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1. 发明申请

WO2020033162A1 SYSTEMS AND METHODS FOR SUPPORTING MULTIPLE AUTOMATED WORKFLOWS 审中-公开
公开(公告)号：WO2020033162A1
公开(公告)日：2020-02-13
申请号：PCT/US2019/043512
申请日：2019-07-25
申请人： LIFEFOUNDRY, INC. , CHAO, Ran , JIANG, Jing , MENG, Shaoyu , ZHAO, Huimin
发明人： CHAO, Ran , JIANG, Jing , MENG, Shaoyu , ZHAO, Huimin
IPC分类号： G06Q10/06 , G05B19/18
摘要： Systems and methods for automated workflow comprise assigning a set of first targets to an uncompiled first workflow. The uncompiled first workflow specifies a first set of process modules. Each such module is associated with a subset of unit operations. Each unit operation includes a time interval and specifies an instrument. For each target in the set of first targets, the uncompiled workflow is translated into an instance of a compiled first workflow comprising a linear temporal order of unit operations, each including execution instructions for an addressed instrument. A set of second targets is obtained and assigned a second uncompiled workflow. Compilation of the second uncompiled workflow for each second target produces a different instance of a compiled second workflow. Each second compiled workflow comprises a linear temporal order of unit operations, with each unit operation including execution instructions for an addressed instrument and specifying a time interval.

2. 发明申请

WO2012045088A9 COMBINATORIAL DESIGN OF HIGHLY EFFICIENT HETEROLOGOUS PATHWAYS 审中-公开
标题翻译：高效率异常路径的组合设计
公开(公告)号：WO2012045088A9
公开(公告)日：2012-07-26
申请号：PCT/US2011054650
申请日：2011-10-03
申请人： UNIV ILLINOIS , ZHAO HUIMIN , KIM BYOUNGJIN , DU JING , YUAN YONGBO , ERIKSEN DAWN , SI TONG
发明人： ZHAO HUIMIN , KIM BYOUNGJIN , DU JING , YUAN YONGBO , ERIKSEN DAWN , SI TONG
IPC分类号： C12N15/52
CPC分类号： C12P7/06 , C07K14/39 , C12N9/0006 , C12N9/1205 , C12N9/2445 , C12N15/1093 , C12N15/52 , C12N15/81 , C12P7/10 , C12Y101/01009 , C12Y101/0101 , C12Y101/01307 , C12Y207/01017 , C12Y302/01021 , Y02E50/16 , Y02E50/17
摘要： The present disclosure relates to the production of highly efficient heterologous pathways in host cells by identifying favorable enzyme and/or promoter combinations. In particular the present disclosure provides methods for assembly and selection of multi-step xylose and arabinose/xylose utilization pathways from a library of fungal enzymes. The present disclosure further provides compositions containing favorable enzyme combinations, as well as recombinant yeast expressing such combinations, and methods of use for bioconversion of pentose sugars. Also provided are compositions and methods involving favorable expression patterns identified by utilization of combinations of promoters of varying strengths. Provided herein are methods for assembly and selection of multi-step xylose, arabinose/xylose, and cellobiose utilization pathways from a library of promoters of varying strengths. The present disclosure further provides compositions containing heterologous enzyme-coding polynucleotides under the control of favorable promoters, as well as recombinant yeast expressing such enzymes, and methods of their use for bioconversion of pentose and/or hexose sugars.
摘要翻译：本公开涉及通过鉴定有利的酶和/或启动子组合在宿主细胞中产生高效的异源途径。特别地，本公开提供了从真菌酶文库中组装和选择多步木糖和阿拉伯糖/木糖利用途径的方法。本公开进一步提供含有有利的酶组合的组合物，以及表达这种组合的重组酵母以及用于戊糖的生物转化的方法。还提供了包括通过利用不同强度的启动子的组合鉴定的有利的表达模式的组合物和方法。本文提供了用于从不同强度的启动子文库中组装和选择多步木糖，阿拉伯糖/木糖和纤维二糖利用途径的方法。本公开进一步提供了含有有利启动子控制的异源酶编码多核苷酸的组合物，以及表达这些酶的重组酵母，以及它们用于戊糖和/或己糖的生物转化的方法。

