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    • 4. 发明申请
    • IDENTIFICATION OF NOVEL E2F TARGET GENES AND USE THEREOF
    • 鉴定E2F目标基因及其用途
    • WO2004035798A2
    • 2004-04-29
    • PCT/EP0311658
    • 2003-10-20
    • CROPDESIGN NVINZE DIRKDE VEYLDER LIEVENVLIEGHE KOBE
    • INZE DIRKDE VEYLDER LIEVENVLIEGHE KOBE
    • A01H1/04A01H5/00A61K31/713A61K38/16C07K14/415C12N15/82
    • C12N15/8261C07K14/415Y02A40/146
    • The present invention concerns a method for altering characteristics of a plant. The invention describes the identification of genes that are upregulated or downregulated in transgenic plants overexpressing E2Fa/DPa and the use of such sequences to alter plant characteristics. A preferred way for altering characteristics of a plant comprises modifying expression of one or more nucleic acid sequences and or modifying level and/or activity of one or more proteins, which nucleic acids and/or proteins are essentially similar to any one or more of SEQ ID NO 1 to 2755. Some of the genes identified in the present invention have an E2Fa target consensus sequence in their 5' upstream region. The identified genes play a role in a variety of biological processes, such as DNA replication, cell wall biosynthesis, nitrogen and/or carbon metabolism, transcription factors etc.
    • 本发明涉及改变植物特性的方法。 本发明描述了在过表达E2Fa / DPa的转基因植物中上调或下调的基因的鉴定,以及使用这种序列改变植物特征。 用于改变植物特征的优选方法包括修饰一个或多个核酸序列的表达和/或修饰一种或多种蛋白质的水平和/或活性,所述核酸和/或蛋白质基本上类似于SEQ ID NO: ID NO 1至2755.本发明中鉴定的一些基因在其5'上游区具有E2Fa靶共有序列。 鉴定的基因在多种生物过程中起作用,如DNA复制,细胞壁生物合成,氮和/或碳代谢,转录因子等。
    • 6. 发明申请
    • METHOD AND MEANS FOR MODULATING PLANT CELL CYCLE PROTEINS AND THEIR USE IN CONTROLLING PLANT CELL GROWTH
    • 调节植物细胞周期蛋白的方法和手段及其在控制植物细胞生长中的应用
    • WO9913083A9
    • 2001-03-29
    • PCT/EP9805673
    • 1998-09-07
    • CROPDESIGN NVINZE DIRKSEGERS GERDAVEYLDER LIEVEN DE
    • INZE DIRKSEGERS GERDADE VEYLDER LIEVEN
    • A01H5/00A01N61/00A01N65/08C07K14/415C07K16/16C12N1/15C12N1/19C12N1/21C12N5/10C12N15/09C12N15/29C12N15/82C12P21/02G01N33/15G01N33/50G01N33/53A01N65/00
    • A01N65/08A01N65/00C07K14/415C12N15/8261Y02A40/146A01N2300/00
    • Provided are DNA sequences encoding cell cycle interacting proteins as well as to methods for obtaining the same. Furthermore, vectors comprising said DNA sequences are described, wherein the DNA sequences are operatively linked to regulatory elements allowing expression in prokaryotic and/or eukaryotic host cells. In addition, proteins encoded by said DNA sequences, antibodies to said proteins and methods for their production are provided. Also described is a method for controlling or altering growth characteristics of a plant and/or a plant cell comprising introduction and/or expression of one or more cell cycle regulatory proteins functional in a plant or parts thereof and/or one or more DNA sequences encoding such proteins. Also provided is a process for disruption plant cell division by interfering in the expression of a substrate for cyclin-dependent protein kinase using a DNA sequence according to the invention wherein said plant cell is part of a transgenic plant. Further described are diagnostic compositions comprising the aforementioned DNA sequences, proteins and antibodies. Methods for the identification of compounds being capable of activating or inhibiting the cell cycle are described as well. Furthermore, transgenic plant cells, plant tissue and plants containing the above-described DNA sequences and vectors are described as well as the use of the aforementioned DNA sequences, vectors, proteins, antibodies and/or compounds identified by the method of the invention in plant cell and tissue culture, plant breeding and/or agriculture.
