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1. 发明申请

WO2014125515A2 METHOD FOR MAKING AN ARRAY OF MICRO-NEEDLES 审中-公开
标题翻译：制造微针阵列的方法
公开(公告)号：WO2014125515A2
公开(公告)日：2014-08-21
申请号：PCT/IT2014/000036
申请日：2014-02-11
申请人： CONSIGLIO NAZIONALE DELLE RICERCHE , FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIA
发明人： FERRARO, Pietro , COPPOLA, Sara , FINIZIO, Andrea , GRILLI, Simonetta , VESPINI, Veronica , NETTI, Paolo, Antonio , VECCHIONE, Raffaele , ESPOSITO, Eliana
IPC分类号： A61M37/00
CPC分类号： A61M37/0015 , A61M2037/0046 , A61M2037/0053 , B29C37/0053 , B29C59/025 , B29L2031/7544 , B81B2201/055 , B81C1/00111
摘要： There is described a method (1) for making an array of micro-needles (16), comprising the steps of: - depositing (3) a plurality of drops (11) of a liquid substance comprising a polymer on a surface of a starting substrate ( 10 ); - positioning (4) a pyroelectric substrate (12) at a certain distance (D) from the starting substrate (10) in such a way that the drops (11) deposited are positioned between said surface (9) of the starting substrate (10) and a surface (14) of the pyroelectric substrate (12); varying the temperature (5) of the pyroelectric substrate (12) or a part thereof to induce on said surface (14) of the pyroelectric substrate (12) a charge density such that starting from the drops deposited (11), under the effect of an electrodynamic force, respective cones (13) are formed having a tip facing towards the pyroelectric substrate (12); - determining (6) a consolidation of the cones (13), to form said micro-needles, preventing the tip of said cones (13) from contacting said surfaces (14) of the pyroelectric substrate (12).
摘要翻译：描述了一种用于制造微针阵列（16）的方法（1），包括以下步骤： - 在起动器的表面上沉积（3）包含聚合物的液体物质的多个液滴（11）基板（10）; - 以起始衬底（10）的一定距离（D）定位（4）热电衬底（12），使得沉积的液滴（11）位于起始衬底（10）的所述表面（9）之间）和热电基板（12）的表面（14）; 改变热电基底（12）或其一部分的温度（5）以在热电基底（12）的所述表面（14）上诱导电荷密度，使得从沉积的液滴（11）开始，在形成电动力，各个锥体（13）形成为具有朝向热电基板（12）的尖端; - 确定（6）所述锥体（13）的固结，以形成所述微针，防止所述锥体（13）的尖端接触所述热电基底（12）的所述表面（14）。

2. 发明申请

WO2005088276A1 METHOD AND APPARATUS FOR THE TWO-DIMENSIONAL MAPPING OF THE ELECTRO-OPTICAL COEFFICIENT 审中-公开
标题翻译：用于电光系统二维映射的方法和装置
公开(公告)号：WO2005088276A1
公开(公告)日：2005-09-22
申请号：PCT/IT2005/000135
申请日：2005-03-11
申请人： CONSIGLIO NAZIONALE DELLE RICERCHE , DE ANGELIS, Marella , FERRARO, Pietro , FINIZIO, Andrea , GRILLI, Simonetta , DE NATALE, Paolo , DE NICOLA, Sergio , PIERATTINI, Giovanni
发明人： DE ANGELIS, Marella , FERRARO, Pietro , FINIZIO, Andrea , GRILLI, Simonetta , DE NATALE, Paolo , DE NICOLA, Sergio , PIERATTINI, Giovanni
IPC分类号： G01N21/45
CPC分类号： G01N21/453 , G03H1/041 , G03H1/0443 , G03H2210/12 , G03H2210/63
摘要： The invention concerns a method for measuring and two-dimensional mapping of the electro-optical properties of materials transparent to the electromagnetic radiation, comprising a first step A of reproducing of a substantially coherent and monochromatic electromagnetic beam, a subsequent step B of projection of the beam onto the transparent material to be analysed which is subjected to an electrical field, a subsequent step C of processing of said beam by means of an interferometric system (5) and a subsequent step D of detection of the beam exiting from said interferometric system characterised in that the projection of step B is realised in such a way that the beam is enlarged as far as to have a two-dimensional cross section comparable with the realised by means of device (7) suited to two-dimensionally detect the beam exiting from the interferometric system (5). The invention also concerns a cell to be utilised in the method according to the invention for applying an electrical field to the material, the cell using liquid electrodes, and an apparatus implementing such method.
摘要翻译：本发明涉及一种用于测量和对电磁辐射透明的材料的电光学性质的二维映射的方法，包括再现基本相干和单色电磁束的第一步骤A，后续步骤B的投影光束到经受电场的要分析的透明材料上，随后的通过干涉系统（5）处理所述光束的步骤C和随后的从所述干涉系统退出的光束的检测步骤D，其特征在于因为步骤B的投影以这样一种方式实现，使得光束被扩大到具有与通过适于二维地检测从干涉仪系统（5）。本发明还涉及在根据本发明的方法中用于向材料施加电场，使用液体电极的电池以及实施这种方法的装置中使用的电池。

