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1. 发明申请

WO2008005322A3 SYSTEM AND METHOD FOR RAPID THERMAL CYCLING 审中-公开
公开(公告)号：WO2008005322A3
公开(公告)日：2008-01-10
申请号：PCT/US2007/015106
申请日：2007-06-29
申请人： CANON U.S. LIFE SCIENCES, INC. , ZENG, Shulin , HASSON, Kenton C. , DALE, Gregory A. , KEADY, John
发明人： ZENG, Shulin , HASSON, Kenton C. , DALE, Gregory A. , KEADY, John
IPC分类号： C12M1/34 , C12M3/00
摘要： At least one exemplary embodiment is directed to an apparatus that includes a microfluidic channel and at least one energy absorbing element, where the energy absorbing element is configured to absorb at least a portion of an incident electromagnetic radiation. The absorption of the radiation by the energy absorbing element varies the temperature of a sample in the microfluidic channel.

2. 发明申请

WO2008005321A2 SYSTEMS AND METHODS FOR REAL-TIME PCR 审中-公开
标题翻译：用于实时PCR的系统和方法
公开(公告)号：WO2008005321A2
公开(公告)日：2008-01-10
申请号：PCT/US2007/015104
申请日：2007-06-29
申请人： CANON U.S. LIFE SCIENCES, INC. , OWEN, Gregory H. , DALE, Gregory A. , HASSON, Kenton C. , ZENG, Shulin , WARFIELD, Dwayne W.
发明人： OWEN, Gregory H. , DALE, Gregory A. , HASSON, Kenton C. , ZENG, Shulin , WARFIELD, Dwayne W.
IPC分类号： C12Q1/68 , G06T7/20 , C12M3/00
CPC分类号： B01L7/525 , B01L3/5027 , B01L3/502715 , B01L3/50273 , B01L7/52 , B01L2200/10 , B01L2200/143 , B01L2300/0627 , B01L2300/0654 , B01L2300/0829 , B01L2300/18 , B01L2300/1811 , B01L2300/1822 , B01L2300/1827 , B01L2300/1877 , B01L2400/0487 , C12Q1/686 , G01N21/71 , G01N35/08 , G06T7/0012 , G06T7/11 , G06T2207/10004 , G06T2207/30072 , Y10T436/115831 , Y10T436/117497 , C12Q2565/629 , C12Q2561/113
摘要： In one aspect, the present invention provides a systems and methods for the real-time amplification and analysis of a sample of DNA.
摘要翻译：一方面，本发明提供了用于实时扩增和分析DNA样品的系统和方法。

3. 发明申请

WO2008005323A2 COMBINED THERMAL DEVICES FOR THERMAL CYCLING 审中-公开
标题翻译：用于热循环的组合热装置
公开(公告)号：WO2008005323A2
公开(公告)日：2008-01-10
申请号：PCT/US2007/015109
申请日：2007-06-29
申请人： CANON U.S. LIFE SCIENCES, INC. , DALE, Gregory A. , ZENG, Shulin , HASSON, Kenton C.
发明人： DALE, Gregory A. , ZENG, Shulin , HASSON, Kenton C.
IPC分类号： C12Q1/68 , G06T7/20 , C12M3/00
CPC分类号： B01L7/525 , B01L3/5027 , B01L3/502715 , B01L3/50273 , B01L7/52 , B01L2200/10 , B01L2200/143 , B01L2300/0627 , B01L2300/0654 , B01L2300/0829 , B01L2300/18 , B01L2300/1811 , B01L2300/1822 , B01L2300/1827 , B01L2300/1877 , B01L2400/0487 , C12Q1/686 , G01N21/71 , G01N35/08 , G06T7/0012 , G06T7/11 , G06T2207/10004 , G06T2207/30072 , Y10T436/115831 , Y10T436/117497 , C12Q2565/629 , C12Q2561/113
摘要： The invention relates to systems and methods including a combination of thermal generating device technologies to achieve more efficiency and accuracy in PCR temperature cycling of nucleic samples undergoing amplification.
摘要翻译：本发明涉及包括热生成装置技术的组合的系统和方法，以实现经历扩增的核酸样品的PCR温度循环中的更高效率和准确度。

4. 发明申请

WO2008066869A2 SYSTEMS AND METHODS FOR MONITORING THE AMPLIFICATION AND DISSOCIATION BEHAVIOR OF DNA MOLECULES 审中-公开
标题翻译：用于监测DNA分子的放大和分解行为的系统和方法
公开(公告)号：WO2008066869A2
公开(公告)日：2008-06-05
申请号：PCT/US2007/024538
申请日：2007-11-29
申请人： CANON U.S. LIFE SCIENCES, INC. , HASSON, Kenton C. , DALE, Gregory A. , KEADY, John P.
发明人： HASSON, Kenton C. , DALE, Gregory A. , KEADY, John P.
IPC分类号： G06K9/00
CPC分类号： C12Q1/686 , B01L3/5027 , G01N21/6452 , G01N21/6454 , G01N21/6456 , H01L27/146
摘要： The present invention relates to systems and methods for monitoring the amplification of DNA molecules and the dissociation behavior of the DNA molecules.
摘要翻译：本发明涉及用于监测DNA分子扩增的系统和方法以及DNA分子的解离行为。

