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    • 7. 发明申请
    • METHODS IN CELL CULTURES, AND RELATED INVENTIONS, EMPLOYING CERTAIN ADDITIVES
    • 细胞培养方法和相关发明,使用某些添加剂
    • WO2011018472A2
    • 2011-02-17
    • PCT/EP2010/061655
    • 2010-08-11
    • BASF SEKAUFMANN, FranzMILLER, Trine Hefsgaard
    • KAUFMANN, FranzMILLER, Trine Hefsgaard
    • C12P21/00
    • C12N1/38C12P21/02
    • A method for the manufacture of products by biotechnological methods in bacterial cell culture is disclosed as well as products obtained, the use of certain additives to the media used in the manufacture of said products in bacterial cell culture media, and the use of said additives in reducing the detrimental effects of radicals in the manufacture of the products, as well as aspects related to these invention embodiments. The manufacturing process or method comprises adding one or more radical scavenging and/or antioxidative additive preferably selected from the group consisting of sterically hindered nitroxyls, sterically hindered hydroxylamines, sterically hindered hydroxylamine salt compounds, sterically hindered amino compounds and sterically hindered N-hydrocarbyloxyamines, benzofuranone compounds, as obligatory component(s) to the medium used during biosynthesis.
    • 公开了通过生物技术在细菌细胞培养中制造产品的方法,以及获得的产品,在细菌细胞培养基中用于制造所述产品的培养基中使用某些添加剂,以及所述添加剂在 降低了在制造产品中自由基的有害影响,以及与本发明实施方案相关的方面。 制备方法或方法包括加入一种或多种自由基清除和/或抗氧化添加剂,其优选选自空间位阻硝酰基,空间位阻羟胺,空间位阻羟胺盐化合物,空间位阻氨基化合物和空间位阻N-烃氧基胺,苯并呋喃酮 作为生物合成过程中使用的培养基的强制性成分。