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1. 发明申请

WO2012023847A3 NON-LEACHABLE MAGNETIC CROSS-LINKED ENZYME AGGREGATE 审中-公开
标题翻译：不可浸出的磁性交联酶
公开(公告)号：WO2012023847A3
公开(公告)日：2013-01-17
申请号：PCT/NL2011000054
申请日：2011-07-12
申请人： CLEA TECHNOLOGIES B V , SHELDON ROGER ARTHUR , SORGEDRAGER MENNO JORT , KONDOR BERNADETT
发明人： SHELDON ROGER ARTHUR , SORGEDRAGER MENNO JORT , KONDOR BERNADETT
IPC分类号： C12N11/14 , C12N9/96
CPC分类号： C12N9/96 , C12N11/14
摘要： A non-leachable, crosslinked, on a nanometer scale formed magnetic enzyme aggregate, consisting of a non-layered, hybrid nano-composite of functionalised magnetic nanoparticles and aggregated enzyme particles, is described. The magnetic enzyme aggregate can have a high enzyme content, of up to 99%. The high enzyme content allows the use on a small scale, such as for example in a fluidised bed, of the magnetic enzyme aggregate. Also, a process for the preparation of the present magnetic enzyme aggregate is described.
摘要翻译：描述了由纳米级形成的非酶可交联的形成的磁性酶聚集体，由官能化磁性纳米颗粒和聚集的酶颗粒的非层状混合纳米复合物组成。磁性酶聚集体可以具有高达99％的高酶含量。高酶含量允许在磁性酶聚集体的小规模（例如流化床）中使用。另外，还描述了制备本发明的磁性酶聚集体的方法。

2. 发明申请

WO2012023847A2 NON-LEACHABLE MAGNETIC CROSS-LINKED ENZYME AGGREGATE 审中-公开
标题翻译：不可浸出的磁性交联酶
公开(公告)号：WO2012023847A2
公开(公告)日：2012-02-23
申请号：PCT/NL2011/000054
申请日：2011-07-12
申请人： CLEA TECHNOLOGIES B.V. , SHELDON, Roger Arthur , SORGEDRAGER, Menno Jort , KONDOR, Bernadett
发明人： SHELDON, Roger Arthur , SORGEDRAGER, Menno Jort , KONDOR, Bernadett
IPC分类号： C12N11/14 , C12N9/96
CPC分类号： C12N9/96 , C12N11/14
摘要： A non-leachable, crosslinked, on a nanometer scale formed magnetic enzyme aggregate, consisting of a non-layered, hybrid nano-composite of functionalised magnetic nanoparticles and aggregated enzyme particles, is described. The magnetic enzyme aggregate can have a high enzyme content, of up to 99%. The high enzyme content allows the use on a small scale, such as for example in a fluidised bed, of the magnetic enzyme aggregate. Also, a process for the preparation of the present magnetic enzyme aggregate is described.
摘要翻译：描述了由纳米级形成的非酶可交联的形成的磁性酶聚集体，由官能化磁性纳米颗粒和聚集的酶颗粒的非层状混合纳米复合物组成。磁性酶聚集体可以具有高达99％的高酶含量。高酶含量允许在磁性酶聚集体的小规模（例如流化床）中使用。另外，还描述了制备本发明的磁性酶聚集体的方法。

3. 发明申请

WO2004015132A3 ENZYMATIC PROCESSES FOR THE PRODUCTION OF 4-SUBSTITUTED 3-HYDROXYBUTYRIC ACID DERIVATIVES 审中-公开
标题翻译：用于生产4-取代的3-羟基丁酸衍生物的酶法
公开(公告)号：WO2004015132A3
公开(公告)日：2005-04-14
申请号：PCT/US0325263
申请日：2003-08-11
申请人： CODEXIS INC , DAVIS S CHRISTOPHER , GRATE JOHN H , GRAY DAVID R , GRUBER JOHN M , HUISMAN GJALT W , MA STEVEN K , NEWMAN LISA M , SHELDON ROGER , WANG LI A
发明人： DAVIS S CHRISTOPHER , GRATE JOHN H , GRAY DAVID R , GRUBER JOHN M , HUISMAN GJALT W , MA STEVEN K , NEWMAN LISA M , SHELDON ROGER , WANG LI A
IPC分类号： C12N9/14 , C12P13/00 , C12P13/02
CPC分类号： C12P13/002 , C12N9/14 , C12P13/02
摘要： The present invention provides methods and compositions for preparing 4-substituted 3-hydroxybutyric acid derivatives by halohydrin dehalogenase-catalyzed conversion of 4-halo-3-hydroxybutyric acid derivatives. The present invention further provides methods and compositions for preparing 4-halo-3-hydroxybutyric acid derivatives by ketoreductase-catalyzed conversion of 4-halo-3-ketobutyric acid derivatives.
摘要翻译：本发明提供了通过卤代醇脱卤酶催化的4-卤代-3-羟基丁酸衍生物的转化制备4-取代的3-羟基丁酸衍生物的方法和组合物。本发明还提供了通过酮还原酶催化转化4-卤代-3-酮丁酸衍生物制备4-卤代-3-羟基丁酸衍生物的方法和组合物。

