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1. 发明申请

WO2014190419A1 FREE-FLOW ELECTROPHORESIS DEVICE AND METHOD 审中-公开
标题翻译：自由流动电泳装置及方法
公开(公告)号：WO2014190419A1
公开(公告)日：2014-12-04
申请号：PCT/CA2014/000468
申请日：2014-05-30
申请人： AGOSTINO, Fletcher John , KRYLOV, Sergey N.
发明人： AGOSTINO, Fletcher John , KRYLOV, Sergey N.
IPC分类号： G01N27/453
CPC分类号： G01N27/44769 , B01D17/06 , B01D19/0068 , B01L3/502715 , B01L3/502723 , B01L2200/0684 , B01L2300/089 , B01L2400/0421
摘要： There is provided an electrophoresis device. The device includes a chamber, for containing liquid medium, the liquid medium including a flowing separation medium, carrying a sample, a pair of opposing electrodes for generating an electric field, with effect that the generated electric field effects spatial separation of the sample into sample fractions and, with respect to at least one of the electrodes, also effects generation of gaseous material from the liquid medium disposed at, or substantially at, an operative electrode surface of the electrode, with effect that the generated gaseous material becomes, at least initially, disposed within the liquid medium, wherein the generated gaseous material includes at least one generated gaseous compound, and at least one outlet for collecting a sample fraction. In some embodiments, for example, the device further includes a gas separator for inducing removal of at least a fraction of the generated gaseous material from the chamber, the gas separator including a space configured for effecting fluid communication between the liquid medium and a generated gaseous material receiving fluid phase such that a fluid interface is defined between the liquid medium and the generated gaseous material receiving fluid phase, and such that at least a fraction of the generated gaseous material migrates upwardly from its disposition at, or substantially at, the operative electrode surface to the fluid interface in response to buoyancy forces, and then from the fluid interface and into the generated gaseous material receiving fluid phase in response to a driving force. In some embodiments, the device further includes flow guides, disposed between the electrodes, for directing the flowing separation medium towards the at least one outlet. In some implementations, the flow guides mitigate the creation of a pH gradient.
摘要翻译：提供了电泳装置。该装置包括用于容纳液体介质的液体介质，液体介质包括流动的分离介质，携带样品，一对相对的电极，用于产生电场，其作用是产生的电场影响样品到样品的空间分离并且相对于至少一个电极还可以从设置在电极的有效电极表面处或基本上位于电极的有效电极表面处的液体介质产生气体材料，从而产生的气态材料至少起初设置在所述液体介质内，其中所产生的气态材料包括至少一种产生的气态化合物，以及用于收集样品级分的至少一个出口。在一些实施例中，例如，该装置还包括气体分离器，用于从室中诱导至少一部分产生的气态材料的移除，气体分离器包括被配置用于在液体介质和产生的气体之间实现流体连通的空间材料接收流体相，使得在液体介质和产生的接收流体相的气态材料之间限定流体界面，并且使得至少一部分所产生的气态材料从其在或在其上的操作电极的位置向上迁移响应于浮力而将表面传递到流体界面，然后响应于驱动力从流体界面流入生成的气态物质接收流体相。在一些实施例中，该装置还包括设置在电极之间的流动引导件，用于将流动的分离介质引向至少一个出口。在一些实施方式中，流动导向器减轻了pH梯度的产生。

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