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    • 1. 发明申请
    • PROCESS FOR PRODUCING ALCOHOL FROM STARCH
    • 从淀粉生产酒精的方法
    • WO03016525A9
    • 2003-10-16
    • PCT/JP0208234
    • 2002-08-12
    • KANSAI CHEM ENGFUKUDA HIDEKIKONDO AKIHIKOTANAKA ATSUOUEDA MITSUYOSHISATOH EIICHI
    • FUKUDA HIDEKIKONDO AKIHIKOTANAKA ATSUOUEDA MITSUYOSHISATOH EIICHI
    • C12N1/15C12N15/81C12P7/06C12N15/09
    • C12P7/06C12N15/81Y02E50/17
    • A plasmid having been constructed so as to present glucoamylase on its surface layer and secret alpha-amylase is integrated into a yeast and then the yeast is grown using starch as a substrate. Thus, al alcohol can be efficiently produced by fermentation directly from the starch. This yeast is superior in alcohol fermentability to a yeast presenting glucoamylase and alpha-amylase on the cell surface layer, a yeast presenting alpha-amylase on the cell surface layer and secreting glucoamylase, and a yeast presenting glucoamylase on the surface layer. Using alpha-amylase originating in Streptococcus bovis, moreover, ethanol can be directly produced from uncooked starch. It is also possible to employ a yeast which presents on the cell surface layer glucoamylase and alpha-amylase as a fused protein with the agglutination function domain of a sugar chain-binding protein.
    • 已经构建成在其表面层和秘密的α-淀粉酶上呈现葡糖淀粉酶的质粒被整合到酵母中,然后使用淀粉作为底物生长酵母。 因此,可以通过直接从淀粉发酵来有效地制备醇。 该酵母在酒精发酵能力上优于在细胞表面层上呈现葡糖淀粉酶和α-淀粉酶的酵母,在细胞表面层上呈现α-淀粉酶并分泌葡糖淀粉酶的酵母,以及在表面层上呈现葡糖淀粉酶的酵母。 此外,使用源自牛链球菌的α-淀粉酶,可以从未煮过的淀粉直接生产乙醇。 也可以使用在细胞表面层葡萄糖淀粉酶和α-淀粉酶上作为融合蛋白质与糖链结合蛋白的凝集功能结构域存在的酵母。