专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2015142679A1 PRODUCTION OF HIGHLY THERMALLY STABLE RECOMBINANT CHOLINESTERASES FOR THE DETECTION, DETOXIFICATION AND DECONTAMINATION OF ORGANOPHOSPHORUS COMPOUNDS 审中-公开
标题翻译：生产用于检测，去氧化和去除有机磷化合物的高度稳定的重组胆碱酯酶
公开(公告)号：WO2015142679A1
公开(公告)日：2015-09-24
申请号：PCT/US2015/020623
申请日：2015-03-14
申请人： ROSENBERG, Yvonne
发明人： ROSENBERG, Yvonne
IPC分类号： C07K7/08 , A61K38/10
CPC分类号： C12N9/18 , A61K38/00 , A61K38/465 , A61K47/36 , A62D3/02 , A62D2101/02 , A62D2101/26 , C07K7/08 , C07K2319/04 , C07K2319/21 , C07K2319/43 , C12Q1/46 , C12Y301/01008
摘要： Disclosed herein are methods for the large-scale production of a highly thermally stable acetylcholinesterase (AChE) and butyrylcholmesterase (BChE). Additionally, the expression methods disclosed herein can produce ChE preparations consisting of extract or purified forms that can be produced in high amounts and are highly thermally stable. These ChE products can be used in vitro detection, detoxification and decontamination methods.
摘要翻译：本文公开了大规模生产高度热稳定的乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BChE）的方法。此外，本文公开的表达方法可以产生由可以高产量并且是高度热稳定的提取物或纯化形式组成的ChE制剂。这些ChE产品可用于体外检测，解毒和去污方法。

2. 发明申请

WO2015050627A1 PLANT-BASED RECOMBINANT BUTYRYLCHOLINESTERASE PRODUCTION METHODS 审中-公开
标题翻译：基于植物的重组丁基胆碱酯酶生产方法
公开(公告)号：WO2015050627A1
公开(公告)日：2015-04-09
申请号：PCT/US2014/049387
申请日：2014-08-01
申请人： POGUE, Greg , HIATT, Ernie , KANDZIA, Romy , WERNER, Stefan , THIEME, Frank , MOR, Tsafrir
发明人： POGUE, Greg , HIATT, Ernie , KANDZIA, Romy , WERNER, Stefan , THIEME, Frank , MOR, Tsafrir
IPC分类号： C12N9/00
CPC分类号： C12N9/18 , A61K38/465 , C12Y301/01008
摘要： A new, reliable, easily scalable and reproducible method for the production of recombinant butyrylcholinesterase (rBuChE) is provided. Through the utilization of a plant transfection procedure, various plant strains have been shown to generate effective and scalable amounts of rBuChE under acceptable manufacturing processes to permit reliable levels of such enzymes for desired nerve agent protection requirements (including tetrameric products). As well, such methods in engineered plant lines have shown suitable production of these enzymes in tetramer form with glycan formation and sialyalation (for terminal groups) to allow for optimal potency against organophosphorus agent exposure as well as proper immunogenic response within the plant sources. The overall production method, including the transfection and production within mammalian cells, as well as the process steps involved for such a reliable sourcing platform from plants is thus encompassed within the invention.
摘要翻译：提供了一种用于生产重组丁酰胆碱酯酶（rBuChE）的新的，可靠的，易于扩展和重现的方法。通过利用植物转染方法，已经显示各种植物菌株在可接受的制造过程中产生有效和可缩放量的rBuChE，以允许可靠水平的这些酶用于所需的神经药物保护要求（包括四聚物产品）。同样，工程化植物品系中的这些方法已经显示出以四聚体形式合适地生产这些酶，其中聚糖形成和sialyalation（用于末端基团）以允许针对有机磷剂暴露的最佳效力以及在植物来源内的适当的免疫原性反应。因此，包括哺乳动物细胞内的转染和生产的总体生产方法以及从植物获得这种可靠的采购平台所涉及的工艺步骤。

