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1. 发明申请

WO2007146767A3 BACTERIAL GLUTAMINE SYNTHETASES AND METHODS OF USE 审中-公开
标题翻译：细菌谷氨酰胺合成酶及其使用方法
公开(公告)号：WO2007146767A3
公开(公告)日：2008-04-17
申请号：PCT/US2007070690
申请日：2007-06-08
申请人： ATHENIX CORP , DUCK NICHOLAS B , HINSON TODD K , BEILINSON VADIM , SCHOUTEN LAURA COOPER , TOMSO DANIEL JOHN
发明人： DUCK NICHOLAS B , HINSON TODD K , BEILINSON VADIM , SCHOUTEN LAURA COOPER , TOMSO DANIEL JOHN
IPC分类号： A01H5/00 , C12N9/00 , C12N15/52 , C12N15/82
CPC分类号： C12N15/8277 , C12N9/93
摘要： Compositions and methods for conferring herbicide resistance to and improving nitrogen utilization of bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to herbicidal glutamine synthetase inhibitors are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides corresponding to herbicidal glutamine synthetase inhibitor-resistant polynucleotides are provided. Additionally, polypeptides corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides comprising a variant of SEQ ID NO: 1, wherein the variant polynucleotide encodes a polypeptide that is resistant to inhibition by herbicidal glutamine synthetase inhibitor.
摘要翻译：提供了赋予除草剂抵抗和改善细菌，植物，植物细胞，组织和种子的氮利用的组合物和方法。提供了包含赋予除草谷氨酰胺合成酶抑制剂的抗性或耐受性的多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中用于在植物中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了对应于除草谷氨酰胺合成酶抑制剂抗性多核苷酸的分离的多核苷酸。另外，包括对应于多核苷酸的多肽。特别地，本发明提供了包含SEQ ID NO：1的变体的分离的多核苷酸，其中所述变体多核苷酸编码对除草谷氨酰胺合成酶抑制剂具有抑制作用的多肽。

2. 发明申请

WO2007146767A8 BACTERIAL GLUTAMINE SYNTHETASES AND METHODS OF USE 审中-公开
标题翻译：细菌谷氨酰胺合成酶及其使用方法
公开(公告)号：WO2007146767A8
公开(公告)日：2008-02-14
申请号：PCT/US2007070690
申请日：2007-06-08
申请人： ATHENIX CORP , DUCK NICHOLAS B , HINSON TODD K , BEILINSON VADIM , SCHOUTEN LAURA COOPER , TOMSO DANIEL JOHN
发明人： DUCK NICHOLAS B , HINSON TODD K , BEILINSON VADIM , SCHOUTEN LAURA COOPER , TOMSO DANIEL JOHN
IPC分类号： C12N9/00 , A01H5/00 , C12N15/52 , C12N15/82
CPC分类号： C12N15/8277 , C12N9/93
摘要： Compositions and methods for conferring herbicide resistance to and improving nitrogen utilization of bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to herbicidal glutamine synthetase inhibitors are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides corresponding to herbicidal glutamine synthetase inhibitor-resistant polynucleotides are provided. Additionally, polypeptides corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides comprising a variant of SEQ ID NO: 1, wherein the variant polynucleotide encodes a polypeptide that is resistant to inhibition by herbicidal glutamine synthetase inhibitor.
摘要翻译：提供了赋予除草剂抗性并改善细菌，植物，植物细胞，组织和种子的氮利用的组合物和方法。提供了包含赋予对除草谷氨酰胺合成酶抑制剂的抗性或耐受性的多肽的编码序列的组合物。编码序列可以用于DNA构建体或表达盒中用于在植物中转化和表达。组合物还包含转化的细菌，植物，植物细胞，组织和种子。具体而言，提供了对应于除草谷氨酰胺合成酶抑制剂抗性多核苷酸的分离的多核苷酸。另外，包含对应于多核苷酸的多肽。具体而言，本发明提供了包含SEQ ID NO：1的变体的分离的多核苷酸，其中所述变体多核苷酸编码抗除草谷氨酰胺合成酶抑制剂抑制的多肽。

