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1. 发明申请

WO2020097933A1 VOLTAGE SENSOR AND APPARATUS 审中-公开
公开(公告)号：WO2020097933A1
公开(公告)日：2020-05-22
申请号：PCT/CN2018/115999
申请日：2018-11-16
申请人： ABB SCHWEIZ AG , WU, Liang , DENG, Zhonghua
发明人： WU, Liang , DENG, Zhonghua
IPC分类号： G01R15/18 , G01R15/16 , G01R19/00 , G01R31/00
摘要： Embodiments of present disclosure relate to a voltage sensor(10) and an apparatus(11) including the voltage sensor(10). The voltage sensor(10) comprises a first capacitor(12) configured to receive a first voltage for a first phase of an alternating current supply system(1), a second capacitor(14) coupled between the first capacitor(12) and a reference voltage and configured to generate a second voltage based on the first voltage; and a transformer(16) comprising a first winding(164) coupled in parallel to the second capacitor(14) and a second winding(166) magnetically coupled to the first winding(164), the transformer(16) configured to generate a third voltage directly for a field testing unit without conversion of voltage by a further transformer. The third voltage is based on the second voltage and below the second voltage.

2. 发明申请

WO2009101180A3 PROCESS FOR THE PRODUCTION OF A DICARBOXYLIC ACID 审中-公开
标题翻译：生产二羧酸的方法
公开(公告)号：WO2009101180A3
公开(公告)日：2009-11-26
申请号：PCT/EP2009051717
申请日：2009-02-13
申请人： DSM IP ASSETS BV , VERWAAL RENE , WU LIANG , DAMVELD ROBBERTUS ANTONIUS , SAGT CORNELIS MARIA JACOBUS
发明人： VERWAAL RENE , WU LIANG , DAMVELD ROBBERTUS ANTONIUS , SAGT CORNELIS MARIA JACOBUS
IPC分类号： C12P7/46
CPC分类号： C12P7/46
摘要： The present invention relates to a process for the production of a dicarboxylic acid wherein a eukaryotic cell is fermented in a suitable fermentation medium. The invention further relates to a eukaryotic cell comprising a nucleotide sequence encoding an enzyme which catalyses the conversion of isocitric acid to succinic acid, and a nucleotide sequence encoding an enzyme which catalyses the conversion of glyoxylic acid to malic acid.
摘要翻译：本发明涉及一种生产二羧酸的方法，其中真核细胞在合适的发酵培养基中发酵。本发明进一步涉及包含编码催化异柠檬酸向琥珀酸的转化的酶的核苷酸序列的真核细胞和编码催化乙醛酸向苹果酸转化的酶的核苷酸序列。

3. 发明申请

WO2009113853A2 ADIPATE (ESTER OR THIOESTER) SYNTHESIS 审中-公开
标题翻译：综合（ESTER或THIOESTER）合成
公开(公告)号：WO2009113853A2
公开(公告)日：2009-09-17
申请号：PCT/NL2009050115
申请日：2009-03-11
申请人： DSM IP ASSETS BV , WU LIANG , TREFZER AXEL CHRISTOPH , DE WILDEMAN STEFAAN MARIE ANDR , VAN DEN BERG MARCO ALEXANDER
发明人： WU LIANG , TREFZER AXEL CHRISTOPH , DE WILDEMAN STEFAAN MARIE ANDRE , VAN DEN BERG MARCO ALEXANDER
IPC分类号： C12P7/44
CPC分类号： C12P7/44 , C12N15/70 , C12P7/62 , C12P17/10
摘要： The present invention relates to a method for preparing an adipate ester or thioester. The invention further relates to a method for preparing adipic acid from said ester or thioester. Further the invention provides a number of methods for preparing an intermediate for said ester or thioester. Further the invention relates to a method for preparing 6-amino caproic acid(6-ACA), a method for preparing 5-formyl valeric acid (5- FVA), and a method for preparingcaprolactam. Further, the invention relates to a host cell for use in a method according to the invention.
摘要翻译：本发明涉及一种制备己二酸酯或硫酯的方法。本发明还涉及从所述酯或硫酯制备己二酸的方法。此外，本发明提供了许多用于制备所述酯或硫酯的中间体的方法。本发明还涉及一种制备6-氨基己酸（6-ACA）的方法，5-甲酰戊酸（5-FVA）的制备方法及其制备己内酰胺的方法。此外，本发明涉及用于根据本发明的方法的宿主细胞。

