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    • 7. 发明申请
    • METHOD OF IDENTIFYING NUCLEIC ACID-CONTAINING OBJECT
    • 识别含核酸物​​体的方法
    • WO2012124968A2
    • 2012-09-20
    • PCT/KR2012001815
    • 2012-03-13
    • BIONEER CORPPARK HAN OHCHOI JUNG YOUNGHAN EUN SUSONG GU YOUNGJANG WON SEOK
    • PARK HAN OHCHOI JUNG YOUNGHAN EUN SUSONG GU YOUNGJANG WON SEOK
    • C12Q1/68C12N15/11G01N33/52
    • C12Q1/6816C12Q1/6818C12Q2521/327C12Q2525/121C12Q2563/185
    • The present invention relates to a method of identifying nucleic acid-containing object, more precisely a method of identifying nucleic acid-containing object which comprises the following steps: (1) preparing nucleic acid-containing object having the nucleotide sequence complementary to the nucleotide sequence of RNA-dual probe; (2) reacting the nucleic acid included in the object with the buffer containing the RNA-dual probe conjugated with a reporter and a quencher respectively and RNase; and (3) detecting fluorescence generated from the reporter. The method of identifying an object of the present invention provides labeling sensitivity 100 times as high as that of the conventional method using sequencing or labeling with fluorescent materials, takes advantages of shorter analysis time, facilitates different labeling on a variety of products according to fluorescent materials, and makes possible unlimited product administration by product group and batch in real production process by differentiating the nucleotide sequence of each oligonucleotide.
    • 本发明涉及一种鉴定含核酸对象的方法,更准确地说是一种鉴定含核酸对象的方法,该方法包括以下步骤:(1)制备具有与该核苷酸序列互补的核苷酸序列的含核酸对象 RNA双重探针; (2)使包含在所述物体中的核酸与含有分别与报道分子和猝灭剂结合的RNA双重探针和RNase的缓冲液反应; 和(3)检测由报道分子产生的荧光。 本发明的识别目标的方法提供了与使用荧光材料测序或标记的常规方法相比高100倍的标记灵敏度,利用了更短的分析时间,促进了根据荧光材料在各种产品上的不同标记 并且通过区分每种寡核苷酸的核苷酸序列,可以在实际生产过程中通过产品组和批次实现无限制的产品管理。