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1. 发明申请

WO2005017630A1 METHOD FOR MODIFYING SPATIAL RESOLUTION IN THE RECONSTRUCTION OF IMAGES IN DIGITAL HOLOGRAPHY 审中-公开
标题翻译：修改数字图像中图像重建的空间分辨率的方法
公开(公告)号：WO2005017630A1
公开(公告)日：2005-02-24
申请号：PCT/IT2004/000380
申请日：2004-07-09
申请人： CONSIGLIO NAZIONALE DELLE RICERCHE , COPPOLA, Giuseppe , DE NICOLA, Sergio , FERRARO, Pietro , FINIZIO, Andrea , PIERATTINI, Giovanni
发明人： COPPOLA, Giuseppe , DE NICOLA, Sergio , FERRARO, Pietro , FINIZIO, Andrea , PIERATTINI, Giovanni
IPC分类号： G03H1/08
CPC分类号： G03H1/0841 , G03H1/0443 , G03H1/0866 , G03H2240/62
摘要： The invention concerns a method for the reconstruction of holographic images in digital holography, the holographic images being detected and transformed in a digitized hologram by an image detection device, the digitized hologram being made of an array having a number Vr of elementary pixels, the sizes of which being equal to the sampling intervals of the image detection device, the method comprising a first step of processing the digitized hologram array (e.g. zero-order elimination, aberration correction), and a second step of digitally reconstructing the complex object in an observation plane starting from the digitized hologram processed in the first step, the method being characterised in that the second step is carried out through discrete Fresnel Transform applied on an array of Ve pixels having sizes equal to that of said elementary pixels, wherein said array of Ve pixels (50, 51) includes said Vr pixels (51) and an interger number p=Ve-Vr>0 constant values (50). The invention further concerns the instruments necessary to the execution of the method and the apparatus executing it.
摘要翻译：本发明涉及一种用于重建数字全息术全息图像的方法，通过图像检测装置检测和变换数字化全息图中的全息图像，数字化全息图由基本像素数Vr的阵列组成，尺寸其等于图像检测装置的采样间隔，该方法包括处理数字化全息图阵列的第一步骤（例如零阶消除，像差校正）以及在观察中数字重建复杂物体的第二步骤从第一步骤处理的数字化全息图开始，该方法的特征在于，第二步骤通过施加在尺寸等于所述基本像素的Ve像素阵列上的离散菲涅耳变换来执行，其中所述Ve 像素（50,51）包括所述Vr像素（51）和片间数p = Ve-Vr> 0常数值（50）。本发明还涉及执行该方法和执行该方法的设备所必需的仪器。

2. 发明申请

WO2006097956A3 HOLOGRAPHIC METHOD WITH NUMERICAL RECONSTRUCTION FOR OBTAINING AN IMAGE OF A THREE-DIMENSIONAL OBJECT IN WHICH EVEN POINTS OUT OF THE DEPTH OF FIELD ARE IN FOCUS, AND HOLOGRAPHIC APPARATUS USING SUCH A METHOD 审中-公开
公开(公告)号：WO2006097956A3
公开(公告)日：2006-09-21
申请号：PCT/IT2006/000099
申请日：2006-02-23
申请人： CONSIGLIO NAZIONALE DELLE RICERCHE , COPPOLA, Giuseppe , DE NICOLA, Sergio , FERRARO, Pietro , FINIZIO, Andrea , GRILLI, Simonetta , JAVIDI, Bahram , PIERATTINI, Giovanni
发明人： COPPOLA, Giuseppe , DE NICOLA, Sergio , FERRARO, Pietro , FINIZIO, Andrea , GRILLI, Simonetta , JAVIDI, Bahram , PIERATTINI, Giovanni
IPC分类号： G06T7/00 , G03H1/08
摘要： The invention concerns a holographic method with numerical reconstruction for obtaining an image of a three-dimensional object, said method employing a digitalised hologram of an object, and comprising a step A. wherein, starting from the digitalised hologram, extracting a phase image of said object corresponding to a bidimensional matrix MD of distance values; a step B. wherein a monodimensional subassembly SD of the distance value assembly present in matrix MD of the method step A is selected, subassembly SD containing distance values dk; a step C. wherein for each distance value dk, extracting from matrix MD a iso-level assembly IQdk corresponding to a monodimensional assembly of bidimensional coordinates of said object,- a step D. wherein for each distance value dk, reconstructing, starting from the digitalised hologram, a bidimensional matrix IMdk of intensity values relevant to said object; a step E. wherein, from each bidimensional matrix IMdk a bidimensional matrix IFdk of intensity values is extracted corresponding to the bidimensional coordinates of the iso-level assembly IQdk; a step F. wherein, starting from intensity values IFdk from the bidimensional coordinates of the iso-level assembly IQdk and from the relevant distance values dk, reconstructing the three-dimensional intensity image of said object, and wherein the resolution of the bidimensional matrix IMdk for all values of k is identical to the resolution of matrix MD of distance values.

