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    • 6. 发明申请
    • TARGET ACTIVATED MICROTRANSFER
    • 靶向活性微转运体
    • WO2004068104A2
    • 2004-08-12
    • PCT/US0323317
    • 2003-07-23
    • US GOV HEALTH & HUMAN SERVEMMERT-BUCK MICHAEL RTANGREA MICHAEL ABONNER ROBERT FCHUAQUI RODRIGO F
    • EMMERT-BUCK MICHAEL RTANGREA MICHAEL ABONNER ROBERT FCHUAQUI RODRIGO F
    • G01N33/543G01N33/569G01N
    • G01N33/56966G01N33/543Y10T428/2813Y10T428/2839
    • A method of removing a target from a biological sample which involved placing a transfer surface in contact with the biological sample, and then focally altering the transfer surface to allow selective separation of the target from the biological sample. In disclosed embodiments, the target is a cell or cellular component of a tissue section and the transfer surface is a film that can be focally altered to adhere the target to the transfer surface. Subsequent separation of the film from the tissue section selectively removes the adhered target from the tissue section. The transfer surface is activated from within the target to adhere the target to the transfer surface, for example by heating the target to adhere or to a thermoplastic transfer surface. Such in situ activation can be achieved by exposing the biological sample to an immunoreagent that specifically binds to the target (or a component of the target). The immunoreagent can alter the transfer surface directly ( for example with a heat generating enzyme carried by the immunoreagent), or indirectly ( for example by changing a characteristic of the target). In some embodiments, the immunoreagent deposits a precipitate in the target that increases its light absorption relative to surrounding tissue, such that the biological specimen can be exposed to light to selectively heat the target. Alternatively, the immunoreagent is an immunofluorescent agent that carries a fluorosphore that absorbs light and emits heat.
    • 一种从生物样品中去除目标物的方法,其涉及将转印表面与生物样品接触,然后局部地改变转印表面以允许目标物与生物样品的选择性分离。 在公开的实施例中,目标是组织切片的细胞或细胞组分,并且转印表面是可以局部改变以将目标粘附到转印表面的膜。 随后从组织部分分离膜选择性地从组织部分移除粘附的目标。 例如通过加热靶以粘附或热塑性转印表面,转印表面从靶内激活以将靶附着到转印表面。 这种原位激活可通过将生物样品暴露于特异性结合靶标(或靶标组分)的免疫试剂来实现。 免疫试剂可以直接改变转移表面(例如用免疫试剂携带的生热酶),或间接改变(例如通过改变靶标的特征)。 在一些实施方案中,免疫试剂将沉淀物沉积在靶中,相对于周围组织增加其光吸收,使得生物样本可暴露于光以选择性地加热靶。 或者,免疫试剂是携带吸收光并发热的荧光磷光体的免疫荧光剂。