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1. 发明申请

WO2009013157A1 BUTANOL PRODUCTION IN A EUKARYOTIC CELL 审中-公开
标题翻译：丁醇生产在一个欧洲细胞
公开(公告)号：WO2009013157A1
公开(公告)日：2009-01-29
申请号：PCT/EP2008/059117
申请日：2008-07-11
申请人： DSM IP ASSETS B.V. , MUELLER, Ulrike, Maria , WU, Liang , RAAMSDONK, Lourina, Madeleine
发明人： MUELLER, Ulrike, Maria , WU, Liang , RAAMSDONK, Lourina, Madeleine
IPC分类号： C12P7/16 , C12N15/81 , C12N9/02
CPC分类号： C12Q1/32 , C07K14/33 , C12N9/001 , C12P7/16 , Y02E50/10
摘要： The present invention relates to a yeast cell capable of producing butanol, comprising a nucleotide sequence encoding a butyryl-CoA dehydrogenase and at least one nucleotide sequence encoding an electron transfer flavoprotein. The invention further relates to a process for the production of butanol and the use of butanol obtainable by the process according to the present invention as a chemical or as a fuel.
摘要翻译：本发明涉及能够生产丁醇的酵母细胞，其包含编码丁酰辅酶A脱氢酶的核苷酸序列和编码电子转移黄素蛋白的至少一个核苷酸序列。本发明还涉及一种生产丁醇的方法和通过本发明的方法获得的丁醇作为化学或燃料的用途。

2. 发明申请

WO2009013159A2 ACETYL-COA PRODUCING ENZYMES IN YEAST 审中-公开
标题翻译：乙酰乙酸生产酶在酵母中的应用
公开(公告)号：WO2009013159A2
公开(公告)日：2009-01-29
申请号：PCT/EP2008059119
申请日：2008-07-11
申请人： DSM IP ASSETS BV , MUELLER ULRIKE MARIA , WU LIANG , RAAMSDONK LOURINA MADELEINE , WINKLER AARON ADRIAAN
发明人： MUELLER ULRIKE MARIA , WU LIANG , RAAMSDONK LOURINA MADELEINE , WINKLER AARON ADRIAAN
IPC分类号： C12N15/81 , C12P7/16
CPC分类号： C12Q1/32 , C07K14/33 , C12N9/001 , C12P7/16 , Y02E50/10
摘要： The present invention relates to a method of identifying a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) a yeast cell comprising: a) providing a mutated yeast cell comprising a deletion of at least one gene of the (PDH) by-pass, selected from the genes encoding the enzymes pyruvate decarboxylase (PDC), acetaldehyde dehydrogenase (ALD), and acetyl-CoA synthetase (ACS); b) transforming said mutated yeast cell with an expression vector comprising a heterologous nucleotide sequence encoding a candidate polypeptide having potential enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA; c) testing said recombinant mutated yeast cell for its ability to grow on minimal medium containing glucose as sole carbon source, and d) identifying said candidate polypeptide as a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) said yeast cell when growth of said cell is observed. The invention further relates to a method of producing a fermentation production such as butanol.
摘要翻译：本发明涉及鉴定具有酶促活性的异源多肽的方法，所述酶促活性用于将丙酮酸，乙醛或乙酸转化为酵母细胞（的胞质溶胶）中的乙酰-CoA，所述方法包括：a）提供突变的酵母细胞，所述突变的酵母细胞包含至少选自编码丙酮酸脱羧酶（PDC），乙醛脱氢酶（ALD）和乙酰-CoA合成酶（ACS）的基因的（PDH）旁路的一个基因; b）用表达载体转化所述突变的酵母细胞，所述表达载体包含编码具有用于将丙酮酸，乙醛或乙酸转化成乙酰-CoA的潜在酶活性的候选多肽的异源核苷酸序列; c）测试所述重组突变的酵母细胞在含有葡萄糖作为唯一碳源的基本培养基上生长的能力，和d）将所述候选多肽鉴定为具有用于将丙酮酸，乙醛或乙酸转化为乙酰辅酶A的酶活性的异源多肽当观察到所述细胞的生长时，所述酵母细胞的胞质溶胶）。本发明进一步涉及生产发酵生产如丁醇的方法。