3. 发明申请

WO2005017630A1 METHOD FOR MODIFYING SPATIAL RESOLUTION IN THE RECONSTRUCTION OF IMAGES IN DIGITAL HOLOGRAPHY 审中-公开
标题翻译：修改数字图像中图像重建的空间分辨率的方法
公开(公告)号：WO2005017630A1
公开(公告)日：2005-02-24
申请号：PCT/IT2004/000380
申请日：2004-07-09
申请人： CONSIGLIO NAZIONALE DELLE RICERCHE , COPPOLA, Giuseppe , DE NICOLA, Sergio , FERRARO, Pietro , FINIZIO, Andrea , PIERATTINI, Giovanni
发明人： COPPOLA, Giuseppe , DE NICOLA, Sergio , FERRARO, Pietro , FINIZIO, Andrea , PIERATTINI, Giovanni
IPC分类号： G03H1/08
CPC分类号： G03H1/0841 , G03H1/0443 , G03H1/0866 , G03H2240/62
摘要： The invention concerns a method for the reconstruction of holographic images in digital holography, the holographic images being detected and transformed in a digitized hologram by an image detection device, the digitized hologram being made of an array having a number Vr of elementary pixels, the sizes of which being equal to the sampling intervals of the image detection device, the method comprising a first step of processing the digitized hologram array (e.g. zero-order elimination, aberration correction), and a second step of digitally reconstructing the complex object in an observation plane starting from the digitized hologram processed in the first step, the method being characterised in that the second step is carried out through discrete Fresnel Transform applied on an array of Ve pixels having sizes equal to that of said elementary pixels, wherein said array of Ve pixels (50, 51) includes said Vr pixels (51) and an interger number p=Ve-Vr>0 constant values (50). The invention further concerns the instruments necessary to the execution of the method and the apparatus executing it.
摘要翻译：本发明涉及一种用于重建数字全息术全息图像的方法，通过图像检测装置检测和变换数字化全息图中的全息图像，数字化全息图由基本像素数Vr的阵列组成，尺寸其等于图像检测装置的采样间隔，该方法包括处理数字化全息图阵列的第一步骤（例如零阶消除，像差校正）以及在观察中数字重建复杂物体的第二步骤从第一步骤处理的数字化全息图开始，该方法的特征在于，第二步骤通过施加在尺寸等于所述基本像素的Ve像素阵列上的离散菲涅耳变换来执行，其中所述Ve 像素（50,51）包括所述Vr像素（51）和片间数p = Ve-Vr> 0常数值（50）。本发明还涉及执行该方法和执行该方法的设备所必需的仪器。

