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1. 发明申请

WO03062920A2 ELASTOMERIC STENCILS FOR MICROPATTERNING CELLS 审中-公开
标题翻译：微型细胞的弹性模板
公开(公告)号：WO03062920A2
公开(公告)日：2003-07-31
申请号：PCT/US0226003
申请日：2002-08-15
申请人： GEN HOSPITAL CORP , UNIV ILLINOIS , TONER MEHMET , FOLCH ALBERT , BEEBE DAVID J
发明人： TONER MEHMET , FOLCH ALBERT , BEEBE DAVID J
IPC分类号： G06T7/00 , G03F
CPC分类号： B01L3/5085 , B01L2200/12 , B01L2300/069 , B01L2300/0819 , B01L2300/0829 , B01L2300/0887 , B01L2300/0893 , B01L2300/123 , C12M23/12
摘要： The present invention provides new fabrication methods and techniques for making and using stencils to micropattern cells. These techniques may allow the fabrication of thinner stencils. The invention provides a system for selectively applying cells to predetermined regions on a substrate. A stencil is positioned adjacent to a substrate to cover some portions of the substrate, while allowing other portions of the substrate to remain uncovered. The stencil may have one or more channels passing through the stencil, and in certain cases, the channels may have a cross-sectional dimension of less than 1 mm. The stencil may be applied to a cell culture substrate in such a way as to form a microscopic cellular pattern on the substrate, and the stencil may form a seal or other watertight contact with the substrate in some cases. In one embodiment, the stencil may be applied to a substrate before seeding. Cells are applied to the uncovered portions of the surface, then the stencil may be removed if desired. The stencil may be fabricated, for example, by injecting an elastomeric precursor into a mold, or by curing an elastomeric precursor while applying pressure. In some embodiments, the surface of the stencil may also be oxidized, or it may be treated in such a way as to render the surface of the stencil more hydrophilic, for example, by treating the surface using oxygen-plasma treatment.
摘要翻译：本发明提供了制造和使用模板到微图案单元的新制造方法和技术。这些技术可以允许制造更薄的模板。本发明提供了一种用于选择性地将细胞施加到基底上的预定区域的系统。将模版定位在衬底附近以覆盖衬底的一些部分，同时允许衬底的其他部分保持未被覆盖。模板可具有一个或多个穿过模板的通道，并且在某些情况下，通道可具有小于1mm的横截面尺寸。模板可以以在基底上形成微观细胞图案的方式施加到细胞培养基底上，并且在某些情况下，模板可以与基底形成密封或其他水密接触。在一个实施例中，可以在播种之前将模板施加到基底上。将细胞施加到表面的未覆盖部分，然后如果需要可以去除模板。模板可以例如通过将弹性体前体注入模具中或通过在施加压力的同时固化弹性体前体来制造。在一些实施例中，模板的表面也可以被氧化，或者可以以使得模板的表面更亲水的方式处理，例如通过使用氧等离子体处理来处理表面。

2. 发明申请

WO2003062920A3 ELASTOMERIC STENCILS FOR MICROPATTERNING CELLS 审中-公开
公开(公告)号：WO2003062920A3
公开(公告)日：2003-07-31
申请号：PCT/US2002/026003
申请日：2002-08-15
申请人： THE GENERAL HOSPITAL CORPORATION , THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS , TONER, Mehmet , FOLCH, Albert , BEEBE, David, J.
发明人： TONER, Mehmet , FOLCH, Albert , BEEBE, David, J.
IPC分类号： C12Q1/34
摘要： The present invention provides new fabrication methods and techniques for making and using stencils to micropattern cells. These techniques may allow the fabrication of thinner stencils. The invention provides a system for selectively applying cells to predetermined regions on a substrate. A stencil is positioned adjacent to a substrate to cover some portions of the substrate, while allowing other portions of the substrate to remain uncovered. The stencil may have one or more channels passing through the stencil, and in certain cases, the channels may have a cross-sectional dimension of less than 1 mm. The stencil may be applied to a cell culture substrate in such a way as to form a microscopic cellular pattern on the substrate, and the stencil may form a seal or other watertight contact with the substrate in some cases. In one embodiment, the stencil may be applied to a substrate before seeding. Cells are applied to the uncovered portions of the surface, then the stencil may be removed if desired. The stencil may be fabricated, for example, by injecting an elastomeric precursor into a mold, or by curing an elastomeric precursor while applying pressure. In some embodiments, the surface of the stencil may also be oxidized, or it may be treated in such a way as to render the surface of the stencil more hydrophilic, for example, by treating the surface using oxygen-plasma treatment.

3. 发明申请

WO2004092403A1 MICROWELL ARRAYS WITH NANOHOLES 审中-公开
标题翻译： MICROWELL阵列与纳米胶
公开(公告)号：WO2004092403A1
公开(公告)日：2004-10-28
申请号：PCT/US2004/010373
申请日：2004-04-05
申请人： UNIVERSITY OF WASHINGTON , FOLCH, Albert , KOSAR, Turgut, Fettah
发明人： FOLCH, Albert , KOSAR, Turgut, Fettah
IPC分类号： C12Q1/24
CPC分类号： G01N33/48728 , B01L3/50255 , B01L3/5027 , B01L2200/0668 , B01L2300/0819 , B01L2400/0409 , B01L2400/0415 , B01L2400/043 , B01L2400/0487 , C12M23/16 , C12M25/04
摘要： A device for conducting parallel analysis or manipulation of multiple cells or biomolecules is disclosed. In one embodiment, the device comprises a silicon chip (10) with a microwell (60), and at least one membrane (28) suspended at the bottom opening of the microwell. The suspended portion of the membrane (28) defines a nanohole (22) that provides access to the material on the other side of the membrane (28).
摘要翻译：公开了用于进行多个细胞或生物分子的平行分析或操作的装置。在一个实施例中，该装置包括具有微孔（60）的硅芯片（10）和悬浮在微孔底部开口处的至少一个膜（28）。膜（28）的悬浮部分限定了提供对膜（28）另一侧的材料的通路的纳米孔（22）。

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