3. 发明申请

WO2008052991A2 BUTANOL PRODUCTION IN A EUKARYOTIC CELL 审中-公开
标题翻译：丁醇在真核细胞中的产生
公开(公告)号：WO2008052991A2
公开(公告)日：2008-05-08
申请号：PCT/EP2007/061685
申请日：2007-10-30
申请人： DSM IP ASSETS B.V. , RAAMSDONK, Lourina, Madeleine , LAAT, DE, Wilhelmus, Theodorus, Antonius, Maria , BERG, VAN DEN, Marco, Alexander
发明人： RAAMSDONK, Lourina, Madeleine , LAAT, DE, Wilhelmus, Theodorus, Antonius, Maria , BERG, VAN DEN, Marco, Alexander
IPC分类号： C12N15/53 , C12N15/54 , C12N15/60 , C12P7/16 , C12N1/18 , C12N9/02 , C12N9/04 , C12N9/10 , C12N9/88
CPC分类号： C12P7/16 , C12N1/18 , C12N9/0006 , C12N9/0008 , C12N9/001 , C12N9/1029 , C12N9/88 , C12Y102/0101 , C12Y103/99002 , C12Y203/01009 , Y02E50/10
摘要： The present invention relates to a transformed eukaryotic cell comprising one or more nucleotide sequence(s) encoding acetyl-CoA acetyltransferase, 3-hydroxybutyryl-CoA dehydrogenase, 3-hydroxybutyryl-CoA dehydratase, butyryl-CoA dehydrogenase, alcohol dehydrogenase or acetaldehyde dehydrogenase and/or NAD(P)H-dependent butanol dehydrogenase, whereby the nucleotide sequence(s) upon transformation of the cell confer(s) the cell the ability to produce butanol. The invention also relates to a process for the production of butanol.
摘要翻译：本发明涉及包含编码乙酰-CoA乙酰转移酶，3-羟基丁酰-CoA脱氢酶，3-羟基丁酰-CoA脱水酶，丁酰-CoA脱氢酶的一种或多种核苷酸序列的转化的真核细胞，乙醇脱氢酶或乙醛脱氢酶和/或NAD（P）H依赖性丁醇脱氢酶，由此细胞转化后的核苷酸序列赋予细胞产生丁醇的能力。本发明还涉及生产丁醇的方法。

4. 发明申请

WO2009013159A3 ACETYL-COA PRODUCING ENZYMES IN YEAST 审中-公开
公开(公告)号：WO2009013159A3
公开(公告)日：2009-01-29
申请号：PCT/EP2008/059119
申请日：2008-07-11
申请人： DSM IP ASSETS B.V. , MUELLER, Ulrike, Maria , WU, Liang , RAAMSDONK, Lourina, Madeleine , WINKLER, Aaron Adriaan
发明人： MUELLER, Ulrike, Maria , WU, Liang , RAAMSDONK, Lourina, Madeleine , WINKLER, Aaron Adriaan
IPC分类号： C12N15/81 , C12P7/16
摘要： The present invention relates to a method of identifying a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) a yeast cell comprising: a) providing a mutated yeast cell comprising a deletion of at least one gene of the (PDH) by-pass, selected from the genes encoding the enzymes pyruvate decarboxylase (PDC), acetaldehyde dehydrogenase (ALD), and acetyl-CoA synthetase (ACS); b) transforming said mutated yeast cell with an expression vector comprising a heterologous nucleotide sequence encoding a candidate polypeptide having potential enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA; c) testing said recombinant mutated yeast cell for its ability to grow on minimal medium containing glucose as sole carbon source, and d) identifying said candidate polypeptide as a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) said yeast cell when growth of said cell is observed. The invention further relates to a method of producing a fermentation production such as butanol.

5. 发明申请

WO2009013158A1 BUTANOL PRODUCTION IN A EUKARYOTIC CELL 审中-公开
标题翻译：丁醇生产在一个欧洲细胞
公开(公告)号：WO2009013158A1
公开(公告)日：2009-01-29
申请号：PCT/EP2008/059118
申请日：2008-07-11
申请人： DSM IP ASSETS B.V. , MUELLER, Ulrike, Maria , RAAMSDONK, Lourina, Madeleine
发明人： MUELLER, Ulrike, Maria , RAAMSDONK, Lourina, Madeleine
IPC分类号： C12P7/16 , C12N15/81 , C12N9/02
CPC分类号： C12P7/16 , C12N9/0006 , Y02E50/10
摘要： The present invention relates to a eukaryotic cell capable of producing butanol, wherein at least one nucleotide sequence encoding an enzyme that is capable of catalysing the conversion from acetaldehyde to ethanol is inactivated. The invention further relates to a process for the production of butanol, and the use of butanol as a chemical or as a fuel.
摘要翻译：本发明涉及能够生产丁醇的真核细胞，其中至少一个编码能够催化从乙醛转化为乙醇的酶的核苷酸序列失活。本发明还涉及一种生产丁醇的方法，以及使用丁醇作为化学物质或燃料。

