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1. 发明申请

WO2007008708A3 METHODS OF PREDICTING HYP-GLYCOSYLATION SITES FOR PROTEINS EXPRESSED AND SECRETED IN PLANT CELLS, AND RELATED METHODS AND PRODUCTS 审中-公开
标题翻译：预测植物细胞中表达和分泌的蛋白质的HYP-糖蛋白化位点的方法及相关方法和产品
公开(公告)号：WO2007008708A3
公开(公告)日：2009-04-23
申请号：PCT/US2006026594
申请日：2006-07-10
申请人： UNIV OHIO , KIELISZEWSKI MARCIA J , XU JIANFENG
发明人： KIELISZEWSKI MARCIA J , XU JIANFENG
IPC分类号： G06F19/00
CPC分类号： C12N15/8257 , G06F19/22
摘要： Proteins with Hyp-glycosylation are more likely to be secreted in plant cells at high levels than those without. Methods are disclosed for the prediction of Pro-hydroxylation and Hyp-glycosylationsites in proteins. Such methods can be used to identify (1) proteins which, without modfication, are predisposed to develop Hyp-glycosylation, if expressed in plant cells, and (2) modifications (especially substitution mutations) which increase the propensity of a protein to develop Hyp-glycosylation, with a view to high level or increased secretion. It is also possible to determine empirically whether a particular protein will undergo Hyp-glycosylation suitable for the desired level of secretion in plant cells. Both modified proteins, and methods for the expression and secretion of predisposed and modified proteins, are claimed.
摘要翻译：具有低糖基化功能的蛋白质在植物细胞中比没有高蛋白质的蛋白质更可能被分泌。公开了用于预测蛋白质中的Pro-羟基化和Hyp-糖基化的方法。这样的方法可以用于鉴定（1）如果在植物细胞中表达，而不改变，发生Hyp糖基化的蛋白质，（2）增加蛋白质发展Hyp的倾向的修饰（特别是取代突变）糖基化，以达到高水平或增加分泌。也可以根据经验确定特定蛋白质是否将进行适合植物细胞中所需水平分泌的Hyp糖基化。要求修饰的蛋白质，以及倾向和修饰的蛋白质的表达和分泌的方法。

2. 发明申请

WO0178503A3 SYNTHETIC GENES FOR PLANT GUMS AND OTHER HYDROXYPROLINE-RICH GLYCOPROTEINS 审中-公开
标题翻译：植物学家和其他羟脯氨酸富含蛋白的合成基因
公开(公告)号：WO0178503A3
公开(公告)日：2003-11-06
申请号：PCT/US0112336
申请日：2001-04-12
申请人： UNIV OHIO , KIELISZEWSKI MARCIA J
发明人： KIELISZEWSKI MARCIA J
IPC分类号： C12N15/09 , A61K38/17 , C07K14/00 , C07K14/415 , C07K19/00 , C12N5/10 , C12N15/29 , C12N15/82 , C12P21/02 , C12N5/04 , A01H5/00 , C12N5/14
CPC分类号： C07K14/415 , C07K2319/00 , C12N15/8241 , C12N15/8242
摘要： A new approach in the field of plant gums is described which presents a new solution to the production of hydroxyproline(Hyp)-rich glycoproteins (HRGPs), repetitive proline-rich proteins (RPRPs) and arabinogalactan-proteins (AGPs). The expression of synthetic genes designed from repetitive peptide sequences of such glycoproteins, including the peptide sequences of gum arabic glycoprotein (GAGP), is taught in host cells, including plant host cells.
摘要翻译：描述了植物胶领域的新方法，其提供了生产富含羟脯氨酸（Hyp）的糖蛋白（HRGP），重复的富含脯氨酸蛋白（RPRP）和阿拉伯半乳聚糖蛋白（AGP）的新解决方案。在宿主细胞（包括植物宿主细胞）中教导了由这种糖蛋白的重复肽序列（包括阿拉伯糖糖蛋白（GAGP）的肽序列）设计的合成基因的表达。

