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1. 发明申请

WO03089579A2 INCORPORATION OF ANAEROBIC BACTERIA IN FEED FORMULATION 审中-公开
标题翻译：饲料配方中厌氧细菌的并入
公开(公告)号：WO03089579A2
公开(公告)日：2003-10-30
申请号：PCT/US0311366
申请日：2003-04-14
申请人： ALLNUTT THOMAS F C , HAREL MOTI
发明人： ALLNUTT THOMAS F C , HAREL MOTI
IPC分类号： A23K1/00 , A23K1/18 , C12N
CPC分类号： A23K50/80 , A23K10/16 , A23K10/18 , A23K50/75
摘要： Anaerobic bacteria are incorporated in a feed such that they provide a benefit to the consuming animal. Anaerobic bacteria can be viable and colonize the gut to provide this benefit by displacing harmful bacteria, secreting a particular agent (e.g., enzyme, antibiotic or bioactive compound), binding or sequestering harmful organisms or compounds, or providing a beneficial physical effect. Anaerobic bacteria can also be added n a non-viable form wherein the added bacteria provide a benefit to the consuming organism by delivering preformed compounds such as enzymes, bioactive agents, or polymers. Recombinant anaerobes can be utilized for any of the above purposes. Feeds can contain naked bacteria, spores, treated bacteria such as encapsulated, coated or freeze dried bacteria, in order to maintain either viability or stability of the active function.
摘要翻译：厌氧细菌被掺入饲料中，以便它们为消费动物提供益处。通过置换有害细菌，分泌特定试剂（例如酶，抗生素或生物活性化合物），结合或隔离有害生物体或化合物，或提供有益的物理效应，厌氧细菌可以存活并殖民肠道以提供此益处。厌氧细菌也可以以不存活的形式加入，其中添加的细菌通过递送预形成的化合物例如酶，生物活性剂或聚合物而为消费生物提供益处。重组厌氧菌可用于上述任何目的。饲料可含有裸露的细菌，孢子，经处理的细菌，如包封的，包衣的或冷冻干燥的细菌，以维持活性功能的活力或稳定性。

2. 发明申请

WO2008140610A9 ANTIGENICITY OF INFECTIOUS PANCREATIC NECROSIS VIRUS VP2 SUB-VIRAL PARTICLES EXPRESSED IN YEAST 审中-公开
标题翻译：感染性胰腺炎病毒VP2亚历山大小肠病毒的抗生素
公开(公告)号：WO2008140610A9
公开(公告)日：2009-04-16
申请号：PCT/US2007087942
申请日：2007-12-18
申请人： ADVANCED BIONUTRITION CORP , DHAR ARUN K , BOWERS ROBERT M , ALLNUTT THOMAS F C
发明人： DHAR ARUN K , BOWERS ROBERT M , ALLNUTT THOMAS F C
IPC分类号： C12N15/40 , A61K39/12 , C12N7/04
CPC分类号： A61K39/12 , A61K2039/51 , A61K2039/5258 , A61K2039/542 , A61K2039/552 , C07K14/005 , C12N7/00 , C12N2720/10022 , C12N2720/10023 , C12N2720/10034

3. 发明申请

WO0181603A3 TROPHIC CONVERSION OF OBLIGATE PHOTOTROPHIC ALGAE THROUGH METABOLIC ENGINEERING 审中-公开
标题翻译：通过代谢工程进行的白藜芦醇的热转化
公开(公告)号：WO0181603A3
公开(公告)日：2002-05-23
申请号：PCT/US0112789
申请日：2001-04-20
申请人： MARTEK BIOSCIENCES CORP , APT KIRK E , ALLNUTT THOMAS F C , KYLE DAVID J , LIPPMEIER JAMES C
发明人： APT KIRK E , ALLNUTT THOMAS F C , KYLE DAVID J , LIPPMEIER JAMES C
IPC分类号： C12N15/09 , C12N1/12 , C12N1/13 , C12N15/65 , C12N15/79 , C12N15/82 , C12Q1/02 , C07K14/415 , C07K14/47
CPC分类号： C12N15/8209 , C12N1/12 , C12N15/65 , C12N15/79 , C12N15/8207 , Y10S435/946
摘要： Most microalgae are obligate photoautotrophs and their growth is strictly dependent on the generation of photosynthetically-derived energy. In this study it is shown that the microalga Phaeodaclylurn tricornutum can be engineered to import glucose and grow in the dark through the introduction of genes encoding glucose transporters. Both the human and Chlorella kessleri glucose transporters facilitated the uptake of glucose by P. tricornutum , allowing the cells to metabolize exogenous organic carbon and thrive, independent of light. This is the first successful trophic conversion of an obligate photoautotroph through metabolic engineering, and it demonstrates that methods of cell nourishment can be fundamentally altered with the introduction of a single gene. Since strains transformed with the glucose transport genes are able to grow non-photosynthetically, they can be exploited for the analysis of photosynthetic processes through mutant generation and characterization. Finally, this work also represents critical progress toward large-scale commercial exploitation of obligate phototrophic algae through the use of microbial fermentation technology, eliminating significant limitations resulting from light-dependent growth.
摘要翻译：大多数微藻是专性光自养体，其生长严格依赖于光合作用衍生能量的产生。在本研究中，显示微藻Phaeodaclylurn tricornutum可以通过引入编码葡萄糖转运蛋白的基因进行工程化以导入葡萄糖并在黑暗中生长。人和小球藻凯斯勒葡萄糖转运蛋白均促进了P的葡萄糖摄取。三角褐斑，允许细胞代谢外源有机碳，并且独立于光而茁壮成长。这是通过代谢工程首次成功地营养专一性自养型营养转化，并且表明通过引入单一基因可以从根本上改变细胞营养的方法。由于用葡萄糖转运基因转化的菌株能够非光合作用生长，因此可以通过突变体的产生和表征来开发光合作用的分析。最后，这项工作也是通过使用微生物发酵技术大规模商业开发专用光营养藻类的重要进展，消除了光依赖性生长造成的重大限制。

