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1. 发明申请

WO2012049491A2 ANALYTE EXTRACTION APPARATUS AND METHOD 审中-公开
标题翻译：分析物萃取装置和方法
公开(公告)号：WO2012049491A2
公开(公告)日：2012-04-19
申请号：PCT/GB2011/051958
申请日：2011-10-11
申请人： SPHERE MEDICAL LIMITED , PETTIGREW, David Michael , LIU, Bo , LAITENBERGER, Peter Georg , BATES, Stephen
发明人： PETTIGREW, David Michael , LIU, Bo , LAITENBERGER, Peter Georg , BATES, Stephen
IPC分类号： G01N30/06
CPC分类号： G01N33/948 , G01N30/06 , G01N30/24 , G01N2030/067 , G01N2030/085 , Y10T436/11 , Y10T436/203332 , Y10T436/255
摘要： Disclosed is an apparatus for automatically extracting i2,6-diisopropylphenol (Propofol), from a complex sample matrix comprising cellular material, e.g. blood, the apparatus comprising a sample reception stage having on output for providing a defined quantity of the complex sample matrix; a mixing stage having a first input in fluidic connection with the output of the sample reception stage, a second input for receiving a lysing agent and an output for providing a mixture of the defined quantity of the complex sample matrix and the lysing agent; a delay stage having an input in fluidic connection with the output of the mixing stage and an output for providing the delayed mixture of the defined quantity of the complex sample matrix and the lysing agent; a filtering stage comprising a sorbent material for mechanically lysing the cellular material, said sorbent material having an affinity for binding Propofol, said filtering stage having an input in fluidic connection with the output of the delay stage; and a controller for controlling the flow rate of the mixture of the defined quantity of the complex sample matrix and the lysing agent through said delay stage. A method for such extraction is also disclosed.
摘要翻译：本发明公开了一种用于从包含细胞材料的复杂样品基质（例如细胞材料）自动提取1,2,6-二异丙基苯酚（丙泊酚）的装置。所述设备包括：样本接收级，其具有用于提供限定量的所述复杂样本矩阵的输出; 混合阶段，其具有与样品接收阶段的输出物流体连接的第一输入端，用于接收裂解剂的第二输入端和用于提供限定量的复杂样品基质和裂解剂的混合物的输出端; 延迟级，其具有与混合级的输出端流体连接的输入端和用于提供限定量的复杂样品基质和裂解剂的延迟混合物的输出端; 包含用于机械裂解所述细胞材料的吸附剂材料的过滤阶段，所述吸附剂材料具有与丙泊酚结合的亲和力，所述过滤阶段具有与所述延迟阶段的输出端流体连接的输入端; 以及控制器，用于通过所述延迟阶段控制限定量的复杂样品基质和裂解剂的混合物的流速。还公开了这种提取的方法。