3. 发明申请

WO2007008708A2 METHODS OF PREDICTING HYP-GLYCOSYLATION SITES FOR PROTEINS EXPRESSED AND SECRETED IN PLANT CELLS, AND RELATED METHODS AND PRODUCTS 审中-公开
标题翻译：预测植物细胞中表达和分泌的蛋白质的HYP-糖蛋白化位点的方法及相关方法和产品
公开(公告)号：WO2007008708A2
公开(公告)日：2007-01-18
申请号：PCT/US2006/026594
申请日：2006-07-10
申请人： OHIO UNIVERSITY , KIELISZEWSKI, Marcia, J. , XU, Jianfeng
发明人： KIELISZEWSKI, Marcia, J. , XU, Jianfeng
IPC分类号： G06F19/00
CPC分类号： C12N15/8257 , G06F19/22
摘要： Proteins with Hyp-glycosylation are more likely to be secreted in plant cells at high levels than those without. Methods are disclosed for the prediction of Pro-hydroxylation and Hyp-glycosylationsites in proteins. Such methods can be used to identify (1) proteins which, without modfication, are predisposed to develop Hyp-glycosylation, if expressed in plant cells, and (2) modifications (especially substitution mutations) which increase the propensity of a protein to develop Hyp-glycosylation, with a view to high level or increased secretion. It is also possible to determine empirically whether a particular protein will undergo Hyp-glycosylation suitable for the desired level of secretion in plant cells. Both modified proteins, and methods for the expression and secretion of predisposed and modified proteins, are claimed.
摘要翻译：具有低糖基化功能的蛋白质在植物细胞中比没有高蛋白质的蛋白质更可能被分泌。公开了用于预测蛋白质中的Pro-羟基化和Hyp-糖基化的方法。这样的方法可以用于鉴定（1）如果在植物细胞中表达，而不改变，发生Hyp糖基化的蛋白质，（2）增加蛋白质发展Hyp的倾向的修饰（特别是取代突变）糖基化，以达到高水平或增加分泌。也可以根据经验确定特定蛋白质是否将进行适合植物细胞中所需水平分泌的Hyp糖基化。要求修饰的蛋白质，以及倾向和修饰的蛋白质的表达和分泌的方法。

4. 发明申请

WO2005069845A2 METHODS OF PRODUCING PEPTIDES/PROTEINS IN PLANTS AND PEPTIDES/PROTEINS PRODUCED THEREBY 审中-公开
标题翻译：生产植物和蛋白质/蛋白质的蛋白质/蛋白质的方法
公开(公告)号：WO2005069845A2
公开(公告)日：2005-08-04
申请号：PCT/US2005001160
申请日：2005-01-14
申请人： UNIV OHIO , KIELISZEWSKI MARCIA J , XU JIANFENG , KOPCHICK JOHN , OKADA SHIGERU
发明人： KIELISZEWSKI MARCIA J , XU JIANFENG , KOPCHICK JOHN , OKADA SHIGERU
IPC分类号： C07K14/415 , C07K14/61 , C07K14/765 , C12N15/62 , C12N15/82 , C12P21/00
CPC分类号： C07K14/415 , A61K38/27 , C07K14/56 , C07K14/61 , C07K14/765 , C12N15/62 , C12N15/8257 , C12P21/005
摘要： Methods of increasing the yield in plant expression of recombinant proteins comprising: engineering glycosylation sites into cloned genes or cDNAs for proteins using codons that drive post-translational modifications in plants; and engineering the cloned genes or cDNAs to contain a plant secretory signal sequence that targets the gene products (protein) for secretion. The methods result in increased recombinant glycosylated protein yields. Proteins produced according to these methods are disclosed.
摘要翻译：提高重组蛋白质植物表达产量的方法，其包括：使用驱动植物翻译后修饰的密码子将糖基化位点工程化为克隆基因或蛋白质cDNA; 并对克隆的基因或cDNA进行工程以包含靶向分泌基因产物（蛋白质）的植物分泌信号序列。该方法导致重组糖基化蛋白质产量增加。公开了根据这些方法生产的蛋白质。

5. 发明申请

WO2005069845A3 METHODS OF PRODUCING PEPTIDES/PROTEINS IN PLANTS AND PEPTIDES/PROTEINS PRODUCED THEREBY 审中-公开
公开(公告)号：WO2005069845A3
公开(公告)日：2005-08-04
申请号：PCT/US2005/001160
申请日：2005-01-14
申请人： OHIO UNIVERSITY , KIELISZEWSKI, Marcia, J. , XU, Jianfeng , KOPCHICK, John , OKADA, Shigeru
发明人： KIELISZEWSKI, Marcia, J. , XU, Jianfeng , KOPCHICK, John , OKADA, Shigeru
IPC分类号： C07K19/00 , C07H21/04 , C07H21/02 , C07H21/00 , C12N15/82
摘要： Methods of increasing the yield in plant expression of recombinant proteins comprising: engineering glycosylation sites into cloned genes or cDNAs for proteins using codons that drive post-translational modifications in plants; and engineering the cloned genes or cDNAs to contain a plant secretory signal sequence that targets the gene products (protein) for secretion. The methods result in increased recombinant glycosylated protein yields. Proteins produced according to these methods are disclosed.

