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1. 发明申请

WO2007008708A3 METHODS OF PREDICTING HYP-GLYCOSYLATION SITES FOR PROTEINS EXPRESSED AND SECRETED IN PLANT CELLS, AND RELATED METHODS AND PRODUCTS 审中-公开
标题翻译：预测植物细胞中表达和分泌的蛋白质的HYP-糖蛋白化位点的方法及相关方法和产品
公开(公告)号：WO2007008708A3
公开(公告)日：2009-04-23
申请号：PCT/US2006026594
申请日：2006-07-10
申请人： UNIV OHIO , KIELISZEWSKI MARCIA J , XU JIANFENG
发明人： KIELISZEWSKI MARCIA J , XU JIANFENG
IPC分类号： G06F19/00
CPC分类号： C12N15/8257 , G06F19/22
摘要： Proteins with Hyp-glycosylation are more likely to be secreted in plant cells at high levels than those without. Methods are disclosed for the prediction of Pro-hydroxylation and Hyp-glycosylationsites in proteins. Such methods can be used to identify (1) proteins which, without modfication, are predisposed to develop Hyp-glycosylation, if expressed in plant cells, and (2) modifications (especially substitution mutations) which increase the propensity of a protein to develop Hyp-glycosylation, with a view to high level or increased secretion. It is also possible to determine empirically whether a particular protein will undergo Hyp-glycosylation suitable for the desired level of secretion in plant cells. Both modified proteins, and methods for the expression and secretion of predisposed and modified proteins, are claimed.
摘要翻译：具有低糖基化功能的蛋白质在植物细胞中比没有高蛋白质的蛋白质更可能被分泌。公开了用于预测蛋白质中的Pro-羟基化和Hyp-糖基化的方法。这样的方法可以用于鉴定（1）如果在植物细胞中表达，而不改变，发生Hyp糖基化的蛋白质，（2）增加蛋白质发展Hyp的倾向的修饰（特别是取代突变）糖基化，以达到高水平或增加分泌。也可以根据经验确定特定蛋白质是否将进行适合植物细胞中所需水平分泌的Hyp糖基化。要求修饰的蛋白质，以及倾向和修饰的蛋白质的表达和分泌的方法。

2. 发明申请

WO0178503A3 SYNTHETIC GENES FOR PLANT GUMS AND OTHER HYDROXYPROLINE-RICH GLYCOPROTEINS 审中-公开
标题翻译：植物学家和其他羟脯氨酸富含蛋白的合成基因
公开(公告)号：WO0178503A3
公开(公告)日：2003-11-06
申请号：PCT/US0112336
申请日：2001-04-12
申请人： UNIV OHIO , KIELISZEWSKI MARCIA J
发明人： KIELISZEWSKI MARCIA J
IPC分类号： C12N15/09 , A61K38/17 , C07K14/00 , C07K14/415 , C07K19/00 , C12N5/10 , C12N15/29 , C12N15/82 , C12P21/02 , C12N5/04 , A01H5/00 , C12N5/14
CPC分类号： C07K14/415 , C07K2319/00 , C12N15/8241 , C12N15/8242
摘要： A new approach in the field of plant gums is described which presents a new solution to the production of hydroxyproline(Hyp)-rich glycoproteins (HRGPs), repetitive proline-rich proteins (RPRPs) and arabinogalactan-proteins (AGPs). The expression of synthetic genes designed from repetitive peptide sequences of such glycoproteins, including the peptide sequences of gum arabic glycoprotein (GAGP), is taught in host cells, including plant host cells.
摘要翻译：描述了植物胶领域的新方法，其提供了生产富含羟脯氨酸（Hyp）的糖蛋白（HRGP），重复的富含脯氨酸蛋白（RPRP）和阿拉伯半乳聚糖蛋白（AGP）的新解决方案。在宿主细胞（包括植物宿主细胞）中教导了由这种糖蛋白的重复肽序列（包括阿拉伯糖糖蛋白（GAGP）的肽序列）设计的合成基因的表达。

