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1. 发明申请

WO2016191646A3 AUTOMATED METHOD AND SYSTEM FOR OBTAINING AND PREPARING MICROORGANISM SAMPLE FOR BOTH IDENTIFICATION AND ANTIBIOTIC SUSCEPTIBILITY TESTS 审中-公开
标题翻译：用于获取和制备微生物样品的自动方法和系统，用于两种鉴定和抗生素的不可测试性
公开(公告)号：WO2016191646A3
公开(公告)日：2017-01-26
申请号：PCT/US2016034554
申请日：2016-05-27
申请人： BD KIESTRA B V , HANSEN TIMOTHY R , HOLTZ RICK , KLEEFSTRA MARTIJN , MARCELPOIL RAPHAEL RODOLPHE , PIERPONT RICK , POHL BRENT RONALD , SHEDLOSKY ALYSSA , SHINDLEDECKER SCOTT , SKEVINGTON EDWARD , SMITH KERRY LYNN , WILES TIMOTHY
发明人： HANSEN TIMOTHY R , HOLTZ RICK , KLEEFSTRA MARTIJN , MARCELPOIL RAPHAEL RODOLPHE , PIERPONT RICK , POHL BRENT RONALD , SHEDLOSKY ALYSSA , SHINDLEDECKER SCOTT , SKEVINGTON EDWARD , SMITH KERRY LYNN , WILES TIMOTHY
IPC分类号： G01N1/38 , G01N35/00 , G01N35/10
CPC分类号： G01N35/00029 , C12M33/04 , G01N1/38 , G01N35/0099 , G01N35/10 , G01N2035/00039 , G01N2035/00306 , G01N2035/1032
摘要： A method and automated apparatus for locating and selecting a colony of microorganisms on a culture dish and subjecting the obtained sample to a plurality of downstream tests including a test to identify the microorganism and a test to identify the susceptibility of the microorganism to antibiotics. The method includes the automated steps of locating and selecting a colony of microorganisms on a culture dish; obtaining a sample of the selected colony of microorganisms; preparing a suspension of a sample of microorganisms automatically by submerging the pick tool with the sample in a suspension, after which the pick tool is vibrated in at least the vertical direction to release the sample from the pick tool in the suspension. The turbidity of the suspension is monitored to ensure that the concentration of microorganism in suspension is sufficient so that the suspension is used a source for sample for both identification and antibiotic susceptibility of the microorganisms in the sample. The apparatus and system optionally provides for downstream processing of samples prepared for antibiotic susceptibility testing (AST). Such apparatus includes further processing after inoculation of an AST panel for the AST test. Such further processing includes capping and transferring inoculated panels to AST instrument.
摘要翻译：一种用于在培养皿上定位和选择微生物菌落的方法和自动化装置，并使得到的样品进行多次下游测试，包括鉴定微生物的试验和用于鉴定微生物对抗生素敏感性的试验。该方法包括在培养皿上定位和选择微生物菌落的自动化步骤; 获得所选择的微生物菌落的样品; 通过将采样工具与样品浸没在悬浮液中来自动制备微生物样品的悬浮液，然后拾取工具至少在垂直方向上振动，以将样品从悬浮液中的拾取工具中释放出来。监测悬浮液的浊度，以确保悬浮液中的微生物浓度足以使悬浮液用于样品来源，用于样品中微生物的鉴定和抗生素敏感性。该装置和系统任选提供用于抗生素敏感性试验（AST）制备的样品的下游处理。这样的装置包括在用于AST测试的AST面板接种之后的进一步处理。这种进一步的处理包括将接种的面板封盖并转移到AST仪器。

