专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2012124968A3 METHOD OF IDENTIFYING NUCLEIC ACID-CONTAINING OBJECT 审中-公开
公开(公告)号：WO2012124968A3
公开(公告)日：2012-09-20
申请号：PCT/KR2012/001815
申请日：2012-03-13
申请人： BIONEER CORPORATION , PARK, Han Oh , CHOI, Jung Young , HAN, Eun Su , SONG, Gu Young , JANG, Won Seok
发明人： PARK, Han Oh , CHOI, Jung Young , HAN, Eun Su , SONG, Gu Young , JANG, Won Seok
IPC分类号： C12Q1/68 , C12N15/11 , G01N33/52
摘要： The present invention relates to a method of identifying nucleic acid-containing object, more precisely a method of identifying nucleic acid-containing object which comprises the following steps: (1) preparing nucleic acid-containing object having the nucleotide sequence complementary to the nucleotide sequence of RNA-dual probe; (2) reacting the nucleic acid included in the object with the buffer containing the RNA-dual probe conjugated with a reporter and a quencher respectively and RNase; and (3) detecting fluorescence generated from the reporter. The method of identifying an object of the present invention provides labeling sensitivity 100 times as high as that of the conventional method using sequencing or labeling with fluorescent materials, takes advantages of shorter analysis time, facilitates different labeling on a variety of products according to fluorescent materials, and makes possible unlimited product administration by product group and batch in real production process by differentiating the nucleotide sequence of each oligonucleotide.

2. 发明申请

WO2011025329A3 DETECTING METHODS OF DNA AMPLIFICATION USING NEW INTERCALATING AGENT 审中-公开
公开(公告)号：WO2011025329A3
公开(公告)日：2011-03-03
申请号：PCT/KR2010/005840
申请日：2010-08-30
申请人： BIONEER CORPORATION , LEE, Jun Hee , PARK, Hwang Seo , HAN, Eun Su , LEE, Jae Don , PARK, Han Oh
发明人： LEE, Jun Hee , PARK, Hwang Seo , HAN, Eun Su , LEE, Jae Don , PARK, Han Oh
IPC分类号： C09K11/06
摘要： Disclosed are a fluorescent composition for staining DNA and a method for measuring DNA amplification using the same. More particularly, disclosed are a novel fluorescent compound that emits light by intercalating with DNA and a method for measuring DNA amplification using the same. The novel fluorescent compound is advantageous in that, due to high sensitivity, target-specific amplification products by real-time polymerase chain reaction(PCR) can be accurately quantified without affecting the PCR reaction. In addition, the compound is unharmful to the human body, can be prepared at low cost, and allows monitoring of various DNA and RNA reactions in vitro or in vivo.

3. 发明申请

WO2012124968A2 METHOD OF IDENTIFYING NUCLEIC ACID-CONTAINING OBJECT 审中-公开
标题翻译：识别含核酸物体的方法
公开(公告)号：WO2012124968A2
公开(公告)日：2012-09-20
申请号：PCT/KR2012001815
申请日：2012-03-13
申请人： BIONEER CORP , PARK HAN OH , CHOI JUNG YOUNG , HAN EUN SU , SONG GU YOUNG , JANG WON SEOK
发明人： PARK HAN OH , CHOI JUNG YOUNG , HAN EUN SU , SONG GU YOUNG , JANG WON SEOK
IPC分类号： C12Q1/68 , C12N15/11 , G01N33/52
CPC分类号： C12Q1/6816 , C12Q1/6818 , C12Q2521/327 , C12Q2525/121 , C12Q2563/185
摘要： The present invention relates to a method of identifying nucleic acid-containing object, more precisely a method of identifying nucleic acid-containing object which comprises the following steps: (1) preparing nucleic acid-containing object having the nucleotide sequence complementary to the nucleotide sequence of RNA-dual probe; (2) reacting the nucleic acid included in the object with the buffer containing the RNA-dual probe conjugated with a reporter and a quencher respectively and RNase; and (3) detecting fluorescence generated from the reporter. The method of identifying an object of the present invention provides labeling sensitivity 100 times as high as that of the conventional method using sequencing or labeling with fluorescent materials, takes advantages of shorter analysis time, facilitates different labeling on a variety of products according to fluorescent materials, and makes possible unlimited product administration by product group and batch in real production process by differentiating the nucleotide sequence of each oligonucleotide.
摘要翻译：本发明涉及一种鉴定含核酸对象的方法，更准确地说是一种鉴定含核酸对象的方法，该方法包括以下步骤：（1）制备具有与该核苷酸序列互补的核苷酸序列的含核酸对象 RNA双重探针; （2）使包含在所述物体中的核酸与含有分别与报道分子和猝灭剂结合的RNA双重探针和RNase的缓冲液反应; 和（3）检测由报道分子产生的荧光。本发明的识别目标的方法提供了与使用荧光材料测序或标记的常规方法相比高100倍的标记灵敏度，利用了更短的分析时间，促进了根据荧光材料在各种产品上的不同标记并且通过区分每种寡核苷酸的核苷酸序列，可以在实际生产过程中通过产品组和批次实现无限制的产品管理。

4. 发明申请

WO2011025329A2 DETECTING METHODS OF DNA AMPLIFICATION USING NEW INTERCALATING AGENT 审中-公开
标题翻译：用新的内标剂检测DNA扩增的方法
公开(公告)号：WO2011025329A2
公开(公告)日：2011-03-03
申请号：PCT/KR2010005840
申请日：2010-08-30
申请人： BIONEER CORP , LEE JUN HEE , PARK HWANG SEO , HAN EUN SU , LEE JAE DON , PARK HAN OH
发明人： LEE JUN HEE , PARK HWANG SEO , HAN EUN SU , LEE JAE DON , PARK HAN OH
IPC分类号： C09K11/06
CPC分类号： C09K11/06 , C09K2211/1007 , C09K2211/1011 , C09K2211/1014 , C09K2211/1029 , C09K2211/1037 , C12Q1/6841 , G01N1/30 , C12Q2565/629 , C12Q2565/631
摘要： Disclosed are a fluorescent composition for staining DNA and a method for measuring DNA amplification using the same. More particularly, disclosed are a novel fluorescent compound that emits light by intercalating with DNA and a method for measuring DNA amplification using the same. The novel fluorescent compound is advantageous in that, due to high sensitivity, target-specific amplification products by real-time polymerase chain reaction(PCR) can be accurately quantified without affecting the PCR reaction. In addition, the compound is unharmful to the human body, can be prepared at low cost, and allows monitoring of various DNA and RNA reactions in vitro or in vivo.
摘要翻译：公开了用于染色DNA的荧光组合物和使用其的DNA扩增测量方法。更具体地说，公开了通过插入DNA而发光的新型荧光化合物和使用其测量DNA扩增的方法。该新型荧光化合物的优点在于，由于高灵敏度，通过实时聚合酶链反应（PCR）的目标特异性扩增产物可以准确定量而不影响PCR反应。此外，该化合物对人体无害，可以以低成本制备，并且允许在体外或体内监测各种DNA和RNA反应。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式