3. 发明申请

WO2007008708A2 METHODS OF PREDICTING HYP-GLYCOSYLATION SITES FOR PROTEINS EXPRESSED AND SECRETED IN PLANT CELLS, AND RELATED METHODS AND PRODUCTS 审中-公开
标题翻译：预测植物细胞中表达和分泌的蛋白质的HYP-糖蛋白化位点的方法及相关方法和产品
公开(公告)号：WO2007008708A2
公开(公告)日：2007-01-18
申请号：PCT/US2006/026594
申请日：2006-07-10
申请人： OHIO UNIVERSITY , KIELISZEWSKI, Marcia, J. , XU, Jianfeng
发明人： KIELISZEWSKI, Marcia, J. , XU, Jianfeng
IPC分类号： G06F19/00
CPC分类号： C12N15/8257 , G06F19/22
摘要： Proteins with Hyp-glycosylation are more likely to be secreted in plant cells at high levels than those without. Methods are disclosed for the prediction of Pro-hydroxylation and Hyp-glycosylationsites in proteins. Such methods can be used to identify (1) proteins which, without modfication, are predisposed to develop Hyp-glycosylation, if expressed in plant cells, and (2) modifications (especially substitution mutations) which increase the propensity of a protein to develop Hyp-glycosylation, with a view to high level or increased secretion. It is also possible to determine empirically whether a particular protein will undergo Hyp-glycosylation suitable for the desired level of secretion in plant cells. Both modified proteins, and methods for the expression and secretion of predisposed and modified proteins, are claimed.
摘要翻译：具有低糖基化功能的蛋白质在植物细胞中比没有高蛋白质的蛋白质更可能被分泌。公开了用于预测蛋白质中的Pro-羟基化和Hyp-糖基化的方法。这样的方法可以用于鉴定（1）如果在植物细胞中表达，而不改变，发生Hyp糖基化的蛋白质，（2）增加蛋白质发展Hyp的倾向的修饰（特别是取代突变）糖基化，以达到高水平或增加分泌。也可以根据经验确定特定蛋白质是否将进行适合植物细胞中所需水平分泌的Hyp糖基化。要求修饰的蛋白质，以及倾向和修饰的蛋白质的表达和分泌的方法。

4. 发明申请

WO2005069845A2 METHODS OF PRODUCING PEPTIDES/PROTEINS IN PLANTS AND PEPTIDES/PROTEINS PRODUCED THEREBY 审中-公开
标题翻译：生产植物和蛋白质/蛋白质的蛋白质/蛋白质的方法
公开(公告)号：WO2005069845A2
公开(公告)日：2005-08-04
申请号：PCT/US2005001160
申请日：2005-01-14
申请人： UNIV OHIO , KIELISZEWSKI MARCIA J , XU JIANFENG , KOPCHICK JOHN , OKADA SHIGERU
发明人： KIELISZEWSKI MARCIA J , XU JIANFENG , KOPCHICK JOHN , OKADA SHIGERU
IPC分类号： C07K14/415 , C07K14/61 , C07K14/765 , C12N15/62 , C12N15/82 , C12P21/00
CPC分类号： C07K14/415 , A61K38/27 , C07K14/56 , C07K14/61 , C07K14/765 , C12N15/62 , C12N15/8257 , C12P21/005
摘要： Methods of increasing the yield in plant expression of recombinant proteins comprising: engineering glycosylation sites into cloned genes or cDNAs for proteins using codons that drive post-translational modifications in plants; and engineering the cloned genes or cDNAs to contain a plant secretory signal sequence that targets the gene products (protein) for secretion. The methods result in increased recombinant glycosylated protein yields. Proteins produced according to these methods are disclosed.
摘要翻译：提高重组蛋白质植物表达产量的方法，其包括：使用驱动植物翻译后修饰的密码子将糖基化位点工程化为克隆基因或蛋白质cDNA; 并对克隆的基因或cDNA进行工程以包含靶向分泌基因产物（蛋白质）的植物分泌信号序列。该方法导致重组糖基化蛋白质产量增加。公开了根据这些方法生产的蛋白质。

5. 发明申请

WO2005069845A3 METHODS OF PRODUCING PEPTIDES/PROTEINS IN PLANTS AND PEPTIDES/PROTEINS PRODUCED THEREBY 审中-公开
公开(公告)号：WO2005069845A3
公开(公告)日：2005-08-04
申请号：PCT/US2005/001160
申请日：2005-01-14
申请人： OHIO UNIVERSITY , KIELISZEWSKI, Marcia, J. , XU, Jianfeng , KOPCHICK, John , OKADA, Shigeru
发明人： KIELISZEWSKI, Marcia, J. , XU, Jianfeng , KOPCHICK, John , OKADA, Shigeru
IPC分类号： C07K19/00 , C07H21/04 , C07H21/02 , C07H21/00 , C12N15/82
摘要： Methods of increasing the yield in plant expression of recombinant proteins comprising: engineering glycosylation sites into cloned genes or cDNAs for proteins using codons that drive post-translational modifications in plants; and engineering the cloned genes or cDNAs to contain a plant secretory signal sequence that targets the gene products (protein) for secretion. The methods result in increased recombinant glycosylated protein yields. Proteins produced according to these methods are disclosed.

