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1. 发明申请

WO2012139110A3 PEPTIDE CONSTRUCTS AND ASSAY SYSTEMS 审中-公开
标题翻译：肽结构和测定系统
公开(公告)号：WO2012139110A3
公开(公告)日：2012-12-27
申请号：PCT/US2012032759
申请日：2012-04-09
申请人： PROGNOSYS BIOSCIENCES INC , KOZLOV IGOR A , CHEE MARK S , CAPEK PETR , ROUTENBERG DAVID A
发明人： KOZLOV IGOR A , CHEE MARK S , CAPEK PETR , ROUTENBERG DAVID A
IPC分类号： C40B40/08 , C40B40/10 , C40B50/06
CPC分类号： G01N33/6803 , C12N15/1062 , C12N15/1075 , C12Q2521/537
摘要： The disclosure provides methods for constructing peptide construct sets and methods of use of these peptide construct sets in assay systems for peptide analysis, and in particular for use in high throughput peptide analysis. The methods allow for analysis of large sets of peptide constructs in a cost-effective manner, employing molecular biological techniques that are both robust and easily parallelized. Thus, the methods allow for the construction of peptide construct sets encompassing, e.g., the human proteome.
摘要翻译：本公开提供了用于构建肽构建体组的方法以及用于肽分析的测定系统中这些肽构建体组的使用方法，特别是用于高通量肽分析。该方法允许以成本有效的方式分析大量肽构建体，使用分子生物学技术，其稳健且容易并行化。因此，所述方法允许构建包括例如人类蛋白质组的肽构建体组。

2. 发明申请

WO2011127006A1 CO-LOCALIZATION AFFINITY ASSAYS 审中-公开
标题翻译：共定位亲和力测定
公开(公告)号：WO2011127006A1
公开(公告)日：2011-10-13
申请号：PCT/US2011/031163
申请日：2011-04-05
申请人： PROGNOSYS BIOSCIENCES, INC. , CHEE, Mark, S. , KOZLOV, Igor, A. , WENTWORTH, Anita, D. , KOSAKOVSKY POND, Stephanie, J.
发明人： CHEE, Mark, S. , KOZLOV, Igor, A. , WENTWORTH, Anita, D. , KOSAKOVSKY POND, Stephanie, J.
IPC分类号： C12Q1/68
CPC分类号： G01N33/6845 , G01N2458/10
摘要： The invention provides a new assay format for high throughput molecular binding studies at a single molecule level. The invention enables creation of binding event identifiers in a highly parallel way. Individual binding events occur between two agents of a binding pair, e.g. , a protein-based binding pair or a binding pair comprising a protein and a chemical moiety. The binding event identifier created through the binding of the two binding agents is unique to that pair, and identification of the binding event identifier is indicative of the binding of these specific may be assessed through a readout that is digital in nature. The invention enables very large sets of thousands or more of different binding agents or potential binding agents to be assayed simultaneously, resolving millions or more of potential interactions, and distinguishing specific interactions from those that are less specific.
摘要翻译：本发明提供了在单一分子水平上用于高通量分子结合研究的新的测定形式。本发明能够以高度并行的方式创建绑定事件标识符。个体结合事件发生在结合对的两个试剂之间，例如基于蛋白质的结合对或包含蛋白质和化学部分的结合对。通过两个绑定代理的绑定而创建的绑定事件标识符对于该对是唯一的，并且绑定事件标识符的标识指示可以通过本质上是数字的读出来评估这些特定的绑定。本发明使得能够同时测定成千上万或更多种不同结合剂或潜在的结合剂的非常大的组，解决数百万或更多的潜在相互作用，并且将特异性相互作用与不太具体的相互作用区分开来。

3. 发明申请

WO2011014879A3 ASSAY TOOLS AND METHODS OF USE 审中-公开
标题翻译：测定工具和使用方法
公开(公告)号：WO2011014879A3
公开(公告)日：2011-05-05
申请号：PCT/US2010044134
申请日：2010-08-02
申请人： PROGNOSYS BIOSCIENCES INC , CHEE MARK S , KOZLOV IGOR A
发明人： CHEE MARK S , KOZLOV IGOR A
IPC分类号： G01N33/573 , C12Q1/34 , C12Q1/37 , G01N33/543
CPC分类号： G01N33/54366 , C12Q1/34 , C12Q1/37 , G01N33/573
摘要： The present invention provides assay tools for the detection of biological or chemical activity in a sample. The assay tools of the invention provide direct detection using a positive signal generated on a surface of the assay tool. These assay tools provide improved methods for detection and/or identification of multiple agents (e.g., enzymes) in a sample, analysis of substrate specificity of such agents, and binding affinities and specificities of such agents. Upon activity a component is released from a first immobilised construct and then captured by a capture surface. At least two different immobilised constructs are used.
摘要翻译：本发明提供了用于检测样品中生物或化学活性的测定工具。本发明的测定工具使用在测定工具的表面上产生的阳性信号提供直接检测。这些测定工具提供了用于样品中多个试剂（例如酶）的检测和/或鉴定的改进方法，这些试剂的底物特异性的分析以及这些试剂的结合亲和力和特异性。在活动时，将组分从第一固定化构建物释放，然后由捕获表面捕获。使用至少两种不同的固定化构建体。