3. 发明申请

WO2008131215A3 ENGINEERED PHOSPHITE DEHYDROGENASE MUTANTS 审中-公开
公开(公告)号：WO2008131215A3
公开(公告)日：2008-10-30
申请号：PCT/US2008/060814
申请日：2008-04-18
申请人： BIOTECHNOLOGY RESEARCH AND DEVELOPMENT CORPORATION , THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS , ZHAO, Huimin , MCLACHLAN, Michael , JOHANNES, Tyler
发明人： ZHAO, Huimin , MCLACHLAN, Michael , JOHANNES, Tyler
IPC分类号： C12N9/04 , C12N1/20 , C12N15/00 , C12Q1/00 , C12Q1/34 , C12Q1/68 , C12P21/06 , C12P21/04 , A61K38/44 , C07H21/04 , C07K1/00 , C07K16/00
摘要： Phosphite dehydrogenase mutant enzymes provide relaxed cofactor specificity, increased thermostability, increased activity, solubility, and expression over the wild-type enzyme. The mutant enzymes are useful for nicotinamide cofactor regeneration.

4. 发明申请

WO2006074194A3 ENGINEERED PHOSPHITE DEHYDROGENASE MUTANTS FOR NICOTINAMIDE COFACTOR REGENERATION 审中-公开
标题翻译：工程磷酸脱氢酶突变体用于烟酰胺类辅酶再生
公开(公告)号：WO2006074194A3
公开(公告)日：2006-10-19
申请号：PCT/US2006000135
申请日：2006-01-03
申请人： BIOTECHNOLOGY RES & DEV , UNIV ILLINOIS , ZHAO HUIMIN , WOODYER RYAN , METCALF WILLIAM , VAN DER DONK WILFRED A , JOHANNES TYLER
发明人： ZHAO HUIMIN , WOODYER RYAN , METCALF WILLIAM , VAN DER DONK WILFRED A , JOHANNES TYLER
IPC分类号： C07H21/04 , C12N9/00 , C12N9/02 , C12P19/30 , C12P21/02
CPC分类号： C12P19/36 , C12N9/0004 , C12N9/0036
摘要： Phosphite dehydrogenase mutant enzymes provide relaxed cofactor specificity, increased thermostability, increased activity, solubility, and expression over the wild-type enzyme. The mutant enzymes are useful for nicotinamide cofactor regeneration.
摘要翻译：亚磷酸盐脱氢酶突变酶与野生型酶相比提供了松弛的辅因子特异性，增加的热稳定性，增加的活性，溶解性和表达。突变酶可用于烟酰胺辅因子再生。

5. 发明申请

WO2018136903A1 SYSTEMS AND METHODS FOR SUPPORTING MULTIPLE AUTOMATED WORKFLOWS 审中-公开
公开(公告)号：WO2018136903A1
公开(公告)日：2018-07-26
申请号：PCT/US2018/014751
申请日：2018-01-22
申请人： LIFEFOUNDRY, INC. , CHAO, Ran , JIANG, Jing , MENG, Shaoyu , ZHAO, Huimin
发明人： CHAO, Ran , JIANG, Jing , MENG, Shaoyu , ZHAO, Huimin
IPC分类号： G06F9/38 , G06F19/24 , C04B30/02
摘要： Systems and methods for automated workflow comprise assigning a set of first targets to an uncompiled first workflow. The uncompiled first workflow specifies a first set of process modules. Each such module is associated with a subset of unit operations. Each unit operation includes a time interval and specifies an instrument. For each target in the set of first targets, the uncompiled workflow is translated into an instance of a compiled first workflow comprising a linear temporal order of unit operations, each including execution instructions for an addressed instrument. A set of second targets is obtained and assigned a second uncompiled workflow. Compilation of the second uncompiled workflow for each second target produces a different instance of a compiled second workflow. Each second compiled workflow comprises a linear temporal order of unit operations, with each unit operation including execution instructions for an addressed instrument and specifying a time interval.