4. 发明申请

WO2005018579A2 ENZYMATIC PROCESSES FOR THE PRODUCTION OF 4-SUBSTITUTED 3-HYDROXYBUTYRIC ACID DERIVATIVES AND VICINAL CYANO, HYDROXY SUBSTITUTED CARBOXYLIC ACID ESTERS 审中-公开
标题翻译：用于生产4-取代的3-羟基丁酸衍生物和邻氰基的羟基取代的羧酸酯的酶法
公开(公告)号：WO2005018579A2
公开(公告)日：2005-03-03
申请号：PCT/US2004004819
申请日：2004-02-18
申请人： CODEXIS INC , DAVIS S CHRISTOPHER , GRATE JOHN H , GRAY DAVID R , GRUBER JOHN M , HUISMAN GJALT W , MA STEVEN K , NEWMAN LISA M , SHELDON ROGER , WANG LI A
发明人： DAVIS S CHRISTOPHER , GRATE JOHN H , GRAY DAVID R , GRUBER JOHN M , HUISMAN GJALT W , MA STEVEN K , NEWMAN LISA M , SHELDON ROGER , WANG LI A
IPC分类号： A61K20060101 , C12N1/14 , C12N9/14 , C12P7/64 , C12P13/00 , A61K
CPC分类号： C12P13/002 , C12N9/14 , C12P7/62 , C12P13/02
摘要： The present invention provides methods and composition for preparing 4-substituted 3-hydroxybutyric acid derivatives by halohydrin dehalogenase-catalyzed conversion of 4-halo-3-hydroxybutyric acid derivatives. The present invention further provides methods and compositions for preparing 4-halo-3-hydroxybutyric acid derivatives by ketoreductase-catalyzed conversion of 4-halo-3ketobutyric acid derivatives. The present invention also provides methods and compositions for preparing vicinal cyano, hydroxyl substituted carboxylic acid esters.
摘要翻译：本发明提供了通过卤代醇脱卤素酶催化的4-卤代-3-羟基丁酸衍生物的转化来制备4-取代的3-羟基丁酸衍生物的方法和组合物。本发明进一步提供了通过酮还原酶催化的4-卤代-3-酮丁酸衍生物的转化制备4-卤代-3-羟基丁酸衍生物的方法和组合物。本发明还提供了用于制备邻位氰基，羟基取代的羧酸酯的方法和组合物。

5. 发明申请

WO2004015132A2 ENZYMATIC PROCESSES FOR THE PRODUCTION OF 4-SUBSTITUTED 3-HYDROXYBUTYRIC ACID DERIVATIVES 审中-公开
标题翻译：用于生产4-取代的3-羟基丁酸衍生物的酶法
公开(公告)号：WO2004015132A2
公开(公告)日：2004-02-19
申请号：PCT/US2003/025263
申请日：2003-08-11
申请人： CODEXIS, INC. , DAVIS, S., Christopher , GRATE, John, H. , GRAY, David, R. , GRUBER, John, M. , HUISMAN, Gjalt, W. , MA, Steven, K. , NEWMAN, Lisa, M. , SHELDON, Roger , WANG, Li, A.
发明人： DAVIS, S., Christopher , GRATE, John, H. , GRAY, David, R. , GRUBER, John, M. , HUISMAN, Gjalt, W. , MA, Steven, K. , NEWMAN, Lisa, M. , SHELDON, Roger , WANG, Li, A.
IPC分类号： C12Q
CPC分类号： C12P13/002 , C12N9/14 , C12P13/02
摘要： The present invention provides methods and compositions for preparing 4-substituted 3-hydroxybutyric acid derivatives by halohydrin dehalogenase-catalyzed conversion of 4-halo-3-hydroxybutyric acid derivatives. The present invention further provides methods and compositions for preparing 4-halo-3-hydroxybutyric acid derivatives by ketoreductase-catalyzed conversion of 4-halo-3-ketobutyric acid derivatives.
摘要翻译：本发明提供了通过卤代醇脱卤酶催化的4-卤代-3-羟基丁酸衍生物的转化来制备4-取代的3-羟基丁酸衍生物的方法和组合物。本发明还提供了通过酮还原酶催化转化4-卤代-3-酮丁酸衍生物制备4-卤代-3-羟基丁酸衍生物的方法和组合物。