4. 发明申请

WO2015075698A1 METHOD AND APPARATUS FOR DISCRIMINATING X AND Y SPERMS BASED UPON RAMAN SPECTROSCOPY 审中-公开
标题翻译：基于拉曼光谱分析X和Y轴的方法和装置
公开(公告)号：WO2015075698A1
公开(公告)日：2015-05-28
申请号：PCT/IB2014/066319
申请日：2014-11-25
申请人： CONSIGLIO NAZIONALE DELLE RICERCHE
发明人： DE LUCA, Anna Chiara , BALDUZZI, Donatella , COPPOLA, Giuseppe , GALLI, Andrea , SIRLETO, Luigi , PUGLISI, Roberto , FERRARA, Maria Antonietta , FERRARO, Pietro
IPC分类号： G01N15/14 , C12N5/071 , G21K1/00 , G01N21/65 , G02B21/32
CPC分类号： G01N15/1459 , C12N5/0612 , G01N15/1484 , G01N21/65 , G01N2015/149 , G02B21/32
摘要： The invention relates to a method based upon the use of Raman spectroscopy to face the problem of the biochemical analysis and of the differentiation of the X sperm and Y sperm in the animal species, in particular bovine species, with approaches which make possible the subsequent use in fecundation techniques. A method addressed to the resolution of this problem is based upon the separation between X sperm and Y sperm before insemination. An apparatus is also described implementing such method which allows to distinguish and characterize the difference between X sperm and Y sperm by acquiring and analyzing Raman spectra noninvasively and in a not destructive way. By means of a statistical approach, such as for example the analysis of the main components and the linear discriminating analysis, the spectroscopic difference between X sperm and Y sperm is evaluated, processed and used to separate the two types of cells. The invention also relates to the development of a cytofluorimeter combining the Raman microscope with a system for trapping optically the sperms and a microfluidic chamber. More precisely, the optical trapping system, equipped with an objective with high numerical aperture, is used to immobilize the cells and, the same objective is used to excite and gather Raman spectra. At last, the microfluidic chamber is used to make the sample to flow, with the final purpose of providing a quick, precise and not invasive separation of X sperm and Y sperm, (figure 5A).
摘要翻译：本发明涉及一种基于使用拉曼光谱法来面对动物物种，特别是牛物种中的X精子和Y精子的生物化学分析和分化问题的方法，所述方法使随后的使用成为可能在fecundation技术。解决这个问题的方法是基于X精子和Y精子在授精前的分离。还描述了一种实现这种方法的装置，其通过以非侵入性和非破坏性的方式获取和分析拉曼光谱来区分和表征X精子和Y精子之间的差异。通过统计方法，例如主要成分的分析和线性鉴别分析，X精子与Y精子之间的光谱差异被评估，处理并用于分离两种细胞。本发明还涉及将拉曼显微镜与用于光学捕获精子和微流体室的系统结合的细胞荧光计的开发。更准确地说，配备有具有高数值孔径的物镜的光学捕获系统用于固定电池，并且使用相同的目的来激发和聚集拉曼光谱。最后，微流体室用于使样品流动，其最终目的是提供X精子和Y精子的快速，精确和不侵入的分离（图5A）。

5. 发明申请

WO2010150296A1 METHOD FOR CONTROLLING DEPTH-OF-FOCUS IN 3D IMAGE RECONSTRUCTIONS, IN PARTICULAR FOR SYNTHESIZING THREE-DIMENSIONAL DYNAMIC SCENE FOR THREE- DIMENSIONAL HOLOGRAPHY DISPLAY, AND HOLOGRAPHIC APPARATUS UTILIZING SUCH A METHOD 审中-公开
标题翻译：用于控制3D图像重建中的深度的方法，特别是用于合成三维全息显示的三维动态场景，以及使用这种方法的全景照相装置
公开(公告)号：WO2010150296A1
公开(公告)日：2010-12-29
申请号：PCT/IT2010/000281
申请日：2010-06-25
申请人： CONSIGLIO NAZIONALE DELLE RICERCHE , FERRARO, Pietro , PATURZO, Melania , MEMMOLO, Pasquale , FINIZIO, Andrea
发明人： FERRARO, Pietro , PATURZO, Melania , MEMMOLO, Pasquale , FINIZIO, Andrea
IPC分类号： G03H1/08 , G03H1/22
CPC分类号： G03H1/0866 , G03H1/2249 , G03H1/2294 , G03H2001/0088 , G03H2001/043 , G03H2001/0445 , G03H2001/0473 , G03H2001/0825 , G03H2001/0883 , G03H2001/2255 , G03H2001/2271 , G03H2001/2284 , G03H2210/20 , G03H2210/30 , G03H2210/62 , G03H2222/13 , G03H2240/52
摘要： The invention concerns a method for controlling depth or depth-of-focus in 3D image reconstructions, in particular for: A) Controlling the depth with the aim to synthesize a holography dynamic 3D scene; such a scene can be either numerically reconstructed or holographically projected for 3D display purposes by means of optical reconstruction through Spatial Light Modulator (SLM); B) controlling the focus to extend the depth of focus and have two object at different distance simultaneously in focus by digital holography; The method according to the invention can be applied with few differences both to the usual holograms and to the Fourier ones. The invention further concerns a holographic apparatus implementing the method according to the invention.
摘要翻译：本发明涉及一种用于控制3D图像重建中的深度或深度聚焦的方法，特别是用于：A）控制深度以便合成全息动态3D场景; 通过空间光调制器（SLM）的光学重建，这样的场景可以被数字重构或全息投影用于3D显示目的; B）控制焦点以扩大焦点的深度，并通过数字全息术同时聚焦于不同距离的两个物体; 根据本发明的方法可以应用于通常的全息图和傅里叶变换的方法。本发明还涉及实现根据本发明的方法的全息装置。