6. 发明申请

WO2004094590A3 SYNTHETIC GENES FOR PLANT GUMS AND OTHER HYDROXYPROLINE-RICH GLYCOPROTEINS 审中-公开
标题翻译：植物学家和其他羟脯氨酸富含蛋白酶的合成基因
公开(公告)号：WO2004094590A3
公开(公告)日：2005-05-19
申请号：PCT/US2004011174
申请日：2004-04-13
申请人： UNIV OHIO , KIELISZEWSKI MARCIA J
发明人： KIELISZEWSKI MARCIA J
IPC分类号： A61K38/14 , C07H21/04 , C07K9/00 , C07K14/415 , C12N20060101 , C12N5/04 , C12N15/29 , C12N15/82
CPC分类号： C07K14/415 , C07K2319/00 , C12N15/8241 , C12N15/8242
摘要： A new approach in the field of plant gums is described which presents a new solution to the production of hydroxyproline(Hyp)-rich glycoproteins (HRGPs), repetitive proline-rich proteins (RPRPs) and arabinogalactan-proteins (AGPs). The expression of synthetic genes designed from repetitive peptide sequences of such glycoproteins, including the peptide sequences of gum arabic glycoprotein (GAGP), is taught in host cells, including plant host cells.
摘要翻译：描述了植物胶领域的新方法，其提供了生产富含羟脯氨酸（Hyp）的糖蛋白（HRGP），重复富含脯氨酸蛋白（RPRP）和阿拉伯半乳聚糖 - 蛋白（AGP）的新解决方案。在宿主细胞（包括植物宿主细胞）中教导了由这种糖蛋白的重复肽序列（包括阿拉伯胶糖蛋白（GAGP）的肽序列）设计的合成基因的表达。

7. 发明申请

WO2005110015A2 CROSS-LINKABLE GLYCOPROTEINS AND METHODS OF MAKING THE SAME 审中-公开
标题翻译：可交联的糖蛋白及其制备方法
公开(公告)号：WO2005110015A2
公开(公告)日：2005-11-24
申请号：PCT/US2005/013252
申请日：2005-04-19
申请人： OHIO UNIVERSITY , KIELISZEWSKI, Marcia, J. , HELD, Michael , TAN, Li
发明人： KIELISZEWSKI, Marcia, J. , HELD, Michael , TAN, Li
IPC分类号： C07K14/00
CPC分类号： C07K14/00 , C07K14/415
摘要： Non-naturally occurring peptides/polypeptides/proteins comprising the crosslinking motif, Tyr-X-Tyr, wherein X is any amino acid, and methods of preparing the same.
摘要翻译：包含交联基序Tyr-X-Tyr（其中X是任何氨基酸）的非天然存在的肽/多肽/蛋白质及其制备方法。

8. 发明申请

WO2005110015A3 CROSS-LINKABLE GLYCOPROTEINS AND METHODS OF MAKING THE SAME 审中-公开
标题翻译：可交联的糖蛋白及其制备方法
公开(公告)号：WO2005110015A3
公开(公告)日：2006-12-21
申请号：PCT/US2005013252
申请日：2005-04-19
申请人： UNIV OHIO , KIELISZEWSKI MARCIA J , HELD MICHAEL , TAN LI
发明人： KIELISZEWSKI MARCIA J , HELD MICHAEL , TAN LI
IPC分类号： C07K14/00
CPC分类号： C07K14/00 , C07K14/415
摘要： Non-naturally occurring peptides/polypeptides/proteins comprising the crosslinking motif, Tyr-X-Tyr, wherein X is any amino acid, and methods of preparing the same.
摘要翻译：包含交联基序Tyr-X-Tyr（其中X是任何氨基酸）的非天然存在的肽/多肽/蛋白质及其制备方法。

9. 发明申请

WO2004094590A2 SYNTHETIC GENES FOR PLANT GUMS AND OTHER HYDROXYPROLINE-RICH GLYCOPROTEINS 审中-公开
标题翻译：植物学家和其他羟脯氨酸富含蛋白的合成基因
公开(公告)号：WO2004094590A2
公开(公告)日：2004-11-04
申请号：PCT/US2004/011174
申请日：2004-04-13
申请人： OHIO UNIVERSITY , KIELISZEWSKI, Marcia, J.
发明人： KIELISZEWSKI, Marcia, J.
IPC分类号： C12N
CPC分类号： C07K14/415 , C07K2319/00 , C12N15/8241 , C12N15/8242
摘要： A new approach in the field of plant gums is described which presents a new solution to the production of hydroxyproline(Hyp)-rich glycoproteins (HRGPs), repetitive proline-rich proteins (RPRPs) and arabinogalactan-proteins (AGPs). The expression of synthetic genes designed from repetitive peptide sequences of such glycoproteins, including the peptide sequences of gum arabic glycoprotein (GAGP), is taught in host cells, including plant host cells.
摘要翻译：描述了植物胶领域的新方法，其提供了生产富含羟脯氨酸（Hyp）的糖蛋白（HRGP），重复的富含脯氨酸蛋白（RPRP）和阿拉伯半乳聚糖蛋白（AGP）的新解决方案。在宿主细胞（包括植物宿主细胞）中教导了由这种糖蛋白的重复肽序列（包括阿拉伯糖糖蛋白（GAGP）的肽序列）设计的合成基因的表达。

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