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1. 发明申请

WO2015070157A1 USE OF ALDEHYDES TO ENHANCE DISEASE RESISTANCE OF PLANTS TO LIBERIBACTERS 审中-公开
标题翻译：使用ALDEHYDES以提高植物抗病性的耐药性
公开(公告)号：WO2015070157A1
公开(公告)日：2015-05-14
申请号：PCT/US2014/064835
申请日：2014-11-10
申请人： UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC. , INTEGRATED PLANT GENETICS, INC.
发明人： GABRIEL, Dean W. , ZHANG, Shujian
IPC分类号： A01N35/00 , A01P5/00
CPC分类号： A01N35/02 , A01N25/02 , A01N31/08
摘要： Methods and compositions based upon using phenolic aromatic aldehydes (ex: einnamaldehyde, benzaldehyde) are provided, which find use as agents for treating, preventing, or curing systemic bacterial infections of living plants, in particular against Gram negative bacteria, and more particularly species of Liberibacter, including Cα . Liberibacter asiaticus. The agent compositions described are used synergistically with other antimicrobial compounds, such as those that plants manufacture or release as a result, of biotic or abiotic stresses, including the application of the aldehydes, proteins, whether produced by recombinant methods or not, or by essential oils such as carvacrol or allicin. Methods of applying the compositions for agriculture use are disclosed.
摘要翻译：提供了基于使用酚醛芳族醛（例如：欧内亚醛，苯甲醛）的方法和组合物，其可用作治疗，预防或治疗活的植物，特别是抗革兰氏阴性细菌的全身性细菌感染的试剂，更具体地，自由基，包括Cα。免疫杆菌。所描述的试剂组合物与其它抗微生物化合物协同使用，例如植物生产或释放生物或非生物胁迫的抗微生物化合物，包括施用醛，蛋白质，无论是否通过重组方法产生，或必需的油如香芹酚或大蒜素。公开了应用农业用组合物的方法。

2. 发明申请

WO2007050715A2 COMPOSITIONS AND METHODS FOR SAFE DELIVERY OF BIOLOGICALLY-ACTIVE PLANT TRANSFORMATION AGENTS USING NON-FIBROUS SILICON CARBIDE POWDER 审中-公开
标题翻译：使用非碳化硅碳粉安全输送生物活性植物转化剂的组合物和方法
公开(公告)号：WO2007050715A2
公开(公告)日：2007-05-03
申请号：PCT/US2006/041702
申请日：2006-10-26
申请人： INTEGRATED PLANT GENETICS, INC. , REDDY, Joseph, D. , GABRIEL, Dean, W.
发明人： REDDY, Joseph, D. , GABRIEL, Dean, W.
IPC分类号： A01H5/00
CPC分类号： C12N15/8207
摘要： According to the described invention, a biologically-active nucleic acid may be directly delivered into plant cells in an improved and operator-safe fashion using encapsulated or complexed nucleic acid formed into nanoparticles, using carborundum powder, a nucleic acid binding protein with nuclear localization signals (NLSs), or both. The plant cells are not enzymatically treated, and the delivery is not mediated by any microbial agent. The present invention provides the following: (1) a nucleic acid delivery system (NADS), i.e. macromolecular nanoparticles to impart the novel conditions; (2) a novel carrier medium (CM) used with the nucleic acid delivery system which impart the desired results; (3) novel, specific conditions that impart the desired or improved efficiency of the transformation system into the plant cell without irreversibly damaging it, and (4) a novel method to produce these conditions through the use of a combination of DNA condensing agents, carborundum powder, a nucleic acid binding protein with NLSs, which together imparts improved nucleic acid delivery efficiency and safety as compared to other known systems.
摘要翻译：根据所述发明，使用碳化硅粉末，具有核定位信号的核酸结合蛋白，可以以改进和操作者安全的方式将生物活性核酸直接递送至植物细胞中，使用形成纳米颗粒的封装或复合核酸（NLS），或两者兼而有之。植物细胞不经酶处理，并且递送不被任何微生物剂介导。本发明提供以下：（1）核酸递送系统（NADS），即赋予新条件的大分子纳米粒子; （2）与赋予所需结果的核酸递送系统一起使用的新型载体介质（CM）（3）将转化体系的期望的或提高的效率赋予植物细胞而不会不可逆地损害其的新的具体条件，以及（4）通过使用DNA缩合剂，金刚砂的组合产生这些条件的新方法粉末，具有NLS的核酸结合蛋白，与其它已知系统相比，它们一起提供了改善的核酸递送效率和安全性。