3. 发明申请

WO2014011901A2 METHODS AND COMPOSITIONS FOR DELIVERY OF BIOLOGICS 审中-公开
标题翻译：递送生物制品的方法和组合物
公开(公告)号：WO2014011901A2
公开(公告)日：2014-01-16
申请号：PCT/US2013/050107
申请日：2013-07-11
申请人： SANGAMO BIOSCIENCES, INC.
发明人： COST, Gregory J.
IPC分类号： A61K35/18
CPC分类号： C12N15/907 , A61K35/18 , A61K38/465 , C12Y301/01008
摘要： Nucleases and methods of using these nucleases for genetic alteration of red blood cells (RBCs), for example for providing for a protein lacking in a monogenic disorder or a biologic for the treatment of exposure to a toxin using genetically altered RBCs.
摘要翻译：使用这些核酸酶进行红细胞（RBC）基因改变的核酸酶和方法，例如用于提供缺乏单基因病症的蛋白质或用于使用毒素暴露治疗的生物制剂遗传改变的红细胞。

4. 发明申请

WO2007001302A1 DELIVERY OF ACTIVE PROTEINS TO THE CENTRAL NERVOUS SYSTEM USING PHAGE VECTORS 审中-公开
标题翻译：使用噬菌体载体将活性蛋白递送到中央神经系统
公开(公告)号：WO2007001302A1
公开(公告)日：2007-01-04
申请号：PCT/US2005/022955
申请日：2005-06-28
申请人： THE SCRIPPS RESEARCH INSTITUTE , JANDA, Kim, J.
发明人： JANDA, Kim, J.
IPC分类号： A01N43/04 , A61K31/70 , A61K39/00 , A61K39/38 , C12N15/00 , C12N15/09 , C12N15/63 , C12N15/70 , C12N15/74 , C12N5/00 , C12N5/02
CPC分类号： C12N15/86 , A61K38/465 , C07K2319/735 , C12N15/1037 , C12N2795/14143 , C12Y301/01008
摘要： A method of delivering a protein to the central nervous system in active form comprises: (1) preparing a single-stranded filamentous bacteriophage vector comprising a nucleic acid construct in which a protein to be delivered to the central nervous system is encoded as a fusion protein with a coat protein of a filamentous phage; (2) preparing phage particles incorporating the nucleic acid construct as the phage genome and in which the fusion protein is expressed as a coat protein; and (3) delivering the phage particles to a mammal by a route such that the phage particles reach the central nervous system so that the protein is delivered to the central nervous system in active form. The protein to be delivered can be an antibody, an enzyme, a reporter protein, a receptor, or another type of protein. The method has wide diagnostic and therapeutic application. The invention also encompasses nucleic acid constructs, bacteriophage particles including the protein to be delivered, and pharmaceutical compositions.
摘要翻译：将蛋白质以活性形式递送至中枢神经系统的方法包括：（1）制备包含核酸构建体的单链丝状噬菌体载体，其中待递送至中枢神经系统的蛋白质被编码为融合蛋白具有丝状噬菌体的外壳蛋白; （2）制备掺入核酸构建体的噬菌体颗粒作为噬菌体基因组，其中融合蛋白被表达为外壳蛋白; 和（3）通过使噬菌体颗粒到达中枢神经系统的途径将噬菌体颗粒递送到哺乳动物，使得蛋白质以活性形式递送至中枢神经系统。待递送的蛋白质可以是抗体，酶，报道蛋白，受体或另一种类型的蛋白质。该方法具有广泛的诊断和治疗应用。本发明还包括核酸构建体，包括待递送蛋白质的噬菌体颗粒和药物组合物。

5. 发明申请

WO2004092340A3 BUTYRYLCHOLINESTERASE VARIANT POLYPEPTIDES WITH INCREASED CATALYTIC EFFICIENCY AND METHODS OF USE 审中-公开
标题翻译：具有增加的催化效率的丁二烯基酯改性聚异戊二烯和使用方法
公开(公告)号：WO2004092340A3
公开(公告)日：2005-07-07
申请号：PCT/US2004011225
申请日：2004-04-09
申请人： APPLIED MOLECULAR EVOLUTION , WATKINS JEFFRY D , PANCOOK JAMES D
发明人： WATKINS JEFFRY D , PANCOOK JAMES D
IPC分类号： A61K38/00 , A61K38/46 , A61K48/00 , C07H21/04 , C12N9/16 , C12N9/18 , C12Q1/68
CPC分类号： C12N9/18 , A61K38/00 , C12Y301/01008
摘要： The invention provides a butyrylcholinesterase variant having increased cocaine hydrolysis activity as well as the corresponding encoding nucleic acid. The invention further provides methods of hydrolyzing a cocaine-based butyrylcholinesterase substrate as well as methods of treating a cocaine-induced condition with the invention variant.
摘要翻译：本发明提供了具有增加的可卡因水解活性的丁酰胆碱酯酶变体以及相应的编码核酸。本发明还提供了水解可卡因类丁酰胆碱酯酶底物的方法以及用本发明变体治疗可卡因诱导病症的方法。