4. 发明申请

WO2012167430A1 RECEIVER AND METHODS FOR PROVIDING CHANNEL STABILITY 审中-公开
标题翻译：接收机和提供信道稳定性的方法
公开(公告)号：WO2012167430A1
公开(公告)日：2012-12-13
申请号：PCT/CN2011/075495
申请日：2011-06-09
申请人： SILICON LABORATORIES, INC. , ZHANG, Ligang , WU, Liang , RONG, Jun
发明人： ZHANG, Ligang , WU, Liang , RONG, Jun
IPC分类号： H04B1/16
CPC分类号： H03J1/0008
摘要： A receiver includes an analog-to-digital converter (ADC) including an input configurable to digitize a voltage from a potentiometer and including an output for providing an ADC output signal. The receiver further includes a controller coupled to the output of the ADC and adapted to determine a selected channel based on multiple consecutive samples of the ADC output signal.
摘要翻译：接收器包括模数转换器（ADC），其包括可配置为将来自电位计的电压数字化并包括用于提供ADC输出信号的输出的输入。所述接收器还包括耦合到所述ADC的输出并适于基于所述ADC输出信号的多个连续采样来确定所选择的信道的控制器。

5. 发明申请

WO2009113853A3 ADIPATE ESTER OR THIOESTER SYNTHESIS 审中-公开
公开(公告)号：WO2009113853A3
公开(公告)日：2009-09-17
申请号：PCT/NL2009/050115
申请日：2009-03-11
申请人： DSM IP ASSETS B.V. , WU, Liang , TREFZER, Axel, Christoph , DE WILDEMAN, Stefaan, Marie, André , VAN DEN BERG, Marco, Alexander
发明人： WU, Liang , TREFZER, Axel, Christoph , DE WILDEMAN, Stefaan, Marie, André , VAN DEN BERG, Marco, Alexander
IPC分类号： C12P7/44
摘要： The present invention relates to a method for preparing an adipate ester or thioester. The invention further relates to a method for preparing adipic acid from said ester or thioester. Further the invention provides a number of methods for preparing an intermediate for said ester or thioester. Further the invention relates to a method for preparing 6-amino caproic acid(6-ACA), a method for preparing 5-formyl valeric acid (5- FVA), and a method for preparingcaprolactam. Further, the invention relates to a host cell for use in a method according to the invention.

6. 发明申请

WO2009013159A2 ACETYL-COA PRODUCING ENZYMES IN YEAST 审中-公开
标题翻译：乙酰乙酸生产酶在酵母中的应用
公开(公告)号：WO2009013159A2
公开(公告)日：2009-01-29
申请号：PCT/EP2008059119
申请日：2008-07-11
申请人： DSM IP ASSETS BV , MUELLER ULRIKE MARIA , WU LIANG , RAAMSDONK LOURINA MADELEINE , WINKLER AARON ADRIAAN
发明人： MUELLER ULRIKE MARIA , WU LIANG , RAAMSDONK LOURINA MADELEINE , WINKLER AARON ADRIAAN
IPC分类号： C12N15/81 , C12P7/16
CPC分类号： C12Q1/32 , C07K14/33 , C12N9/001 , C12P7/16 , Y02E50/10
摘要： The present invention relates to a method of identifying a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) a yeast cell comprising: a) providing a mutated yeast cell comprising a deletion of at least one gene of the (PDH) by-pass, selected from the genes encoding the enzymes pyruvate decarboxylase (PDC), acetaldehyde dehydrogenase (ALD), and acetyl-CoA synthetase (ACS); b) transforming said mutated yeast cell with an expression vector comprising a heterologous nucleotide sequence encoding a candidate polypeptide having potential enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA; c) testing said recombinant mutated yeast cell for its ability to grow on minimal medium containing glucose as sole carbon source, and d) identifying said candidate polypeptide as a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) said yeast cell when growth of said cell is observed. The invention further relates to a method of producing a fermentation production such as butanol.
摘要翻译：本发明涉及鉴定具有酶促活性的异源多肽的方法，所述酶促活性用于将丙酮酸，乙醛或乙酸转化为酵母细胞（的胞质溶胶）中的乙酰-CoA，所述方法包括：a）提供突变的酵母细胞，所述突变的酵母细胞包含至少选自编码丙酮酸脱羧酶（PDC），乙醛脱氢酶（ALD）和乙酰-CoA合成酶（ACS）的基因的（PDH）旁路的一个基因; b）用表达载体转化所述突变的酵母细胞，所述表达载体包含编码具有用于将丙酮酸，乙醛或乙酸转化成乙酰-CoA的潜在酶活性的候选多肽的异源核苷酸序列; c）测试所述重组突变的酵母细胞在含有葡萄糖作为唯一碳源的基本培养基上生长的能力，和d）将所述候选多肽鉴定为具有用于将丙酮酸，乙醛或乙酸转化为乙酰辅酶A的酶活性的异源多肽当观察到所述细胞的生长时，所述酵母细胞的胞质溶胶）。本发明进一步涉及生产发酵生产如丁醇的方法。