5. 发明申请

WO2009032087A1 MICROFLUIDIC DEVICES WITH INTEGRATED RESISTIVE HEATER ELECTRODES 审中-公开
标题翻译：具有集成电阻加热器电极的微流体装置
公开(公告)号：WO2009032087A1
公开(公告)日：2009-03-12
申请号：PCT/US2008/010032
申请日：2008-08-22
申请人： CANON U.S. LIFE SCIENCES, INC. , HANAGATA, Takayoshi , INOUE, Hiroshi , DALE, Gregory A. , HASSON, Kenton C. , OWEN, Gregory H. , COURSEY, Johnathan S.
发明人： HANAGATA, Takayoshi , INOUE, Hiroshi , DALE, Gregory A. , HASSON, Kenton C. , OWEN, Gregory H. , COURSEY, Johnathan S.
IPC分类号： F15C5/00
CPC分类号： B01L7/52 , B01L3/5027 , B01L2200/147 , B01L2200/148 , B01L2300/0654 , B01L2300/0861 , B01L2300/0887 , B01L2300/1827 , G05D23/1934 , G05D23/22 , G05D23/24
摘要： The invention relates to methods and devices for control of an integrated thin-film device with a plurality of microfluidic channels. In one embodiment, the microfluidic device includes a microfluidic chip comprising a first zone having a plurality of microfluidic channels and a second zone having a plurality of microfluidic channels, wherein the microfluidic channels in the first and second zones are in fluid communication. The microfluidic device further comprising a thin-film heater in thermal communication with each of the microfluidic channels in the first and second zones. The microfluidic device also includes a control system configured to independently control the temperature of each of the thin-film heaters using pulse width modulation (PWM) control signals that are optimized for each of the thin-film heaters.
摘要翻译：本发明涉及用于控制具有多个微流体通道的集成薄膜器件的方法和装置。在一个实施例中，微流体装置包括微流体芯片，其包括具有多个微流体通道的第一区域和具有多个微流体通道的第二区域，其中第一区域和第二区域中的微流体通道是流体连通的。微流体装置还包括与第一和第二区域中的每个微流体通道热连通的薄膜加热器。微流体装置还包括控制系统，其被配置为使用针对每个薄膜加热器优化的脉冲宽度调制（PWM）控制信号独立地控制每个薄膜加热器的温度。

6. 发明申请

WO2008005241A3 SYSTEMS AND METHODS FOR MONITORING THE AMPLIFICATION AND DISSOCIATION BEHAVIOR OF DNA MOLECULES 审中-公开
公开(公告)号：WO2008005241A3
公开(公告)日：2008-01-10
申请号：PCT/US2007/014827
申请日：2007-06-27
申请人： CANON U.S. LIFE SCIENCES, INC. , HASSON, Kenton C. , DALE, Gregory A.
发明人： HASSON, Kenton C. , DALE, Gregory A.
IPC分类号： C12M1/34 , C12P19/34
摘要： The present invention relates to systems and methods for monitoring the amplification of DNA molecules and the dissociation behavior of the DNA molecules. A method according to one embodiment of the invention may include the steps of : forcing a sample of a solution containing real-time PCR reagents to move though a channel; and while the sample is moving through an analysis region of the channel, performing the steps of: (a) cycling the temperature of the sample until the occurrence of a predetermined event; (b) after performing step (a), causing the sample' s temperature to gradually' increase from a first temperature to a second temperature; and (c) while the step of gradually increasing the sample' s temperature is performed, using an image sensor to monitor emissions from the sample.

7. 发明申请

WO2008066859A1 DEVICE FOR NUCLEIC ACID PREPARATION 审中-公开
标题翻译：核酸制备装置
公开(公告)号：WO2008066859A1
公开(公告)日：2008-06-05
申请号：PCT/US2007/024524
申请日：2007-11-29
申请人： CANON U.S. LIFE SCIENCES, INC. , KNIGHT, Ivor T. , STONE, Michele , DALE, Gregory A.
发明人： STONE, Michele , DALE, Gregory A.
IPC分类号： C12Q1/68 , C12N13/00 , C12M3/08
CPC分类号： C12M47/06 , Y10T436/255
摘要： A device for nucleic acid preparation includes an outer housing and an inner housing carried within the outer housing and rotatable with respect to the outer housing. The inner housing defines an inner chamber and includes inlet and outlet ports and a nucleic acid extraction substrate within the inner chamber. The outer housing includes a sample inlet port, wash fluid inlet and outlet ports, and elute fluid inlet and outlet ports, and the ports of the inner housing can be selectively aligned with ports of the outer housing by rotating the inner housing with respect to the outer housing. Nucleic acid from the sample introduced into the chamber is captured on the extraction substrate and is released from the extraction substrate by sonication prior to being eluted out of the chamber.
摘要翻译：用于核酸制备的装置包括外部壳体和承载在外部壳体内并可相对于外部壳体旋转的内部壳体。内壳体限定内腔，并且包括入口和出口以及内腔内的核酸提取基底。外壳包括样品入口，洗涤流体入口和出口以及洗脱液入口和出口，并且内壳体的端口可以通过相对于内壳体旋转内壳而与外壳体的端口选择性地对准外壳。从引入到室中的样品中的核酸被捕获在提取基质上，并且在从室中洗出之前通过超声处理从提取基质中释放出来。

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