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1. 发明申请

WO1998019528A1 CELL WASH APPARATUS AND METHOD 审中-公开
标题翻译：电池洗衣机和方法
公开(公告)号：WO1998019528A1
公开(公告)日：1998-05-14
申请号：PCT/US1997012130
申请日：1997-06-19
申请人： CHASE, Eric , NORTH, Howard, L., Jr. , SCHULTE, Harvey
IPC分类号： A01N01/02
CPC分类号： G01N1/40 , B04B5/0407 , B04B5/0442 , Y10T436/25 , Y10T436/25375 , Y10T436/255 , Y10T436/2575
摘要： An apparatus and method are disclosed to wash blood cells in a discrete manner that is compatible with automated sample preparation systems. The test tube containing the cells to be washed is mounted on a rotatable spindle. The spindle includes central passageways for the introduction of wash fluid and air into the test tube, and radial exit passageways at the bottom of the spindle. The test tube is first spun about its vertical axis to centrifuge cells against the inner wall of the test tube. A vacuum is then applied to the exit passageways so cell supernatant is aspirated out through the exit passageways. Wash fluid is then introduced into the test tube, and aspirated out through the exit passageways, thereby washing the cells. Rotational acceleration and deceleration of the test tube then resuspends the cells in wash fluid.
摘要翻译：公开了以与自动化样品制备系统相容的离散方式洗涤血细胞的装置和方法。将含有要洗涤的电池的试管安装在可旋转的主轴上。主轴包括用于将洗涤流体和空气引入试管的中心通道，以及主轴底部的径向出口通道。试管首先围绕其垂直轴旋转，将细胞离心到试管的内壁。然后将真空施加到出口通道，使得细胞上清液通过出口通道被吸出。然后将洗涤液引入试管中，并通过出口通道吸出，从而洗涤细胞。然后试管的旋转加速和减速将细胞重新悬浮在洗涤液中。

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