2. 发明申请

WO2012148273A1 DISC DISPENSING DEVICE, TUBULAR CONTAINER FOR USE IN SUCH A DISC DISPENSING DEVICE AND METHOD OF DISPENSING DISCS 审中-公开
标题翻译： DISC分配装置，用于这种碟盘分配装置的管状容器和分配盘的方法
公开(公告)号：WO2012148273A1
公开(公告)日：2012-11-01
申请号：PCT/NL2012/050288
申请日：2012-04-27
申请人： KIESTRA LAB AUTOMATION DRACHTEN B.V. , BOTMA, Jetze , KLEEFSTRA, Martijn , VAN DER KAAP, Trienko Marten , BERNTSEN, Martijn Xander , VAN DER VIJVER, Jan Bart
发明人： BOTMA, Jetze , KLEEFSTRA, Martijn , VAN DER KAAP, Trienko Marten , BERNTSEN, Martijn Xander , VAN DER VIJVER, Jan Bart
IPC分类号： C12M1/00 , G01N35/00 , G01N35/10
CPC分类号： G01N35/1002 , C12M41/48 , C12M99/00 , C12M99/02
摘要： Disc dispensing device for dispensing discs impregnated with antibiotics onto a culture dish. The device comprises a stationary support on which a magazine is removably mounted. The magazine holds a plurality of tubular containers each containing a stack of discs to be dispensed. A moving means moves one disc from a bottom end of a tubular container to a pick-up 10 position. From the pick-up position a transfer means picks up said one disc and transfers it to the culture dish where it is dispensed. The removable culture dish is supported by a moveable carriage which is mounted on the stationary support. The carriage is moveable relative to the magazine from a starting position to an operating position and vice versa. Control means control the 15 operation of the device. The plurality of tubular containers is rotatable around a magazine centre under control of the control means.
摘要翻译：用于将浸渍有抗生素的盘分配到培养皿上的盘分配装置。该装置包括一个固定支架，一个仓可移动地安装在该固定支架上。杂志保持多个管状容器，每个管状容器包含要分配的一叠盘。移动装置将一个盘从管状容器的底端移动到拾取器10的位置。从拾取位置，转印装置拾取所述一个盘并将其转移到其分配的培养皿中。可移动培养皿由安装在固定支架上的可移动托架支撑。托架可相对于料仓从起始位置移动到操作位置，反之亦然。控制装置控制设备的15个操作。多个管状容器在控制装置的控制下可围绕盒子中心旋转。

3. 发明申请

WO2016191646A2 AUTOMATED METHOD AND SYSTEM FOR OBTAINING AND PREPARING MICROORGANISM SAMPLE FOR BOTH IDENTIFICATION AND ANTIBIOTIC SUSCEPTIBILITY TESTS 审中-公开
标题翻译：用于获取和制备微生物样品的自动方法和系统，用于两种鉴定和抗生素的不可测试性
公开(公告)号：WO2016191646A2
公开(公告)日：2016-12-01
申请号：PCT/US2016/034554
申请日：2016-05-27
申请人： BD KIESTRA B.V. , HANSEN, Timothy, R. , HOLTZ, Rick , KLEEFSTRA, Martijn , MARCELPOIL, Raphael, Rodolphe , PIERPONT, Rick , POHL, Brent, Ronald , SHEDLOSKY, Alyssa , SHINDLEDECKER, Scott , SKEVINGTON, Edward , SMITH, Kerry, Lynn , WILES, Timothy
发明人： HANSEN, Timothy, R. , HOLTZ, Rick , KLEEFSTRA, Martijn , MARCELPOIL, Raphael, Rodolphe , PIERPONT, Rick , POHL, Brent, Ronald , SHEDLOSKY, Alyssa , SHINDLEDECKER, Scott , SKEVINGTON, Edward , SMITH, Kerry, Lynn , WILES, Timothy
IPC分类号： G01N1/38 , G01N35/00 , G01N35/10
CPC分类号： G01N35/00029 , C12M33/04 , G01N1/38 , G01N35/0099 , G01N35/10 , G01N2035/00039 , G01N2035/00306 , G01N2035/1032
摘要： A method and automated apparatus for locating and selecting a colony of microorganisms on a culture dish and subjecting the obtained sample to a plurality of downstream tests including a test to identify the microorganism and a test to identify the susceptibility of the microorganism to antibiotics. The method includes the automated steps of locating and selecting a colony of microorganisms on a culture dish; obtaining a sample of the selected colony of microorganisms; preparing a suspension of a sample of microorganisms automatically by submerging the pick tool with the sample in a suspension, after which the pick tool is vibrated in at least the vertical direction to release the sample from the pick tool in the suspension. The turbidity of the suspension is monitored to ensure that the concentration of microorganism in suspension is sufficient so that the suspension is used a source for sample for both identification and antibiotic susceptibility of the microorganisms in the sample. The apparatus and system optionally provides for downstream processing of samples prepared for antibiotic susceptibility testing (AST). Such apparatus includes further processing after inoculation of an AST panel for the AST test. Such further processing includes capping and transferring inoculated panels to AST instrument.
摘要翻译：一种用于在培养皿上定位和选择微生物菌落的方法和自动化装置，并使得到的样品进行多次下游测试，包括鉴定微生物的试验和用于鉴定微生物对抗生素敏感性的试验。该方法包括在培养皿上定位和选择微生物菌落的自动化步骤; 获得所选择的微生物菌落的样品; 通过将采样工具与样品浸没在悬浮液中来自动制备微生物样品的悬浮液，然后拾取工具至少在垂直方向上振动，以将样品从悬浮液中的拾取工具中释放出来。监测悬浮液的浊度，以确保悬浮液中的微生物浓度足以使悬浮液用于样品来源，用于样品中微生物的鉴定和抗生素敏感性。该装置和系统任选提供用于抗生素敏感性试验（AST）制备的样品的下游处理。这样的装置包括在用于AST测试的AST面板接种之后的进一步处理。这种进一步的处理包括将接种的面板封盖并转移到AST仪器。

