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1. 发明申请

WO2004101743A2 THREE DIMENSIONAL CELL CULTURES IN A MICROSCALE FLUID HANDLING SYSTEM 审中-公开
标题翻译：微尺度流体处理系统中的三维细胞培养
公开(公告)号：WO2004101743A2
公开(公告)日：2004-11-25
申请号：PCT/US2004/014092
申请日：2004-05-06
申请人： BELLBROOK LABS, LLC , LOWERY, Robert, G. , MAJER, John , BEEBE, David, J.
发明人： LOWERY, Robert, G. , MAJER, John , BEEBE, David, J.
IPC分类号： C12N
CPC分类号： C12M23/16
摘要： The present invention provides a novel microscale fluid handling system for initiating, culturing, manipulating and assaying three-dimensional multicellular assembly. The system including a microfluidic device and three-dimensional multicellular tissue surrogate assembly. The device of the invention includes at least one microfluidic channel; and at least one chamber, wherein the walls of the chamber are lined with a cell layer; and wherein fluid medium flows through each of the channels and chambers. Also, disclosed are methods for using the device to introducing test agents to the multicellular assemblies to observe biological responses thereof.
摘要翻译：本发明提供了一种用于启动，培养，操作和测定三维多细胞组装的新型微量流体处理系统。该系统包括微流体装置和三维多细胞组织替代装置。本发明的装置包括至少一个微流体通道; 和至少一个室，其中所述室的壁衬有电池层; 并且其中流体介质流过每个通道和腔室。还公开了使用该装置将测试剂引入多细胞组件以观察其生物反应的方法。

2. 发明申请

WO2010091246A2 BURSTABLE LIQUID PACKAGING AND USES THEREOF 审中-公开
标题翻译：可爆液体包装及其用途
公开(公告)号：WO2010091246A2
公开(公告)日：2010-08-12
申请号：PCT/US2010/023310
申请日：2010-02-05
申请人： NORTHWESTERN UNIVERSITY , KELSO, David, M. , AGARWAL, Abhishek, K. , SUR, Kunal , BEEBE, David, J.
发明人： KELSO, David, M. , AGARWAL, Abhishek, K. , SUR, Kunal , BEEBE, David, J.
IPC分类号： B65B61/00 , B01J14/00
CPC分类号： B65B47/04 , B01L3/502715 , B01L3/52 , B01L3/523 , B01L3/527 , B01L7/52 , B01L2200/027 , B01L2200/04 , B01L2200/0684 , B01L2200/0689 , B01L2200/12 , B01L2200/16 , B01L2300/041 , B01L2300/044 , B01L2300/0609 , B01L2300/0887 , B01L2300/14 , B01L2300/161 , B01L2400/0481 , B01L2400/0683 , B29C65/04 , B29C65/08 , B29C65/16 , B29C65/18 , B29C65/38 , B29C65/76 , B29C65/7808 , B29C66/112 , B29C66/131 , B29C66/242 , B29C66/24221 , B29C66/53461 , B29C66/71 , B29C66/712 , B29C66/72321 , B29C66/72341 , B29C66/8167 , B29C66/8322 , B29C66/84121 , B29K2023/12 , B29K2305/02 , B29L2009/003 , B29L2031/7164 , B32B3/28 , B32B7/045 , B32B7/06 , B32B15/08 , B32B15/20 , B32B2307/31 , B32B2307/71 , B32B2307/7244 , B32B2307/7246 , B32B2307/7265 , B32B2307/728 , B32B2439/80 , B65B7/2842 , B65B29/10 , B65D83/0055 , G01N33/50
摘要： The present invention relates to systems, devices, and methods for performing biological and chemical reactions. In particular, the present invention relates to the use of burstable liquid packaging for delivery of reagents to biological and chemical assays.
摘要翻译：本发明涉及用于进行生物和化学反应的系统，装置和方法。特别地，本发明涉及使用可爆裂液体包装将试剂递送到生物和化学测定。

