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1. 发明申请

WO2013040572A2 COMPOSITIONS AND METHODS FOR THE PRODUCTION AND USE OF HUMAN CHOLINESTERASES 审中-公开
标题翻译：用于生产和使用人胆碱酯酶的组合物和方法
公开(公告)号：WO2013040572A2
公开(公告)日：2013-03-21
申请号：PCT/US2012055781
申请日：2012-09-17
申请人： UNIV ARIZONA , MOR TSAFRIR , KANNAN LATHA , LARRIMORE KATHERINE E
发明人： MOR TSAFRIR , KANNAN LATHA , LARRIMORE KATHERINE E
IPC分类号： C12N15/83 , C12N9/16 , C12N15/29 , C12N15/55
CPC分类号： C12N9/18 , C12N15/8255 , C12N15/8257 , C12Y301/01008
摘要： In some aspects, the present invention relates to compositions and methods for the production of human cholinesterases. More particularly, it relates to methods for the production of human cholinesterases using transient expression and vectors for producing the same. In one aspect, the present invention relates to a plant viral vector encoding a plant codon-optimized DNA sequence that results in accumulation of the cholinesterase in a plant leaf at levels greater than 20 mg, and in some embodiments greater than 200 mg, of the enzyme per kilogram of the plant leaf.
摘要翻译：在一些方面，本发明涉及用于生产人胆碱酯酶的组合物和方法。更具体地说，涉及使用瞬时表达产生人类胆碱酯酶的方法以及用于生产人类胆碱酯酶的载体。在一个方面，本发明涉及编码植物密码子优化的DNA序列的植物病毒载体，其导致胆碱酯酶在植物叶中以大于20mg，并且在一些实施方案中大于200mg的水平酶每千克植物叶子。

2. 发明申请

WO2003075849A2 COMPOSITION AND METHOD FOR ENHANCING IMMUNE RESPONSE 审中-公开
标题翻译：用于增强免疫应答的组合物和方法
公开(公告)号：WO2003075849A2
公开(公告)日：2003-09-18
申请号：PCT/US2003/007073
申请日：2003-03-06
申请人： ARIZONA BOARD OF REGENTS , MOR, Tsafrir, S. , MATOBA, Nobuyuki , ARNTZEN, Charles, J.
发明人： MOR, Tsafrir, S. , MATOBA, Nobuyuki , ARNTZEN, Charles, J.
IPC分类号： A61K
CPC分类号： C07K14/005 , A61K39/39 , A61K2039/541 , A61K2039/55516 , A61K2039/6037 , A61K2039/6075 , C12N2740/16122
摘要： A composition and method for enhancing immune response in a living organism is disclosed. In particular, the present disclosure provides an adjuvant peptide for use in raising an immune response to an antigen. The adjuvant peptide is selected from a group of peptides with an HIV-related sequence. Additionally, the adjuvant peptide can comprise a fusion-protein that acts as a mucosal adjuvant. The adjuvant peptide can be transformed into one or more living cells, such that the mucosal adjuvant can be produced in living cells and then administered by systemic, mucosal or epidermal delivery.
摘要翻译：公开了一种增强活体内免疫应答的组合物和方法。特别地，本公开提供了用于提高对抗原的免疫应答的佐剂肽。佐剂肽选自具有HIV相关序列的肽组。另外，佐剂肽可以包含充当粘膜佐剂的融合蛋白。辅助肽可以转化成一个或多个活细胞，使得粘膜佐剂可以在活细胞中产生，然后通过全身，粘膜或表皮递送进行施用。