3. 发明申请

WO2020047294A1 METHODS AND COMPOSITIONS USING RNAi FOR PREVENTING OR REDUCING INFECTIONS OF CROP PLANTS BY BIOTROPHIC PATHOGENS 审中-公开
公开(公告)号：WO2020047294A1
公开(公告)日：2020-03-05
申请号：PCT/US2019/048870
申请日：2019-08-29
申请人： INTEGRATED PLANT GENETICS, INC. , UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.
发明人： STRAUSS, Tina , GABRIEL, Dean
IPC分类号： A01H5/00 , A61K31/713 , C07K14/415 , C12N15/11 , C12N15/113 , C12N15/29
摘要： The present invention teaches methods and compositions useful for treating, preventing, or curing pathogen infections of living plants. In particular, the present invention teaches methods of enhancing plant response to pathogen-associated molecular patterns using RNA interference of plant genes. The methods and compositions described herein are effective at treating biotrophic pathogens, including Liberibacters.

4. 发明申请

WO2020006393A1 MENADIONE COMPOSITIONS FOR TREATING PLANT DISEASES IN GRAPE PLANTS AND CITRUS 审中-公开
公开(公告)号：WO2020006393A1
公开(公告)日：2020-01-02
申请号：PCT/US2019/039793
申请日：2019-06-28
申请人： UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC. , INTEGRATED PLANT GENETICS, INC.
发明人： GABRIEL, Dean, W. , JAIN, Mukesh , ZHANG, Shujian , STRAUSS, Tina
IPC分类号： A61K31/00
摘要： Disclosed herein are methods of treating or improving resistance against Pierce's disease in Vitis vinifera via administration of a menadione containing composition. Also disclosed are methods of treating citrus exhibiting symptoms of Huanglongbing disease.

5. 发明申请

WO2009012481A1 USE OF BACTERIOPHAGE OUTER MEMBRANE BREACHING PROTEINS EXPRESSED IN PLANTS FOR THE CONTROL OF GRAM-NEGATIVE BACTERIA 审中-公开
标题翻译：在植物中表达的细菌外膜促进蛋白的使用用于控制噬菌体
公开(公告)号：WO2009012481A1
公开(公告)日：2009-01-22
申请号：PCT/US2008/070612
申请日：2008-07-21
申请人： INTEGRATED PLANT GENETICS, INC. , UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC. , REDDY, Joseph D. , GABRIEL, Dean W.
发明人： REDDY, Joseph D. , GABRIEL, Dean W.
IPC分类号： C07H21/04 , A01H5/00 , C12N15/31 , C12N15/82
CPC分类号： C12N15/8281 , A01N63/02 , A61K38/162 , C07K14/005 , C12N7/00 , C12N2710/00022
摘要： The present invention provides compositions and methods for killing or suppressing growth of Gram-negative bacteria that infect, infest or cause disease in plants, including pathogenic, saprophytic and opportunistic microbes that cause disease in plants and food borne illness in people or in animal feed.
摘要翻译：本发明提供用于杀死或抑制在植物中感染，感染或引起疾病的革兰氏阴性细菌的生长的组合物和方法，包括致病，腐植酸和机会性微生物，其引起植物中的疾病和人或动物饲料中的食源性疾病。

6. 发明申请

WO2008030858A2 USE OF ESTERASE EXPRESSED IN PLANTS FOR THE CONTROL OF GRAM-NEGATIVE BACTERIA 审中-公开
标题翻译：在植物中表达用于控制GRA阴性细菌的ESTERASE的使用
公开(公告)号：WO2008030858A2
公开(公告)日：2008-03-13
申请号：PCT/US2007/077614
申请日：2007-09-05
申请人： INTEGRATED PLANT GENETICS, INC. , GABRIEL, Dean W. , REDDY, Joseph D.
发明人： GABRIEL, Dean W. , REDDY, Joseph D.
IPC分类号： A01H5/00
CPC分类号： C12N15/8281 , A01N63/00 , C12N9/16 , C12N9/18 , C12N9/20 , C12N15/8257 , C12N15/8266
摘要： The present invention provides various methods of using esterase to control gram-negative bacteria. The present invention also provides transgenic plants expressing esterase and methods of their production and use. The present invention finds applications in many fields, including plant husbandry, water purification, food safety, bioterrorism prevention, and cut flower maintenance.
摘要翻译：本发明提供了使用酯酶控制革兰氏阴性细菌的各种方法。本发明还提供了表达酯酶的转基因植物及其生产和使用方法。本发明在植物养殖，净水，食品安全，防止生物恐怖，切花保养等领域得到应用。

