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    • 8. 发明申请
    • COLOR DYEING SYSTEM FOR PLASTIC FILMS
    • 塑料膜彩色染色系统
    • WO2003054287A2
    • 2003-07-03
    • PCT/EP2002/014330
    • 2002-12-13
    • N.V. BEKAERT S.A.YANG, XiaomingTURNER, Raymond, B.
    • YANG, XiaomingTURNER, Raymond, B.
    • D06P1/651
    • D06P1/65118D06P3/52D06P5/2016Y10S8/922
    • A process for the continuous dyeing of plastic films, such as polyethylene terephthalate, is characterized by passing the film through a dye bath comprised of dye stuffs dissolved, suspended or dispersed in a liquid carrier comprised at least in part of a polymer, preferably a polyol, having at least one free hydroxyl group and a molecular weight within the range of from about 200 to about 600. The hydrogen bonding provided by the hydroxyl group, together with the high molecular weight and high boiling point of the polyol, mitigate against migration of the dye stuff out of the film, i.e., fading of the film, and impart long lasting color fastness to the film. Rapid diffusion of the dye bath into the film is enhanced by thermal and ultrasonic energization of the bath.
    • 用于连续染色塑料膜的方法,例如聚对苯二甲酸乙二醇酯,其特征在于使膜通过染料浴,染料浴由溶解,悬浮或分散在至少部分聚合物,优选多元醇 具有至少一个游离羟基并且分子量在约200至约600的范围内。由羟基提供的氢键以及多元醇的高分子量和高沸点减轻了 电影中的染料,即胶片的褪色,并赋予胶片持久的色牢度。 通过浴的热和超声波通电,可以使染浴快速扩散到膜中。
    • 9. 发明申请
    • A HIGH YIELD PERTUSSIS VACCINE PRODUCTION STRAIN AND METHOD FOR MAKING SAME
    • 高产量PERTUSSIS VACCINE生产菌株及其制备方法
    • WO0200895A2
    • 2002-01-03
    • PCT/US0120356
    • 2001-06-26
    • US HEALTHMERKEL TOD JKEITH JERRY MYANG XIAOMING
    • MERKEL TOD JKEITH JERRY MYANG XIAOMING
    • C07K14/235C12N1/21C12N15/74C12N15/63
    • C12N1/20C07K14/235C12N15/74
    • The present invention provides a vaccine production strain of Bordetella bronchiseptica that produces a pertussis toxin in high yield. The present invention provides a method for creating a Bordetella bronchiseptica cell line which produces a Bordetella pertussis toxin comprising the steps of introducing a plasmid containing a DNA encoding antibiotic resistance into a Bordetella bronchiseptica strain, selecting for isolates in which the DNA encoding antibiotic resistance is recombinantly incorporated into the chromosome in place of the Bordetella bronchiseptica toxin gene, introducing a plasmid containing DNA encoding subunits of the Bordetella pertussis toxins into the Bordetella bronchiseptica isolates; and, selecting for isolates in which DNA encoding Bordetella pertussis toxin subunit is recombinantly incorporated into the chromosome, the resulting cells producing the Bordetella pertussis toxin. The present invention further provides a method for creating a Bordetella bronchiseptica cell line which produces a Bordetella pertussis toxin and does not express filamentous hemagglutinin.
    • 本发明提供了以高产率产生百日咳毒素的支气管炎博德特氏菌疫苗生产菌株。 本发明提供了一种产生支气管炎博德特氏菌细胞系的方法,该细胞系产生百日咳博德特氏菌毒素,其包括将含有编码抗生素抗性的DNA的质粒导入< >博德特氏菌支气管炎菌株,选择其中编码抗生素抗性的DNA重组掺入染色体中以代替支气管炎博德特菌毒素基因的分离物,引入含有编码 博德特氏菌百日咳杆菌毒素进入支气管炎博德特氏菌分离株; 并且选择其中编码百日咳博德特氏菌毒素亚基的DNA重组并入染色体的分离物,所得的细胞产生百日咳博德特氏菌毒素。 本发明还提供了一种产生支气管炎博德特氏菌细胞系的方法,该细胞系产生百日咳博德特氏菌毒素并且不表达丝状血凝素。