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1. 发明申请

WO2008008766A2 CO-EXPRESSION OF PROLINE HYDROXYLASES TO FACILITATE HYP-GLYCOSYLATION OF PROTEINS EXPRESSED AND SECRETED IN PLANT CELLS 审中-公开
标题翻译：脯氨酸羟化酶共表达促进植物细胞表达和分泌蛋白质的HYP糖基化
公开(公告)号：WO2008008766A2
公开(公告)日：2008-01-17
申请号：PCT/US2007/073137
申请日：2007-07-10
申请人： OHIO UNIVERSITY , KIELISZEWSKI, Marcia , XU, Jianfeng , COOPER, Iver P.
发明人： KIELISZEWSKI, Marcia , XU, Jianfeng , COOPER, Iver P.
IPC分类号： A01H5/00
CPC分类号： C12N15/8257 , C07K14/78 , C12N9/0071 , C12P21/005
摘要： Plant cells contain particular proline hydroxylases whose specificity determines which prolines, in a protein expressed therein, are hydroxylated. The resulting hydroxyprolines, in turn, my be glycosylated. It is the presence of glycosylated hydroxyprolines which is the most important determinant of the degree of secretion of the protein. One may modify the proline hydroxylation capabilities of the plant cell by co-expressing in that cell at least one exogeneous proline hyrdoxylase which will hydroxylate at least one proline site in the protein which is not hydroxylated by the endogeneous proline hydroxylase, but which, if hydroxylated, can than be glycosylated by the plant cell, resulting in increased Hyp-glycosylation. Moreover, even if the co-expressed exogenous proline hydroxylase doesn't achieve the desired level of Hyp-glycosylation in the native protein, such co-expression may still be used in conjunction with modification of the protein itself to increase the number and/or the likelihood of Hyp-glycosylation sites.
摘要翻译：植物细胞含有特定的脯氨酸羟化酶，其特异性决定了在其中表达的蛋白质中哪种脯氨酸被羟基化。得到的羟脯氨酸反过来被糖基化。糖基化羟脯氨酸是蛋白质分泌程度的最重要决定因素。人们可以通过在该细胞中共表达至少一种外源脯氨酸羟化酶来修饰植物细胞的脯氨酸羟基化能力，所述外源脯氨酸羟化酶将羟化蛋白质中的至少一个脯氨酸位点，所述脯氨酸位点不被内源脯氨酸羟化酶羟化，，可以被植物细胞糖基化，导致Hyp-糖基化增加。而且，即使共表达的外源脯氨酸羟化酶在天然蛋白质中没有达到希望的Hyp-糖基化水平，这种共表达仍然可以与蛋白质本身的修饰结合使用以增加蛋白质的数量和/或 Hyp-糖基化位点的可能性。

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