6. 发明申请

WO02064796A8 BUTYRYLCHOLINESTERASE POLYPEPTIDE VARIANTS WITH INCREASED CATALYTIC EFFICIENCY AND METHODS OF USE 审中-公开
标题翻译：提高催化效率的丁酰胆碱酯酶多肽变体及使用方法
公开(公告)号：WO02064796A8
公开(公告)日：2003-11-13
申请号：PCT/US0150450
申请日：2001-12-21
申请人： APPLIED MOLECULAR EVOLUTION , UNIV NEBRASKA MEDICAL CT , LOCKRIDGE OKSANA , WATKINS JEFFRY D , PANCOOK JAMES D
发明人： LOCKRIDGE OKSANA , WATKINS JEFFRY D , PANCOOK JAMES D
IPC分类号： C12N15/09 , A61K38/00 , A61K38/46 , A61P25/30 , A61P39/02 , C12N9/18 , C12N15/55 , C12Q1/46
CPC分类号： C12Q1/46 , A61K38/00 , C12N9/18 , C12Y301/01008
摘要： The invention provides twenty-five butyrylcholinesterase variants having increased cocaine hydrolysis activity as well as the corresponding encoding nucleic acids. The invention also provides libraries of butyrylcholinesterase variants as well as libraries of the corresponding nucleic acids encoding butyrylcholinesterase variants. The invention further provides methods of hydrolyzing a cocaine-based butyrylcholinesterase substrate as well as methods of treating a cocaine-induced condition.
摘要翻译：本发明提供了具有增加的可卡因水解活性的二十五丁酰胆碱酯酶变体以及相应的编码核酸。本发明还提供丁酰胆碱酯酶变体的文库以及编码丁酰胆碱酯酶变体的相应核酸的文库。本发明进一步提供水解可卡因基丁酰胆碱酯酶底物的方法以及治疗可卡因诱导病症的方法。

7. 发明申请

WO2015168284A1 ALBUMIN-BCHE IN DETOXIFICATION OF NEUROTOXINS 审中-公开
标题翻译： ALBUMIN-BCHE在脱氧神经毒素中的应用
公开(公告)号：WO2015168284A1
公开(公告)日：2015-11-05
申请号：PCT/US2015/028291
申请日：2015-04-29
申请人： TEVA PHARMACEUTICAL INDUSTRIES LTD. , BASSAN, Merav , GROSS, Aviva , SINGH, Mallika , SEAVEY, Matthew, M. , BOCK, Jason , ELIAZ, Rom, E.
发明人： BASSAN, Merav , GROSS, Aviva , SINGH, Mallika , SEAVEY, Matthew, M. , BOCK, Jason , ELIAZ, Rom, E.
IPC分类号： C12N9/18
CPC分类号： C12N9/18 , A61K31/15 , A61K38/465 , C07K2319/31 , C12Y301/01008 , A61K2300/00
摘要： The present invention provides the recombinant fusion protein of SEQ ID No :1. and a method of treating a mammal for exposure to a neurotoxin or for preventing or ameliorating the Neurotoxic Symptoms of exposure to neurotoxin which comprises administering an effective amount of the recombinant fusion protein of SEQ ID No :1. SEQ ID No:1 is a fully recombinant fusion polypeptide composed of the mature form of human serum albumin (HSA) fused at its amino terminus to the carboxy- terminus of a truncated and mutated human BChE.
摘要翻译：本发明提供了SEQ ID No：1的重组融合蛋白。以及治疗哺乳动物暴露于神经毒素或用于预防或改善暴露于神经毒素的神经毒性症状的方法，其包括施用有效量的SEQ ID No：1的重组融合蛋白。 SEQ ID No：1是由其血清白蛋白（HSA）的成熟形式组成的完全重组融合多肽，其在其氨基末端与截短和突变的人类BChE的羧基末端融合。