6. 发明申请

WO2004094590A3 SYNTHETIC GENES FOR PLANT GUMS AND OTHER HYDROXYPROLINE-RICH GLYCOPROTEINS 审中-公开
标题翻译：植物学家和其他羟脯氨酸富含蛋白酶的合成基因
公开(公告)号：WO2004094590A3
公开(公告)日：2005-05-19
申请号：PCT/US2004011174
申请日：2004-04-13
申请人： UNIV OHIO , KIELISZEWSKI MARCIA J
发明人： KIELISZEWSKI MARCIA J
IPC分类号： A61K38/14 , C07H21/04 , C07K9/00 , C07K14/415 , C12N20060101 , C12N5/04 , C12N15/29 , C12N15/82
CPC分类号： C07K14/415 , C07K2319/00 , C12N15/8241 , C12N15/8242
摘要： A new approach in the field of plant gums is described which presents a new solution to the production of hydroxyproline(Hyp)-rich glycoproteins (HRGPs), repetitive proline-rich proteins (RPRPs) and arabinogalactan-proteins (AGPs). The expression of synthetic genes designed from repetitive peptide sequences of such glycoproteins, including the peptide sequences of gum arabic glycoprotein (GAGP), is taught in host cells, including plant host cells.
摘要翻译：描述了植物胶领域的新方法，其提供了生产富含羟脯氨酸（Hyp）的糖蛋白（HRGP），重复富含脯氨酸蛋白（RPRP）和阿拉伯半乳聚糖 - 蛋白（AGP）的新解决方案。在宿主细胞（包括植物宿主细胞）中教导了由这种糖蛋白的重复肽序列（包括阿拉伯胶糖蛋白（GAGP）的肽序列）设计的合成基因的表达。

7. 发明申请

WO2008008766A3 CO-EXPRESSION OF PROLINE HYDROXYLASES TO FACILITATE HYP-GLYCOSYLATION OF PROTEINS EXPRESSED AND SECRETED IN PLANT CELLS 审中-公开
标题翻译：共表达丙氨酸羟基来促进植物细胞中表达和分泌的蛋白质的高糖蛋白化
公开(公告)号：WO2008008766A3
公开(公告)日：2008-11-20
申请号：PCT/US2007073137
申请日：2007-07-10
申请人： UNIV OHIO , KIELISZEWSKI MARCIA , XU JIANFENG , COOPER IVER P
发明人： KIELISZEWSKI MARCIA , XU JIANFENG , COOPER IVER P
IPC分类号： C12N15/82
CPC分类号： C12N15/8257 , C07K14/78 , C12N9/0071 , C12P21/005
摘要： Plant cells contain particular proline hydroxylases whose specificity determines which prolines, in a protein expressed therein, are hydroxylated. The resulting hydroxyprolines, in turn, my be glycosylated. It is the presence of glycosylated hydroxyprolines which is the most important determinant of the degree of secretion of the protein. One may modify the proline hydroxylation capabilities of the plant cell by co-expressing in that cell at least one exogeneous proline hyrdoxylase which will hydroxylate at least one proline site in the protein which is not hydroxylated by the endogeneous proline hydroxylase, but which, if hydroxylated, can than be glycosylated by the plant cell, resulting in increased Hyp-glycosylation. Moreover, even if the co-expressed exogenous proline hydroxylase doesn't achieve the desired level of Hyp-glycosylation in the native protein, such co-expression may still be used in conjunction with modification of the protein itself to increase the number and/or the likelihood of Hyp-glycosylation sites.
摘要翻译：植物细胞含有特定的脯氨酸羟化酶，其特异性决定了其中表达的蛋白质中的哪些脯氨酸被羟基化。所得的羟基脯氨酸又被我糖基化。糖化羟脯氨酸的存在是蛋白质分泌程度最重要的决定因素。人们可以通过在该细胞中共表达至少一种外源脯氨酸脱乙酰酶，来改变植物细胞的脯氨酸羟基化能力，该外源脯氨酸脱乙酰酶能使蛋白质中的至少一个脯氨酸位点羟基化而不被内源脯氨酸羟化酶羟基化，但如果羟基化，可以被植物细胞糖基化，导致Hyp-糖基化的增加。此外，即使共表达的外源脯氨酸羟化酶在天然蛋白质中没有达到所需的Hyp糖基化水平，这种共表达仍然可以与蛋白质本身的修饰结合使用以增加数量和/或低糖基化位点的可能性。