6. 发明申请

WO0220821A3 PROCESS FOR THE PREPARATION OF ENANTIOMERICALLY ENRICHED AMINES 审中-公开
标题翻译：制备高效液相色谱的方法
公开(公告)号：WO0220821A3
公开(公告)日：2002-08-08
申请号：PCT/NL0100643
申请日：2001-08-31
申请人： DSM NV , SVEDAS VYTAUTAS-JUOZAPAS KAJET , GURANDA DOREL TEODOR , KHIMIOUK ANDREI IAROSLAVONICH , SHELDON ROGER ARTHUR , VAN RANTWIJK FREDERIK , VAN LANGEN LUKAS MICHAEL
发明人： SVEDAS VYTAUTAS-JUOZAPAS KAJET , GURANDA DOREL TEODOR , KHIMIOUK ANDREI IAROSLAVONICH , SHELDON ROGER ARTHUR , VAN RANTWIJK FREDERIK , VAN LANGEN LUKAS MICHAEL
IPC分类号： C12P13/00 , C12P13/02 , C12P13/04 , C12P41/00 , C07C237/06
CPC分类号： C12P13/002 , C12P13/008 , C12P13/02 , C12P13/04 , C12P41/006 , C12P41/007
摘要： Process for the preparation of an enantiomerically enriched acylated amine, wherein an enantiomerically enriched, substituted or unsubstituted phenylglycine amide is contacted with a mixture of enantiomers of the corresponding amine H2NCR1R2R3 where R1, R2 and R3 are not the same and each independently stands for H, CN, a substituted (cyclo)alkyl group, aryl group, alkylaryl group or arylalkyl group, a cyclic or non-cyclic heteroaryl group or heteroaryl group with one or more N, O of S atoms or (CH2)n-COR4, where n=0, 1 ...6 and R4 is OH or a substituted or unsubstituted alkyl group, aryl group, alkoxy group or amino group or where R1 and R2 (and R3) together with the C atom to which they are bound form a (bi)cyclic group which may or may not contain one or more N, O of S atoms, in the presence of a Pen-G acylase. A process for the preparation of enantiomerically enriched amine with formula H2NCR1R2R3 where R1, R2 and R3 are as defined above, wherein an acylated amine is contacted with a Pen-G acylase. Preferably a Pen-G acylase derived from Alcaligenes faecalis is applied.
摘要翻译：制备对映异构体富集的酰化胺的方法，其中将对映异构体富集的取代或未取代的苯基甘氨酸酰胺与相应的胺H 2 NCR 1 R 2 R 3的对映异构体的混合物接触，其中R1，R2和R3不相同，并且各自独立地代表H， CN，取代的（环）烷基，芳基，烷基芳基或芳烷基，具有一个或多个S原子或（CH 2）n-COR 4的N，O的环状或非环状杂芳基或杂芳基，其中n = 0,1 ... 6，R4是OH或取代或未取代的烷基，芳基，烷氧基或氨基，或者其中R1和R2（和R3）与它们所结合的C原子一起形成（ bi）环基团，其可以含有或不含有一个或多个S原子的N，O，在Pen-G酰基转移酶存在下。制备具有式H2NCR1R2R3的对映异构体富集的胺的方法，其中R1，R2和R3如上定义，其中酰化的胺与Pen-G酰基转移酶接触。优选地，应用来自粪肠杆菌的Pen-G酰基转移酶。