3. 发明申请

WO2008100353A2 IMPROVED GRG23 EPSP SYNTHASES: COMPOSITIONS AND METHODS OF USE 审中-公开
标题翻译：改进的GRG23 EPSP合成：组合物和使用方法
公开(公告)号：WO2008100353A2
公开(公告)日：2008-08-21
申请号：PCT/US2007085164
申请日：2007-11-20
申请人： ATHENIX CORP , SCHOUTEN LAURA COOPER , PETERS CHERYL , VANDE BERG BRIAN , HINSON TODD
发明人： SCHOUTEN LAURA COOPER , PETERS CHERYL , VANDE BERG BRIAN , HINSON TODD
IPC分类号： C12N15/82
CPC分类号： C12N15/8275 , C12N9/1085 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds.. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, or 35, or the nucleotide sequence set forth in SEQ ID NO:6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, or 34.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供编码抗草甘膦或多肽的分离的多核苷酸。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了分离的多核苷酸，其含有编码SEQ ID NO：9,11,13,15,17,19,21,23,25,27,29,31,33，或35，或SEQ ID NO：6,8,10,12,14,16,18,20,22,24,26,28,30,32或34所示的核苷酸序列。

4. 发明申请

WO2009099906A3 DIRECTED EVOLUTION OF GRG31 AND GRG36 EPSP SYNTHASE ENZYMES 审中-公开
标题翻译： GRG31和GRG36 EPSP合成酶的定向演化
公开(公告)号：WO2009099906A3
公开(公告)日：2009-11-05
申请号：PCT/US2009032515
申请日：2009-01-30
申请人： ATHENIX CORP , HEINRICHS VOLKER , SCHOUTEN LAURA COOPER , VANDE BERG BRIAN
发明人： HEINRICHS VOLKER , SCHOUTEN LAURA COOPER , VANDE BERG BRIAN
IPC分类号： C12N9/10 , C12N15/82
CPC分类号： C12N15/8275 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided, particularly polypeptide variants of SEQ ID NO:2 and 4. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:7-28, or the nucleotide sequence set forth in SEQ ID NO:29 or 30.
摘要翻译：提供了赋予除草剂对细菌，植物，植物细胞，组织和种子的抗性或耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含该载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中，用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。具体而言，提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸，特别是SEQ ID NO：2和4的多肽变体。另外，包含对应于多核苷酸的氨基酸序列。具体而言，本发明提供了分离的多核苷酸，其含有编码SEQ ID NO：7-28所示氨基酸序列或SEQ ID NO：29或30所示核苷酸序列的核苷酸序列。

5. 发明申请

WO2008105905A2 METHODS AND COMPOSITIONS FOR IMPROVED ENZYME ACTIVITY IN TRANSGENIC PLANTS 审中-公开
标题翻译：提高转基因植物酶活性的方法和组合物
公开(公告)号：WO2008105905A2
公开(公告)日：2008-09-04
申请号：PCT/US2007073988
申请日：2007-07-20
申请人： ATHENIX CORP , KOZIEL MICHAEL G , SCHOUTEN LAURA COOPER , VANDE BERG BRIAN
发明人： KOZIEL MICHAEL G , SCHOUTEN LAURA COOPER , VANDE BERG BRIAN
IPC分类号： A01H1/00 , A01H5/00
CPC分类号： C12N15/8275
摘要： Compositions and methods for increasing enzyme activity across a broad physiological spectrum in plants, plant cells, tissues and seeds are provided. Compositions include plants or plant parts comprising two or more polynucleotides encoding polypeptides that are active across a broader physiological spectrum than when either polynucleotide is expressed alone. Vectors comprising these polynucleotide molecules as well as host cells comprising the vectors are further provided. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In addition, methods are provided for producing the plants, plant cells, tissues and seeds of the invention. Methods for increasing plant yield and methods for conferring resistance to an herbicide in a plant are further provided.
摘要翻译：提供了用于在植物，植物细胞，组织和种子中的广泛生理谱上增加酶活性的组合物和方法。组合物包括植物或植物部分，所述植物或植物部分包含两种或更多种编码多肽的多核苷酸，所述多核苷酸在多核苷酸单独表达时比在更广泛的生理光谱中有活性进一步提供了包含这些多核苷酸分子的载体以及包含该载体的宿主细胞。组合物还包含转化的细菌，植物，植物细胞，组织和种子。另外，提供了用于生产本发明的植物，植物细胞，组织和种子的方法。还提供了增加植物产量的方法和赋予植物对除草剂抗性的方法。