4. 发明申请

WO2011071943A1 PEPTIDE DISPLAY ARRAYS 审中-公开
标题翻译：肽显示阵列
公开(公告)号：WO2011071943A1
公开(公告)日：2011-06-16
申请号：PCT/US2010/059327
申请日：2010-12-07
申请人： PROGNOSYS BIOSCIENCES, INC. , CHEE, Mark, S. , KOZLOV, Igor, A.
发明人： CHEE, Mark, S. , KOZLOV, Igor, A.
IPC分类号： C12Q1/68 , G01N33/50
CPC分类号： G01N33/54366 , B01J2219/005 , B01J2219/00533 , B01J2219/00596 , B01J2219/00612 , B01J2219/00626 , B01J2219/00659 , B01J2219/00722 , B01J2219/00725 , B01J2219/0074 , C07K2319/43 , C12N15/1062 , C12N15/1075 , C40B50/18 , C40B80/00 , C12Q2525/191 , C12Q2563/131 , C12Q2563/149 , C12Q2565/501
摘要： The present invention provides arrays, methods of constructing arrays, and methods of use of such arrays. The arrays of the invention comprise a substrate with two or more discrete constructs or discrete sets of constructs associated on the surface of the substrate, optionally via a linker molecule. The constructs include an oligonucleotide comprising a region encoding a peptide of interest and an affinity tag and an untranslated region, a fusion peptide comprising both the peptide of interest and the affinity tag and a capture agent that forms a binding pair with the affinity tag.
摘要翻译：本发明提供阵列，构造阵列的方法以及这种阵列的使用方法。本发明的阵列包括具有两个或更多个离散构建体或与衬底表面相关联的离散构建体的衬底，任选经由接头分子。构建体包括寡核苷酸，其包含编码目的肽的区域和亲和标签和非翻译区，融合肽包含目标肽和亲和标签，以及与亲和标签形成结合对的捕获剂。

5. 发明申请

WO2007067923A3 COMPOSITIONS AND METHODS FOR DETECTING PHOSPHOMONOESTER 审中-公开
标题翻译：用于检测磷酸化合物的组合物和方法
公开(公告)号：WO2007067923A3
公开(公告)日：2007-11-22
申请号：PCT/US2006061681
申请日：2006-12-06
申请人： ILLUMINA INC , KOZLOV IGOR , MELNYK PETER , ZHAO CHANFENG
发明人： KOZLOV IGOR , MELNYK PETER , ZHAO CHANFENG
IPC分类号： G01N33/53 , C07K14/47
CPC分类号： G01N33/5308 , C07K1/1072 , C07K1/13
摘要： The invention provides a method of modifying a phosphomonoester moiety of a target compound. The method can include the steps of (a) providing a target compound having an electrophilic moiety and a phosphomonoester moiety; (b) contacting the target compound with a first carbodiimide compound under conditions for preferential addition of the first carbodiimide compound to the electrophilic moiety over the phosphomonoester moiety, thereby forming an electrophile-protected target compound; and (c) contacting the electrophile-protected target compound with a second carbodiimide compound and a nucleophilic compound under conditions for addition of the nucleophilic compound to the phosphomonoester.
摘要翻译：本发明提供了修饰目标化合物的磷酸单酯部分的方法。该方法可以包括以下步骤：（a）提供具有亲电子部分和磷酸单酯部分的目标化合物; （b）使所述目标化合物与第一碳化二亚胺化合物在优先向所述磷酸单酯部分上的所述亲电子部分加入所述第一碳二亚胺化合物的条件下接触，由此形成亲电子受保护的目标化合物; 和（c）在亲核化合物加入磷酸单酯的条件下，使亲电试剂保护的目标化合物与第二碳化二亚胺化合物和亲核化合物接触。

