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    • 2. 发明申请
    • METHOD OF NONLINEAR HARMONIC HOLOGRAPHY
    • 非线性谐波全息法的方法
    • WO2008127432A3
    • 2008-12-11
    • PCT/US2007085409
    • 2007-11-21
    • CALIFORNIA INST OF TECHNPU YEPSALTIS DEMETRI
    • PU YEPSALTIS DEMETRI
    • G03H1/00
    • G01N21/636B82Y20/00G01N21/453G01N21/658G01N2021/653G02B5/32G03H1/0465G03H2001/0447G03H2210/62G03H2210/63G03H2222/33G03H2223/26G03H2240/56
    • A harmonic holography (H2) technique and system that combines holography and nonlinear optics that enables holographic recording of 3D images with femtosecond framing time are provided. The H2 technique records holograms with second harmonic (SH) signals scattered off specialized nanocrystals that are functionalized to label specific protein or other biomolecules in a living organism. The capability of generating second harmonic radiations is specific to materials with noncentrosymmetric crystalline structures only, and ?® vanishes for all other types of materials. Therefore, a sharp contrast is formed when particles of noncentrosymmetric structures are dispersed in a medium of other species, pumped at a fundamental frequency, and imaged at the second harmonic frequency. The new scheme described herein provides a sound basis for a new type of contrast microscopy with enormous potential in molecular biomedical imaging.
    • 提供了一种组合全息和非线性光学的谐波全息(H2)技术和系统,可以实现3D图像与飞秒成帧时间的全息记录。 H2技术记录具有二次谐波(SH)信号的全息图,散射专门的纳米晶体,其被功能化以标记生物体中的特定蛋白质或其他生物分子。 产生二次谐波辐射的能力仅限于具有非中心对称结晶结构的材料,而对于所有其他类型的材料,?®消失。 因此,当非中心对称结构的颗粒分散在其他物质的介质中,以基频泵浦并且以二次谐波频率成像时,形成明显的对比。 本文描述的新方案为分子生物医学成像中具有巨大潜力的新型对比显微镜提供了良好的基础。