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1. 发明申请

WO02063271A8 ACTIVITY BASED PROBE ANALYSIS 审中-公开
标题翻译：基于活动的探索分析
公开(公告)号：WO02063271A8
公开(公告)日：2002-10-24
申请号：PCT/US0203808
申请日：2002-02-05
申请人： ACTIVX BIOSCIENCES INC , PATRICELLI MATTHEW P
发明人： PATRICELLI MATTHEW P
IPC分类号： C40B30/04 , G01N21/64 , G01N33/58 , G01N33/68 , G01N
CPC分类号： C40B30/04 , G01N21/6428 , G01N33/582 , G01N33/6842 , G01N33/6845 , Y10S436/80
摘要： Methods and compositions are described for analyzing complex protein mixtures using fluorescent activity-based probes. In particular, probes that specifically react with and bind to the active form of one or more target proteins are employed. Fluorescent signals obtained from the labeled active target proteins can be related to the presence or amount of active members of the desired target protein class. The methods and compositions described herein can be used, for example, to provide diagnostic information concerning pathogenic states, in identifying proteins that may act as therapeutic targets, and in drug discovery.
摘要翻译：描述了使用基于荧光活性的探针分析复杂蛋白质混合物的方法和组合物。特别地，使用与一种或多种靶蛋白的活性形式特异性反应并结合的探针。从标记的活性靶蛋白获得的荧光信号可以与所需靶蛋白质类的活性成员的存在或数量相关。本文描述的方法和组合物可用于例如提供关于致病状态的诊断信息，鉴定可用作治疗靶标的蛋白质，以及在药物发现中。

2. 发明申请

WO2004072103A3 MACROMOLECULE IDENTIFICATION MADE BY MASS SPECTROMETRY AND DATABASE SEARCHING 审中-公开
标题翻译：通过大量光谱和数据库搜索制作的大分子鉴定
公开(公告)号：WO2004072103A3
公开(公告)日：2006-03-30
申请号：PCT/US2004004016
申请日：2004-02-11
申请人： ACTIVX BIOSCIENCES INC , PATRICELLI MATTHEW P , ZHANG BAOHONG
发明人： PATRICELLI MATTHEW P , ZHANG BAOHONG
IPC分类号： C07K20060101 , G01N33/68 , G01N33/00 , G06F17/11 , G06F17/50
CPC分类号： G01N33/6851
摘要： A method of determining the parent ion charge state of a tandem MS spectrum. The parent ion is labeled with a probe that is cleaved as a result of the MS. A candidate parent ion charge state is chosen and the spectrum is searched for a peak having a value F, where F is the ratio between (i) the mass of the parent ion minus the mass of the cleaved probe and (ii) the difference between the candidate parent ion charge state and the charge of the cleaved probe. A method of removing a parent ion mass dependence and a parent ion charge dependence from a cross correlation score between a predicted spectrum and an experimental spectrum. A method of computing a false positive gamma distribution curve for a data set of tandem MS spectra. A method of reducing a number of false positive assignments made to tandem microcopy spectra in a tandem MS data set. In this method, the spectra are processed in a manner that is based on the presence or absence of peak fragments within the spectra.
摘要翻译：确定串联质谱的母离子电荷状态的方法。母体离子用作为MS结果切割的探针标记。选择候选母体离子电荷状态，并且搜索具有值F的峰的光谱，其中F是（i）母体离子的质量减去切割的探针的质量之间的比率，和（ii）候选母体离子电荷状态和切割探针的电荷。从预测光谱和实验光谱之间的互相关得分去除母离子质量依赖性和亲离子电荷依赖性的方法。计算串联质谱数据集的假阳性γ分布曲线的方法。一种在串联MS数据集中减少对串联显微镜光谱进行的假阳性分配的方法。在该方法中，以基于光谱内存在或不存在峰片段的方式处理光谱。

3. 发明申请

WO02063271A3 ACTIVITY BASED PROBE ANALYSIS 审中-公开
标题翻译：基于活动的探索分析
公开(公告)号：WO02063271A3
公开(公告)日：2003-07-10
申请号：PCT/US0203808
申请日：2002-02-05
申请人： ACTIVX BIOSCIENCES INC , PATRICELLI MATTHEW P
发明人： PATRICELLI MATTHEW P
IPC分类号： C40B30/04 , G01N21/64 , G01N33/58 , G01N33/68 , G01N33/533 , C07K1/22 , C07K1/36
CPC分类号： C40B30/04 , G01N21/6428 , G01N33/582 , G01N33/6842 , G01N33/6845 , Y10S436/80
摘要： Methods and compositions are described for analyzing complex protein mixtures using fluorescent activity-based probes. In particular, probes that specifically react with and bind to the active form of one or more target proteins are employed. Fluorescent signals obtained from the labeled active target proteins can be related to the presence or amount of active members of the desired target protein class. The methods and compositions described herein can be used, for example, to provide diagnostic information concerning pathogenic states, in identifying proteins that may act as therapeutic targets, and in drug discovery.
摘要翻译：描述了使用基于荧光活性的探针分析复杂蛋白质混合物的方法和组合物。特别地，使用与一种或多种靶蛋白的活性形式特异性反应并结合的探针。从标记的活性靶蛋白获得的荧光信号可以与所需靶蛋白质类的活性成员的存在或数量相关。本文描述的方法和组合物可用于例如提供关于致病状态的诊断信息，鉴定可用作治疗靶标的蛋白质以及药物发现。

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