3. 发明申请

WO2013040572A8 COMPOSITIONS AND METHODS FOR THE PRODUCTION AND USE OF HUMAN CHOLINESTERASES 审中-公开
公开(公告)号：WO2013040572A8
公开(公告)日：2013-03-21
申请号：PCT/US2012/055781
申请日：2012-09-17
申请人： ARIZONA BOARD OF REGENTS FOR AND ON BEHALF OF ARIZONA STATE UNIVERSITY , MOR, Tsafrir , KANNAN, Latha , LARRIMORE, Katherine, E.
发明人： MOR, Tsafrir , KANNAN, Latha , LARRIMORE, Katherine, E.
IPC分类号： C12N15/83 , C12N15/29 , C12N15/55 , C12N9/16
摘要： In some aspects, the present invention relates to compositions and methods for the production of human cholinesterases. More particularly, it relates to methods for the production of human cholinesterases using transient expression and vectors for producing the same. In one aspect, the present invention relates to a plant viral vector encoding a plant codon-optimized DNA sequence that results in accumulation of the cholinesterase in a plant leaf at levels greater than 20 mg, and in some embodiments greater than 200 mg, of the enzyme per kilogram of the plant leaf.

4. 发明申请

WO0171014A3 EXPRESSION OF RECOMBINANT HUMAN ACETYLCHOLINESTERASE IN TRANSGENIC PLANTS 审中-公开
标题翻译：重组人乙酰胆碱酯酶在转基因植物中的表达
公开(公告)号：WO0171014A3
公开(公告)日：2002-05-30
申请号：PCT/US0108468
申请日：2001-03-16
申请人： THOMPSON BOYCE PLANT RES , MOR TSAFRIR , SOREQ HERMONA , ARNTZEN CHARLES , MASON HUGH
发明人： MOR TSAFRIR , SOREQ HERMONA , ARNTZEN CHARLES , MASON HUGH
IPC分类号： C12N9/18 , C12N15/12 , C12N15/82 , A01H1/00 , A01H5/10 , A61K38/46 , C12N5/10
CPC分类号： C12N15/8242 , C12N9/18 , C12N15/8257
摘要： Briefly stated, the invention includes a method of making a transgenic plant that is capable of expressing a physiologically active human acetylcholinesterase, comprising the steps of introducing into at least one plant cell a polynucleotide that encodes a human acetylcholinesterase, and regenerating from the plant cell a transgenic plant that is capable of expressing a physiologically active human acetylcholinesterase in at least one tissue type of the transgenic plant. Another embodiment of the invention includes a method of making a physiologically active human acetylcholinesterase, comprising the steps of introducing into at least one plant cell a polynucleotide that encodes a human acetylcholinesterase, regenerating from the plant cell a transgenic plant that is capable of expressing a physiologically active human acetylcholinesterase in at least one tissue type of the transgenic plant, and isolating or purifying from the transgenic plant or a part thereof a physiologically active human acetylcholinesterase.
摘要翻译：简言之，本发明包括制备能够表达生理活性的人乙酰胆碱酯酶的转基因植物的方法，其包括以下步骤：向至少一个植物细胞中引入编码人乙酰胆碱酯酶的多核苷酸，并从植物细胞再生能够在至少一种组织类型的转基因植物中表达生理活性的人乙酰胆碱酯酶的转基因植物。本发明的另一个实施方案包括制备生理活性的人乙酰胆碱酯酶的方法，该方法包括以下步骤：将从编码人乙酰胆碱酯酶的多核苷酸引入至少一个植物细胞，从植物细胞再生能够表达生理学的转基因植物在至少一种组织类型的转基因植物中活性的人乙酰胆碱酯酶，并从转基因植物或其部分分离或纯化生理活性的人乙酰胆碱酯酶。

