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1. 发明申请

WO2003055923A1 NOVEL MONOMERS USEFUL IN THE PREPARATION OF SEPARATION MATRICES 审中-公开
标题翻译：新颖的单体在分离矩阵的准备中有用
公开(公告)号：WO2003055923A1
公开(公告)日：2003-07-10
申请号：PCT/SE2002/002351
申请日：2002-12-17
申请人： AMERSHAM BIOSCIENCES AB , ALGOTSSON, Mattias , BJELLQVIST, Bengt , PALMGREN, Ronnie
发明人： ALGOTSSON, Mattias , BJELLQVIST, Bengt , PALMGREN, Ronnie
IPC分类号： C08F20/56
CPC分类号： C07C233/20 , C08F20/56 , G01N27/44747
摘要： The invention relates to an acrylic monomer defined by formula (I) wherein R 1 , R 2 and R 3 are H or CH 3 ; R 4 and R 5 are H or OH; R 6 and R 7 are H or OH; R 8 is H or CH 3 ;m is 2-4; andn and p are 0 or 1. The invention also relates to a polymer comprised of repeated such monomer units as well as a separation matrix prepared thereof.
摘要翻译：本发明涉及由式（I）定义的丙烯酸类单体，其中R 1，R 2，R 3和R 3均为H或CH 3， 3 ; R 4和R 5分别是H或OH; R 6和R 7是H或OH; R 8是H或CH 3或-Sb; m是2-4; andn和p为0或1.本发明还涉及由重复的这种单体单元组成的聚合物以及其制备的分离基质。

2. 发明申请

WO2003006979A3 MODULAR GEL-STRIP CARRIER ELEMENT 审中-公开
公开(公告)号：WO2003006979A3
公开(公告)日：2003-01-23
申请号：PCT/EP2002/007626
申请日：2002-07-09
申请人： AMERSHAM BIOSCIENCES AB , SALVEN, Owe , OLSSON, Ingmar , BJELLQVIST, Bengt
发明人： SALVEN, Owe , OLSSON, Ingmar , BJELLQVIST, Bengt
IPC分类号： G01N27/447
摘要： A carrier module for holding a gel-strip in heat transferring contact with the surface of a cooling plate or a Peltier plate in an electrophoretic separation process, comprising a carrier member (1,10) formed in a plastic material for disposable use; the carrier member having a top plane formed with at least one elongate channel (2) for receiving the gel-strip in longitudinal contact with a bottom portion (5) of the channel, the carrier member being molded to have a shell-formed structure wherein said channel is depressed from the top plane of the shell structure so as to be able to rest with its bottom in contact with the cooling plate surface.

3. 发明申请

WO2006036119A1 METHOD AND DEVICE FOR ISOELECTRIC FOCUSING 审中-公开
标题翻译：用于ISOELECTRIC聚焦的方法和装置
公开(公告)号：WO2006036119A1
公开(公告)日：2006-04-06
申请号：PCT/SE2005/001427
申请日：2005-09-28
申请人： AMERSHAM BIOSCIENCES AB , BJELLQVIST, Bengt
发明人： BJELLQVIST, Bengt
IPC分类号： G01N27/447
CPC分类号： B01D57/02 , C07K1/28 , G01N27/44795
摘要： The present invention relates to the field of electrophoresis and more specifically to a method and device of separating protein and/or peptide components by isoelectric focusing by arranging the gels or strips, preferably IPG (immobilised pH gradient) gel(s) or strip(s), in a novel way during focusing. The gels or strips are arranged with the width of the gels in substantially vertical direction. This arrangement occupies a minimum of space for long iPG gels and avoids liquid spreading on the gel during long focusing times. IEF run in the device of the invention gives high protein resolution and enables analysis of low abundant proteins.
摘要翻译：本发明涉及电泳领域，更具体地涉及通过等离子体聚焦来分离蛋白质和/或肽组分的方法和装置，所述方法和装置优选配置凝胶或条带，优选IPG（固定化pH梯度）凝胶或条带），以一种新颖的方式聚焦。凝胶或条带以凝胶的宽度基本上垂直的方向排列。这种布置在长iPG凝胶中占用最小的空间，并避免在长时间聚焦时在胶上液体扩散。在本发明的装置中运行的IEF提供高蛋白质分辨率并能够分析低丰度蛋白质。

