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    • 2. 发明申请
    • PROTEASES
    • 蛋白酶
    • WO2004072221A3
    • 2004-09-30
    • PCT/DK2004000090
    • 2004-02-09
    • NOVOZYMES ASOESTERGAARD PETER RAHBEKMARIA LEONARDO DE
    • OESTERGAARD PETER RAHBEKMARIA LEONARDO DE
    • C12N9/52C12N9/58C12N15/57A23J3/34
    • C12N9/58A01K2217/05C12N9/52C12N2310/111
    • The invention relates to a novel class of serine proteases of peptidase family S2A or S1E that are stable in the presence of copper (Cu2+) and/or inhibited by copper only to a limited extent. Structural features of potential relevance for this effect are also disclosed. This class of proteases includes proteases derived from Brachysporiella gayana, Nocardiopsis dassonvillei subsp. dassonvillei, Nocardiopsis prasina, and Nocardiopsis alba, but excludes the known proteases derived from Metarhizium anisopliae and Nocardiopsis sp. NRRL 18262. The invention also relates to DNA encoding such proteases, the expression thereof in a host cell, includiThe invention relates to a novel class of serine proteases of peptidase family S2A or S1E that are stable in the presence of copper (Cu2+) and/or inhibited by copper only to a limited extent. Structural features of potential relevance for this effect are also disclosed. This class of proteases includes proteases derived from Brachysporiella gayana, Nocardiopsis dassonvillei subsp. dassonvillei, Nocardiopsis prasina, and Nocardiopsis alba, but excludes the known proteases derived from Metarhizium anisopliae and Nocardiopsis sp. NRRL 18262. The invention also relates to DNA encoding such proteases, the expression thereof in a host cell, including animal and plant cells, as well as to the use thereof, e.g. in animal feed and in detergents. In particular, the invention relates to animal feed and animal feed additives, such as premix, incorporating these proteases together with 1-500 ppm Cu (in-feed-concentration).
    • 本发明涉及一类新型的肽酶家族S2A或S1E的丝氨酸蛋白酶,其在铜(Cu 2+)存在下稳定和/或仅在有限程度上被铜抑制。 也披露了这种效应潜在相关性的结构特征。 这类蛋白酶包括源自Brachysporiella gayana,Nocardiopsis dassonvillei亚种的蛋白酶。 达松维尔,Nocardiopsis prasina和Nocardiopsis alba,但不包括来源于绿僵菌和拟诺卡氏菌属的已知蛋白酶。 NRRL 18262。本发明还涉及编码这种蛋白酶的DNA,其在宿主细胞中的表达,包括它们本发明涉及一类新型的肽酶家族S2A或S1E的丝氨酸蛋白酶,其在铜(Cu 2+)和/ 或仅在有限程度上被铜抑制。 也披露了这种效应潜在相关性的结构特征。 这类蛋白酶包括源自Brachysporiella gayana,Nocardiopsis dassonvillei亚种的蛋白酶。 达松维尔,Nocardiopsis prasina和Nocardiopsis alba,但不包括来源于绿僵菌和拟诺卡氏菌属的已知蛋白酶。 NRRL 18262。本发明还涉及编码这种蛋白酶的DNA,其在包括动物和植物细胞的宿主细胞中的表达,以及它们的用途,例如, 在动物饲料和洗涤剂中。 具体而言,本发明涉及动物饲料和动物饲料添加剂,例如预混合物,将这些蛋白酶与1-500ppm Cu(进料中浓度)一起加入。