6. 发明申请

WO2007067923A2 COMPOSITIONS AND METHODS FOR DETECTING PHOSPHOMONOESTER 审中-公开
标题翻译：用于检测磷酸酯的组合物和方法
公开(公告)号：WO2007067923A2
公开(公告)日：2007-06-14
申请号：PCT/US2006/061681
申请日：2006-12-06
申请人： ILLUMINA, INC. , KOZLOV, Igor , MELNYK, Peter , ZHAO, Chanfeng
发明人： KOZLOV, Igor , MELNYK, Peter , ZHAO, Chanfeng
IPC分类号： G01N33/53 , C07K14/47
CPC分类号： G01N33/5308 , C07K1/1072 , C07K1/13
摘要： The invention provides a method of modifying a phosphomonoester moiety of a target compound. The method can include the steps of (a) providing a target compound having an electrophilic moiety and a phosphomonoester moiety; (b) contacting the target compound with a first carbodiimide compound under conditions for preferential addition of the first carbodiimide compound to the electrophilic moiety over the phosphomonoester moiety, thereby forming an electrophile-protected target compound; and (c) contacting the electrophile-protected target compound with a second carbodiimide compound and a nucleophilic compound under conditions for addition of the nucleophilic compound to the phosphomonoester.
摘要翻译：本发明提供了修饰目标化合物的磷酸单酯部分的方法。该方法可以包括以下步骤：（a）提供具有亲电子部分和磷酸酯部分的目标化合物; （b）在优先将第一碳二亚胺化合物添加到磷酸单酯部分上的亲电子部分的条件下，使目标化合物与第一碳二亚胺化合物接触，由此形成亲电试剂保护的目标化合物; 和（c）在向磷酸单酯添加亲核性化合物的条件下，将亲电试剂保护的目标化合物与第二碳二亚胺化合物和亲核化合物接触。

7. 发明申请

WO2005059180A1 METHODS OF ATTACHING OLIGONUCLEOTIDES AND PEPTIDES TO SOLID SUPPORTS USING 2,4-HYDRAZIN- (1, 3, 5) TRIAZINE-ACTIVATED SOLID PHASES 审中-公开
标题翻译：使用2,4-HYDRAZIN-（1,3,5）TRIAZINE激活的固体相将连接寡核苷酸和肽固定在固体支持物上的方法
公开(公告)号：WO2005059180A1
公开(公告)日：2005-06-30
申请号：PCT/US2004/041885
申请日：2004-12-10
申请人： ILLUMINA, INC. , KOZLOV, Igor , ZHAO, Chanfeng , STEINBERG-TATMAN, Galina
发明人： KOZLOV, Igor , ZHAO, Chanfeng , STEINBERG-TATMAN, Galina
IPC分类号： C12Q1/68
CPC分类号： C07D251/12 , C07H21/00 , C07H21/04 , Y10T428/139
摘要： A compound of Formula I: (I) wherein X 1 and X 2 are each independently selected from the group consisting of chloro, NH-NH 2 , NH-N=CH-E-T-L-D, wherein E is a bond or an electron withdrawing group; T is selected from a bond, -C(O)-, -C(O)NH-, -NHC(O)-, an oxygen atom, a sulfur atom, NH, -S(O)-, -SO 2 -, or alkyl substituted with one or more substituents selected from the group consisting of dialkylamine, NO 2 , CN, SO 3 H, COOH, CHO, alkoxy, and halogen; L is a linker selected from the group consisting of a bond, -(CH 2 ) p -, -(CH 2 ) p -O-, (CH 2 -CH 2 -O) p -, and -(CH 2 ) p -S(O) 2 -; and D is a biologically active polymer; Q is a solid surface; V is selected from a bond, NH, CO, NHCO, C(O)NH, (CH 2 ) p , sulfur, and oxygen, or a combination thereof; and p is an integer greater than or equal to zero; as well as methods of attaching oligonucleotides and peptides to solid supports using 2,4-hydrazin-(1, 3, 5) triazine-activated solid phases.
摘要翻译：式I的化合物：其中X 1和X 2各自独立地选自氯，NH-NH 2，NH-N = CH-ETLD，其中E是键或电子退出组 T选自-C（O） - ， - C（O）NH - ， - NHC（O） - ，氧原子，硫原子，NH，-S（O） - ，-SO 2 - 或被一个或多个选自二烷基胺，NO 2，CN，SO 3 H，COOH，CHO，烷氧基和卤素的取代基取代的烷基; L是选自键， - （CH 2）p - ，（CH 2）p -O-，（CH 2 -CH 2 -O）p - 和 - （CH 2）p -S（O）2 - 的取代基; D是生物活性聚合物; Q是固体表面; V选自键，NH，CO，NHCO，C（O）NH，（CH 2）p，硫和氧，或其组合; 并且p是大于或等于零的整数; 以及使用2,4-肼基 - （1,3,5）三嗪活化的固相将寡核苷酸和肽连接到固体支持物的方法。