4. 发明申请

WO2011065909A1 PRINTING OF ELECTROPHORESIS GELS 审中-公开
标题翻译：印刷电泳胶
公开(公告)号：WO2011065909A1
公开(公告)日：2011-06-03
申请号：PCT/SE2010/051306
申请日：2010-11-26
申请人： GE HEALTHCARE BIO-SCIENCES AB , BJELLQVIST, Bengt , BJERNELD, Erik , JOHANSSON, Jan-Olof , PALMGREN, Ronnie
发明人： BJELLQVIST, Bengt , BJERNELD, Erik , JOHANSSON, Jan-Olof , PALMGREN, Ronnie
IPC分类号： G01N27/447 , B01J20/291
CPC分类号： G01N27/44747 , C07K1/26 , C08F2/46
摘要： The invention relates to a method to produce gels for electrophoresis, wherein at least two monomer solutions are printed in a pattern on a substrate and the printed substrate is exposed to electromagnetic or ionising radiation so as to initiate polymerisation. The gels are useful for electrophoretic separation of proteins, peptides and/or nucleic acids.
摘要翻译：本发明涉及一种生产电泳用凝胶的方法，其中至少两种单体溶液以衬底上的图案印刷，并将印刷的基材暴露于电磁辐射或电离辐射以引发聚合反应。凝胶可用于蛋白质，肽和/或核酸的电泳分离。

5. 发明申请

WO2005062032A1 NOVEL USE OF POSITIVELY CHARGED SUPPORT 审中-公开
标题翻译：使用积极支持的新功能
公开(公告)号：WO2005062032A1
公开(公告)日：2005-07-07
申请号：PCT/SE2004/001872
申请日：2004-12-15
申请人： AMERSHAM BIOSCIENCES AB , BJELLQVIST, Bengt , BUSSON, Philippe , OLSSON, Ingmar
发明人： BJELLQVIST, Bengt , BUSSON, Philippe , OLSSON, Ingmar
IPC分类号： G01N27/447
CPC分类号： G01N27/44743 , B01D57/02 , C07K1/18
摘要： The present invention relates to a novel use of a positively charged support, namely as a sample loading support for loading samples onto an IPG (Immobilised pH gradient) gel. The support or paper is provided with positively charged groups, such as cation groups, and is used to load samples from the cathode side of the IPG gel. Furthermore, the invention relates to a kit comprising a positively charged sample application support as above and an IPG gel, preferably a pre-swollen RTG gel comprising an acidic pH-interval.
摘要翻译：本发明涉及带正电荷的载体的新用途，即将样品加载到IPG（固定化pH梯度）凝胶上的样品负载载体。支撑体或纸张具有带正电荷的基团，例如阳离子基团，并用于从IPG凝胶的阴极侧加载样品。此外，本发明涉及一种试剂盒，其包含如上所述的带正电荷的样品施用载体和IPG凝胶，优选包含酸性pH间隔的预溶胀RTG凝胶。

6. 发明申请

WO2006006924A1 METHOD AND KIT FOR PEPTIDE ANALYSIS 审中-公开
标题翻译：肽分析方法和试剂盒
公开(公告)号：WO2006006924A1
公开(公告)日：2006-01-19
申请号：PCT/SE2005/001113
申请日：2005-07-05
申请人： AMERSHAM BIOSCIENCES AB , BJELLQVIST, Bengt , FENYÖ, David , HEDBERG, Jesper , NEU, Henrik
发明人： BJELLQVIST, Bengt , FENYÖ, David , HEDBERG, Jesper , NEU, Henrik
IPC分类号： G01N33/68
CPC分类号： G01N33/6848
摘要： The present invention relates to a method for peptide analysis, comprising the following steps: a) tagging N- terminals of peptides in sample(s) with mass tagging reagent(s) and mass balancing C-terminals of said peptides with mass balancing reagent(s), or vice versa; and b) mass spectrometry analysis of said peptides. The present invention also relates to a kit with global mass tagging reagents and mass balancing reagents for use in said method and a database with specific peptide information.
摘要翻译：本发明涉及一种肽分析方法，包括以下步骤：a）用质量标签试剂和所述肽的质量平衡C-末端用质量平衡试剂标记样品中的肽的N-末端（ s），反之亦然; 和b）所述肽的质谱分析。本发明还涉及具有全球质量标签试剂和用于所述方法的质量平衡试剂盒和具有特定肽信息的数据库的试剂盒。

7. 发明申请

WO2007089188A1 METHOD, COMPOSTION AND KIT FOR ISOELECTRIC FOCUSING 审中-公开
标题翻译：用于ISOELECTRIC聚焦的方法，组合和工具包
公开(公告)号：WO2007089188A1
公开(公告)日：2007-08-09
申请号：PCT/SE2007/000078
申请日：2007-01-30
申请人： GE HEALTHCARE BIO-SCIENCES AB , BJELLQVIST, Bengt , NEU, Henrik , UHLÉN, Kristina
发明人： BJELLQVIST, Bengt , NEU, Henrik , UHLÉN, Kristina
IPC分类号： G01N27/447 , C08F220/00
CPC分类号： G01N27/44795
摘要： The present invention relates to a method, composition and a kit for isoelectric focusing. More closely, the present invention involves the use of to modified carrier ampholytes which are easy to separate from the proteins/peptides following finished focusing. The modification is that the carrier ampholytes are derivatised with handles interacting with a solid phase/matrix which makes them easy to remove and separate from the peptides after finished focusing. In this way, there is very little or no background from carrier ampholytes in the following MS-spectra.
摘要翻译：本发明涉及等电聚焦的方法，组合物和试剂盒。更严格地说，本发明涉及使用经修饰的载体两性电解质，其在完成聚焦之后容易与蛋白质/肽分离。修饰是载体两性电解质用与固相/基质相互作用的手段衍生，这使得它们在完成聚焦之后易于从肽中除去和分离。以这种方式，在以下MS谱中载体两性电解质的背景很少或没有。

