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1. 发明申请

WO2006099605A2 DIMERIC AND TRIMERIC NUCLEIC ACID DYES, AND ASSOCIATED SYSTEMS AND METHODS 审中-公开
标题翻译：二聚和三聚体酸性染料及相关系统和方法
公开(公告)号：WO2006099605A2
公开(公告)日：2006-09-21
申请号：PCT/US2006/009910
申请日：2006-03-17
申请人： BIOTIUM, INC. , ALLELOGIC BIOSCIENCES CORP , MAO, Fei , LEUNG, Wai-Yee , XIN, Xing
发明人： MAO, Fei , LEUNG, Wai-Yee , XIN, Xing
IPC分类号： C12Q1/68 , C07D401/02
CPC分类号： C12Q1/6876 , C07D209/08 , C07D213/38 , C07D221/10 , C07D417/06 , C07D417/14 , C07D491/147 , C07H21/00 , C09B15/00 , C12Q1/6851 , C12Q1/686 , C12Q2563/173
摘要： Dimeric and trimeric nucleic acid dyes, and associated systems and methods are provided. Such a dye may form a hairpin-like structure that enables it to stain nucleic acids via a release-on-demand mechanism, for example. Such a dye may have low background fluorescence in the absence of nucleic acids and high fluorescence in the presence of nucleic acids, upon binding therewith, for example. A dye provided herein may be useful in a variety of applications, such as in DNA quantitation in real-time PCR, for example.
摘要翻译：提供二聚和三聚体核酸染料以及相关系统和方法。这样的染料可以形成发夹状结构，使其能够通过例如按需释放机制来染色核酸。在不存在核酸的情况下，这样的染料可能具有低的背景荧光，并且例如在与核酸的结合时在核酸存在下具有高荧光。本文提供的染料可用于各种应用，例如在实时PCR中的DNA定量中。

2. 发明申请

WO2010002476A1 COMPOSITIONS AND METHODS FOR THE PROTECTION OF NUCLEOPHILIC GROUPS 审中-公开
标题翻译：组成和保护核酸组的方法
公开(公告)号：WO2010002476A1
公开(公告)日：2010-01-07
申请号：PCT/US2009/003942
申请日：2009-07-02
申请人： ALLELOGIC BIOSCIENCES CORPORATION , MAO, Fei , XIN, Xing , LEUNG, Wai-Yee
发明人： MAO, Fei , XIN, Xing , LEUNG, Wai-Yee
IPC分类号： C12P19/34 , C12N9/99 , C08H1/00
CPC分类号： C12Q1/6844 , C07K1/064 , C08H1/00 , C12N9/1252 , C12N9/127 , C12P19/34 , C12Q2521/101 , C12Q2521/119
摘要： The present invention provides compositions, methods, and kits relating to the protection and deprotection of molecules comprising nucleophilic groups, such as the protection and deprotection of thermostable polymerases. Also provided are methods of performing nucleic acid amplification using polymerases protected according to the invention.
摘要翻译：本发明提供了与包含亲核基团的分子的保护和去保护有关的组合物，方法和试剂盒，例如耐热聚合酶的保护和去保护。还提供使用根据本发明保护的聚合酶进行核酸扩增的方法。

3. 发明申请

WO2007038422A2 OLIGONUCLEOTIDES AS TEMPERATURE-SENSITIVE INHIBITORS FOR DNA POLYMERASES 审中-公开
标题翻译：寡核苷酸作为温度敏感的DNA聚合酶抑制剂
公开(公告)号：WO2007038422A2
公开(公告)日：2007-04-05
申请号：PCT/US2006/037238
申请日：2006-09-26
申请人： ALLELOGIC BIOSCIENCES CORP. , MAO, Fei , XIN, Xing
发明人： MAO, Fei , XIN, Xing
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6848 , C12Q2527/101 , C12Q2525/205
摘要： Aspects of the invention relate to the use of novel oligonucleotides as temperature-sensitive inhibitors for thermostable DNA polymerases. Some inhibitors exhibit temperature-dependent and, in some cases, reversible inhibitory property by changing the conformation of at least a portion of the oligonucleotides from double-stranded to single stranded or in some cases vice versa in a temperature-dependent manner. Aspects also relate to the use of an the inhibitors in a hot-start PCR compositions, wherein the inhibitor may act to suppress the activity of the thermostable DNA polymerase below a desired activation temperature, Tact, and wherein the inhibitor is thermally inactivated above Tact, thus liberating the polymerase activity and initiating the DNA amplification process. Aspects further relate to a procedure for formulating the composition of a hot-start PCR reaction mixture. The hot-start PCR methods disclosed herein are generally faster, more flexible and lower in cost than existing methods.
摘要翻译：本发明的方面涉及使用新的寡核苷酸作为热稳定性DNA聚合酶的温度敏感性抑制剂。一些抑制剂通过将至少一部分寡核苷酸的构象从双链改变成单链或者在一些情况下以温度依赖性方式反过来表现出温度依赖性并且在一些情况下具有可逆的抑制性质。各方面还涉及在热启动PCR组合物中使用抑制剂，其中所述抑制剂可用于抑制热稳定性DNA聚合酶的活性低于期望的活化温度Tact，并且其中所述抑制剂在Tact以上热灭活，从而释放聚合酶活性并启动DNA扩增过程。各方面还涉及配制热启动PCR反应混合物的组成的程序。本文公开的热启动PCR方法通常比现有方法更快，更灵活并且成本更低。

