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1. 发明申请

WO2004081205A1 DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS TO CARDIOMYOCYTES 审中-公开
标题翻译：人胚胎干细胞对心脏细胞的分化
公开(公告)号：WO2004081205A1
公开(公告)日：2004-09-23
申请号：PCT/AU2004/000302
申请日：2004-03-11
申请人： ES CELL INTERNATIONAL PTE LTD , NETHERLANDS INSTITUTE VOOR ONTWIKKELINGSBIOLOGIE , IP ORGANISERS PTY LTD , MUMMERY, Christine, Lindsay , DOEVENDANS, Petrus, Adrianus, Frederik, Maria , TERTOOLEN, Leon, Gerardus, Joseph
发明人： MUMMERY, Christine, Lindsay , DOEVENDANS, Petrus, Adrianus, Frederik, Maria , TERTOOLEN, Leon, Gerardus, Joseph
IPC分类号： C12N5/08
CPC分类号： C12N5/0657 , C12N2502/02 , C12N2506/02
摘要： A method for inducing cardiomyocyte differentiation of a hES cell, the method comprising co-culturing the hES cell with a cell excreting at least one cardiomyocyte differentiation inducing factor or with an extracellular medium therefrom, under conditions that induce differentiation, cells and cell populations so produced, and uses of the cells.
摘要翻译：一种用于诱导hES细胞的心肌细胞分化的方法，所述方法包括在诱导分化的条件，如此产生的细胞和细胞群的情况下，共同培养hES细胞与排泄至少一种心肌细胞分化诱导因子或与细胞外介质的细胞，以及细胞的使用。

2. 发明申请

WO2007070964A1 DIRECT DIFFERENTIATION OF CARDIOMYOCYTES FROM HUMAN EMBRYONIC STEM CELLS 审中-公开
标题翻译：来自人胚胎干细胞的心脏细胞的直接分化
公开(公告)号：WO2007070964A1
公开(公告)日：2007-06-28
申请号：PCT/AU2006/001969
申请日：2006-12-22
申请人： ES CELL INTERNATIONAL PTE LTD , IP ORGANISERS PTY LTD , DAVIDSON, Bruce, Paul , GRAICHEN, Ralph, Eberhard , ZWEIGERDT, Robert , XUIQIN, Xu , MUMMERY, Christine, Lindsay , SUN, William
发明人： DAVIDSON, Bruce, Paul , GRAICHEN, Ralph, Eberhard , ZWEIGERDT, Robert , XUIQIN, Xu , MUMMERY, Christine, Lindsay , SUN, William
IPC分类号： C12N5/08
CPC分类号： C12N5/0657 , A61K35/12 , C12N5/0043 , C12N2500/25 , C12N2500/99 , C12N2501/02 , C12N2501/105 , C12N2501/115 , C12N2501/125 , C12N2501/155 , C12N2501/33 , C12N2501/999 , C12N2502/13 , C12N2502/30 , C12N2506/02 , C12N2510/00 , G01N33/502 , G01N33/5061
摘要： The present invention relates to the induction of differentiation in stem cells to cardiomyocytes and factors such as prostaglandin alone or in combination with other factors including essential minerals selected from the group including transferrin and selenium, small molecules selected from the group including a p38 MAPK inhibitor such as SB203580 and protein growth factors of the FGF, IGF and BMP families such as but not limited to IGF1 , FGF2, BMP2, BMP4 and BMP6. and insulin that influence the process of differentiation to cardiomyocytes. Media that is appropriate for the induction of differentiation of cardiomyocytes from stem cells is also provided wherein the media contains these factors. The use of cardiomyocytes and cardiac progenitors produced by the directed differentiation in transplantation and screening for cardiac compounds is also provided.
摘要翻译：本发明涉及诱导干细胞分化为心肌细胞和诸如前列腺素的因素，或与其它因素组合，包括选自包括转铁蛋白和硒的必需矿物质，选自包括p38MAPK抑制剂的小分子作为SB203580和FGF，IGF和BMP家族的蛋白生长因子，例如但不限于IGF1，FGF2，BMP2，BMP4和BMP6。和影响心肌细胞分化过程的胰岛素。还提供了适合于诱导心肌细胞从干细胞分化的培养基，其中培养基含有这些因子。还提供了通过移植中的定向分化和心脏化合物筛选产生的心肌细胞和心脏祖细胞的使用。