    • 提供编码细胞周期相互作用蛋白质的DNA序列以及获得该DNA序列的方法。 此外,描述了包含所述DNA序列的载体,其中所述DNA序列可操作地连接到允许在原核和/或真核宿主细胞中表达的调节元件。 此外,提供了由所述DNA序列编码的蛋白质,对所述蛋白质的抗体及其生产方法。 还描述了一种用于控制或改变植物和/或植物细胞的生长特性的方法,包括在植物或其部分中引入和/或表达一种或多种功能的细胞周期调节蛋白和/或编码 这样的蛋白质。 还提供了使用根据本发明的DNA序列干扰细胞周期蛋白依赖性蛋白激酶底物的表达来破坏植物细胞分裂的方法,其中所述植物细胞是转基因植物的一部分。 进一步描述包括上述DNA序列,蛋白质和抗体的诊断组合物。 还描述了用于鉴定能够激活或抑制细胞周期的化合物的方法。 此外,描述了含有上述DNA序列和载体的转基因植物细胞,植物组织和植物以及通过本发明的方法鉴定的上述DNA序列,载体,蛋白质,抗体和/或化合物在植物中的用途 细胞和组织培养,植物育种和/或农业。
    • 7. 发明申请
    • METHOD AND MEANS FOR MODULATING PLANT CELL CYCLE PROTEINS AND THEIR USE IN CONTROLLING PLANT CELL GROWTH
    • 调节植物细胞周期蛋白的方法和手段及其在控制植物细胞生长中的用途
    • WO9913083A2
    • 1999-03-18
    • PCT/EP9805673
    • 1998-09-07
    • CROPDESIGN NVINZE DIRKSEGERS GERDAVEYLDER LIEVEN DE
    • INZE DIRKSEGERS GERDADE VEYLDER LIEVEN
    • A01H5/00A01N61/00A01N65/08C07K14/415C07K16/16C12N1/15C12N1/19C12N1/21C12N5/10C12N15/09C12N15/29C12N15/82C12P21/02G01N33/15G01N33/50G01N33/53A01N65/00
    • A01N65/08A01N65/00C07K14/415C12N15/8261Y02A40/146A01N2300/00
    • Provided are DNA sequences encoding cell cycle interacting proteins as well as to methods for obtaining the same. Furthermore, vectors comprising said DNA sequences are described, wherein the DNA sequences are operatively linked to regulatory elements allowing expression in prokaryotic and/or eukaryotic host cells. In addition, proteins encoded by said DNA sequences, antibodies to said proteins and methods for their production are provided. Also described is a method for controlling or altering growth characteristics of a plant and/or a plant cell comprising introduction and/or expression of one or more cell cycle regulatory proteins functional in a plant or parts thereof and/or one or more DNA sequences encoding such proteins. Also provided is a process for disruption plant cell division by interfering in the expression of a substrate for cyclin-dependent protein kinase using a DNA sequence according to the invention wherein said plant cell is part of a transgenic plant. Further described are diagnostic compositions comprising the aforementioned DNA sequences, proteins and antibodies. Methods for the identification of compounds being capable of activating or inhibiting the cell cycle are described as well. Furthermore, transgenic plant cells, plant tissue and plants containing the above-described DNA sequences and vectors are described as well as the use of the aforementioned DNA sequences, vectors, proteins, antibodies and/or compounds identified by the method of the invention in plant cell and tissue culture, plant breeding and/or agriculture.
    • 提供了编码细胞周期相互作用蛋白的DNA序列以及获得其的方法。 此外,描述了包含所述DNA序列的载体,其中所述DNA序列可操作地连接至允许在原核和/或真核宿主细胞中表达的调节元件。 另外,提供了由所述DNA序列编码的蛋白质,所述蛋白质的抗体及其制备方法。 还描述了用于控制或改变植物和/或植物细胞的生长特征的方法,所述方法包括引入和/或表达在植物或其部分中起作用的一种或多种细胞周期调控蛋白和/或一种或多种编码 这种蛋白质。 还提供了通过使用根据本发明的DNA序列干扰细胞周期蛋白依赖性蛋白激酶底物表达来破坏植物细胞分裂的方法,其中所述植物细胞是转基因植物的一部分。 进一步描述了包含上述DNA序列,蛋白质和抗体的诊断组合物。 还描述了鉴定能够激活或抑制细胞周期的化合物的方法。 此外,描述了包含上述DNA序列和载体的转基因植物细胞,植物组织和植物以及通过本发明方法鉴定的上述DNA序列,载体,蛋白质,抗体和/或化合物在植物中的用途 细胞和组织培养,植物育种和/或农业。
    • 8. 发明申请
    • METHOD TO IDENTIFY HERBICIDES, FUNGICIDES OR PLANT GROWTH REGULATORS
    • 鉴定除草剂,杀真菌剂或植物生长调节剂的方法
    • WO0120020A3
    • 2001-09-27
    • PCT/EP0008797
    • 2000-09-08
    • CROPDESIGN NVBOUNAGA SAKINAINZE DIRKVEYLDER LIEVEN DEMIRONOV VLADIMIR
    • BOUNAGA SAKINAINZE DIRKDE VEYLDER LIEVENMIRONOV VLADIMIR
    • C07K14/415C12N15/81C12Q1/02C12Q1/18A01H5/00A01N65/00C07K14/39C12N15/29C12N15/82
    • C07K14/415C12N15/81C12Q1/025C12Q1/18
    • The present invention relates to a method for screening and identification of compounds or compositions useful as herbicides, growth regulators or fungicides involving at least the following steps of addition of the compound or composition to be screened or identified to a culture or culture area of a yeast strain transformed with and expressing one or more plant cell cycle control genes or mutants thereof (phytoyeast) as well as to a control yeast strain; and, determining the effect on the phenotype (growth and/or cell division and/or cell size/shape) of said phytoyeast compared to said control yeast. The invention further relates to a bilogical screening assay (high throughput system) comprising the use of said phytoyeast expressing plant cell cycle control proteins to identify novel compounds or compositions that affect control yeast or phytoyeast phenotype. The present invention also relates to methods for producing a pesticide, herbicide, plant growth regulator or fungicide, comprising the steps of (a) identifying a compound or composition as defined above or a derivative or homologue thereof, and, (b) mixing said compound, composition, derivative or homologue thereof with an acceptable carrier. The invention also relates to the use of said compounds or compositions for inhibiting or stimulating plant growth and/or for increasing crop yield and/or for preventive or curative protection of the plant against fungal infection.
