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    • 3. 发明申请
    • KDO ALDOLASE AND CONDENSATION REACTIONS EMPLOYED THEREWITH
    • KDO ALDOLASE和冷凝反应
    • WO9414954A3
    • 1994-09-29
    • PCT/US9312367
    • 1993-12-17
    • SCRIPPS RESEARCH INSTWONG CHI HUEYSUGAI TAKESHISHEN GWO JENN
    • WONG CHI-HUEYSUGAI TAKESHISHEN GWO-JENN
    • C07H7/027C12N9/88C12P7/58C12P9/00C12P19/02C07H7/02C12N1/20
    • C12P19/02C07H7/027C12N9/88C12P7/58C12P9/00Y10S435/822
    • Aureobacterium barkerei strain KDO-37-2 (ATCC 49977) KDO aldolase (EC 4.1.2.23) isolated therefrom are disclosed. The DKDO aldolase is further disclosed to have a broad substrate specificity with respect to its reverse reaction, i.e. the condensation of aldoses with pyruvate to form a wide range of 2-keto-3-deoxy-onic acids, including 2-keto-3-deoxy-nonulosonic acid, 2-keto-3-deoxy-octulosonic acid, 2-keto-3-deoxy-heptulosonic acid, and 2-keto-3-deoxy-hexulosonic acid. In particular, 3-deoxy-D-manno-2-octulosonic acid (D-KDO), a vital component of lipopolysaccharides found in the bacterial outer membrane may be synthesized from D-arabinose and pyruvate in 67 % yield. Additionally, protected forms of the KDO aldolase products, e.g. hexaacetyl 2-keto-3-deoxy-nonulosonic acid and pentaacetyl 2-keto-3-deoxy-octulosonic acid, may be decarboxylated to form the corresponding 2-deoxy-aldoses, e.g. 2-deoxy-octulose and 2-deoxy-heptulose respectively.
    • 公开了从其分离的巴氏杆菌菌株KDO-37-2(ATCC 49977)和KDO醛缩酶(EC 4.1.2.23)。 进一步公开KDO醛缩酶相对于其逆反应具有广泛的底物特异性,即醛糖与丙酮酸的缩合形成宽范围的2-酮-3-脱氧酸,包括2-酮-3- 脱氧 - 非酮酸,2-酮-3-脱氧 - 八环酮酸,2-酮-3-脱氧 - 庚酮酸和2-酮-3-脱氧 - 己酸。 特别地,在D-细菌外膜中发现的脂多糖的重要组分3-脱氧-D-壬烯-2-辛酮酸(D-KDO)可由D-阿拉伯糖和丙酮酸合成,产率67%。 另外,保护形式的KDO醛缩酶产物,例如, 六乙酰基2-酮-3-脱氧 - 非酮缩酮酸和五乙酰基2-酮-3-脱氧 - 八环酮酸可以脱羧以形成相应的2-脱氧醛糖,例如。 2-脱氧 - 八酮糖和2-脱氧 - 七氟醚。
    • 4. 发明申请
    • KDO ALDOLASE AND CONDENSATION REACTIONS EMPLOYED THEREWITH
    • KDO ALDOLASE和冷凝反应
    • WO1994014954A2
    • 1994-07-07
    • PCT/US1993012367
    • 1993-12-17
    • THE SCRIPPS RESEARCH INSTITUTEWONG, Chi-HueySUGAI, TakeshiSHEN, Gwo-Jenn
    • THE SCRIPPS RESEARCH INSTITUTE
    • C12N09/88
    • C12P19/02C07H7/027C12N9/88C12P7/58C12P9/00Y10S435/822
    • Aureobacterium barkerei strain KDO-37-2 (ATCC 49977) KDO aldolase (EC 4.1.2.23) isolated therefrom are disclosed. The DKDO aldolase is further disclosed to have a broad substrate specificity with respect to its reverse reaction, i.e. the condensation of aldoses with pyruvate to form a wide range of 2-keto-3-deoxy-onic acids, including 2-keto-3-deoxy-nonulosonic acid, 2-keto-3-deoxy-octulosonic acid, 2-keto-3-deoxy-heptulosonic acid, and 2-keto-3-deoxy-hexulosonic acid. In particular, 3-deoxy-D-manno-2-octulosonic acid (D-KDO), a vital component of lipopolysaccharides found in the bacterial outer membrane may be synthesized from D-arabinose and pyruvate in 67 % yield. Additionally, protected forms of the KDO aldolase products, e.g. hexaacetyl 2-keto-3-deoxy-nonulosonic acid and pentaacetyl 2-keto-3-deoxy-octulosonic acid, may be decarboxylated to form the corresponding 2-deoxy-aldoses, e.g. 2-deoxy-octulose and 2-deoxy-heptulose respectively.
    • 公开了从其分离的巴氏杆菌菌株KDO-37-2(ATCC 49977)KDO醛缩酶(EC 4.1.2.23)。 进一步公开了DKDO醛缩酶对于其逆反应具有广泛的底物特异性,即醛糖与丙酮酸的缩合形成宽范围的2-酮-3-脱氧酸,包括2-酮-3- 脱氧 - 非酮酸,2-酮-3-脱氧 - 八环酮酸,2-酮-3-脱氧 - 庚酮酸和2-酮-3-脱氧 - 己酸。 特别地,在D-细菌外膜中发现的脂多糖的重要组分3-脱氧-D-壬烯-2-辛酮酸(D-KDO)可由D-阿拉伯糖和丙酮酸合成,产率67%。 另外,保护形式的KDO醛缩酶产物,例如, 六乙酰基2-酮-3-脱氧 - 非酮酸和五乙酰基2-酮-3-脱氧 - 八环酸可以脱羧以形成相应的2-脱氧醛糖,例如, 2-脱氧 - 八酮糖和2-脱氧 - 七氟醚。