3. 发明申请

WO2002083390A1 METHOD OF FABRICATING A MICROSTRUCTURE 审中-公开
标题翻译：制作微结构的方法
公开(公告)号：WO2002083390A1
公开(公告)日：2002-10-24
申请号：PCT/US2002/012030
申请日：2002-04-17
申请人： WISCONSIN ALUMNI RESEARCH FOUNDATION , BEEBE, David, J. , BAUER, Joseph, M.
发明人： BEEBE, David, J. , BAUER, Joseph, M.
IPC分类号： B29C33/42
CPC分类号： B29C59/026 , B29C59/022 , B29C2059/023 , Y10S264/44
摘要： A method of fabricating a microstructure is provided. The method includes the step of providing a layer of a polyermizable material (12) in a barrier (28), on the upper surface (25) of a PDMS member (26). The method provides a solid object (16) connected to a shaft (18), and having a plurality of independently positionable legs (22a, 22b, 22c, 22d, 22e) each having a terminal end (36). The legs (22a-22e) of the solid object (16) are brought into contact with the layer of polymerizable material, and may include creating a depression (31) in the PDMS member (26) so as to alter the shape of the upper surface (24) of the layer of material (12). Thereafter, the layer of polymerizable material (12) is polymerized such that the layer solidifies and the upper surface (24) thereof assumes a desired three-dimensional configuration.
摘要翻译：提供了一种制造微结构的方法。该方法包括在PDMS构件（26）的上表面（25）上在阻挡层（28）中提供多层可熔化材料层（12）的步骤。该方法提供连接到轴（18）的固体物体（16），并且具有多个可独立定位的腿（22a，22b，22c，22d，22e），每个腿具有终端（36）。固体物体（16）的腿部（22a-22e）与可聚合材料层接触，并且可以包括在PDMS构件（26）中形成凹陷（31），以改变上部材料层（12）的表面（24）。此后，可聚合材料层（12）聚合成使得层固化，并且其上表面（24）呈现所需的三维构型。

4. 发明申请

WO2012116159A2 DEVICE FOR AND METHOD OF EXTRACTING A FRACTION FROM A BIOLOGICAL SAMPLE 审中-公开
标题翻译：从生物样品中提取分离物的装置和方法
公开(公告)号：WO2012116159A2
公开(公告)日：2012-08-30
申请号：PCT/US2012/026287
申请日：2012-02-23
申请人： WISCONSIN ALUMNI RESEARCH FOUNDATION , BEEBE, David, J. , BERRY, Scott, M. , BURGESS, Richard , STROTMAN, Lindsay
发明人： BEEBE, David, J. , BERRY, Scott, M. , BURGESS, Richard , STROTMAN, Lindsay
CPC分类号： B01D21/0009 , B01D2221/10 , B03C1/288 , B03C2201/18 , B03C2201/26 , C07K1/145 , C07K1/22 , C12N15/1006 , Y10T436/25375 , Y10T436/2575
摘要： A device and method are provided for facilitating extraction of a fraction from a biological sample. The biological sample includes non-desired material and a fraction-bound solid phase substrate. The device includes an input zone for receiving the biological sample therein and a phase-gate zone for receiving an isolation buffer therein. An output zone receives a reagent therein. A force is movable between a first position adjacent the input zone and a second position adjacent the output zone. The force urges the fraction-bound solid phase substrate from the input zone, through the phase-gate zone and into the output zone.
摘要翻译：提供了一种用于促进从生物样品中提取级分的装置和方法。生物样品包括非期望的材料和分数结合的固相底物。该装置包括用于在其中接收生物样本的输入区和用于在其中接收隔离缓冲器的相门区。输出区在其中接收试剂。力可在邻近输入区的第一位置与邻近输出区的第二位置之间移动。力从输入区域通过相位闸区促进分数结合的固相基板进入输出区域。

5. 发明申请

WO2008127818A2 METHOD OF PUMPING FLUID THROUGH A MICROFLUIDIC DEVICE 审中-公开
标题翻译：通过微流体装置泵送流体的方法
公开(公告)号：WO2008127818A2
公开(公告)日：2008-10-23
申请号：PCT/US2008/056621
申请日：2008-03-12
申请人： WISCONSIN ALUMNI RESEACH FOUNDATION , BEEBE, David, J. , WARRICK, Jay, W. , TOEPKE, Michael, W. , MEYVANTSSON, Ivar , WALKER, Glenn, M.
发明人： BEEBE, David, J. , WARRICK, Jay, W. , TOEPKE, Michael, W. , MEYVANTSSON, Ivar , WALKER, Glenn, M.
IPC分类号： G01N27/26 , G01N27/44 , B81B1/00 , B01L3/00
CPC分类号： F04B19/006 , B01L3/50273 , B01L2300/0816 , B01L2400/0406 , B01L2400/0457 , Y10T137/0357 , Y10T137/0363 , Y10T137/0379 , Y10T137/0396 , Y10T436/11 , Y10T436/117497 , Y10T436/118339 , Y10T436/2575
摘要： A method is provided for pumping fluid through a channel (22) of a microfluidic device (10). The channel (22) has an input port (28) and an output port (32). The channel (22) is filled with fluid and a pressure gradient is generated between the fluid at the input port (28) and the fluid at the output port (28). As a result, fluid flows through the channel (22) towards the output port (32).
摘要翻译：提供了一种用于泵送流体通过微流体装置（10）的通道（22）的方法。通道（22）具有输入端口（28）和输出端口（32）。通道（22）充满流体并且在输入端口（28）处的流体与输出端口（28）处的流体之间产生压力梯度。结果，流体通过通道（22）流向输出端口（32）。

