7. 发明申请

WO2014088934A1 COMPOSITIONS AND METHODS OF USE 审中-公开
标题翻译：组合物和使用方法
公开(公告)号：WO2014088934A1
公开(公告)日：2014-06-12
申请号：PCT/US2013/072572
申请日：2013-12-02
申请人： DANISCO US INC.
发明人： HUA, Ling , LAU, Rosalyn , LE, Steven , QIAN, Zhen , YU, Zheyong
IPC分类号： C12N9/24
CPC分类号： C12P19/14 , C12N9/2437 , C12N9/2494 , C12P19/02 , C12P2203/00 , C12Y302/01004 , C12Y302/01078
摘要： The present compositions and methods relate to a beta-mannanase from Paenibacillus kribbensis, polynucleotides encoding the beta-mannanase, and methods of make and/or use thereof. Formulations containing the beta-mannanase are suitable for use in hydrolyzing lignocellulosic biomass substrates, especially those comprising a measurable level of galactoglucomannan (GGM) and/or glucomannan (GM).
摘要翻译：本发明的组合物和方法涉及来自柯氏芽孢杆菌的β-甘露聚糖酶，编码β-甘露聚糖酶的多核苷酸，及其制备和/或使用方法。含有β-甘露聚糖酶的制剂适用于水解木质纤维素生物质底物，特别是含有可测量水平的半乳甘露聚糖（GGM）和/或葡甘露聚糖（GM）的那些。

8. 发明申请

WO2014200657A1 ALPHA-AMYLASE FROM STREPTOMYCES XIAMENENSIS 审中-公开
标题翻译： ALPHA-AMYLASE从STREPTOMYCES XIAMENENSIS
公开(公告)号：WO2014200657A1
公开(公告)日：2014-12-18
申请号：PCT/US2014/038134
申请日：2014-05-15
申请人： DANISCO US INC.
发明人： HUA, Ling , KOLKMAN, Marc , QIAN, Zhen , SONG, Danfeng , ZHANG, Bo
IPC分类号： C12N9/28 , C12P19/14 , C11D3/386 , A23L1/03
CPC分类号： C12N9/2417 , A21D8/042 , A23L7/107 , A23L29/06 , A23L29/35 , C11D3/38681 , C12P19/02 , C12P19/14 , C12Y302/01001 , D06L1/14 , D06L4/40
摘要： Disclosed are compositions and methods relating to an alpha-amylase from Streptomyces xiamenensis . The compositions and methods are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing.
摘要翻译：公开了与厦门蓝链霉菌的α-淀粉酶相关的组合物和方法。组合物和方法可用于例如淀粉液化和糖化，用于清洁衣物，餐具洗涤和其它应用中的淀粉污渍，用于织物加工（例如脱浆），用于改善消化率的动物饲料中，以及烘烤和酿造。

9. 发明申请

WO2014099416A1 METHOD OF USING ALPHA-AMYLASE FROM ASPERGILLUS TERREUS AND ISOAMYLASE FOR SACCHARIFICATION 审中-公开
标题翻译：从ASPERGILLUS TERREUS和ISOAMYLASE使用ALPHA-AMYLASE进行分类的方法
公开(公告)号：WO2014099416A1
公开(公告)日：2014-06-26
申请号：PCT/US2013/073601
申请日：2013-12-06
申请人： DANISCO US INC.
发明人： GE, Jing , HUA, Ling , SCHEFFERS, Martijn , VAN BRUSSEL, Marco , VROEMEN, Casper , ZHANG, Bo
IPC分类号： C12P19/14 , C12N9/30 , A21D8/04 , A23L1/10 , C11D3/386 , C12C11/00 , D06M16/00
CPC分类号： C12P19/14 , A21D8/042 , A23L2/382 , A23L7/107 , A23L29/35 , C11D3/38618 , C11D3/38681 , C12C5/004 , C12N9/242 , C12P7/14 , C12P19/02 , D06L1/14 , D06M16/003
摘要： A fungal alpha-amylase is provided from Aspergillus terreus (AtAmy1). AtAmy1 has an optimal pH of 4.5 and is operable at 30 75 degrees C, allowing the enzyme to be used in combination with a glucoamylase and an isoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase. AtAmy1 also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1 + DP2) compared to the products of saccharification catalyzed by an alpha amylase from Aspergillus kawachii . This facilitates the utilization of the ligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.
摘要翻译：从Aspergillus terreus（AtAmy1）提供真菌α-淀粉酶。 AtAmy1具有4.5的最佳pH，并且在30±75℃下可操作，使得酶可以在糖化反应中与葡糖淀粉酶和异淀粉酶组合使用。这消除了作为分批方法进行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。与由来自曲霉（Aspergillus kawachii）的α淀粉酶催化的糖化产物相比，AtAmy1还催化淀粉底物对显着富集DP2和（DP1 + DP2）的寡糖组合物的糖化。这有利于在同时糖化和发酵过程中由发酵生物体利用该链状糖组合物。

10. 发明申请

WO2014093123A1 METHOD OF USING ALPHA-AMYLASE FROM ASPERGILLUS FUMIGATUS AND PULLULANASE FOR SACCHARIFICATION 审中-公开
标题翻译：使用来自ASPERGILLUS FUMIGATUS和PULLULANASE的ALPHA-AMYLASE进行鉴定的方法
公开(公告)号：WO2014093123A1
公开(公告)日：2014-06-19
申请号：PCT/US2013/073304
申请日：2013-12-05
申请人： DANISCO US INC.
发明人： HUA, Ling , SCHEFFERS, Martijn , VAN BRUSSEL, Marco , VROEMEN, Casper , ZHANG, Bo , ZHANG, Zhenghong
IPC分类号： C12P19/14 , C12N9/30 , A21D8/04 , A23L1/10 , C11D3/386 , D06M16/00
CPC分类号： C12P19/02 , A21D8/042 , A23L2/382 , C11D3/38636 , C12C5/004 , C12C7/04 , C12C7/16 , C12C11/003 , C12C12/04 , C12G3/02 , C12N9/242 , C12P7/14 , C12P19/14 , C12Y302/01001 , C13K1/06 , D06L1/06
摘要： A fungal alpha-amylase is provided from Aspergillus fumigatus (AfAmy1). AfAmy1 has an optimal pH of 3.5 and is operable at 30 - 75 degrees C, allowing the enzyme to be used in combination with a glucoamylase and a pullulanase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase. AfAmy1 also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1 + DP2) compared to the products of saccharification catalyzed by an alpha-amylase from Aspergillus kawachii . This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.
摘要翻译：从烟曲霉（AfAmy1）提供真菌α-淀粉酶。 AfAmy1具有3.5的最佳pH，并且在30-75℃下可操作，使酶在糖化反应中与葡糖淀粉酶和支链淀粉酶组合使用。这消除了作为分批方法进行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。与由川曲霉α-淀粉酶催化的糖化产物相比，AfAmy1还催化淀粉底物对显着富含DP2和（DP1 + DP2）的寡糖组合物的糖化。这有助于例如在同时糖化和发酵过程中由发酵生物体利用寡糖组合物。

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