6. 发明申请

WO2007006593A2 MUTANT EXPANDASES 审中-公开
标题翻译：突变事件
公开(公告)号：WO2007006593A2
公开(公告)日：2007-01-18
申请号：PCT/EP2006/061479
申请日：2006-04-10
申请人： DSM IP ASSETS B.V. , SCHIPPER, Dick , KERKMAN, Richard , RAAMSDONK, Lourina, Madeleine , BOVENBERG, Roelof, Ary, Lans
发明人： SCHIPPER, Dick , KERKMAN, Richard , RAAMSDONK, Lourina, Madeleine , BOVENBERG, Roelof, Ary, Lans
IPC分类号： C12P37/00 , C12N9/02
CPC分类号： C12P37/00 , C12N9/0069
摘要： The present invention relates to a mutant expandase that is a variant of a model polypeptide with expandase activity whereby the mutant expandase has an at least 2.5-fold improved in vitro expandase activity towards adipyl-6-APA in comparison with a model polypeptide with expandase activity.
摘要翻译：本发明涉及突变扩增酶，其是具有扩展酶活性的模型多肽的变体，其中突变扩增酶与具有扩展酶活性的模型多肽相比具有至己二酰基-6-APA的体外扩增酶活性至少2.5倍的改善。

7. 发明申请

WO2009013160A2 PROCESS FOR THE PREPARATION OF BUTANOL AND ETHANOL 审中-公开
标题翻译：丁醇和乙醇的制备方法
公开(公告)号：WO2009013160A2
公开(公告)日：2009-01-29
申请号：PCT/EP2008059120
申请日：2008-07-11
申请人： DSM IP ASSETS BV , DIJK EINTE-KARST , RAAMSDONK LOURINA MADELEINE
发明人： DIJK EINTE-KARST , RAAMSDONK LOURINA MADELEINE
IPC分类号： C12P7/16 , C12N9/02 , C12N15/81
CPC分类号： C12P7/16 , B01D3/002 , C12P7/06 , C12R1/865 , Y02E50/10 , Y02E50/17
摘要： The present invention relates to a eukaryotic cell capable of producing butanol and ethanol at a ratio butanol: ethanol of between 1:2 to 1:100. The present invention further relates to a process for the preparation of butanol and ethanol comprising fermenting a eukaryotic cell in a suitable fermentation broth, wherein butanol and ethanol are produced at a ratio butanol: hethanol of between 1:2 to 1:100 and a process for the recovery of butanol and ethanol from an aqueous solution comprising butanol and ethanol wherein the ratio butanol: ethanol is between 1:2 to 1:100 comprising, separating an ethanol/butanol/water mixture from the aqueous solution; separating an ethanol/water mixture from the ethanol/butanol/water mixture; separating a butanol/water mixture from the ethanol/butanol/water mixture; and recovering of butanol and ethanol.
摘要翻译：本发明涉及能够以1:2至1:100的丁醇/乙醇比例生产丁醇和乙醇的真核细胞。本发明还涉及一种制备丁醇和乙醇的方法，包括在合适的发酵液中发酵真核细胞，其中丁醇和乙醇以1:2至1:100的丁醇：乙醇的比例生产，用于从包含丁醇和乙醇的水溶液中回收丁醇和乙醇，其中丁醇：乙醇的比例为1:2至1:100，包括从水溶液中分离出乙醇/丁醇/水混合物; 从乙醇/丁醇/水混合物中分离乙醇/水混合物; 从乙醇/丁醇/水混合物中分离出丁醇/水混合物; 并回收丁醇和乙醇。