7. 发明申请

WO2004104169A2 IDENTIFICATION AND USE OF GENES ENCODING HOLINS AND HOLIN-LIKE PROTEINS IN PLANTS FOR THE CONTROL OF MICROBES AND PESTS 审中-公开
标题翻译：识别和使用在植物中编织HOLINS和HOLIN-like蛋白质的基因用于控制微生物和细菌
公开(公告)号：WO2004104169A2
公开(公告)日：2004-12-02
申请号：PCT/US2004015099
申请日：2004-05-14
申请人： INTEGRATED PLANT GENETICS INC , UNIV FLORIDA , RAMADUGU CHANDRIKA , GABRIEL DEAN WILLIAM
发明人： RAMADUGU CHANDRIKA , GABRIEL DEAN WILLIAM
IPC分类号： C07K14/01 , C12N20060101 , C12N15/82 , C12N
CPC分类号： C07K14/005 , C12N15/8279 , C12N2795/10122
摘要： This invention provides: 1) methods for the identification of broad-spectrum holins with a high level of nonenzymatic activity in membranes; 2) conditions required for maintaining and increasing the anti-microbial and anti-pest efficacy of holins in gene fusions; 3) a method for effective targeting of holins expressed in plants through use of a leader peptide to direct the holin protein to the plant apoplast and xylem; 4) methods for the control of bacterial and fungal diseases of plants and control of insect and nematode pests that attack plants by expression of gene fusions involving holins, C-terminal additions and leader peptides, and optionally, endolysins; 5) methods for increasing the shelf-life of cut flowers, and 6) transgenic plants useful for the production of novel antimicrobial proteins based upon holins.
摘要翻译：本发明提供：1）用于鉴定膜中非酶活性高水平的广谱凝胶的方法; 2）保持和提高基因融合中的蛋白的抗微生物和抗虫功效所需的条件; 3）通过使用前导肽将植物蛋白质引导到植物质外体和木质部来有效靶向在植物中表达的蛋白的方法; 4）用于控制植物细菌和真菌疾病的方法，以及通过表达涉及凝集素，C-末端添加物和前导肽以及任选的细胞内溶素的基因融合来攻击植物的昆虫和线虫害虫的控制方法; 5）提高切花保质期的方法，以及6）可用于生产基于蛋白质的新型抗微生物蛋白质的转基因植物。

8. 发明申请

WO1990000002A1 METHOD FOR MATURING SOMATIC EMBRYOS FOR PLANTING IN NATURAL ENVIRONMENTS 审中-公开
标题翻译：用于生产用于自然环境中的植物的方法
公开(公告)号：WO1990000002A1
公开(公告)日：1990-01-11
申请号：PCT/US1989002562
申请日：1989-06-13
申请人： PLANT GENETICS, INC. , KIRIN BEER KABUSHIKI KAISHA
发明人： PLANT GENETICS, INC. , KIRIN BEER KABUSHIKI KAISHA , FUJII, Jo, Ann , SLADE, David , REDENBAUGH, M., Keith
IPC分类号： A01H01/00
CPC分类号： A01H4/006
摘要： A novel method is provided for producing plants from somatic embryos which do not require an in vitro planting environment. Mature, tissue culture-derived somatic embryos are produced which display hardiness similar to true seed (zygotic) embryos and which respond similarly when planted in typical seed environments.
摘要翻译：提供了一种用于从不需要体外种植环境的体细胞胚产生植物的新方法。产生成熟的组织培养来源的体细胞胚，其表现出与真实种子（合子）胚胎相似的耐受性，并且当在典型的种子环境中种植时，它们的反应类似。