6. 发明申请

WO2012100196A1 ENHANCED FERMENTATION OF CELLODEXTRINS AND β-D-GLUCOSE 审中-公开
标题翻译：增强CELLODEXTRINS和β-D-葡萄糖的发酵
公开(公告)号：WO2012100196A1
公开(公告)日：2012-07-26
申请号：PCT/US2012/022079
申请日：2012-01-20
申请人： THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS , THE REGENTS OF THE UNIVERSITY OF CALIFORNIA , DOUDNA CATE, James, H. , BEESON, William, T. , GALAZKA, Jonathan, M. , ZHAO, Huimin , LI, Sijin , JIN, Yong-Su , HA, Suk-Jin
发明人： DOUDNA CATE, James, H. , BEESON, William, T. , GALAZKA, Jonathan, M. , ZHAO, Huimin , LI, Sijin , JIN, Yong-Su , HA, Suk-Jin
IPC分类号： C07K14/195 , C07K14/37 , C12N9/00 , C12N9/90 , C12P5/00
CPC分类号： C12P19/12 , C07K14/195 , C07K14/37 , C12N9/2445 , C12N9/90 , C12P7/10 , C12P19/14 , C12P19/16 , C12P19/24 , Y02E50/16 , Y02E50/17
摘要： The present disclosure provides compositions and methods for the fermentation of cellodextrins and β-D-glucose. Host cells and recombinant polypeptides having glucose mutarotase activity are provided. Additionally, methods for improving cell growth, production of chemicals, and consumption of cellodextrins and β-D-glucose during fermentation of mixtures containing cellodextrins and β-D-glucose are provided.
摘要翻译：本公开提供了用于发酵纤维糊精和β-D-葡萄糖的组合物和方法。提供具有葡萄糖变异酶活性的宿主细胞和重组多肽。另外，提供了用于在含有纤维糊精和β-D-葡萄糖的混合物的发酵期间改善细胞生长，化学品生产和消耗纤维糊精和β-D-葡萄糖的方法。

7. 发明申请

WO2011130509A1 MUTANTS OF L-ARABINITOL 4-DEHYDROGENASE FROM NEUROSPORA CRASSA 审中-公开
标题翻译：来自神经细胞的L-阿拉伯糖醇4脱氢酶的突变体
公开(公告)号：WO2011130509A1
公开(公告)日：2011-10-20
申请号：PCT/US2011/032486
申请日：2011-04-14
申请人： THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS , ZHAO, Huimin , SULLIVAN, Ryan, P. , BAE, Brian , NAIR, Satish
发明人： ZHAO, Huimin , SULLIVAN, Ryan, P. , BAE, Brian , NAIR, Satish
IPC分类号： C12N9/06
CPC分类号： C12Y101/01012 , C12N9/0006
摘要： To elucidate the determinants of substrate and cofactor recognition on LAD, the first high-resolution X-ray crystallographic structure of an LAD was determined to a resolution of 2.6. The crystal structure of the ncLAD-NAD+ complex was successfully utilized for the engineering of variants with altered cofactor specificity towards NADP+. These results provide the framework for engineering of variants with increased activity that can overcome the bottleneck in pentose catabolism in yeast.
摘要翻译：为了阐明LAD上底物和辅因子识别的决定因素，确定LAD的第一个高分辨率X射线晶体结构为2.6的分辨率。 ncLAD-NAD +复合物的晶体结构成功地用于对NADP +具有改变的辅因子特异性的变体的工程化。这些结果提供了具有增加活性的变体工程的框架，可以克服酵母中戊糖分解代谢的瓶颈。

8. 发明申请

WO2011088302A4 PRODUCTION OF XYLITOL FROM A MIXTURE OF HEMICELLULOSIC SUGARS 审中-公开
标题翻译：由半胱氨酸糖混合物生产木糖醇
公开(公告)号：WO2011088302A4
公开(公告)日：2011-09-09
申请号：PCT/US2011021277
申请日：2011-01-14
申请人： UNIV ILLINOIS , ZUCHEM INC , ZHAO HUIMIN , NAIR NIKHIL UNNI , RACINE MICHAEL , WOODYER RYAN
发明人： ZHAO HUIMIN , NAIR NIKHIL UNNI , RACINE MICHAEL , WOODYER RYAN
IPC分类号： C12P7/18
CPC分类号： C12P7/18
摘要： Materials and methods are described to produce xylitol from a mixture of hemicellulosic sugars by several routes. Examples include either as a direct co-product of a biorefinery or ethanol facility, or as a stand-alone product produced from an agricultural or forestry biomass feedstock including using, e.g. ethanol waste streams.
摘要翻译：描述了材料和方法通过几种途径从半纤维素糖的混合物产生木糖醇。实例包括作为生物精炼厂或乙醇设施的直接联产品，或者作为由农业或林业生物质原料生产的独立产品，包括使用，例如，乙醇废物流。