6. 发明申请

WO2006121667A3 DEVICE AND METHOD FOR PERFORMING A HIGH THROUGHPUT ASSAY 审中-公开
公开(公告)号：WO2006121667A3
公开(公告)日：2006-11-16
申请号：PCT/US2006/016547
申请日：2006-05-01
申请人： WISCONSIN ALUMNI RESEARCH FOUNDATION , BEEBE, David, J. , MEYVANTSSON, Ivar
发明人： BEEBE, David, J. , MEYVANTSSON, Ivar
IPC分类号： G01N31/22
摘要： A device and method is provided for performing a high throughput assay. The device includes a plate structure having a plate (2) and a plurality of microfluidic structures (10) positioned thereon. Each microfluidic structure (10) defines a channel (22) having an input (28) and an output (32). At least one of the input (28) and the output (32) of the channel (22) of each of the plurality of microfluidic structures (10) includes a first plurality of ports (28a). In operation, the channels (22) are filled with fluid and pressure gradients are generated between the fluids at the inputs (28) and the fluids at the outputs (32) of the channels (22). As a result, fluid flows through the channels (22) toward the outputs (32).

7. 发明申请

WO2011106044A1 DEVICE FOR AND METHOD OF EXTRACTING A FRACTION FROM A BIOLOGICAL SAMPLE 审中-公开
标题翻译：从生物样品中提取分离物的装置和方法
公开(公告)号：WO2011106044A1
公开(公告)日：2011-09-01
申请号：PCT/US2010/055980
申请日：2010-11-09
申请人： WISCONSIN ALUMNI RESEARCH FOUNDATION , BEEBE, David, J. , BERRY, Scott, M.
发明人： BEEBE, David, J. , BERRY, Scott, M.
IPC分类号： C12M1/32 , C12S3/20 , C12Q1/68 , G01N33/48
CPC分类号： C07K1/145 , C07K1/22
摘要： A device and method are provided for facilitating extraction of a fraction from a biological sample. The biological sample includes non-desired material and a fraction-bound solid phase substrate. The device includes an input zone for receiving the biological sample therein and a phase-gate zone for receiving an isolation buffer therein. An output zone receives a reagent therein. A force is movable between a first position adjacent the input zone and a second position adjacent the output zone. The force urges the fraction-bound solid phase substrate from the input zone, through the phase-gate zone and into the output zone.
摘要翻译：提供了一种用于促进从生物样品中提取级分的装置和方法。生物样品包括非期望的材料和分数结合的固相底物。该装置包括用于在其中接收生物样本的输入区和用于在其中接收隔离缓冲器的相门区。输出区在其中接收试剂。力可在邻近输入区的第一位置与邻近输出区的第二位置之间移动。力从输入区域通过相位闸区促进分数结合的固相基板进入输出区域。

8. 发明申请

WO2003062920A3 ELASTOMERIC STENCILS FOR MICROPATTERNING CELLS 审中-公开
公开(公告)号：WO2003062920A3
公开(公告)日：2003-07-31
申请号：PCT/US2002/026003
申请日：2002-08-15
申请人： THE GENERAL HOSPITAL CORPORATION , THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS , TONER, Mehmet , FOLCH, Albert , BEEBE, David, J.
发明人： TONER, Mehmet , FOLCH, Albert , BEEBE, David, J.
IPC分类号： C12Q1/34
摘要： The present invention provides new fabrication methods and techniques for making and using stencils to micropattern cells. These techniques may allow the fabrication of thinner stencils. The invention provides a system for selectively applying cells to predetermined regions on a substrate. A stencil is positioned adjacent to a substrate to cover some portions of the substrate, while allowing other portions of the substrate to remain uncovered. The stencil may have one or more channels passing through the stencil, and in certain cases, the channels may have a cross-sectional dimension of less than 1 mm. The stencil may be applied to a cell culture substrate in such a way as to form a microscopic cellular pattern on the substrate, and the stencil may form a seal or other watertight contact with the substrate in some cases. In one embodiment, the stencil may be applied to a substrate before seeding. Cells are applied to the uncovered portions of the surface, then the stencil may be removed if desired. The stencil may be fabricated, for example, by injecting an elastomeric precursor into a mold, or by curing an elastomeric precursor while applying pressure. In some embodiments, the surface of the stencil may also be oxidized, or it may be treated in such a way as to render the surface of the stencil more hydrophilic, for example, by treating the surface using oxygen-plasma treatment.

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