4. 发明申请

WO2013147610A3 AUTOMATED SELECTION OF MICROORGANISMS AND IDENTIFICATION USING MALDI 审中-公开
标题翻译：使用MALDI自动选择微生物和鉴定
公开(公告)号：WO2013147610A3
公开(公告)日：2014-01-09
申请号：PCT/NL2013050239
申请日：2013-04-02
申请人： BOTMA JETZE , KLEEFSTRA MARTIJN , VAN DER ZEE TINO WALTER
发明人： BOTMA JETZE , KLEEFSTRA MARTIJN , VAN DER ZEE TINO WALTER
IPC分类号： G01N35/10 , C12M1/22 , C12M1/26 , C12M1/36 , G01N1/04 , G01N35/00 , H01J49/16
CPC分类号： G01N35/00029 , C12M23/10 , C12M33/04 , C12M41/48 , C12Q3/00 , G01N35/10 , G01N2001/045 , G01N2035/1032 , H01J49/164
摘要： A method and apparatus for locating and selecting a colony of microorganisms on a culture dish and identifying microorganisms in said selected colony using MALDI. The method comprises the automated steps of locating and selecting a colony of microorganisms on a culture dish; obtaining a sample of said selected colony of microorganisms; depositing at least some of said sample of said selected colony of microorganisms on a target plate; and transferring said target plate with said sample in an apparatus for performing MALDI for identification of said sample of said selected colony of microorganisms. A sample of a colony of microorganisms is automatically deposited on a depositing spot such that the sample covers at most approximately half of said one of the depositing spots of the target plate. A suspension of a sample of microorganisms is automatically prepared by automatically picking the sample with a picking tool and submerging the picking tool with said sample in a suspension, after which the picking tool is vibrated in vertical sense only to release the sample from the picking tool.
摘要翻译：一种用于在培养皿上定位和选择微生物菌落并使用MALDI鉴定所述选定菌落中的微生物的方法和装置。该方法包括在培养皿上定位和选择微生物菌落的自动化步骤; 获得所述选定的微生物菌落的样品; 将所述选定的微生物菌落的所述样品中的至少一些沉积在靶板上; 以及在用于进行MALDI鉴定所述所选微生物菌落样品的设备中用所述样品转移所述靶板。微生物菌落的样品自动沉积在沉积点上，使得样品至少覆盖目标板的所述一个沉积点的大约一半。微生物样品的悬浮液通过用拾取工具自动采样样品并将采样工具用所述样品浸没在悬浮液中而自动制备，之后拾取工具在垂直方向上振动，以将样品从采摘工具中释放出来。