7. 发明申请

WO2011009859A1 PROCESS FOR THE PREPARATION OF 1,4-BUTANEDIAMINE VIA N-ACYL OR N-GUANIDYL PROTECTED 1,4-BUTANEDIAMINE PRECURSORS 审中-公开
标题翻译：通过N-乙酰基或N-胍基保护的1,4-丁二烯前体制备1,4-丁二胺的方法
公开(公告)号：WO2011009859A1
公开(公告)日：2011-01-27
申请号：PCT/EP2010/060480
申请日：2010-07-20
申请人： DSM IP ASSETS B.V. , WU, Liang , RAEMAKERS-FRANKEN, Petronella, Catharina
发明人： WU, Liang , RAEMAKERS-FRANKEN, Petronella, Catharina
IPC分类号： C12P13/00
CPC分类号： C12P13/001
摘要： The present invention relates to a novel method for the preparation of 1,4-diaminobutane [DAB]. The method according to the present invention involves at least one biocatalytic step which comprises the biocatalytic production of at least one N-protected precursor of DAB. The present invention also relates to a method for the preparation of DAB involving at least one biocatalytic step, and comprising the steps of a) biocatalytically preparing an N-protected precursor of DAB yielding a - biocatalytic reaction mixture containing the N-protected precursor of DAB, b) recovering the N-protected precursor from the biocatalytic reaction mixture and c) converting the N-protected precursor into DAB. More in particular, the present invention relates to a method for the preparation of DAB, wherein the at least N-protected precursor of DAB is selected from the group consisting of N5-protected ornithine, N-protected DAB, and N-protected 4-aminobutyraldehyde.
摘要翻译：本发明涉及一种制备1,4-二氨基丁烷[DAB]的新方法。根据本发明的方法涉及至少一种生物催化步骤，其包括至少一种N-保护的DAB前体的生物催化产生。本发明还涉及一种用于制备涉及至少一个生物催化步骤的DAB的方法，并且包括以下步骤：a）生物催化制备D受体的N-保护的前体，得到含有N-保护的DAB前体的生物催化反应混合物 b）从生物催化反应混合物回收N-保护的前体，和c）将N-保护的前体转化成DAB。更具体地说，本发明涉及一种制备DAB的方法，其中DAB的至少N-保护的前体选自N5保护的鸟氨酸，N-保护的DAB和N-保护的4- 氨基丁。

8. 发明申请

WO2010102982A1 METHOD FOR IMPROVING THE YIELD OF A POLYPEPTIDE 审中-公开
标题翻译：改善多肽的含量的方法
公开(公告)号：WO2010102982A1
公开(公告)日：2010-09-16
申请号：PCT/EP2010/052918
申请日：2010-03-08
申请人： DSM IP Assets B.V. , LAAN, VAN DER, Jan Metske , WU, Liang , ROUBOS, Johannes, Andries , PARENICOVA, Lucie , LOS, Alrik Pieter , PEIJ, VAN, Noël Nicolaas Maria Elisabeth , PEL, Herman Jan
发明人： LAAN, VAN DER, Jan Metske , WU, Liang , ROUBOS, Johannes, Andries , PARENICOVA, Lucie , LOS, Alrik Pieter , PEIJ, VAN, Noël Nicolaas Maria Elisabeth , PEL, Herman Jan
IPC分类号： C12P21/02 , C12N15/67
CPC分类号： C12P21/02
摘要： The present invention relates to a method for improving protein yield. The method comprises modifying the value of a set of relevant protein features to fall within an optimal range or to become more close to an optimal value for one or more protein features in the eukaryotic host.
摘要翻译：本发明涉及提高蛋白质产量的方法。该方法包括将一组相关蛋白质特征的值修改为落入最佳范围内，或者变得更接近于真核宿主中的一种或多种蛋白质特征的最佳值。