8. 发明申请

WO2008042067A3 COMPOSITIONS AND METHODS FOR NUCLEOTIDE SEQUENCING 审中-公开
标题翻译：核苷酸序列的组合物和方法
公开(公告)号：WO2008042067A3
公开(公告)日：2008-10-02
申请号：PCT/US2007019353
申请日：2007-09-04
申请人： ILLUMINA INC , ZHAO CHANFENG , KOZLOV IGOR , SHULTS MELISSA
发明人： ZHAO CHANFENG , KOZLOV IGOR , SHULTS MELISSA
IPC分类号： C07H19/04 , C12Q1/68
CPC分类号： C12Q1/6869 , C07H19/04 , C12P19/34 , C12Q1/6823 , C12Q2525/117 , C12Q2525/186 , C12Q2563/107
摘要： The invention provides nucleoside and nucleotide molecules containing cleavable linkers linking a label such as a dye. The invention also provides nucleosides and nucleotide molecules containing a blocking group, either removable or non-removable. The invention additionally provides methods of using the nucleoside and nucleotide molecules containing a cleavable linker and/or a blocking group.
摘要翻译：本发明提供了含有连接标记如染料的可切割连接子的核苷和核苷酸分子。本发明还提供含有可移除或不可除去的封闭基团的核苷和核苷酸分子。本发明另外提供了使用含有可裂解连接子和/或封闭基团的核苷和核苷酸分子的方法。

9. 发明申请

WO2006104778A3 COMPOSITIONS AND METHODS FOR DETECTING PROTEASE ACTIVITY 审中-公开
公开(公告)号：WO2006104778A3
公开(公告)日：2006-10-05
申请号：PCT/US2006/010277
申请日：2006-03-21
申请人： ILLUMINA , INC. et al. , KOZLOV, Igor , MELNYK, Peter
发明人： KOZLOV, Igor , MELNYK, Peter
IPC分类号： C07B61/00 , C07H21/02 , C07H21/04 , C07K2/00 , C12N15/10 , C12Q1/68 , A61K38/00
摘要： The invention provides a method of determining activity of a protease. The method can include the steps of (a) providing a protease substrate including a protein moiety attached to a nucleic acid moiety and a ligand moiety; (b) contacting the protease substrate with a protease under conditions wherein the protease catalyzes cleavage of the protein moiety, thereby producing a proteolytic product wherein the nucleic acid moiety is separated from at least a portion of the protein moiety and the ligand moiety; (c) contacting the proteolytic product with a receptor under conditions wherein the ligand moiety binds to the receptor to form a complex; (d) separating the complex from the nucleic acid moiety, thereby forming a separation product including the nucleic acid moiety; (e) contacting the separation product with a probe nucleic acid under conditions wherein the nucleic acid moiety hybridizes to a complementary sequence of the probe; and (f) detecting hybridization of the separation product to the probe, thereby determining activity of the protease.

10. 发明申请

WO1991018109A1 TEMPERATURE CHANGE INDICATOR EMPLOYING ENZYMES 审中-公开
标题翻译：温度变化指示剂使用酶
公开(公告)号：WO1991018109A1
公开(公告)日：1991-11-28
申请号：PCT/GB1991000815
申请日：1991-05-23
申请人： RAYBOURNE LIMITED , KOZLOV, Igor, Anatolievich , PARTON, Adrian
发明人： RAYBOURNE LIMITED
IPC分类号： C12Q01/00
CPC分类号： G01N31/229 , C12Q1/00 , C12Q1/26
摘要： Enzyme reactions may be carried out in a non-aqueous medium comprising a C12-C20 aliphatic alcohol such as lauryl alcohol. The medium may be a mixture of such an alcohol with a C6-C30 aliphatic hydrocarbon such as hexane or decane. The principle may be used to produce a temperature change indicator, comprising a solid non-aqueous medium comprising a C12-C20 aliphatic alcohol such as lauryl alcohol and a C6-C30 aliphatic hydrocarbon such as hexane or decane, together with an enzyme, a substrate for the enzyme, and a development reagent capable of producing a detectable change, on reaction of the substrate with the enzyme. The medium is chosen to melt at a specific temperature, or in a specific temperature range, to permit reaction to occur between the enzyme, the substrate, and the development reagent. Preferably, the enzyme is mushroom phenol oxidase, the enzyme substrate is o-dihydroxyphenol, and the development reagent is an ester of an amino acid with a C4-C20 alcohol, for example the butyl ester of proline.
摘要翻译：酶反应可以在包含C12-C20脂族醇如月桂醇的非水介质中进行。介质可以是这样的醇与C 6 -C 30脂族烃如己烷或癸烷的混合物。该原理可用于制备温度变化指示剂，其包含固体非水介质，其包含C 12 -C 20脂族醇如月桂醇和C 6 -C 30脂族烃如己烷或癸烷，以及酶，底物以及在底物与酶反应时能够产生可检测变化的显影试剂。选择该介质在特定温度或特定温度范围内熔化，以允许在酶，底物和显影试剂之间发生反应。优选地，酶是蘑菇酚氧化酶，酶底物是邻二羟基苯酚，显色剂是氨基酸与C 4 -C 20醇的酯，例如脯氨酸的丁酯。

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