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    • 2. 发明申请
    • ACETYL-COA PRODUCING ENZYMES IN YEAST
    • 乙酰乙酸生产酶在酵母中的应用
    • WO2009013159A2
    • 2009-01-29
    • PCT/EP2008059119
    • 2008-07-11
    • DSM IP ASSETS BVMUELLER ULRIKE MARIAWU LIANGRAAMSDONK LOURINA MADELEINEWINKLER AARON ADRIAAN
    • MUELLER ULRIKE MARIAWU LIANGRAAMSDONK LOURINA MADELEINEWINKLER AARON ADRIAAN
    • C12N15/81C12P7/16
    • C12Q1/32C07K14/33C12N9/001C12P7/16Y02E50/10
    • The present invention relates to a method of identifying a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) a yeast cell comprising: a) providing a mutated yeast cell comprising a deletion of at least one gene of the (PDH) by-pass, selected from the genes encoding the enzymes pyruvate decarboxylase (PDC), acetaldehyde dehydrogenase (ALD), and acetyl-CoA synthetase (ACS); b) transforming said mutated yeast cell with an expression vector comprising a heterologous nucleotide sequence encoding a candidate polypeptide having potential enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA; c) testing said recombinant mutated yeast cell for its ability to grow on minimal medium containing glucose as sole carbon source, and d) identifying said candidate polypeptide as a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) said yeast cell when growth of said cell is observed. The invention further relates to a method of producing a fermentation production such as butanol.
    • 本发明涉及鉴定具有酶促活性的异源多肽的方法,所述酶促活性用于将丙酮酸,乙醛或乙酸转化为酵母细胞(的胞质溶胶)中的乙酰-CoA,所述方法包括:a)提供突变的酵母细胞,所述突变的酵母细胞包含至少 选自编码丙酮酸脱羧酶(PDC),乙醛脱氢酶(ALD)和乙酰-CoA合成酶(ACS)的基因的(PDH)旁路的一个基因; b)用表达载体转化所述突变的酵母细胞,所述表达载体包含编码具有用于将丙酮酸,乙醛或乙酸转化成乙酰-CoA的潜在酶活性的候选多肽的异源核苷酸序列; c)测试所述重组突变的酵母细胞在含有葡萄糖作为唯一碳源的基本培养基上生长的能力,和d)将所述候选多肽鉴定为具有用于将丙酮酸,乙醛或乙酸转化为乙酰辅酶A的酶活性的异源多肽 当观察到所述细胞的生长时,所述酵母细胞的胞质溶胶)。 本发明进一步涉及生产发酵生产如丁醇的方法。