6. 发明申请

WO2007122655A2 QUANTITATIVE PHASE-CONTRAST DIGITAL HOLOGRAPHY METHOD FOR THE NUMERICAL RECONSTRUCTION OF IMAGES, AND RELEVANT APPARATUS 审中-公开
标题翻译：用于数字重建图像的量化相位数字全息方法及相关装置
公开(公告)号：WO2007122655A2
公开(公告)日：2007-11-01
申请号：PCT/IT2007/000224
申请日：2007-03-26
申请人： CONSIGLIO NAZIONALE DELLE RICERCHE , FERRARO, Pietro , ALFIERI, Domenico , DE NICOLA, Sergio , DE PETROCELLIS, Luciano , FINIZIO, Andrea , PIERATTINI, Giovanni
发明人： FERRARO, Pietro , ALFIERI, Domenico , DE NICOLA, Sergio , DE PETROCELLIS, Luciano , FINIZIO, Andrea , PIERATTINI, Giovanni
IPC分类号： G03H1/08
CPC分类号： G03H1/0866 , G03H1/0808 , G03H2001/0445 , G03H2001/0883
摘要： The invention concerns a quantitative phase-contrast digital holography method for the numerical reconstruction of images, comprising the following steps: A. acquiring a digital hologram of an investigated object; B. reconstructing the digital hologram in a recostruction plane; C. reconstructing the complex field for the digital hologram; D. obtaining the phase map starting from the complex field; the method being characterised in that it further comprises the following steps: E. applying to the digital matrix of any step A, B, C a shear s x and/or s y respectively along directions x and/or y ; F. subtracting the matrix obtained in step E from the starting matrix of step E, or vice versa; G. integrating the obtained matrix along directions x and/or y ; H. calculating at least a defocus aberration term; I. subtracting said at least a term calculated in step H from the matrix obtained in step G, the steps G to I being subsequent to step D. The invention further concerns a digital holography apparatus which implements the method of the invention.
摘要翻译：本发明涉及一种用于数字重建图像的定量相位对比数字全息方法，包括以下步骤：A.获取被检查物体的数字全息图; B.重建平面上的数字全息图; C.重建数字全息图的复杂场; D.从复场开始获得相位图; 该方法的特征在于它还包括以下步骤：E.应用任何步骤A，B，C的数字矩阵剪切

7. 发明申请

WO2023002355A1 COMPUTER-IMPLEMENTED METHOD AND CORRESPONDING APPARATUS FOR IDENTIFYING SUBCELLULAR STRUCTURES IN THE NON-CHEMICAL STAINING MODE FROM PHASE CONTRAST TOMOGRAPHY RECONSTRUCTIONS IN FLOW CYTOMETRY 审中-公开
公开(公告)号：WO2023002355A1
公开(公告)日：2023-01-26
申请号：PCT/IB2022/056625
申请日：2022-07-19
申请人： CONSIGLIO NAZIONALE DELLE RICERCHE
发明人： FERRARO, Pietro , PIRONE, Daniele , MEMMOLO, Pasquale , MICCIO, Lisa , BIANCO, Vittorio , PSALTIS, Demetri , LIM, Joowon
IPC分类号： G06V20/69 , G06K9/62
摘要： The present invention relates to a computer-implemented method and corresponding apparatus for the accurate identification of subcellular structures from tomographic reconstructions. This invention allows to extract the 3D subcellular specificity directly from the tomographic phase-contrast data obtained from a typical flow cytometry configuration or any other experimental tomographic systems able to provide 3D quantitative phase-contrast tomograms. In particular, subcellular structures can be identified by using a novel computational segmentation method based on statistical inference from any 3D phase-contrast tomographic data.