8. 发明申请

WO2005110015A2 CROSS-LINKABLE GLYCOPROTEINS AND METHODS OF MAKING THE SAME 审中-公开
标题翻译：可交联的糖蛋白及其制备方法
公开(公告)号：WO2005110015A2
公开(公告)日：2005-11-24
申请号：PCT/US2005/013252
申请日：2005-04-19
申请人： OHIO UNIVERSITY , KIELISZEWSKI, Marcia, J. , HELD, Michael , TAN, Li
发明人： KIELISZEWSKI, Marcia, J. , HELD, Michael , TAN, Li
IPC分类号： C07K14/00
CPC分类号： C07K14/00 , C07K14/415
摘要： Non-naturally occurring peptides/polypeptides/proteins comprising the crosslinking motif, Tyr-X-Tyr, wherein X is any amino acid, and methods of preparing the same.
摘要翻译：包含交联基序Tyr-X-Tyr（其中X是任何氨基酸）的非天然存在的肽/多肽/蛋白质及其制备方法。

9. 发明申请

WO2008008766A2 CO-EXPRESSION OF PROLINE HYDROXYLASES TO FACILITATE HYP-GLYCOSYLATION OF PROTEINS EXPRESSED AND SECRETED IN PLANT CELLS 审中-公开
标题翻译：脯氨酸羟化酶共表达促进植物细胞表达和分泌蛋白质的HYP糖基化
公开(公告)号：WO2008008766A2
公开(公告)日：2008-01-17
申请号：PCT/US2007/073137
申请日：2007-07-10
申请人： OHIO UNIVERSITY , KIELISZEWSKI, Marcia , XU, Jianfeng , COOPER, Iver P.
发明人： KIELISZEWSKI, Marcia , XU, Jianfeng , COOPER, Iver P.
IPC分类号： A01H5/00
CPC分类号： C12N15/8257 , C07K14/78 , C12N9/0071 , C12P21/005
摘要： Plant cells contain particular proline hydroxylases whose specificity determines which prolines, in a protein expressed therein, are hydroxylated. The resulting hydroxyprolines, in turn, my be glycosylated. It is the presence of glycosylated hydroxyprolines which is the most important determinant of the degree of secretion of the protein. One may modify the proline hydroxylation capabilities of the plant cell by co-expressing in that cell at least one exogeneous proline hyrdoxylase which will hydroxylate at least one proline site in the protein which is not hydroxylated by the endogeneous proline hydroxylase, but which, if hydroxylated, can than be glycosylated by the plant cell, resulting in increased Hyp-glycosylation. Moreover, even if the co-expressed exogenous proline hydroxylase doesn't achieve the desired level of Hyp-glycosylation in the native protein, such co-expression may still be used in conjunction with modification of the protein itself to increase the number and/or the likelihood of Hyp-glycosylation sites.
摘要翻译：植物细胞含有特定的脯氨酸羟化酶，其特异性决定了在其中表达的蛋白质中哪种脯氨酸被羟基化。得到的羟脯氨酸反过来被糖基化。糖基化羟脯氨酸是蛋白质分泌程度的最重要决定因素。人们可以通过在该细胞中共表达至少一种外源脯氨酸羟化酶来修饰植物细胞的脯氨酸羟基化能力，所述外源脯氨酸羟化酶将羟化蛋白质中的至少一个脯氨酸位点，所述脯氨酸位点不被内源脯氨酸羟化酶羟化，，可以被植物细胞糖基化，导致Hyp-糖基化增加。而且，即使共表达的外源脯氨酸羟化酶在天然蛋白质中没有达到希望的Hyp-糖基化水平，这种共表达仍然可以与蛋白质本身的修饰结合使用以增加蛋白质的数量和/或 Hyp-糖基化位点的可能性。

10. 发明申请

WO2005110015A3 CROSS-LINKABLE GLYCOPROTEINS AND METHODS OF MAKING THE SAME 审中-公开
标题翻译：可交联的糖蛋白及其制备方法
公开(公告)号：WO2005110015A3
公开(公告)日：2006-12-21
申请号：PCT/US2005013252
申请日：2005-04-19
申请人： UNIV OHIO , KIELISZEWSKI MARCIA J , HELD MICHAEL , TAN LI
发明人： KIELISZEWSKI MARCIA J , HELD MICHAEL , TAN LI
IPC分类号： C07K14/00
CPC分类号： C07K14/00 , C07K14/415
摘要： Non-naturally occurring peptides/polypeptides/proteins comprising the crosslinking motif, Tyr-X-Tyr, wherein X is any amino acid, and methods of preparing the same.
摘要翻译：包含交联基序Tyr-X-Tyr（其中X是任何氨基酸）的非天然存在的肽/多肽/蛋白质及其制备方法。

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