7. 发明申请

WO1995007359A1 A PROCESS FOR CARRYING OUT ENZYMATICALLY CATALYZED CONVERSIONS OF ORGANIC COMPOUNDS 审中-公开
标题翻译：进行有机化合物的酶催化转化的方法
公开(公告)号：WO1995007359A1
公开(公告)日：1995-03-16
申请号：PCT/EP1994003038
申请日：1994-09-12
申请人： TECHNISCHE UNIVERSITEIT DELFT , DE ZOETE, Maria, Christina , VAN RANTWIJK, Frederik , SHELDON, Roger, Arthur
发明人： TECHNISCHE UNIVERSITEIT DELFT
IPC分类号： C12P13/02
CPC分类号： C12P41/006 , C12P13/02
摘要： A process for carrying out enzymatically catalyzed conversions of organic compounds comprises reacting an ester of an organic acid in a liquid organic phase with ammonia in the presence of a hydrolytic enzyme such as a lipase, esterase or protease. The process permits the highly enantioselective preparation of enantiomers of amides, esters and/or alcohols in good yield from racemic starting material under very mild reaction conditions.
摘要翻译：用于进行有机化合物的酶催化转化的方法包括在水解酶如脂肪酶，酯酶或蛋白酶的存在下，将液体有机相中的有机酸的酯与氨反应。该方法允许在非常温和的反应条件下从外消旋原料高度对映选择性地制备酰胺，酯和/或醇的对映异构体。

8. 发明申请

WO2005001107A1 METHOD OF SEPARATING OPTICAL ISOMERS OF A PROTECTED AMINOACID 审中-公开
标题翻译：分离保护氨基酸的光学异构体的方法
公开(公告)号：WO2005001107A1
公开(公告)日：2005-01-06
申请号：PCT/RU2004/000244
申请日：2004-06-25
申请人： CLEA TECHNOLOGIES BV , BIOTIR LTD , YOUSHKO, Maxim Ilich , SVEDAS, Vytautas-Juozapas Kajetono , SHELDON, Roger, Arthur , VAN LANGEN, Lukas, Michael
发明人： YOUSHKO, Maxim Ilich , SVEDAS, Vytautas-Juozapas Kajetono , SHELDON, Roger, Arthur , VAN LANGEN, Lukas, Michael
IPC分类号： C12P41/00
CPC分类号： C07K14/38 , C12P13/04 , C12P41/005 , C12P41/007 , Y02P20/55
摘要： The invention relates to a method Method of enzymatically separating optical isomers of a protected amino acid. According to the invention, a (non-sidechain-carboxyl) protected aminoacid is used having the general formula (I) HR 1 N-CR 2 - (CH) n -C (0) ZR 3 wherein R 1 is an amino-protective group or hydrogen; - R 2 is a side chain; n is a number chosen from 0, 1, and 2; ZR 3 is a carboxy-protective group where Z is chosen from 0 or NH; and R 1 and R 3 may be integrated, forming a heterocyclic (non-sidechain-carboxyl)-protected ring, the ring backbone comprising 5 to 8 atoms; the (non-sidechain-carboxyl)-protected aminoacid having a molecular weight of less than 1000 Dalton, said method comprising the steps of - contacting the (non-sidechain-carboxyl) -protected aminoacid (I) in the presence of water with an optionally partially purified cell-free extract of a fungus containing an enzyme having at least one of an esterase and amidase activity yielding a mixture of (non-sidechain carboxyl) deprotected product and starting material; and - optionally separating the (non-sidechain carboxyl) deprotected product and starting material.
摘要翻译：本发明涉及一种酶法分离被保护氨基酸的光学异构体的方法。根据本发明，使用具有通式（I）HR 1 N-CR 2 - （CH）n C（O）Z R 3的（非侧链羧基）保护的氨基酸，其中R 1 >是氨基保护基或氢; -R 2是侧链; n是选自0,1和2的数字; ZR 3是羧基保护基，其中Z选自0或NH; 并且R 1和R 3可以整合，形成杂环（非侧链羧基）保护的环，所述环骨架包含5-8个原子; 分子量小于1000道尔顿的（非侧链羧基）保护的氨基酸，所述方法包括以下步骤：在水存在下使（非侧链羧基）保护的氨基酸（I）与任选地部分纯化的无细胞提取物，其含有具有酯酶和酰胺酶活性中至少一种的酶，产生（非侧链羧基）脱保护产物和起始材料的混合物; 和 - 任选分离（非侧链羧基）脱保护产物和起始原料。