4. 发明申请

WO2005051967A3 OLIGONUCLEOTIDES LABELED WITH A PLURALITY OF FLUOROPHORES 审中-公开
公开(公告)号：WO2005051967A3
公开(公告)日：2005-06-09
申请号：PCT/US2004/038946
申请日：2004-11-19
申请人： ALLELOGIC BIOSCIENCES CORP. , MAO, Fei , XIN, Xing
发明人： MAO, Fei , XIN, Xing
IPC分类号： C07H21/02 , C07H21/04 , C12Q1/68
摘要： An embodiment of the invention discloses new methods for designing labeled nucleic acid probes and primers by labeling oligonucleotides with a plurality of spectrally identical or similar dyes and optionally with one or more quencher dyes. Oligonucleotides labeled in accordance with some embodiments of the invention exhibit a detectable increase in signal, for example, fluorescent signal when the labeling dyes are separated from one another. Methods for separating the dye include cleaving the labeled oligonucleotides include using enzymes that have 5'-exonuclease activity. In one embodiment nucleic acid primers of the present invention may fluoresce upon hybridization to a target sequence and incorporation into the amplification product. Nucleic acid probes and primers of the present invention have wide applications ranging from general detection of a target nucleic acid sequence to clinical diagnostics. Major advantages of the oligonucleotides including nucleic acid probes and primers of many embodiments of the present invention are their synthetic simplicity, spectral versatility and superior fluorescent signal.

5. 发明申请

WO2010036359A2 METHODS OF POLYMERASE ACTIVITY ASSAY 审中-公开
标题翻译：聚合酶活性测定方法
公开(公告)号：WO2010036359A2
公开(公告)日：2010-04-01
申请号：PCT/US2009/005326
申请日：2009-09-25
申请人： ALLELOGIC BIOSCIENCES CORPORATION , XIN, Xing , MAO, Fei
发明人： XIN, Xing , MAO, Fei
IPC分类号： C12Q1/68
CPC分类号： C12Q1/48 , C12Q1/6851 , G01N2333/9126 , G01N2500/04 , C12Q2563/173 , C12Q2561/113 , C12Q2525/301
摘要： The present invention provides compositions, methods, and kits for determining the activity of a nucleic acid polymerase by using fluorescent double stranded DNA binding dye and oligonucleotides.
摘要翻译：本发明提供了通过使用荧光双链DNA结合染料和寡核苷酸来确定核酸聚合酶的活性的组合物，方法和试剂盒。

6. 发明申请

WO2009038548A1 OLIGONUCLEOTIDE PROBES AND USES THEREOF 审中-公开
标题翻译：寡核苷酸探针及其用途
公开(公告)号：WO2009038548A1
公开(公告)日：2009-03-26
申请号：PCT/US2007/016500
申请日：2007-07-19
申请人： ALLELOGIC BIOSCIENCES CORPORATION , XIN, Xing , MAO, Fei
发明人： XIN, Xing , MAO, Fei
IPC分类号： C07H19/04 , C07H21/00 , C07H21/02 , C07H21/04 , C12Q1/68 , C12P19/34 , C07D265/34
CPC分类号： C07H19/04 , C07H21/00 , C07H21/02 , C07H21/04 , C12Q1/6851 , C12Q2561/101 , C12Q2527/107
摘要： The present invention provides a dually labeled oligonucleotide probe, methods of preparing and using the same. The subject probes are particularly useful for high-sensitive nucleic acid detection via hybridization assays including but not limited to template-directed polymerization reactions.
摘要翻译：本发明提供双重标记的寡核苷酸探针，其制备和使用方法。本发明探针通过杂交测定法对于高敏感性核酸检测特别有用，包括但不限于模板指导的聚合反应。

7. 发明申请

WO2009042728A1 DETECTION AND /OR QUANTIFICATION OF NUCLEIC ACIDS 审中-公开
标题翻译：检测和/或定量核酸
公开(公告)号：WO2009042728A1
公开(公告)日：2009-04-02
申请号：PCT/US2008/077582
申请日：2008-09-24
申请人： ALLELOGIC BIOSCIENCES CORPORATION , XIN, Xing
发明人： XIN, Xing
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6883 , C12Q1/6827 , C12Q1/6886 , C12Q2600/156 , C12Q2565/632 , C12Q2563/173 , C12Q2545/101 , C12Q2531/113 , C12Q2527/107
摘要： The present invention provides compositions, methods, and kits for nucleic acids analyses. In particular, melting analyses are used to detect the presence or absence and to quantify nucleic acids.
摘要翻译：本发明提供了用于核酸分析的组合物，方法和试剂盒。特别地，使用熔解分析来检测核酸的存在或不存在以及定量。

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