9. 发明申请

WO2011011796A3 METHODS AND COMPOSITIONS FOR IMPROVING SUGAR TRANSPORT, MIXED SUGAR FERMENTATION, AND PRODUCTION OF BIOFUELS 审中-公开
公开(公告)号：WO2011011796A3
公开(公告)日：2011-09-09
申请号：PCT/US2010043279
申请日：2010-07-26
申请人： UNIV CALIFORNIA , TRUSTEES OF THE UNIVERSITY OF ILLINOS BOARD OF , BP CORP NORTH AMERICA INC , GLASS N LOUISE , TIAN CHAOGUANG , BEESON WILLIAM T , ZHAO HUIMIN , DU JING , CHOI JIN HO , DOUDNA CATE JANES H , GALAZKA JONATHAN M , HA SUK JIN , KIM SOON RIN , JIN YOUG-SU , LI SIJIN , SUN JIANPING , YANG XIAOMIN
发明人： GLASS N LOUISE , TIAN CHAOGUANG , BEESON WILLIAM T , ZHAO HUIMIN , DU JING , CHOI JIN HO , DOUDNA CATE JANES H , GALAZKA JONATHAN M , HA SUK JIN , KIM SOON RIN , JIN YOUG-SU , LI SIJIN , SUN JIANPING , YANG XIAOMIN
IPC分类号： C12N15/87 , C12N15/52 , C12N15/62 , C12P19/00 , C12R1/865
CPC分类号： C12P21/02 , C07K14/37 , C07K14/705 , C12N15/80 , C12N15/81 , Y02E50/16 , Y02E50/17
摘要： The present disclosure relates to host cells containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports cellodextrin into the cell. The present disclosure further relates to methods of increasing transport of cellodextrin into a cell, methods of increasing growth of a cell on a medium containing cellodextrin, methods of co-fermenting cellulose-derived and hemicellulose- derived sugars, and methods of making hydrocarbons or hydrocarbon derivatives by providing a host cell containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports cellodextrin into the cell. The present disclosure relates to host cells containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports a pentose into the cell, methods of increasing transport of a pentose into a cell, methods of increasing growth of a cell on a medium containing pentose sugars, and methods of making hydrocarbons or hydrocarbon derivatives by providing a host cell containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports a pentose into the cell.

10. 发明申请

WO2010084171A1 METHOD FOR TEMPORALLY CONTROLLING THE BIOLOGICAL ACTIVITY OF PROTEINS IN VERTEBRATES, AND APPLICATIONS THEREOF 审中-公开
标题翻译：温度控制蛋白质生物活性的方法及其应用
公开(公告)号：WO2010084171A1
公开(公告)日：2010-07-29
申请号：PCT/EP2010/050723
申请日：2010-01-22
申请人： CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS) , THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS , UNIVERSITE DE STRASBOURG , METZGER, Daniel , CHAMBON, Pierre , ZHAO, Huimin , KATZENELLENBOGEN, John
发明人： METZGER, Daniel , CHAMBON, Pierre , ZHAO, Huimin , KATZENELLENBOGEN, John
IPC分类号： C07K14/72 , C12N9/00 , C12N15/90 , C12N9/90
CPC分类号： C07K14/721 , C07K2319/715 , C12N9/90
摘要： The present invention relates to a method for tightly temporally controlling the biological activity of a protein of interest in a vertebrate, upon induction of the activity of a fusion protein comprising said protein of interest and an ERM polypeptide containing a mutated ligand binding domain of the human oestrogen receptor α, with a synthetic ligand that does not interfere with oestrogen signalling. In particular, the present invention concerns a method for generating tightly temporally-controlled targeted somatic mutations in a vertebrate, preferably a mouse, by inducing the activity of a fusion protein comprising a site- specific recombinase protein and an ERM polypeptide, with a synthetic ligand devoid of oestrogenic and anti-oestrogenic activities.
摘要翻译：本发明涉及在诱导包含所述目的蛋白质的融合蛋白的活性和含有人的突变配体结合结构域的ERM多肽时紧密地暂时地控制脊椎动物中感兴趣的蛋白质的生物学活性的方法雌激素受体α与不影响雌激素信号传导的合成配体。特别地，本发明涉及通过用合成配体诱导包含位点特异性重组酶蛋白和ERM多肽的融合蛋白的活性，在脊椎动物，优选小鼠中产生紧密的时间控制的目标体细胞突变的方法没有雌激素和抗雌激素活性。

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