8. 发明申请

WO2015077317A1 COMPOSITIONS AND METHODS FOR THE PURIFICATION AND PRODUCTION OF BUTYRYLCHOLINESTERASE 审中-公开
标题翻译：用于纯化和生产丁基胆碱酯酶的组合物和方法
公开(公告)号：WO2015077317A1
公开(公告)日：2015-05-28
申请号：PCT/US2014/066373
申请日：2014-11-19
申请人： BOARD OF REGENTS OF THE UNIVERSITY OF NEBRASKA , UNIVERSITE DE ROUEN , SERVICE DE SANTE DES ARMEES , UNIVERSITE NICE SOPHIA ANTIPOLIS , RENARD, Pierre-yves , LOCKRIDGE, Oksana , LARSON, Marilynn, A. , SCHOPFER, Lawrence, M. , HINRICHS, Steven, H. , NACHON, Florian , BRAZZOLOTTO, Xavier , RONCO, Cyril , JEAN, Ludovic , DAVID, Emilie
发明人： LOCKRIDGE, Oksana , LARSON, Marilynn, A. , SCHOPFER, Lawrence, M. , HINRICHS, Steven, H. , NACHON, Florian , BRAZZOLOTTO, Xavier , RONCO, Cyril , JEAN, Ludovic , DAVID, Emilie
IPC分类号： A61K38/46 , C12N15/09 , C12N15/55 , C12N9/18
CPC分类号： C12N9/18 , A61K38/465 , C12Y301/01008
摘要： Methods and compositions for producing BChE tetramers and purifying BChE disclosed.
摘要翻译： BChE四聚体的生产方法和组合物和BChE的纯化。

9. 发明申请

WO2015057822A1 CATALYTIC SCAVENGERS OF ORGANOPHOSPHATES TO POTENTIATE BUTYRYLCHOLINESTERASE (BCHE) AS A CATALYTIC BIOSCAVENGER AND METHODS FOR MAKING AND USING THEM 审中-公开
标题翻译：有机磷酸酯的催化消毒剂，作为催化剂生产者及其制备和使用方法，将丁基胆碱酯酶（BCHE）
公开(公告)号：WO2015057822A1
公开(公告)日：2015-04-23
申请号：PCT/US2014/060666
申请日：2014-10-15
申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA , THE SCRIPPS RESEARCH INSTITUTE
发明人： TAYLOR, Palmer , RADIC, Zoran , FOKIN, Valery
IPC分类号： A61K31/15 , A61K31/415 , A01N33/24
CPC分类号： A61K31/4164 , A61K31/4439 , A61K31/5377 , A61K38/465 , A61K45/06 , C12Y301/01008
摘要： Provided are N-alkyl imidazole 2-aldoximes, including cationic imidazolium and uncharged tertiary imidazole aldoximes, and compositions and methods for making and using them, including methods for reactivating human butyrylcholinesterase (hBChE) or acetylcholinesterase (hAChE ) inhibited by organophosphate (OP). By administration of a composition of the invention, the inactive or conjugated hBChE-OP or hAChE-OP is reactivated and the catalytic cycle of turnover and inactivation of the OP is completed; and in alternative embodiments, secondary mechanisms of reversible protection of hBChE and hAChE from irreversible inactivation by OPs and reactivation of tissue AChE also contribute to overall efficacy.
摘要翻译：提供的是N-烷基咪唑2-肟，包括阳离子咪唑和不带电的叔咪唑肟，以及其制备和使用它们的组合物和方法，包括用有机磷酸酯（OP）抑制的人丁酰胆碱酯酶（hBChE）或乙酰胆碱酯酶（hAChE）的再活化方法。通过施用本发明的组合物，使活化或缀合的hBChE-OP或hAChE-OP重新活化，完成OP的转换和灭活的催化循环; 并且在替代实施方案中，可逆保护hBChE和hAChE免受OPs的不可逆失活和组织AChE再活化的次要机制也有助于整体疗效。

10. 发明申请

WO2014182684A2 METHODS AND COMPOSITIONS RELATED TO LARGE SCALE PRODUCTION OF PROTEINS 审中-公开
标题翻译：与大规模生产蛋白质相关的方法和组合物
公开(公告)号：WO2014182684A2
公开(公告)日：2014-11-13
申请号：PCT/US2014/036947
申请日：2014-05-06
申请人： CELL MACHINES, INC.
发明人： KELLY, James
IPC分类号： A61K38/37
CPC分类号： C07K14/76 , A61K38/00 , C07K14/755 , C07K16/00 , C07K16/468 , C07K2317/31 , C07K2317/622 , C07K2317/94 , C07K2319/31 , C12N9/18 , C12N9/644 , C12Y301/01008 , C12Y304/21022
摘要： Disclosed herein are methods and compositions related to synthetic fusion proteins and engineered cells.
摘要翻译：本文公开了与合成融合蛋白和工程细胞相关的方法和组合物。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式