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    • 1. 发明申请
    • HANDHELD LOW PRESSURE MECHANICAL CELL LYSIS DEVICE WITH SINGLE CELL RESOLUTION
    • 单电池解决方案的手持低压机械电解槽装置
    • WO2012037061A2
    • 2012-03-22
    • PCT/US2011051298
    • 2011-09-13
    • CALIFORNIA INST OF TECHNLI ZHENYUSHERER AXEL
    • LI ZHENYUSHERER AXEL
    • C12N1/06C12M1/33C12Q1/02
    • C12M47/06C12N1/066
    • Apparatus and methods for mechanical cell lysis with single cell resolution which requires very low applied pressure. The device can be handheld, simple to operate, requires no external power except for hand-applied pressure via a syringe, and is applicable to all cell types including yeast and bacterial cells. The device is also capable of mechanically lysing a single cell. A single cell is selected from a biological sample of interest. The single cell is lysed by application of mechanical stress in a single cell lysing apparatus having a trap structure for deterministically capturing the cell and a stress raiser that cooperates with a source of mechanical stress so as to apply sufficient force to rupture a cell. The stress raiser can be a properly designed edge of the trap or it can be a lithographically produced structure such as a nanoblade or a nanopillar.
    • 用于需要非常低施加压力的具有单细胞分辨率的机械细胞溶解的装置和方法。 该设备可手持,操作简单,除了通过注射器手动施加压力外不需要外部电源,适用于所有细胞类型,包括酵母和细菌细胞。 该装置还能够机械地裂解单个细胞。 从感兴趣的生物样品中选择单个细胞。 通过在具有陷阱结构的单细胞裂解装置中施加机械应力来裂解单细胞,所述装置具有用于确定性地捕获细胞的陷阱结构以及与机械应力源协作的应力提升器,以施加足够的力来使细胞破裂。 应力提升器可以是陷阱的适当设计的边缘,或者它可以是光刻产生的结构,例如纳米刀片或纳米柱。
    • 2. 发明申请
    • MECHANICALLY TUNABLE ELASTOMERIC OPTOFLUIDIC DISTRIBUTED FEEDBACK DYE LASERS
    • 机械柔性弹性光滑分布式反馈染料激光
    • WO2008105914A3
    • 2008-12-18
    • PCT/US2007075668
    • 2007-08-10
    • CALIFORNIA INST OF TECHNLI ZHENYUPSALTIS DEMETRISCHERER AXELZHANG ZHAOYU
    • LI ZHENYUPSALTIS DEMETRISCHERER AXELZHANG ZHAOYU
    • H01S3/213H01S3/094
    • H01S3/022H01S3/08036H01S3/1053H01S3/213
    • The invention relates to a microfluidic dye laser (100) including a pump light source configured to provide light (107) having a pump light wavelength. The microfluidic dye laser (100) also includes an elastomer substantially optically transparent at the pump light wavelength (107) and at a microfluidic dye laser wavelength. A microfluidic channel (102) configured to accept a iluidic dye is defined in the elastomer. An optical grating (108) is formed in a single mode 3D waveguide in the microfluidic channel (102) in order to provide a single mode microfluidic dye laser light as output in response to illumination with light from the pump light source (107). In another aspect, the invention features a method of tuning a wavelength of a microfluidic dye laser light by mechanically deforming the eiastomeric laser chip to change the grating period in the optical cavity.
    • 本发明涉及一种微流体染料激光器(100),其包括被配置为提供具有泵浦光波长的光(107)的泵浦光源。 微流体染料激光器(100)还包括在泵浦光波长(107)处以微流体染料激光波长基本上光学透明的弹性体。 在弹性体中限定了构造成接受液体染料的微流体通道(102)。 在微流体通道(102)中的单模3D波导中形成光栅(108),以便响应于来自泵浦光源(107)的光的照明,提供单模微流体染料激光作为输出。 在另一方面,本发明的特征在于一种通过机械地使所述激光激光芯片变形以改变光腔中的光栅周期来调谐微流体染料激光的波长的方法。
    • 5. 发明申请
    • RANGE OF ENGINES USING COMMON RAIL FUEL SYSTEM WITH PUMP AND RAIL ASSEMBLIES HAVING COMMON COMPONENTS
    • 使用普通铁路燃油系统的发动机范围与带有共同部件的泵和导轨组件
    • WO2011109325A3
    • 2011-12-01
    • PCT/US2011026578
    • 2011-03-01
    • CATERPILLAR INCLI ZHENYUSHAFER SCOTT F
    • LI ZHENYUSHAFER SCOTT F
    • F02M55/02F02M59/00F02M59/44F02M61/14
    • F02M63/0285
    • A pump and rail assembly (54) includes a plurality of quills (84) that are compressed between quill seats (108, 109) on a pump (75) and an output rail (76). A rail pressure control valve (77) and a rail pressure sensor (79) are attached to respective ends of the output rail (76). An inlet throttle valve (95) is attached to a housing (100) of the pump and rail assembly (54). Depending upon the application, the output rail (76) may supply fuel to a first injection bank (70) that includes a plurality of fuel injectors (72) in a first common rail (49, 50), and a second fuel injection bank that includes a second common rail (52, 53) and a plurality of fuel injectors (72). Different engines having different numbers of cylinders (11, 16, 21, 26, 27, 33, 35, 41, 43, 48, 51, 57, 60) may use similar pump and rail assemblies that each include a plurality of quills (84), a plurality of pumping elements (94) positioned in a pump housing (100) and an output rail (76). The quills (84) for each of the different engine applications are interchangeable but differ in number. In addition, the pumping elements (94) of each of the different engine applications are also interchangeable but differ in number.
    • 泵和轨道组件(54)包括多个在泵(75)上的套筒座(108,109)和输出轨道(76)之间被压缩的套管(84)。 导轨压力控制阀(77)和导轨压力传感器(79)连接到输出导轨(76)的相应端部。 入口节流阀(95)连接到泵和轨道组件(54)的壳体(100)。 取决于应用,输出轨道76可将燃料供应到包括第一共轨(49,50)中的多个燃料喷射器(72)的第一喷射组(70)和第二燃料喷射组 包括第二共轨(52,53)和多个燃料喷射器(72)。 具有不同数量气缸(11,16,21,26,27,33,35,41,43,48,51,57,60)的不同发动机可以使用类似的泵和导轨组件,每个导管组件包括多个套管(84 ),定位在泵壳体(100)和输出轨道(76)中的多个泵送元件(94)。 针对每种不同的发动机应用的套管(84)是可互换的,但数量不同。 另外,不同发动机应用中的每一个的泵送元件(94)也可互换,但数量不同。