9. 发明申请

WO2010031772A2 ALTERNATIVE BUTANOL PRODUCTION PROCESS IN A MICROBIAL CELL 审中-公开
标题翻译：一种微生物细胞中的替代丁醇生产工艺
公开(公告)号：WO2010031772A2
公开(公告)日：2010-03-25
申请号：PCT/EP2009/061961
申请日：2009-09-15
申请人： DSM IP ASSETS B.V. , WU, Liang , PERKINS, John B. , SCHYNS, Ghislain
发明人： WU, Liang , PERKINS, John B. , SCHYNS, Ghislain
IPC分类号： C12N15/53 , C12N15/54 , C12N9/02 , C12N9/04 , C12N9/10 , C12P7/16
CPC分类号： C12N9/0006 , C12N9/0008 , C12N9/0051 , C12N9/1022 , C12N9/1029 , C12P7/16 , Y02E50/10
摘要： The present invention relates to a microbial cell comprising genes encoding enzymes that catalyse the following reactions a) pyruvate to acetolactate, b) acetolactate to 2,3-dihydroxyisovalerate, c) 2,3-dihydroxyisovalerate to 2-ketoisovalerate, d) 2- ketoisovalerate to isobutyryl-CoA, e) isobutyryl-CoA to butyryl-CoA, f) butyryl-CoA to butyraldehyde, and g) butyraldehyde to butanol, wherein the cell produces butanol. The invention further relates to a process for the production of butanol wherein the microbial cell according to the present invention is used.
摘要翻译：本发明涉及包含编码酶的基因的微生物细胞，所述酶催化下列反应a）丙酮酸转化为乙酰乳酸，b）乙酰乳酸转化为2,3-二羟基异戊酸，c）2,3-二羟基异戊酸转化成2 d）2-酮异戊酸酯与异丁酰-CoA，e）异丁酰-CoA与丁酰-CoA，f）丁酰-CoA与丁醛，和g）丁醛与丁醇，其中所述细胞产生丁醇。本发明还涉及生产丁醇的方法，其中使用了根据本发明的微生物细胞。

10. 发明申请

WO2009013159A3 ACETYL-COA PRODUCING ENZYMES IN YEAST 审中-公开
公开(公告)号：WO2009013159A3
公开(公告)日：2009-01-29
申请号：PCT/EP2008/059119
申请日：2008-07-11
申请人： DSM IP ASSETS B.V. , MUELLER, Ulrike, Maria , WU, Liang , RAAMSDONK, Lourina, Madeleine , WINKLER, Aaron Adriaan
发明人： MUELLER, Ulrike, Maria , WU, Liang , RAAMSDONK, Lourina, Madeleine , WINKLER, Aaron Adriaan
IPC分类号： C12N15/81 , C12P7/16
摘要： The present invention relates to a method of identifying a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) a yeast cell comprising: a) providing a mutated yeast cell comprising a deletion of at least one gene of the (PDH) by-pass, selected from the genes encoding the enzymes pyruvate decarboxylase (PDC), acetaldehyde dehydrogenase (ALD), and acetyl-CoA synthetase (ACS); b) transforming said mutated yeast cell with an expression vector comprising a heterologous nucleotide sequence encoding a candidate polypeptide having potential enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA; c) testing said recombinant mutated yeast cell for its ability to grow on minimal medium containing glucose as sole carbon source, and d) identifying said candidate polypeptide as a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) said yeast cell when growth of said cell is observed. The invention further relates to a method of producing a fermentation production such as butanol.

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