5. 发明申请

WO2013147610A2 AUTOMATED SELECTION OF MICROORGANISMS AND IDENTIFICATION USING MALDI 审中-公开
标题翻译：使用MALDI自动选择微生物和鉴定
公开(公告)号：WO2013147610A2
公开(公告)日：2013-10-03
申请号：PCT/NL2013/050239
申请日：2013-04-02
申请人： BOTMA, Jetze , KLEEFSTRA, Martijn , VAN DER ZEE, Tino Walter
发明人： BOTMA, Jetze , KLEEFSTRA, Martijn , VAN DER ZEE, Tino Walter
IPC分类号： G01N35/10
CPC分类号： G01N35/00029 , C12M23/10 , C12M33/04 , C12M41/48 , C12Q3/00 , G01N35/10 , G01N2001/045 , G01N2035/1032 , H01J49/164
摘要： A method and apparatus for locating and selecting a colony of microorganisms on a culture dish and identifying microorganisms in said selected colony using MALDI. The method comprises the automated steps of locating and selecting a colony of microorganisms on a culture dish; obtaining a sample of said selected colony of microorganisms; depositing at least some of said sample of said selected colony of microorganisms on a target plate; and transferring said target plate with said sample in an apparatus for performing MALDI for identification of said sample of said selected colony of microorganisms. A sample of a colony of microorganisms is automatically deposited on a depositing spot such that the sample covers at most approximately half of said one of the depositing spots of the target plate. A suspension of a sample of microorganisms is automatically prepared by automatically picking the sample with a picking tool and submerging the picking tool with said sample in a suspension, after which the picking tool is vibrated in vertical sense only to release the sample from the picking tool.
摘要翻译：一种用于在培养皿上定位和选择微生物菌落并使用MALDI鉴定所述选定菌落中的微生物的方法和装置。该方法包括在培养皿上定位和选择微生物菌落的自动化步骤; 获得所述选定的微生物菌落的样品; 将所述选定的微生物菌落的所述样品中的至少一些沉积在靶板上; 以及在用于进行MALDI鉴定所述所选微生物菌落样品的设备中用所述样品转移所述靶板。微生物菌落的样品自动沉积在沉积点上，使得样品至少覆盖目标板的所述一个沉积点的大约一半。微生物样品的悬浮液通过用拾取工具自动采样样品并将采样工具用所述样品浸没在悬浮液中而自动制备，之后拾取工具在垂直方向上振动，以将样品从采摘工具中释放出来。

6. 发明申请

WO2009061183A1 METHODS AND APPARATUS FOR HANDLING MICROBIAL SAMPLES 审中-公开
标题翻译：用于处理微生物样品的方法和装置
公开(公告)号：WO2009061183A1
公开(公告)日：2009-05-14
申请号：PCT/NL2008/050693
申请日：2008-11-04
申请人： KIESTRA LAB-AUTOMATION B.V. , VAN DER KAAP, Trienko Marten , KLEEFSTRA, Martijn , TJERNBERG, Ingela Marie Sofie Edlund , BOTMA, Jetze
发明人： VAN DER KAAP, Trienko Marten , KLEEFSTRA, Martijn , TJERNBERG, Ingela Marie Sofie Edlund , BOTMA, Jetze
IPC分类号： C12M1/32
CPC分类号： C12Q1/24 , C12M33/02 , Y10T428/2982
摘要： This invention pertains to the general field of microbiology, and more specifically to transfer, inoculation and/or streaking of micro-organisms, e.g. for the purpose of obtaining individual colonies. Provided is a method for streaking a microbial sample onto a solid carrier, comprising the steps of: a) contacting at least one ferromagnetic particle with a solid carrier, followed or preceded by providing the particle with at least part of said sample, and b) applying a magnetic field gradient to allow for magnetically controlled motion of said particle on said surface, such that at least part of the sample is streaked onto the solid carrier. Also provided is an apparatus for carrying out such method in an (semi-)automated fashion.
摘要翻译：本发明涉及微生物学的一般领域，更具体地涉及微生物的转移，接种和/或条纹，例如微生物。为了获得个体殖民地的目的。提供了一种将微生物样品划到固体载体上的方法，包括以下步骤：a）使至少一个铁磁颗粒与固体载体接触，之后或之前为所述颗粒提供至少部分所述样品，以及b）施加磁场梯度以允许所述颗粒在所述表面上的磁控制运动，使得至少部分样品划线到固体载体上。还提供了一种以（半）自动方式执行这种方法的装置。

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