8. 发明申请

WO2010122592A1 DEVICE FOR CONTROLLED DISTRIBUTION OF MICRO- OR NANO- VOLUMES OF A LIQUID BASED ON THE PIEZOELECTRIC EFFECT IN FUNCTIONALISED MATERIALS, WITHOUT USING EXTERNAL ELECTRIC SOURCES 审中-公开
标题翻译：用于在不使用外部电源的情况下基于功能材料的压电效应控制分布液体的微波或纳米的装置
公开(公告)号：WO2010122592A1
公开(公告)日：2010-10-28
申请号：PCT/IT2010/000172
申请日：2010-04-21
申请人： CONSIGLIO NAZIONALE DELLE RICERCHE , FERRARO, Pietro , COPPOLA, Sara , VESPINI, Veronica , GRILLI, Simonetta , PATURZO, Melania
发明人： FERRARO, Pietro , COPPOLA, Sara , VESPINI, Veronica , GRILLI, Simonetta , PATURZO, Melania
IPC分类号： B01L3/00 , B05B5/025 , B05B17/04 , B05D1/02 , B41J2/005 , B81B1/00
CPC分类号： B01L3/0262 , B01J2219/00378 , B01L3/0268 , B01L2300/0819 , B01L2400/0415 , B01L2400/0448 , B41J2/005
摘要： The invention concerns a method for the controlled distribution of pico- or nano-volumes of a liquid, comprising the step of: A. Deposing the liquid as a film or separated sessile drops on a surface of a starting substrate; the liquid being extracted and distributed on the surface of a substrate called "destination substrate", the method being characterized in that: - At least one between said starting substrate and destination substrate is a pyroelectric substrate; The method comprising the following further step: B. Varying the temperature of said pyroelectric substrate to induce a surface charge density σ such that the liquid, undergoing the electro-dynamic force, creates dispensing cones wherefrom droplets detach, which are deposed on the surface of the destination substrate, The surface of the destination substrate being set at a distance D, from the surface of the starting substrate facing it, smaller than the critical distance given by the following: Dc = (1+θ/4)V 1/3 wherein 6Ms the contact angle of the dispensing cone and ^ the volume of the liquid of the same dispensing cone. The invention further concerns the relevant device for the controlled distribution of pico- or nanovolumes of liquid.
摘要翻译：本发明涉及一种用于液体的微米或纳米体积的受控分布的方法，包括以下步骤：A.将起始底物表面上的液体取代为薄膜或分离的无柄滴剂; 所述液体被提取并分布在称为“目的基板”的基板的表面上，其特征在于： - 所述起始基板和目的基板之间的至少一个为热电基板; 该方法包括以下进一步的步骤：B.改变所述热释电基材的温度以引起表面电荷密度，使得经受电动力的液体产生分散锥体，其中液滴脱离，其被放置在目的基板，目标基板的表面与起始基板的面对面的距离D设定为小于由下列给出的临界距离：Dc =（1 +π/ 4）V 1/3 其中6Ms是分配锥体的接触角和相同分配锥体的液体的体积。本发明还涉及用于液体的微波或纳米体积的受控分布的相关装置。

