会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 4. 发明申请
    • MODIFIED THROMBOXANE A2 RECEPTOR PROMOTER SEQUENCE
    • 改性的噻唑烷酮A2受体促进剂序列
    • WO2010017962A1
    • 2010-02-18
    • PCT/EP2009/005822
    • 2009-08-11
    • UNIVERSITY COLLEGE DUBLIN, NATIONAL UNIVERSITY OF IRELAND, DUBLINKINSELLA, ThereseGANNON, AnneMarieTURNER, ElizabethKEATING, Garrett
    • KINSELLA, ThereseGANNON, AnneMarieTURNER, ElizabethKEATING, Garrett
    • C12N15/63A61K48/00C12N5/16
    • C07K14/705C12N15/85C12N2830/002G01N2800/32G01N2800/347
    • The invention provides nucleic acid sequences useful in regulating the transcription of a gene. In particular, the invention relates to a promoter sequence, and variants thereof, that can be used to differentially regulate the transcription of a gene. The present invention, accordingly, provides methods for regulating transcription of a gene, the method comprising providing a gene transcription- regulating polynucleotide in operable association with the gene, optionally within a host cell, wherein the gene transcription-regulating polynucleotide comprises the nucleic acid sequence of SEQ ID NO:1 of the nucleic acid sequence of thromboxane A2 receptor promoter or a fragment thereof, the gene transcription-regulating polynucleotide or the fragment thereof further comprising at least one nucleic acid modification and/or substitution. The nucleic acid sequences and probes of the present invention also find utility as predictive markers for alterations in gene transcription in disease settings; or can be used to achieve over-expression of recombinant proteins in mammalian cells. Accordingly, the present invention also provides recombinant expression vectors and host cells for use in regulating the transcription of a gene.
    • 本发明提供了可用于调节基因转录的核酸序列。 特别地,本发明涉及可用于差异调节基因转录的启动子序列及其变体。 因此,本发明提供了调节基因转录的方法,所述方法包括提供可任选地在宿主细胞内与所述基因可操作关联的基因转录调节多核苷酸,其中所述基因转录调节多核苷酸包含所述核酸序列 SEQ ID NO:1的凝血恶烷A2受体启动子或其片段的核酸序列,所述基因转录调节多核苷酸或其片段还包含至少一个核酸修饰和/或取代。 本发明的核酸序列和探针也可用作疾病环境中基因转录改变的预测标记; 或可用于实现哺乳动物细胞中重组蛋白的过表达。 因此,本发明还提供用于调节基因转录的重组表达载体和宿主细胞。