    • 本发明涉及用于筛选和鉴定用作除草剂,生长调节剂或杀真菌剂的化合物或组合物的方法,该方法至少包括以下步骤:将要筛选或鉴定的化合物或组合物添加到酵母的培养或培养区域 用一种或多种植物细胞周期控制基因或其突变体(植物药)转化并表达的菌株以及对照酵母菌株; 并且与所述对照酵母相比,确定对所述植物淀粉的表型(生长和/或细胞分裂和/或细胞大小/形状)的影响。 本发明还涉及生物筛选测定(高通量系统),其包括使用表达植物树脂的植物细胞周期控制蛋白来鉴定影响对照酵母或植物性表型的新化合物或组合物。 本发明还涉及生产农药,除草剂,植物生长调节剂或杀真菌剂的方法,其包括以下步骤:(a)鉴定上述化合物或组合物或其衍生物或同系物,和(b)将所述化合物 ,组合物,衍生物或同系物与可接受的载体。 本发明还涉及所述化合物或组合物用于抑制或刺激植物生长和/或增加作物产量和/或预防或治疗保护植物免受真菌感染的用途。
    • 10. 发明申请
    • PLANT CELL CYCLE GENES AND USES THEREOF
    • 植物细胞周期基因及其用途
    • WO0036124A3
    • 2000-11-23
    • PCT/EP9910084
    • 1999-12-17
    • CROPDESIGN NVVEYLDER LIEVEN DEBOUDOLF VERONIQUE KATELIJNE CETORRES ACOSTA JUAN ANTONIOINZE DIRK
    • DE VEYLDER LIEVENBOUDOLF VERONIQUE KATELIJNE CETORRES ACOSTA JUAN ANTONIOINZE DIRK
    • A01H5/00A61K38/00A61K38/16A61K39/00C07K14/415C07K16/16C12N1/20C12N15/11C12N15/82C12Q1/68G01N33/50
    • C12N15/8229A61K38/00C07K14/415C12N15/8241
    • Provided are DNA sequences encoding cell cycle interacting proteins as well as methods for obtaining the same. Furthermore, vectors comprising said DNA sequences are described, wherein the DNA sequences preferably are operatively linked to regulatory elements allowing expression in prokaryotic and/or eukaryotic host cells. In addition, proteins encoded by said DNA sequences, antibodies to said proteins and methods for their production are provided. Also described is a method for controlling or altering growth characteristics of a plant and/or a plant cell comprising introduction and/or expression of one or more cell cycle regulatory proteins functional in a plant or parts thereof and/or one or more DNA sequences encoding such proteins. Also provided are regulatory sequences controling the expression of the above described cell cycle interacting proteins. Method for the identification of compounds being capable of activating or inhibiting the cell cycle are described as well. Furhter described are diagnostic compositions comprising the aforementioned DNA sequences, regulatory sequences, proteins, antibodies, inhibitors and activators. Furthermore, transgenic plant cells, plant tissue and plants containing the above-described DNA sequences and vectors are described as well as the use of the aforementioned DNA sequences, vectors, proteins, regulatory sequences, antibodies and/or compounds identified by the method of the invention in plant cell and tissue culture, plant breeding and/or agriculture.
    • 提供编码细胞周期相互作用蛋白质的DNA序列以及获得该DNA序列的方法。 此外,描述了包含所述DNA序列的载体,其中所述DNA序列优选可操作地连接到允许在原核和/或真核宿主细胞中表达的调节元件。 此外,提供了由所述DNA序列编码的蛋白质,对所述蛋白质的抗体及其生产方法。 还描述了一种用于控制或改变植物和/或植物细胞的生长特性的方法,包括在植物或其部分中引入和/或表达一种或多种功能的细胞周期调节蛋白和/或编码 这样的蛋白质。 还提供了控制上述细胞周期相互作用蛋白的表达的调节序列。 还描述了用于鉴定能够激活或抑制细胞周期的化合物的方法。 描述的是包含上述DNA序列,调节序列,蛋白质,抗体,抑制剂和激活剂的诊断组合物。 此外,描述了含有上述DNA序列和载体的转基因植物细胞,植物组织和植物以及上述DNA序列,载体,蛋白质,调节序列,抗体和/或通过 在植物细胞和组织培养,植物育种和/或农业中的发明。