6. 发明申请

WO2007103768A2 METHODS AND COMPOSITIONS FOR IMPROVED ENZYME ACTIVITY IN TRANSGENIC PLANT 审中-公开
标题翻译：在转基因植物中改善酶活性的方法和组合物
公开(公告)号：WO2007103768A2
公开(公告)日：2007-09-13
申请号：PCT/US2007063132
申请日：2007-03-02
申请人： ATHENIX CORP , KOZIEL MICHAEL G , SCHOUTEN LAURA COOPER , VANDE BERG BRIAN
发明人： KOZIEL MICHAEL G , SCHOUTEN LAURA COOPER , VANDE BERG BRIAN
IPC分类号： A01H5/00
CPC分类号： C12N15/8261 , C12N15/8274 , C12N15/8275 , Y02A40/146
摘要： Compositions and methods for increasing enzyme activity across a broad physiological spectrum in plants, plant cells, tissues and seeds are provided. Compositions include plants or plant parts comprising two or more polynucleotides encoding polypeptides that are active across a broader physiological spectrum than when either polynucleotide is expressed alone. Vectors comprising these polynucleotide molecules as well as host cells comprising the vectors are further provided. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In addition, methods are provided for producing the plants, plant cells, tissues and seeds of the invention. Methods for increasing plant yield and methods for conferring resistance to an herbicide in a plant are further provided.
摘要翻译：提供了用于增加植物，植物细胞，组织和种子中广泛生理光谱的酶活性的组合物和方法。组合物包括植物或植物部分，其包含两个或更多个编码与更多生理光谱相关的多肽的多核苷酸，而不是单个表达。还提供了包含这些多核苷酸分子的载体以及包含载体的宿主细胞。组合物还包括转化的细菌，植物，植物细胞，组织和种子。此外，提供了用于制备本发明的植物，植物细胞，组织和种子的方法。还提供了增加植物产量的方法和赋予植物中除草剂抗性的方法。

7. 发明申请

WO2007064828A3 GRG23 AND GRG51 GENES CONFERRING HERBICIDE RESISTANCE 审中-公开
标题翻译： GRG23和GRG51基因对抗药性的抵抗
公开(公告)号：WO2007064828A3
公开(公告)日：2007-08-02
申请号：PCT/US2006045908
申请日：2006-12-01
申请人： ATHENIX CORP , PETERS CHERYL L , HINSON JILL , HAMMER PHILIP E , VANDE BERG BRIAN , SCHOUTEN LAURA COOPER , CARR BRIAN
发明人： PETERS CHERYL L , HINSON JILL , HAMMER PHILIP E , VANDE BERG BRIAN , SCHOUTEN LAURA COOPER , CARR BRIAN
IPC分类号： C12N15/54 , A01H5/00 , C12N5/10 , C12N9/10 , C12N15/82 , C12Q1/48
CPC分类号： C12N15/8275 , C07K14/195 , C07K14/415 , C12N15/8274 , C12Q1/42
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds.. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID N0:2, 4, or 6, or the nucleotide sequence set forth in SEQ ID NO: 1 , 3, or 5. The present invention additionally provides a method to measure enzyme kinetic activity using fluorogenic substrates.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码抗草甘膦或多肽的分离的多核苷酸。另外，包含对应于多核苷酸的氨基酸序列。特别地，本发明提供了包含编码SEQ ID NO：2,4或6所示氨基酸序列或SEQ ID NO：1,3或5所示核苷酸序列的核苷酸序列的分离多核苷酸。本发明另外提供了使用荧光底物测量酶动力学活性的方法。

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