9. 发明申请

WO2015143444A1 USE OF ALDEHYDES FORMULATED WITH NANOPARTICLES AND/OR NANOEMULSIONS TO ENHANCE DISEASE RESISTANCE OF PLANTS TO LIBERIBACTERS 审中-公开
标题翻译：使用纳米颗粒和/或纳米粒子配制的醛类，以提高植物对耐药性的抗病能力
公开(公告)号：WO2015143444A1
公开(公告)日：2015-09-24
申请号：PCT/US2015/022046
申请日：2015-03-23
申请人： UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC. , INTEGRATED PLANT GENETICS, INC.
发明人： GABRIEL, Dean W. , ZHANG, Shujian
IPC分类号： A01N35/04
CPC分类号： A01N35/02 , A01N25/02 , A01N25/04 , A01N31/02 , A01N59/16
摘要： Methods and compositions based upon natural phenolic aromatic aldehydes (ex: cinnamaldehyde, benzaldehyde) are provided, which find use as curing agents for systemic bacterial infections of living plants, in particular against Gram negative bacteria and more particularly species of Liberibacter, including Ca. Liberibacter asiaticus. The curing agent compositions described are used synergistically with other antimicrobial compounds, such as those that plants manufacture or release as a result of biotic or abiotic stresses, including the application of the subject aldehydes, certain proteins, whether produced by recombinant methods or not, nanoparticles, nanoemulsions, or by natural essential oils such as carvacrol or allicin. The aromatic aldehydes have high volatility and exhibit phytotoxicity when applied above certain amounts. Systemic infections and infestations of a variety of plant parts can be treated, including those of leaves and roots. Methods of applying the compositions for agriculture use are disclosed.
摘要翻译：提供了基于天然酚醛芳族醛（例如：肉桂醛，苯甲醛）的方法和组合物，其可用作活体植物的系统性细菌感染的固化剂，特别是用于革兰氏阴性细菌，更特别是包括Ca的Liberibacter种。免疫杆菌。所描述的固化剂组合物与其它抗微生物化合物协同使用，例如由于生物或非生物胁迫而产生或释放植物的化合物，包括施用受试者醛，某些蛋白质，无论是否通过重组方法产生的纳米颗粒，纳米乳剂，或天然精油如香芹酚或大蒜素。芳香醛具有高挥发性，当超过一定量时具有植物毒性。可以治疗各种植物部位的全身感染和侵染，包括叶和根的感染和侵染。公开了应用农业用组合物的方法。

10. 发明申请

WO2013032985A1 TRANSFORMATION OF JUVENILE AND MATURE CITRUS 审中-公开
标题翻译：转基因和成熟CITRUS
公开(公告)号：WO2013032985A1
公开(公告)日：2013-03-07
申请号：PCT/US2012/052452
申请日：2012-08-27
申请人： INTEGRATED PLANT GENETICS, INC. , GABRIEL, Dean, W. , PERAZZO-RATTO, Giovana, J. , YINGNAN, Jiang
发明人： GABRIEL, Dean, W. , PERAZZO-RATTO, Giovana, J. , YINGNAN, Jiang
IPC分类号： C12N15/82 , C12N15/87 , A01H5/00
CPC分类号： A01H4/001 , A01H4/005 , C12N15/8202
摘要： The invention relates to methods and compositions for genetic transformation of both juvenile and mature citrus. In some embodiments, the invention provides methods and compositions for genetic transformation of citrus using Rhizobia-mediated DNA delivery, and also methods of enhancing the frequency of genetic transformation of mature citrus by any DNA transfer method, including Sinorhizobium . Internodal stem sections prepared from epicotyls of citrus seedlings or freshly emerging shoots of mature citrus plants (e.g., first shoots from buds of mature plants following grafting onto rootstock or very young shoots of mature plants) are preconditioned for transformation by inducing callus formation on an artificial medium. All callus and any developing meristematic regions in immediately adjacent tissue are substantially or completely removed and the preconditioned explants are then transformed by Sinorhizobium or other known methods. Whole plants or shoots to be grafted onto rootstocks are subsequently regenerated from the transformed cells.
摘要翻译：本发明涉及幼体和成熟柑橘的遗传转化的方法和组合物。在一些实施方案中，本发明提供使用根瘤菌介导的DNA递送的柑橘遗传转化的方法和组合物，以及通过任何DNA转移方法（包括中华根瘤菌）提高成熟柑橘的遗传转化的频率的方法。由柑橘幼苗的上胚叶或成熟柑橘植物的新鲜出芽（例如，成熟植物的萌芽后的成熟植物的萌芽的第一枝，在砧木或成熟植物的非常幼嫩的芽后）制备的节间茎部分通过诱导人造丝的愈伤组织形成来预处理中。所有愈伤组织和立即相邻组织中的任何发育中的分生组织基本上或完全去除，然后用Sinorhizobium或其他已知方法转化预处理的外植体。随后从转化细胞中再生整个植物或嫁接到砧木上的枝条。

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