3. 发明申请

WO2004081205A8 DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS TO CARDIOMYOCYTES 审中-公开
标题翻译：人胚胎干细胞向心肌细胞的分化
公开(公告)号：WO2004081205A8
公开(公告)日：2004-10-28
申请号：PCT/AU2004000302
申请日：2004-03-11
申请人： ES CELL INT PTE LTD , NETHERLANDS INST VOOR ONTWIKKE , IP ORGANISERS PTY LTD , MUMMERY CHRISTINE LINDSAY , DOEVENDANS PETRUS ADRIANUS FRE , TERTOOLEN LEON GERARDUS JOSEPH
发明人： MUMMERY CHRISTINE LINDSAY , DOEVENDANS PETRUS ADRIANUS FRE , TERTOOLEN LEON GERARDUS JOSEPH
IPC分类号： C12N5/077 , C12N5/08 , C12N5/10
CPC分类号： C12N5/0657 , C12N2502/02 , C12N2506/02
摘要： A method for inducing cardiomyocyte differentiation of a hES cell, the method comprising co-culturing the hES cell with a cell excreting at least one cardiomyocyte differentiation inducing factor or with an extracellular medium therefrom, under conditions that induce differentiation, cells and cell populations so produced, and uses of the cells.
摘要翻译：诱导hES细胞的心肌细胞分化的方法，所述方法包括在诱导分化的条件下，将hES细胞与分泌至少一种心肌细胞分化诱导因子的细胞或其细胞外培养基共培养，如此产生的细胞和细胞群，以及细胞的使用。

4. 发明申请

WO2005118784A1 IMPROVED CARDIOMYOCYTE DIFFERENTIATION 审中-公开
标题翻译：改善心肌细胞分化
公开(公告)号：WO2005118784A1
公开(公告)日：2005-12-15
申请号：PCT/AU2004/000727
申请日：2004-06-01
申请人： ES CELL INTERNATIONAL PTE LTD , IP ORGANISERS PTY LTD , MUMMERY, Christine Lindsay , PASSIER, Robert
发明人： MUMMERY, Christine Lindsay , PASSIER, Robert
IPC分类号： C12N5/08
CPC分类号： C12N5/0657 , C12N5/0606 , C12N2500/25 , C12N2500/90 , C12N2500/99 , C12N2502/02 , C12N2503/02 , C12N2506/02 , C12Q1/68 , G01N33/5008 , G01N33/5026 , G01N33/5073 , G01N33/53
摘要： The present invention provides a method of enhancing the efficiency of differentiation of hES cells into cardiomyocytes which method comprises incubating the cells under serem free conditions. The method typically includes providing cells that induce cardiomyocyte differentiation by cell to cell contact. Differentiation to cardiomyocytes can occur via two routes, namely by spontaneous differentiation and by induced differentiation. Without wishing to be bound by theory, the present inventors hypthesise that, in the case of induced differentiation, END-2 cells, for instance, are needed for aggregation to cause local high cell densities and in inducing differentation of nascent mesodern. This second step could be enhanced in any human embryonic stem cell line leading to the prediction that it will work in lines other than hES. In cell lines that undergo sponantaneous differentiation, it is hypothesised that local induction of embryoid bodies in edoderm occurs. Typically for induced differentiation this method will also comprise culturing the hES cell with a cell excreting at least one cardiomyocyte differentiation inducing factor or with an extracellular medium therefrom, under conditions that induce differentiation.
摘要翻译：本发明提供了一种提高hES细胞分化为心肌细胞的效率的方法，该方法包括在无细胞条件下孵育细胞。该方法通常包括提供通过细胞与细胞接触诱导心肌细胞分化的细胞。通过两种途径发生心肌细胞分化，即通过自发分化和诱导分化。本发明人不希望受理论的束缚，因此，诱导分化时，例如需要使用END-2细胞进行聚集以引起局部高细胞密度并引起新生中胚层的分化。在任何人类胚胎干细胞系中可以增强第二步，从而预测它将在除了hES之外的其它线条中起作用。在经历自发分化的细胞系中，假设发生了edoderm中胚状体的局部诱导。通常，为了诱导分化，该方法还将包括在诱导分化的条件下，用至少一种心肌细胞分化诱导因子或其细胞外培养基的细胞培养hES细胞。