9. 发明申请

WO2007138632A1 METHOD FOR LASER-FREQUENCY LOCKING TO A FIBRE RESONATOR WITH INDUCE BIREFRINGENCE, METHOD OF INTERROGATION OF A STATIC AND/OR DYNAMIC STRAIN SENSOR MAKING USE OF SUCH A LOCKING METHOD, AND RELEVANT APPARATUSES 审中-公开
标题翻译：用于激光频率锁定到具有诱发双极性的光纤谐振器的方法，静态和/或动态应变传感器的互连方法制造这种锁定方法的使用以及相关装置
公开(公告)号：WO2007138632A1
公开(公告)日：2007-12-06
申请号：PCT/IT2007/000365
申请日：2007-05-25
申请人： CONSIGLIO NAZIONALE DELLE RICERCHE , GAGLIARDI, Gianluca , FERRARO, Pietro , DE NATALE, Paolo , DE NICOLA, Sergio
发明人： GAGLIARDI, Gianluca , FERRARO, Pietro , DE NATALE, Paolo , DE NICOLA, Sergio
IPC分类号： G01D5/353 , G01L1/24 , H01S3/13 , H01S5/0687
CPC分类号： G01D5/35303 , G01L1/246 , H01S3/1392 , H01S5/0687 , H01S5/12
摘要： The present invention concerns a method for laser-frequency locking to a fibre resonator with induced birefringence, comprising the use of a laser external to the fibre optical resonator with induced birefringence (RisF), characterised in that the reflected radiation by the fibre optical resonator with induced birefringence (RisF) is detected by means of a polarisation analyser (AP) and is used as reference signal for generating a correction signal which effectuates a feedback control of the laser. The invention further concerns a laser frequency-stabiliser apparatus, characterised in that it comprises a laser source, an optical fibre resonator with induced birefringence (RisF) and a fibre beam separator (FC) for the separation of the radiation incident in the cavity of the resonator from the reflected radiation, the reflected radiation being analysed by a polarisation analyser (AP) and then detected by at least a photodiode (PD-i, PD2), the detected radiation being used to obtain a correction signal that does a feedback control of the laser, so as to stabilise it. The invention also concerns a Method of interrogation of a sensor constituted by a fibre optical resonator with induced birefringence (RisF), characterised in that it uses a laser according to the above method of frequency locking according to the invention, and in that the correction signal which effectuates the feedback control of the laser is the monitoring signal of the state of the sensor (RisF). Finally the invention concerns an apparatus for the detection of static and dynamic strains, possibly due to temperature, comprising the components of the above stabilisation apparatus according to the invention, the optical resonator with induced birefringence (RisF) being used as sensor and the correction signal being detected and used as monitoring signal of the sensor.
摘要翻译：本发明涉及一种用于激光频率锁定到具有诱导双折射的光纤谐振器的方法，其包括使用具有诱导双折射（RisF）的光纤谐振器外部的激光器，其特征在于由光纤谐振器的反射辐射通过偏振分析器（AP）检测感应双折射（RisF），并将其用作用于产生校正信号的参考信号，该校正信号实现激光的反馈控制。本发明还涉及一种激光频率稳定器装置，其特征在于它包括激光源，具有诱导双折射（RisF）的光纤谐振器和用于分离入射在腔中的辐射的光纤分束器（FC）谐振器，反射辐射由偏振分析器（AP）分析，然后由至少一个光电二极管（PD-i，PD2）检测，所检测的辐射被用于获得执行反馈控制的校正信号激光，以稳定它。本发明还涉及一种由具有诱导双折射（RisF）的光纤谐振器构成的传感器的询问方法，其特征在于，使用根据本发明的上述频率锁定方法的激光器，并且所述校正信号这实现了激光器的反馈控制是传感器（RisF）状态的监控信号。最后，本发明涉及一种用于检测可能由于温度而导致的静态和动态应变的装置，其包括根据本发明的上述稳定装置的部件，将具有诱导双折射（RisF）的光学谐振器用作传感器和校正信号被检测并用作传感器的监控信号。

10. 发明申请

WO2006097956A3 HOLOGRAPHIC METHOD WITH NUMERICAL RECONSTRUCTION FOR OBTAINING AN IMAGE OF A THREE-DIMENSIONAL OBJECT IN WHICH EVEN POINTS OUT OF THE DEPTH OF FIELD ARE IN FOCUS, AND HOLOGRAPHIC APPARATUS USING SUCH A METHOD 审中-公开
公开(公告)号：WO2006097956A3
公开(公告)日：2006-09-21
申请号：PCT/IT2006/000099
申请日：2006-02-23
申请人： CONSIGLIO NAZIONALE DELLE RICERCHE , COPPOLA, Giuseppe , DE NICOLA, Sergio , FERRARO, Pietro , FINIZIO, Andrea , GRILLI, Simonetta , JAVIDI, Bahram , PIERATTINI, Giovanni
发明人： COPPOLA, Giuseppe , DE NICOLA, Sergio , FERRARO, Pietro , FINIZIO, Andrea , GRILLI, Simonetta , JAVIDI, Bahram , PIERATTINI, Giovanni
IPC分类号： G06T7/00 , G03H1/08
摘要： The invention concerns a holographic method with numerical reconstruction for obtaining an image of a three-dimensional object, said method employing a digitalised hologram of an object, and comprising a step A. wherein, starting from the digitalised hologram, extracting a phase image of said object corresponding to a bidimensional matrix MD of distance values; a step B. wherein a monodimensional subassembly SD of the distance value assembly present in matrix MD of the method step A is selected, subassembly SD containing distance values dk; a step C. wherein for each distance value dk, extracting from matrix MD a iso-level assembly IQdk corresponding to a monodimensional assembly of bidimensional coordinates of said object,- a step D. wherein for each distance value dk, reconstructing, starting from the digitalised hologram, a bidimensional matrix IMdk of intensity values relevant to said object; a step E. wherein, from each bidimensional matrix IMdk a bidimensional matrix IFdk of intensity values is extracted corresponding to the bidimensional coordinates of the iso-level assembly IQdk; a step F. wherein, starting from intensity values IFdk from the bidimensional coordinates of the iso-level assembly IQdk and from the relevant distance values dk, reconstructing the three-dimensional intensity image of said object, and wherein the resolution of the bidimensional matrix IMdk for all values of k is identical to the resolution of matrix MD of distance values.

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