8. 发明申请

WO2007122249A1 PRODUCTION OF COMPOUNDS IN A RECOMBINANT HOST 审中-公开
标题翻译：在重组生物中生产化合物
公开(公告)号：WO2007122249A1
公开(公告)日：2007-11-01
申请号：PCT/EP2007/054045
申请日：2007-04-25
申请人： DSM IP Assets B.V. , BOVENBERG, Roelof, Ary, Lans , VAN DEN BERG, Marco Alexander , HAGE, Suzanne , KLAASSEN, Paul , MEIJRINK, Bernard , RAAMSDONK, Lourina, Madeleine
发明人： BOVENBERG, Roelof, Ary, Lans , VAN DEN BERG, Marco Alexander , HAGE, Suzanne , KLAASSEN, Paul , MEIJRINK, Bernard , RAAMSDONK, Lourina, Madeleine
IPC分类号： C07K14/385 , C12N1/16
CPC分类号： C12N9/1029 , C12N9/0004 , C12N9/93 , C12P7/02 , C12P13/02 , C12P17/06 , C12P17/181 , C12P17/182 , C12P17/188
摘要： The present invention provides a recombinant Penicillium chrysogenum strain characterized in that a gene selected from the list consisting of penDE, pcbAB and pcbC is inactivated and the use of such a strain for the preparation of a compound of interest. Furthermore, it is an object of the present invention to provide a method for the production of a compound of interest in a eukaryotic recombinant microorganism comprising the steps of : (a) Reducing the level of secondary metabolite production in said microorganism with 50-100%; (b) Introducing a heterologous gene into said microorganism
摘要翻译：本发明提供重组产黄青霉菌株，其特征在于，从PenDE，pcbAB和pcbC列表中选出的基因被灭活，并且使用这样的菌株来制备感兴趣的化合物。此外，本发明的目的是提供一种在真核重组微生物中生产感兴趣的化合物的方法，包括以下步骤：（a）降低所述微生物中次级代谢产物的产生水平，其中50-100％ ; （b）将异源基因引入所述微生物

9. 发明申请

WO2007006593A3 MUTANT EXPANDASES AND THEIR USE IN THE PRODUCTION OF BETA-LACTAM COMPOUNDS 审中-公开
标题翻译：突变体的发生及其在β-乳糖化合物生产中的应用
公开(公告)号：WO2007006593A3
公开(公告)日：2007-04-12
申请号：PCT/EP2006061479
申请日：2006-04-10
申请人： DSM IP ASSETS BV , SCHIPPER DICK , KERKMAN RICHARD , RAAMSDONK LOURINA MADELEINE , BOVENBERG ROELOF ARY LANS
发明人： SCHIPPER DICK , KERKMAN RICHARD , RAAMSDONK LOURINA MADELEINE , BOVENBERG ROELOF ARY LANS
IPC分类号： C12P37/00 , C12N9/02
CPC分类号： C12P37/00 , C12N9/0069
摘要： The present invention relates to a mutant expandase that is a variant of a model polypeptide with expandase activity whereby the mutant expandase has an at least 2.5-fold improved in vitro expandase activity towards adipyl-6-APA in comparison with a model polypeptide with expandase activity.
摘要翻译：本发明涉及突变扩增酶，其是具有扩展酶活性的模型多肽的变体，其中突变扩增酶与具有扩展酶活性的模型多肽相比具有至己二酰基-6-APA的体外扩增酶活性至少2.5倍的改善。

10. 发明申请

WO2006040313A3 MUTANT EXPANDASES 审中-公开
公开(公告)号：WO2006040313A3
公开(公告)日：2006-04-20
申请号：PCT/EP2005/055148
申请日：2005-10-11
申请人： DSM IP ASSETS B.V. , SCHIPPER, Dick , KERKMAN, Richard , RAAMSDONK, Lourina, Madeleine , BOVENBERG, Roelof, Ary, Lans , JENNE, Stephane J. , CHEN, Yan , CHEN, Yong Hong , GAO, Xiaodong , KREBBER, Anke , KREBBER, Claus , TO LA CHING, Charlene , TOBIN, Matthew
发明人： SCHIPPER, Dick , KERKMAN, Richard , RAAMSDONK, Lourina, Madeleine , BOVENBERG, Roelof, Ary, Lans , JENNE, Stephane J. , CHEN, Yan , CHEN, Yong Hong , GAO, Xiaodong , KREBBER, Anke , KREBBER, Claus , TO LA CHING, Charlene , TOBIN, Matthew
IPC分类号： C12N9/02 , C12P35/00 , C12N15/53
摘要： The present invention relates to a mutant expandase that is a variant of a model polypeptide with expandase activity whereby the mutant expandase has an at least 2-fold improved in vitro expandase activity towards adipyl-6-APA in comparison with the model polypeptide with expandase activity.

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