4. 发明申请

WO2005102041A3 CRUSTACEAN EXPRESSION VECTOR 审中-公开
标题翻译：表达式表达向量
公开(公告)号：WO2005102041A3
公开(公告)日：2006-03-30
申请号：PCT/US2005012979
申请日：2005-04-15
申请人： ADVANCED BIONUTRITION CORP , DHAR ARUN K , ALLNUTT THOMAS F C
发明人： DHAR ARUN K , ALLNUTT THOMAS F C
IPC分类号： A01K67/033 , C12N15/85 , C12N15/00 , C12N15/09 , C12N15/63 , C12N15/70 , C12N15/74 , C12N15/87 , C12P21/06
CPC分类号： A01K67/0338 , A01K2217/05 , A01K2227/70 , A01K2267/02 , A01K2267/0393 , C12N15/85 , C12N15/8509 , C12N2800/90 , C12N2830/60 , C12N2840/203
摘要： Methods and constructs for genetic manipulation of one or more of shrimp, shellfish, mollusks, and fish are disclosed. The nucleic acid construct includes a promoter and an internal ribosome entry site of an insect picornavirus, such as a cricket paralysis-like picornavirus. One or more open reading frames can be operably associated with one or both of the promoter and the internal ribosome entry site, and one or more proteins or protein subunits can be expressed upon introduction of the construct into a host cell, such as into a shrimp. Method for producing immortalized crustacean cell lines using enhancer elements derived from shrimp and/or shrimp viruses are also described.
摘要翻译：公开了一种或多种虾，贝类，软体动物和鱼的遗传操作的方法和构造。核酸构建体包括昆虫小核糖核酸病毒（例如板球麻痹样小核糖核酸病毒）的启动子和内部核糖体进入位点。一个或多个开放阅读框可以与启动子和内部核糖体进入位点中的一个或两个可操作地相关联，并且一个或多个蛋白质或蛋白质亚基可以在将构建体引入宿主细胞时表达，例如进入虾。还描述了使用源自虾和/或虾病毒的增强子元件生产永生化甲壳类细胞系的方法。

5. 发明申请

WO2005102041B1 CRUSTACEAN EXPRESSION VECTOR 审中-公开
标题翻译： CRUSTACEAN表达载体
公开(公告)号：WO2005102041B1
公开(公告)日：2006-11-09
申请号：PCT/US2005012979
申请日：2005-04-15
申请人： ADVANCED BIONUTRITION CORP , DHAR ARUN K , ALLNUTT THOMAS F C
发明人： DHAR ARUN K , ALLNUTT THOMAS F C
IPC分类号： A01K67/033 , C12N15/85
CPC分类号： A01K67/0338 , A01K2217/05 , A01K2227/70 , A01K2267/02 , A01K2267/0393 , C12N15/85 , C12N15/8509 , C12N2800/90 , C12N2830/60 , C12N2840/203
摘要： Methods and constructs for genetic manipulation of one or more of shrimp, shellfish, mollusks, and fish are disclosed. The nucleic acid construct includes a promoter and an internal ribosome entry site of an insect picornavirus, such as a cricket paralysis-like picornavirus. One or more open reading frames can be operably associated with one or both of the promoter and the internal ribosome entry site, and one or more proteins or protein subunits can be expressed upon introduction of the construct into a host cell, such as into a shrimp. Method for producing immortalized crustacean cell lines using enhancer elements derived from shrimp and/or shrimp viruses are also described.
摘要翻译：公开了用于对虾，贝类，软体动物和鱼中的一种或多种进行遗传操作的方法和构建体。核酸构建体包括昆虫微核糖核酸病毒的启动子和内部核糖体进入位点，如蟋蟀麻痹样小核糖核酸病毒。一个或多个开放阅读框可以与启动子和内部核糖体进入位点中的一个或两个可操作地相关联，并且可以在将构建体引入宿主细胞例如虾中时表达一个或多个蛋白质或蛋白质亚基。还描述了使用来源于虾和/或虾病毒的增强子元件生产永生化甲壳类动物细胞系的方法。

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