3. 发明申请

WO2015075698A1 METHOD AND APPARATUS FOR DISCRIMINATING X AND Y SPERMS BASED UPON RAMAN SPECTROSCOPY 审中-公开
标题翻译：基于拉曼光谱分析X和Y轴的方法和装置
公开(公告)号：WO2015075698A1
公开(公告)日：2015-05-28
申请号：PCT/IB2014/066319
申请日：2014-11-25
申请人： CONSIGLIO NAZIONALE DELLE RICERCHE
发明人： DE LUCA, Anna Chiara , BALDUZZI, Donatella , COPPOLA, Giuseppe , GALLI, Andrea , SIRLETO, Luigi , PUGLISI, Roberto , FERRARA, Maria Antonietta , FERRARO, Pietro
IPC分类号： G01N15/14 , C12N5/071 , G21K1/00 , G01N21/65 , G02B21/32
CPC分类号： G01N15/1459 , C12N5/0612 , G01N15/1484 , G01N21/65 , G01N2015/149 , G02B21/32
摘要： The invention relates to a method based upon the use of Raman spectroscopy to face the problem of the biochemical analysis and of the differentiation of the X sperm and Y sperm in the animal species, in particular bovine species, with approaches which make possible the subsequent use in fecundation techniques. A method addressed to the resolution of this problem is based upon the separation between X sperm and Y sperm before insemination. An apparatus is also described implementing such method which allows to distinguish and characterize the difference between X sperm and Y sperm by acquiring and analyzing Raman spectra noninvasively and in a not destructive way. By means of a statistical approach, such as for example the analysis of the main components and the linear discriminating analysis, the spectroscopic difference between X sperm and Y sperm is evaluated, processed and used to separate the two types of cells. The invention also relates to the development of a cytofluorimeter combining the Raman microscope with a system for trapping optically the sperms and a microfluidic chamber. More precisely, the optical trapping system, equipped with an objective with high numerical aperture, is used to immobilize the cells and, the same objective is used to excite and gather Raman spectra. At last, the microfluidic chamber is used to make the sample to flow, with the final purpose of providing a quick, precise and not invasive separation of X sperm and Y sperm, (figure 5A).
摘要翻译：本发明涉及一种基于使用拉曼光谱法来面对动物物种，特别是牛物种中的X精子和Y精子的生物化学分析和分化问题的方法，所述方法使随后的使用成为可能在fecundation技术。解决这个问题的方法是基于X精子和Y精子在授精前的分离。还描述了一种实现这种方法的装置，其通过以非侵入性和非破坏性的方式获取和分析拉曼光谱来区分和表征X精子和Y精子之间的差异。通过统计方法，例如主要成分的分析和线性鉴别分析，X精子与Y精子之间的光谱差异被评估，处理并用于分离两种细胞。本发明还涉及将拉曼显微镜与用于光学捕获精子和微流体室的系统结合的细胞荧光计的开发。更准确地说，配备有具有高数值孔径的物镜的光学捕获系统用于固定电池，并且使用相同的目的来激发和聚集拉曼光谱。最后，微流体室用于使样品流动，其最终目的是提供X精子和Y精子的快速，精确和不侵入的分离（图5A）。