5. 发明申请

WO2015050627A1 PLANT-BASED RECOMBINANT BUTYRYLCHOLINESTERASE PRODUCTION METHODS 审中-公开
标题翻译：基于植物的重组丁基胆碱酯酶生产方法
公开(公告)号：WO2015050627A1
公开(公告)日：2015-04-09
申请号：PCT/US2014/049387
申请日：2014-08-01
申请人： POGUE, Greg , HIATT, Ernie , KANDZIA, Romy , WERNER, Stefan , THIEME, Frank , MOR, Tsafrir
发明人： POGUE, Greg , HIATT, Ernie , KANDZIA, Romy , WERNER, Stefan , THIEME, Frank , MOR, Tsafrir
IPC分类号： C12N9/00
CPC分类号： C12N9/18 , A61K38/465 , C12Y301/01008
摘要： A new, reliable, easily scalable and reproducible method for the production of recombinant butyrylcholinesterase (rBuChE) is provided. Through the utilization of a plant transfection procedure, various plant strains have been shown to generate effective and scalable amounts of rBuChE under acceptable manufacturing processes to permit reliable levels of such enzymes for desired nerve agent protection requirements (including tetrameric products). As well, such methods in engineered plant lines have shown suitable production of these enzymes in tetramer form with glycan formation and sialyalation (for terminal groups) to allow for optimal potency against organophosphorus agent exposure as well as proper immunogenic response within the plant sources. The overall production method, including the transfection and production within mammalian cells, as well as the process steps involved for such a reliable sourcing platform from plants is thus encompassed within the invention.
摘要翻译：提供了一种用于生产重组丁酰胆碱酯酶（rBuChE）的新的，可靠的，易于扩展和重现的方法。通过利用植物转染方法，已经显示各种植物菌株在可接受的制造过程中产生有效和可缩放量的rBuChE，以允许可靠水平的这些酶用于所需的神经药物保护要求（包括四聚物产品）。同样，工程化植物品系中的这些方法已经显示出以四聚体形式合适地生产这些酶，其中聚糖形成和sialyalation（用于末端基团）以允许针对有机磷剂暴露的最佳效力以及在植物来源内的适当的免疫原性反应。因此，包括哺乳动物细胞内的转染和生产的总体生产方法以及从植物获得这种可靠的采购平台所涉及的工艺步骤。

6. 发明申请

WO2007040568A3 PRODUCTION AND USE OF HUMAN BUTYRYLCHOLINESTERASE 审中-公开
公开(公告)号：WO2007040568A3
公开(公告)日：2007-04-12
申请号：PCT/US2005/043929
申请日：2005-12-01
申请人： ARIZONA BOARD OF REGENTS, acting for and on behalf of ARIZONA STATE UNIVERISTY , MOR, Tsafrir, S. , GEYER, Brian, C.
发明人： MOR, Tsafrir, S. , GEYER, Brian, C.
IPC分类号： C12N15/82 , A01H5/00 , C07H21/04
摘要： The present invention concerns the production of human butyrylcholinesterase (BuChE) in transgenic plants and use of the derived BuChE as effective countermeasures against toxic agents such as pesticides, toxins, certain drugs and non-conventional warfare agents, as well as treatments for diseases and conditions associated with depressed cholinesterase levels.

7. 发明申请

WO2013040572A3 COMPOSITIONS AND METHODS FOR THE PRODUCTION AND USE OF HUMAN CHOLINESTERASES 审中-公开
标题翻译：用于生产和使用人类胆固醇的组合物和方法
公开(公告)号：WO2013040572A3
公开(公告)日：2013-06-13
申请号：PCT/US2012055781
申请日：2012-09-17
申请人： UNIV ARIZONA , MOR TSAFRIR , KANNAN LATHA , LARRIMORE KATHERINE E
发明人： MOR TSAFRIR , KANNAN LATHA , LARRIMORE KATHERINE E
IPC分类号： C12N15/83 , C12N9/16 , C12N15/29 , C12N15/55
CPC分类号： C12N9/18 , C12N15/8255 , C12N15/8257 , C12Y301/01008
摘要： In some aspects, the present invention relates to compositions and methods for the production of human cholinesterases. More particularly, it relates to methods for the production of human cholinesterases using transient expression and vectors for producing the same. In one aspect, the present invention relates to a plant viral vector encoding a plant codon-optimized DNA sequence that results in accumulation of the cholinesterase in a plant leaf at levels greater than 20 mg, and in some embodiments greater than 200 mg, of the enzyme per kilogram of the plant leaf.
摘要翻译：在一些方面，本发明涉及用于生产人胆碱酯酶的组合物和方法。更具体地说，本发明涉及使用瞬时表达生产人胆碱酯酶的方法和用于生产人胆碱酯酶的方法。一方面，本发明涉及编码植物密码子优化的DNA序列的植物病毒载体，其导致胆碱酯酶在植物叶中的积累，其水平大于20mg，在一些实施方案中大于200mg，酶每千克植物叶。