9. 发明申请

WO02102742A3 METHOD FOR THE CATALYTIC OXIDATIVE SEPARATION OF CYCLIC SATURATED AND UNSATURATED COMPOUNDS 审中-公开
标题翻译： PROCESS FOR CATALYTIC氧化裂解CYCLICAL饱和和不饱和化合物
公开(公告)号：WO02102742A3
公开(公告)日：2003-09-18
申请号：PCT/EP0206299
申请日：2002-06-10
申请人： CREAVIS TECH & INNOVATION GMBH , KUEHNLE ADOLF , JOST CARSTEN , SHELDON ROGER ARTHUR , CHATEL SANDRINE M M , ARENDS ISABELLA W C E
发明人： KUEHNLE ADOLF , JOST CARSTEN , SHELDON ROGER ARTHUR , CHATEL SANDRINE M M , ARENDS ISABELLA W C E
IPC分类号： C07B41/08 , C07C51/31 , C07D233/72 , C07C55/02 , C07C55/14 , C07C55/21
CPC分类号： C07C51/313 , C07B41/08 , C07C55/21 , C07C55/14
摘要： The invention relates to a method for the catalytic oxidative separation, in the presence of a radical initiator, of cyclic hydrocarbons and heterocyclic compounds using an acidic compound as the co-catalyst and simultaneously using a catalyst. The catalyst used is a compound of formula (I), wherein R , R represent H, an aliphatic or aromatic alkoxy group, a carboxyl group, an alkoxycarbonyl group or a hydrocarbon group having 1 to 20 carbon atoms each, SO3H, NH2, OH, F, Cl, Br, I and/or NO2, wherein R and R may represent identical or different groups or R and R are linked via a covalent bond, wherein Q , Q are the same or different, C, CH, N; X, Z represent C, S or CH2; Y represents O or OH; k is 0, 1 or 2; l is 0, 1 or 2, whereby k and l may not be 0 at the same time; m is 1 to 100. The molar ratio of the catalyst to the hydrocarbon is less than 10 mole percent. The radical initiator used can be peroxy compounds or azo compounds. Preferred substrates are cyclic aliphatic or aromatic compounds.
摘要翻译：本发明涉及一种用于循环基材如环烃和杂环化合物，使用酸性化合物作为助催化剂和同时的催化氧化裂解的方法，使用催化剂，其中，作为催化剂的式（I）中R <1>，R的化合物 <2> = H，脂族或芳族烷氧基基团，羧基基团，烷氧基羰基或烃基，各自具有1至20个碳原子，SO 3 H，NH 2，OH，氟，氯，Br，I和/或NO 2，其中R <1>和R < 2表示>相同或不同的基团或R可以经由共价键连接在一起<1>和R <2>，Q <1>，Q <2>相同或不同，C，CH，N; X，Z = C，S或CH 2; Y = O或OH; K = 0，1或2; L = 0，1或2，其中k和l不同时为值0; M = 1至100，是在自由基引发剂，其特征在于，所述催化剂与烃的摩尔比小于10摩尔％的存在下使用。自由基引发剂过氧或者可以使用偶氮化合物。优选的基材是环状脂肪族或芳族化合物。

10. 发明申请

WO2002061067A1 A METHOD OF PREPARING CROSS-LINKED ENZYME AGGREGATES 审中-公开
标题翻译：一种制备交联酶的方法
公开(公告)号：WO2002061067A1
公开(公告)日：2002-08-08
申请号：PCT/NL2002/000079
申请日：2002-02-01
申请人： TECHNISCHE UNIVERSITEIT DELFT , LÓPEZ SERRANO, Paloma , CAO, Linqiu , VAN RANTWIJK, Frederik , SHELDON, Roger, Arthur
发明人： LÓPEZ SERRANO, Paloma , CAO, Linqiu , VAN RANTWIJK, Frederik , SHELDON, Roger, Arthur
IPC分类号： C12N9/96
CPC分类号： C12P7/00 , C12N9/20 , C12N9/96 , C12N11/02 , C12Y301/01003
摘要： The invention relates to a method of preparing cross-linked enzyme aggregates (CLEAs) comprising the steps of a) precipitating a dissolved enzyme present in a liquid medium; b) cross-linking with a cross-linker, and c) optionally, washing the cross-linked enzyme aggregate. According to the invention at least one treatment is performed chosen from the group consisting of I) performing step a) in the presence of a compound selected from the group consisting of i) a crown ether, and ii) a surfactant, and II) performing step c) with a second liquid differing in organic composition from the liquid.
摘要翻译：本发明涉及一种制备交联酶聚集体（CLEA）的方法，包括以下步骤：a）沉淀存在于液体介质中的溶解酶; b）与交联剂交联，和c）任选地，洗涤交联的酶聚集体。根据本发明，进行至少一种处理，选自I）在选自i）冠醚和ii）表面活性剂的化合物的存在下进行步骤a），和II）进行步骤c）与来自液体的有机组成不同的第二液体。

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