8. 发明申请

WO2008039128A1 USE OF AN ELECTROPHORETIC GEL PROVIDED WITH A NON-ADHERENT POLYMER FILM 审中-公开
标题翻译：使用非附着聚合物薄膜提供的电泳胶
公开(公告)号：WO2008039128A1
公开(公告)日：2008-04-03
申请号：PCT/SE2007/000837
申请日：2007-09-24
申请人： GE HEALTHCARE BIO-SCIENCES AB , BJELLQVIST, Bengt , EDLUND, Sofia , HEDBERG, Jesper
发明人： BJELLQVIST, Bengt , EDLUND, Sofia , HEDBERG, Jesper
IPC分类号： G01N27/447 , B01D57/02
CPC分类号： G01N27/44739
摘要： This invention relates to electrophoresis, in particular pre-cast mini gels with a low fluorescent polymer backing that is not adherent to the gel. Use of this type of small gel simplifies blotting procedures, such as Southern and Western blotting, after electrophoresis.
摘要翻译：本发明涉及电泳，特别是具有不与凝胶粘附的低荧光聚合物背衬的预制微型凝胶。使用这种类型的小凝胶简化了印迹方法，如电泳后的Southern和Western印迹。

9. 发明申请

WO2007126354A1 ELECTRODIC BRIDGE 审中-公开
标题翻译：电极桥
公开(公告)号：WO2007126354A1
公开(公告)日：2007-11-08
申请号：PCT/SE2007/000378
申请日：2007-04-20
申请人： GE HEALTHCARE BIO-SCIENCES AB , BJELLQVIST, Bengt , UHLÉN, Kristina
发明人： BJELLQVIST, Bengt , UHLÉN, Kristina
IPC分类号： G01N27/447 , B01D57/02 , C07K1/26
CPC分类号： C07K1/28 , B01D57/02 , G01N27/44795
摘要： The present invention relates to an electrode bridge or sample application bridge for use in electrophoresis, preferably isoelectric focusing, IEF. More closely, the invention relates to an electrodic bridge or sample loading bridge for preventing denaturant depletion from the IPG (immobilised pH gradient) gel and, optionally, for loading samples onto the IPG gel. The pH of the bridge is adjustable for adoption to positioning at the acid as well as basic end of the IPG strips.
摘要翻译：本发明涉及用于电泳，优选等电聚焦IEF的电极桥或样品施加桥。更详细地说，本发明涉及一种用于防止来自IPG（固定化pH梯度）凝胶的变性剂消耗以及任选地将样品加载到IPG凝胶上的电桥或样品加载桥。桥梁的pH值可调节，用于在酸性和IPG条的基端部定位。

10. 发明申请

WO2009014477A1 ISOELECTRIC POINT MARKERS 审中-公开
公开(公告)号：WO2009014477A1
公开(公告)日：2009-01-29
申请号：PCT/SE2008/000449
申请日：2008-07-14
申请人： GE HEALTHCARE BIO-SCIENCES AB , BJELLQVIST, Bengt , BURROWS, Miles, W.
发明人： BJELLQVIST, Bengt , BURROWS, Miles, W.
IPC分类号： C07K1/13 , C07K1/28 , G01N27/447 , C09B23/08
CPC分类号： G01N27/44795 , C07K1/285 , C07K7/06 , C07K7/08 , C07K14/395 , C09B23/08 , G01N27/44726
摘要： The present invention relates to the field of isoelectric focussing or 2D electrophoresis, in particular isoelectric point markers with fluorescence detection. The marker comprises an oligopeptide covalently labelled with a fluorescent dye and is characterised in that said dye has a net charge that will maintain the overall net charge of the oligopeptide upon being bound to said oligopeptide. The invention also relates to an electrophoretic method for analysis of proteins using these markers.
摘要翻译：本发明涉及等电聚焦或2D电泳领域，特别是具有荧光检测的等电点标记。标记物包含用荧光染料共价标记的寡肽，其特征在于所述染料具有净电荷，其将在结合所述寡肽时保持寡肽的总净电荷。本发明还涉及使用这些标记物分析蛋白质的电泳方法。

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