5. 发明申请

WO2006066320A1 DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS AND CARDIOMYOCYTES AND CARDIOMYOCYTE PROGENITORS DERIVED THEREFROM 审中-公开
标题翻译：人类胚胎干细胞和细胞周质蛋白的分化及其衍生的心脏病原体
公开(公告)号：WO2006066320A1
公开(公告)日：2006-06-29
申请号：PCT/AU2005/001921
申请日：2005-12-21
申请人： ES CELL INTERNATIONAL PTE LTD , IP ORGANISERS PTY LTD , PASSIER, Robert , MUMMERY, Christine, Lindsay
发明人： PASSIER, Robert , MUMMERY, Christine, Lindsay
IPC分类号： C12N5/08
CPC分类号： C12N5/0657 , A61K35/12 , C12N2500/38 , C12N2500/90 , C12N2506/02
摘要： The present invention provides a method to improve current culturing methods for the differentiation of cardiomyocytes from hES cells. The method includes culturing the hES cells in the presence of ascorbic acid or a derivative thereof. Preferably the culturing is conducted in serum free conditions. The invention also includes isolated cardiomyocytes and cardiac progenitors differentiated by the methods as well as the use of these cells in methods of treating and preventing cardiac diseases and conditions. Culture media and extracellular media are also provided which include ascorbic acid for the differentiation of hES cells to cardiomyocytes.
摘要翻译：本发明提供了一种改善目前用于从hES细胞分化心肌细胞的培养方法的方法。该方法包括在抗坏血酸或其衍生物存在下培养hES细胞。优选培养在无血清条件下进行。本发明还包括通过方法分化的分离的心肌细胞和心脏祖细胞以及这些细胞在治疗和预防心脏疾病和病症的方法中的用途。还提供了培养基和细胞外培养基，其包括用于将hES细胞分化为心肌细胞的抗坏血酸。

6. 发明申请

WO2003010303A1 METHODS OF INDUCING DIFFERENTIATION OF STEM CELLS 审中-公开
标题翻译：诱导干细胞分化的方法
公开(公告)号：WO2003010303A1
公开(公告)日：2003-02-06
申请号：PCT/AU2002/000978
申请日：2002-07-23
申请人： ES CELL INTERNATIONAL PTE LTD , NETHERLANDS INSTITUUT VOOR ONTWIKKELINGSBIOLOGIE , MUMMERY, Christine, Lindsay
发明人： MUMMERY, Christine, Lindsay
IPC分类号： C12N5/08
CPC分类号： C12N5/0606 , A61K35/12 , C12N5/0603 , C12N5/0657 , C12N5/0658 , C12N5/069 , C12N2501/10 , C12N2501/155 , C12N2502/02 , C12N2502/1329 , C12N2502/28 , C12N2506/02 , C12N2510/00
摘要： The present invention relates to methods of inducing differentiation of stem cells. In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. The invention also includes cells, cell lines, testing models and culture systems used in the methods of the present invention and differentiated cells produced therefrom. The present invention also provides methods of using the differentiated cells of the present invention for therapeutic purposes.
摘要翻译：本发明涉及诱导干细胞分化的方法。特别地，本发明涉及诱导胚胎干细胞分化成肌肉细胞或血管内皮细胞的方法。本发明还包括在本发明的方法中使用的细胞，细胞系，测试模型和培养系统以及由其产生的分化细胞。本发明还提供了将本发明的分化细胞用于治疗目的的方法。

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