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1. 发明申请

WO2016191646A3 AUTOMATED METHOD AND SYSTEM FOR OBTAINING AND PREPARING MICROORGANISM SAMPLE FOR BOTH IDENTIFICATION AND ANTIBIOTIC SUSCEPTIBILITY TESTS 审中-公开
标题翻译：用于获取和制备微生物样品的自动方法和系统，用于两种鉴定和抗生素的不可测试性
公开(公告)号：WO2016191646A3
公开(公告)日：2017-01-26
申请号：PCT/US2016034554
申请日：2016-05-27
申请人： BD KIESTRA B V , HANSEN TIMOTHY R , HOLTZ RICK , KLEEFSTRA MARTIJN , MARCELPOIL RAPHAEL RODOLPHE , PIERPONT RICK , POHL BRENT RONALD , SHEDLOSKY ALYSSA , SHINDLEDECKER SCOTT , SKEVINGTON EDWARD , SMITH KERRY LYNN , WILES TIMOTHY
发明人： HANSEN TIMOTHY R , HOLTZ RICK , KLEEFSTRA MARTIJN , MARCELPOIL RAPHAEL RODOLPHE , PIERPONT RICK , POHL BRENT RONALD , SHEDLOSKY ALYSSA , SHINDLEDECKER SCOTT , SKEVINGTON EDWARD , SMITH KERRY LYNN , WILES TIMOTHY
IPC分类号： G01N1/38 , G01N35/00 , G01N35/10
CPC分类号： G01N35/00029 , C12M33/04 , G01N1/38 , G01N35/0099 , G01N35/10 , G01N2035/00039 , G01N2035/00306 , G01N2035/1032
摘要： A method and automated apparatus for locating and selecting a colony of microorganisms on a culture dish and subjecting the obtained sample to a plurality of downstream tests including a test to identify the microorganism and a test to identify the susceptibility of the microorganism to antibiotics. The method includes the automated steps of locating and selecting a colony of microorganisms on a culture dish; obtaining a sample of the selected colony of microorganisms; preparing a suspension of a sample of microorganisms automatically by submerging the pick tool with the sample in a suspension, after which the pick tool is vibrated in at least the vertical direction to release the sample from the pick tool in the suspension. The turbidity of the suspension is monitored to ensure that the concentration of microorganism in suspension is sufficient so that the suspension is used a source for sample for both identification and antibiotic susceptibility of the microorganisms in the sample. The apparatus and system optionally provides for downstream processing of samples prepared for antibiotic susceptibility testing (AST). Such apparatus includes further processing after inoculation of an AST panel for the AST test. Such further processing includes capping and transferring inoculated panels to AST instrument.
摘要翻译：一种用于在培养皿上定位和选择微生物菌落的方法和自动化装置，并使得到的样品进行多次下游测试，包括鉴定微生物的试验和用于鉴定微生物对抗生素敏感性的试验。该方法包括在培养皿上定位和选择微生物菌落的自动化步骤; 获得所选择的微生物菌落的样品; 通过将采样工具与样品浸没在悬浮液中来自动制备微生物样品的悬浮液，然后拾取工具至少在垂直方向上振动，以将样品从悬浮液中的拾取工具中释放出来。监测悬浮液的浊度，以确保悬浮液中的微生物浓度足以使悬浮液用于样品来源，用于样品中微生物的鉴定和抗生素敏感性。该装置和系统任选提供用于抗生素敏感性试验（AST）制备的样品的下游处理。这样的装置包括在用于AST测试的AST面板接种之后的进一步处理。这种进一步的处理包括将接种的面板封盖并转移到AST仪器。

2. 发明申请

WO2016172532A3 METHOD AND SYSTEM FOR AUTOMATICALLY COUNTING MICROBIAL COLONIES 审中-公开
标题翻译：用于自动计数微生物群的方法和系统
公开(公告)号：WO2016172532A3
公开(公告)日：2016-12-08
申请号：PCT/US2016028919
申请日：2016-04-22
申请人： BD KIESTRA B V , WILES TIMOTHY M
发明人： WILES TIMOTHY M , MARCELPOIL RAPHAEL RODOLPHE
IPC分类号： G06T7/00 , C12M1/34 , G01N33/483 , G06K9/00
CPC分类号： G06T7/0016 , C12M41/36 , C12Q1/02 , G06K9/0014 , G06K9/00147 , G06T2207/10056 , G06T2207/30024
摘要： The present disclosure is directed to an automated method for evaluating growth on plated media, comprising: providing a culture media inoculated with a biological sample (202); incubating the inoculated culture media (204); following incubation, obtaining a first image of the inoculated media at a first time (206); after further incubation, obtaining a second image of the inoculated media at a second time (206); aligning the first image with the second image, such that the coordinates of a pixel in the second image are about the same as the coordinates of a corresponding pixel in the first image; comparing image features of the second image with image features of the first image; classifying image features of the second image as colony candidates based on image feature changes from the first time to the second time; for colony candidates determined to be from a common microorganism in the biological sample inoculated on the culture media, counting said colony candidates;and determining whether the number of counted colonies meets or exceeds the threshold count value stored in memory and indicative of significant growth.
摘要翻译：本公开涉及用于评估电镀培养基上的生长的自动化方法，其包括：提供接种有生物样品（202）的培养基; 培养接种的培养基（204）; 孵育后，在第一次获得接种培养基的第一图像（206）; 在进一步孵育后，在第二次获得接种培养基的第二次图像（206）; 将第一图像与第二图像对准，使得第二图像中的像素的坐标与第一图像中的相应像素的坐标大致相同; 将第二图像的图像特征与第一图像的图像特征进行比较; 基于从第一次到第二次的图像特征变化将第二图像的图像特征分类为殖民地候选; 对于确定来自接种在培养基上的生物样品中的常见微生物的菌落候选物，计数所述菌落候选物;以及确定计数菌落的数目是否满足或超过存储在存储器中并指示显着生长的阈值计数值。

3. 发明申请

WO2016172527A3 COLONY CONTRAST GATHERING 审中-公开
标题翻译：殖民对比
公开(公告)号：WO2016172527A3
公开(公告)日：2017-01-19
申请号：PCT/US2016028913
申请日：2016-04-22
申请人： BD KIESTRA B V , WILES TIMOTHY M
发明人： WILES TIMOTHY M , MARCELPOIL RAPHAEL RODOLPHE
IPC分类号： G06T7/00 , G01N33/483 , G06K9/00
CPC分类号： G06T7/0016 , C12M23/10 , C12M23/22 , C12M41/14 , C12M41/46 , C12M41/48 , G06K9/00127 , G06K9/46 , G06T2207/10056 , G06T2207/10152 , G06T2207/30024
摘要： An imaging system and method for microbial growth detection using differences appearing over time to assess whether microbial growth has occurred for a given sample.
摘要翻译：一种用于微生物生长检测的成像系统和方法，其使用随时间出现的差异来评估给定样品的微生物生长是否发生。

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