2. 发明申请

WO2012049486A1 ANALYTE DETECTION METHOD 审中-公开
标题翻译：分析检测方法
公开(公告)号：WO2012049486A1
公开(公告)日：2012-04-19
申请号：PCT/GB2011/051952
申请日：2011-10-11
申请人： SPHERE MEDICAL LIMITED , PETTIGREW, David Michael , LAITENBERGER, Peter Georg , LIU, Bo
发明人： PETTIGREW, David Michael , LAITENBERGER, Peter Georg , LIU, Bo
IPC分类号： G01N33/52 , G01N33/557 , G01N33/94
CPC分类号： G01N33/948 , G01N33/52 , G01N33/557 , Y10T436/14 , Y10T436/203332
摘要： Disclosed is a method of determining the presence of an analyte of interest by means of a detection of a reaction product between the analyte of interest and a reactant, the method comprising extracting the analyte of interest from a complex sample matrix; transferring the analyte of interest to an initial reaction mixture; performing a background measurement on the initial reaction mixture comprising at most a negligible concentration of the reaction product, wherein the reaction conditions present in said initial reaction mixture at least reduce the reaction rate of the formation of the reaction product such that the background measurement can be performed without a measurable change in said negligible concentration; altering the reaction conditions in the initial reaction mixture to accelerate said reaction rate; continuing said reaction until the concentration of the reaction product has stabilized; performing a second measurement on the resultant reaction mixture to obtain a signal correlated to said concentration; and determining the presence of the analyte of interest from a difference between the background measurement and the second measurement. In a preferred embodiment, the analyte of interest is Propofol (2,6-di-isopropylphenol), and the reactant is the activated Gibbs reagent (2,6-dichloroquinoneimine).
摘要翻译：公开了通过检测目标分析物和反应物之间的反应产物来确定感兴趣分析物的存在的方法，该方法包括从复杂样品基质提取目的分析物; 将目标分析物转移到初始反应混合物中; 对起始反应混合物进行背景测量，该反应混合物至少包含反应产物的可忽略的浓度，其中存在于所述初始反应混合物中的反应条件至少降低反应产物形成的反应速率，使得背景测量可以在所述可忽略的浓度中没有可测量的变化; 改变初始反应混合物中的反应条件以加速所述反应速率; 继续反应直到反应产物的浓度稳定; 对所得反应混合物进行第二次测定以获得与所述浓度相关的信号; 以及从背景测量和第二测量之间的差异确定感兴趣分析物的存在。在优选的实施方案中，感兴趣的分析物是异丙酚（2,6-二异丙基苯酚），反应物是活化的吉布斯试剂（2,6-二氯喹啉亚胺）。

3. 发明申请

WO2012049491A3 ANALYTE EXTRACTION APPARATUS AND METHOD 审中-公开
标题翻译：分析提取装置和方法
公开(公告)号：WO2012049491A3
公开(公告)日：2012-06-07
申请号：PCT/GB2011051958
申请日：2011-10-11
申请人： SPHERE MEDICAL LTD , PETTIGREW DAVID MICHAEL , LIU BO , LAITENBERGER PETER GEORG , BATES STEPHEN
发明人： PETTIGREW DAVID MICHAEL , LIU BO , LAITENBERGER PETER GEORG , BATES STEPHEN
IPC分类号： G01N30/06 , B01D11/02 , G01N33/15
CPC分类号： G01N33/948 , G01N30/06 , G01N30/24 , G01N2030/067 , G01N2030/085 , Y10T436/11 , Y10T436/203332 , Y10T436/255
摘要： Disclosed is an apparatus for automatically extracting i2,6-diisopropylphenol (Propofol), from a complex sample matrix comprising cellular material, e.g. blood, the apparatus comprising a sample reception stage having on output for providing a defined quantity of the complex sample matrix; a mixing stage having a first input in fluidic connection with the output of the sample reception stage, a second input for receiving a lysing agent and an output for providing a mixture of the defined quantity of the complex sample matrix and the lysing agent; a delay stage having an input in fluidic connection with the output of the mixing stage and an output for providing the delayed mixture of the defined quantity of the complex sample matrix and the lysing agent; a filtering stage comprising a sorbent material for mechanically lysing the cellular material, said sorbent material having an affinity for binding Propofol, said filtering stage having an input in fluidic connection with the output of the delay stage; and a controller for controlling the flow rate of the mixture of the defined quantity of the complex sample matrix and the lysing agent through said delay stage. A method for such extraction is also disclosed.
摘要翻译：公开了一种用于从包含细胞材料的复杂样品基质中自动提取i2,6-二异丙基苯酚（异丙酚）的装置，例如，血液，所述装置包括具有用于提供限定量的所述复样本矩阵的输出的样本接收台; 混合阶段具有与样品接收台的输出流体连接的第一输入，用于接收裂解剂的第二输入和用于提供所定量的复合样品基质和裂解剂的混合物的输出; 具有与混合级的输出流体连接的输入的延迟级和用于提供限定量的复合样品基质和裂解剂的延迟混合物的输出; 过滤阶段包括用于机械裂解细胞材料的吸附剂材料，所述吸附剂材料具有对结合丙泊酚的亲和力，所述过滤级具有与延迟级的输出流体连接的输入; 以及控制器，用于通过所述延迟级来控制所述限定量的所述复合样本矩阵和所述裂解剂的混合物的流量。还公开了这种提取的方法。

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