8. 发明申请

WO2009024957A1 MATERIALS AND METHODS FOR TREATING OR PREVENTING ORGANOPHOSPHATE EXPOSURE ASSOCIATED DAMAGE 审中-公开
标题翻译：用于治疗或预防有机磷酸盐暴露相关损害的材料和方法
公开(公告)号：WO2009024957A1
公开(公告)日：2009-02-26
申请号：PCT/IL2007/001032
申请日：2007-08-20
申请人： YISSUM RESEARCH DEVELOPMENT COMPANY OF THE HEBREW UNIVERSITY OF JERUSALEM , SOREQ, Hermona , MOR, Tsafrir
发明人： SOREQ, Hermona , MOR, Tsafrir
IPC分类号： A61K38/46 , A61K9/00 , A61P39/00
CPC分类号： A61K9/0019 , A61K9/0073 , A61K9/7007 , A61K38/465 , C12Y301/01007
摘要： A method of treating or preventing organophosphate exposure associated damage in a subject in need thereof is provided. The method comprising providing the subject with a therapeutically effective amount of the acetylcholinesterase splice variant AChE-R ("readthrough" AChE) to thereby. treat the organophosphate exposure associated damage in the subject.
摘要翻译：提供了在有需要的受试者中治疗或预防有机磷酸盐暴露相关损伤的方法。该方法包括向受试者提供治疗有效量的乙酰胆碱酯酶剪接变体AChE-R（“通过”AChE）“。治疗受试者中有机磷酸盐暴露相关的损伤。

9. 发明申请

WO2007040568A2 PRODUCTION AND USE OF HUMAN BUTYRYLCHOLINESTERASE 审中-公开
标题翻译：人丁酰胆碱酯酶的生产和使用
公开(公告)号：WO2007040568A2
公开(公告)日：2007-04-12
申请号：PCT/US2005043929
申请日：2005-12-01
申请人： UNIV ARIZONA , MOR TSAFRIR S , GEYER BRIAN C
发明人： MOR TSAFRIR S , GEYER BRIAN C
IPC分类号： A01H5/00 , C12N5/04
CPC分类号： C12N15/8257
摘要： The present invention concerns the production of human butyrylcholinesterase (BuChE) in transgenic plants and use of the derived BuChE as effective countermeasures against toxic agents such as pesticides, toxins, certain drugs and non-conventional warfare agents, as well as treatments for diseases and conditions associated with depressed cholinesterase levels.
摘要翻译：本发明涉及在转基因植物中生产人丁酰胆碱酯酶（BuChE），以及使用衍生的BuChE作为有效对抗有毒物质如杀虫剂，毒素，某些药物和非常规战剂以及治疗疾病和病症的有效对策与胆碱酯酶水平降低有关。

10. 发明申请

WO2003075849A3 COMPOSITION AND METHOD FOR ENHANCING IMMUNE RESPONSE 审中-公开
公开(公告)号：WO2003075849A3
公开(公告)日：2003-09-18
申请号：PCT/US2003/007073
申请日：2003-03-06
申请人： ARIZONA BOARD OF REGENTS , MOR, Tsafrir, S. , MATOBA, Nobuyuki , ARNTZEN, Charles, J.
发明人： MOR, Tsafrir, S. , MATOBA, Nobuyuki , ARNTZEN, Charles, J.
IPC分类号： A61K39/12
摘要： A composition and method for enhancing immune response in a living organism is disclosed. In particular, the present disclosure provides an adjuvant peptide for use in raising an immune response to an antigen. The adjuvant peptide is selected from a group of peptides with an HIV-related sequence. Additionally, the adjuvant peptide can comprise a fusion-protein that acts as a mucosal adjuvant. The adjuvant peptide can be transformed into one or more living cells, such that the mucosal adjuvant can be produced in living cells and then administered by systemic, mucosal or epidermal delivery.

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