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1. 发明申请

WO2013032985A1 TRANSFORMATION OF JUVENILE AND MATURE CITRUS 审中-公开
标题翻译：转基因和成熟CITRUS
公开(公告)号：WO2013032985A1
公开(公告)日：2013-03-07
申请号：PCT/US2012/052452
申请日：2012-08-27
申请人： INTEGRATED PLANT GENETICS, INC. , GABRIEL, Dean, W. , PERAZZO-RATTO, Giovana, J. , YINGNAN, Jiang
发明人： GABRIEL, Dean, W. , PERAZZO-RATTO, Giovana, J. , YINGNAN, Jiang
IPC分类号： C12N15/82 , C12N15/87 , A01H5/00
CPC分类号： A01H4/001 , A01H4/005 , C12N15/8202
摘要： The invention relates to methods and compositions for genetic transformation of both juvenile and mature citrus. In some embodiments, the invention provides methods and compositions for genetic transformation of citrus using Rhizobia-mediated DNA delivery, and also methods of enhancing the frequency of genetic transformation of mature citrus by any DNA transfer method, including Sinorhizobium . Internodal stem sections prepared from epicotyls of citrus seedlings or freshly emerging shoots of mature citrus plants (e.g., first shoots from buds of mature plants following grafting onto rootstock or very young shoots of mature plants) are preconditioned for transformation by inducing callus formation on an artificial medium. All callus and any developing meristematic regions in immediately adjacent tissue are substantially or completely removed and the preconditioned explants are then transformed by Sinorhizobium or other known methods. Whole plants or shoots to be grafted onto rootstocks are subsequently regenerated from the transformed cells.
摘要翻译：本发明涉及幼体和成熟柑橘的遗传转化的方法和组合物。在一些实施方案中，本发明提供使用根瘤菌介导的DNA递送的柑橘遗传转化的方法和组合物，以及通过任何DNA转移方法（包括中华根瘤菌）提高成熟柑橘的遗传转化的频率的方法。由柑橘幼苗的上胚叶或成熟柑橘植物的新鲜出芽（例如，成熟植物的萌芽后的成熟植物的萌芽的第一枝，在砧木或成熟植物的非常幼嫩的芽后）制备的节间茎部分通过诱导人造丝的愈伤组织形成来预处理中。所有愈伤组织和立即相邻组织中的任何发育中的分生组织基本上或完全去除，然后用Sinorhizobium或其他已知方法转化预处理的外植体。随后从转化细胞中再生整个植物或嫁接到砧木上的枝条。

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