4. 发明申请

WO2006004804A1 INTERFACE FROM A THERMAL DESORPTION UNIT TO A CHROMATOGRAPHIC COLUMN 审中-公开
标题翻译：从热解吸单元到色谱柱的界面
公开(公告)号：WO2006004804A1
公开(公告)日：2006-01-12
申请号：PCT/US2005/023033
申请日：2005-06-29
申请人： PERKINELMER LAS, INC. , TIPLER, Andrew , GRECSEK, Heidi , DELORENZO, Frank , COPPOLA, Giuseppe
发明人： TIPLER, Andrew , GRECSEK, Heidi , DELORENZO, Frank , COPPOLA, Giuseppe
IPC分类号： G01N30/12
CPC分类号： G01N30/12 , G01N30/16 , G01N30/32 , G01N2030/0015 , G01N2030/128 , G01N2030/324
摘要： Disclosed are systems (10) and methods that include a thermal desorption unit (20), a chromatographic column (24), and an interface device (60) between the unit (20) and the column (24). The interface device (60) controls the fluid flowing into the column, such as by providing additional carrier gas to maintain a substantially constant gas flow or velocity, by proving a controlled temperature increase, or by venting a portion of the gas received from the thermal desorption unit (20), thereby removing the dependence on the thermal desorption unit (20) for such control. In some embodiments, the interface (60) is a chromatographic injector. In certain embodiments, a transfer line (28) from the desorption unit (20) is coupled to the same port of the interface device (60) as the chromatographic column (24). In some of these embodiments, this is accomplished by employing a special adaptor.
摘要翻译：公开了在单元（20）和柱（24）之间包括热解吸单元（20），色谱柱（24）和界面装置（60）的系统（10）和方法。界面装置（60）控制流入柱的流体，例如通过提供额外的载气来维持基本恒定的气流或速度，通过证明受控的温度升高，或通过将从热量接收的气体的一部分解吸单元（20），从而消除了对这种控制的热解吸单元（20）的依赖性。在一些实施例中，界面（60）是色谱注射器。在某些实施方案中，来自解吸单元（20）的输送管线（28）与色谱柱（24）耦合到界面装置（60）的相同端口。在这些实施例中的一些实施例中，这通过采用特殊适配器来实现。

5. 发明申请

WO2006086400A2 ZIRCONIA TOUGHENED ALUMINA COMPOSITION AND USE IN ION AND ELECTRON OPTICAL SYSTEMS 审中-公开
标题翻译： ZIRCONIA强化氧化铝组合物和在离子和电子光学系统中的使用
公开(公告)号：WO2006086400A2
公开(公告)日：2006-08-17
申请号：PCT/US2006/004316
申请日：2006-02-08
申请人： PERKINELMER LAS, INC. , MANNINO, Rosario , COPPOLA, Giuseppe
发明人： MANNINO, Rosario , COPPOLA, Giuseppe
IPC分类号： H01J49/04
CPC分类号： H01J49/00 , C04B35/119 , C04B35/4885
摘要： An ion optical system including an ionizer for ionizing one or more compounds, the ionizer including a plurality of components, a mass separator for separating ions of the one or more compounds, the mass separator including a plurality of components, and a detector for identifying the one or more compounds, the detector including a plurality of components. At least one of the plurality of components of at least one of the ionizer, mass separator and detector includes a composition of zirconium oxide and aluminum oxide.
摘要翻译：一种离子光学系统，包括用于离子化一种或多种化合物的离子发生器，所述离子发生器包括多个组分，用于分离所述一种或多种化合物的离子的质量分离器，所述质量分离器包括多个组分，以及用于鉴定一种或多种化合物，所述检测器包括多个组分。离子发生器，质量分离器和检测器中的至少一个的多个部件中的至少一个包括氧化锆和氧化铝的组成。

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