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1. 发明申请

WO2003004691A2 REGULATORS OF BIOFILM FORMATION AND USES THEREOF 审中-公开
标题翻译：生物膜形成的调节剂及其用途
公开(公告)号：WO2003004691A2
公开(公告)日：2003-01-16
申请号：PCT/US2002/023242
申请日：2002-07-05
申请人： THE GENERAL HOSPITAL CORPORATION , AUSUBEL, Frederic, M. , DRENKARD, Eliana
发明人： AUSUBEL, Frederic, M. , DRENKARD, Eliana
IPC分类号： C12Q
CPC分类号： C07K14/21 , A61K38/00
摘要： This invention relates to nucleic acid and amino acid sequences of genes regulating bacterial biofilm formation and to the use of these sequences as targets in the diagnosis, treatment, and prevention of bacterial infection and pathogenesis. In addition, the invention relates to screening methods for identifying modulators of bacterial biofilm formation and the development of antibacterial treatments.
摘要翻译：本发明涉及调节细菌生物膜形成的基因的核酸和氨基酸序列以及这些序列作为诊断，治疗和预防细菌感染和发病机制中的靶标的用途。此外，本发明涉及用于鉴定细菌生物膜形成调节剂和抗菌处理开发的筛选方法。

2. 发明申请

WO9929901A9 BROAD RANGE PCR AMPLIFICATION TECHNIQUES 审中-公开
标题翻译： BROAD RANGE PCR扩增技术
公开(公告)号：WO9929901A9
公开(公告)日：1999-09-16
申请号：PCT/US9825665
申请日：1998-12-03
申请人： GEN HOSPITAL CORP , AUSUBEL FREDERICK M , MINDRINOS MICHAEL , DRENKARD ELIANA
发明人： AUSUBEL FREDERICK M , MINDRINOS MICHAEL , DRENKARD ELIANA
IPC分类号： C12Q1/68 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/6858
摘要： Disclosed herein are methods and kits for determining whether a nucleic acid sequence includes a particular allele of a polymorphic sequence. One such method includes the steps of: (a) contacting a nucleic acid sequence, in the same or a separate reaction, with a first pair of PCR primers and a second pair of PCR primers under conditions that allow hybridization of the PCR primers to the nucleic acid sequence, the first pair of PCR primers hybridizing to opposite strands of the nucleic acid sequence and bordering the position of the polymorphic sequence, and the second pair of PCR primers hybridizing to opposite strands of the nucleic acid sequence and bordering the position of the polymorphic sequence, the PCR primers being characterized as follows: (i) one of the first pair of PCR primers (a) being complementary at its 3'-terminal nucleotide to a first allele of the polymorphic sequence (allele A), (b) being non-complementary at its 3'-terminal nucleotide to a second allele of the polymorphic sequence (allele B), and (c) being non-complementary to the nucleic acid sequence at a single non-complementary nucleotide in its 3'-terminal nucleotides 2-6; and (ii) one of the second pair of PCR primers (a) being complementary at its 3'-terminal nucleotide to the first allele of the polymorphic sequence (allele A), (b) being non-complementary at its 3'-terminal nucleotide to the second allele of the polymorphic sequence (allele B), and (c) being non-complementary to the nucleic acid sequence at one (and, preferably, two) or more nucleotides in its 3'-terminal nucleotides 2-6; (b) carrying out the amplification reactions; and (c) detecting an amplification product as an indication of the presence, in the nucleic acid sequence, of the first allele of the polymorphic sequence (allele A). In a preferred embodiment, this method is used to identify single nucleotide polymorphisms, for example, for genomic mapping purposes.
摘要翻译：本文公开了用于确定核酸序列是否包括多态性序列的特定等位基因的方法和试剂盒。一种这样的方法包括以下步骤：（a）在相同或单独的反应中将核酸序列与第一对PCR引物和第二对PCR引物在允许PCR引物与核酸序列，第一对PCR引物与核酸序列的相对链杂交并与多态性序列的位置相邻，第二对PCR引物与核酸序列的相对链杂交并与多态性序列，PCR引物的特征如下：（i）第一对PCR引物（a）之一在其3'末端核苷酸与多态性序列（等位基因A）的第一等位基因互补，（b）在其3'末端核苷酸处与多态性序列（等位基因B）的第二等位基因不互补，和（c）与其3'末端的单个非互补核苷酸与核酸序列不互补，末端核苷酸2-6; 和（ii）第二对PCR引物（a）之一在其3'末端核苷酸与多态性序列（等位基因A）的第一等位基因互补，（b）在其3'端不互补核苷酸至多态性序列（等位基因B）的第二等位基因，和（c）与其3'末端核苷酸2-6中的一个（优选2个）以上核苷酸与核酸序列不互补; （b）进行扩增反应; 和（c）检测扩增产物作为在核酸序列中存在多态性序列（等位基因A）的第一等位基因的指示。在优选的实施方案中，该方法用于鉴定单核苷酸多态性，例如用于基因组测绘目的。

3. 发明申请

WO2003038116A2 SALMONELLA VIRULENCE FACTORS AND USES THEREOF 审中-公开
标题翻译： SALMONELLA VIRULENCE因子及其用途
公开(公告)号：WO2003038116A2
公开(公告)日：2003-05-08
申请号：PCT/US2002/035039
申请日：2002-11-01
申请人： THE GENERAL HOSPITAL CORPORATION , AUSUBEL, Frederick, M. , ABALLAY, Alejandro , DRENKARD, Eliana , MCCORMICK, Beth, A.
发明人： AUSUBEL, Frederick, M. , ABALLAY, Alejandro , DRENKARD, Eliana , MCCORMICK, Beth, A.
IPC分类号： C12Q
CPC分类号： C07K14/255
摘要： The invention features nucleic acid molecules encoding microbial virulence factors (such as gmhA ) and methods of using such gene (and their proteins) as targets to identify anti-pathogenic agents. The invention also features a method of identifying a compound that inhibits the pathogenicity of an effector protein in a nematode, the method involving the steps of: (a) providing a nematode expressing an effector protein; (b) contacting the nematode with a test compound; and (c) determining whether the test compound inhibits the pathogenicity of the effector polypeptide in the nematode.
摘要翻译：本发明的特征在于编码微生物毒力因子（例如，gmhA）的核酸分子和使用该基因（及其蛋白质）作为靶标的方法来鉴定抗病原体。本发明还涉及鉴定抑制线虫中效应蛋白的致病性的化合物的方法，所述方法包括以下步骤：（a）提供表达效应蛋白的线虫; （b）使线虫与测试化合物接触; 和（c）确定测试化合物是否抑制线虫中效应多肽的致病性。

4. 发明申请

WO03004691A3 REGULATORS OF BIOFILM FORMATION AND USES THEREOF 审中-公开
标题翻译：生物膜形成的调节剂及其用途
公开(公告)号：WO03004691A3
公开(公告)日：2003-05-08
申请号：PCT/US0223242
申请日：2002-07-05
申请人： GEN HOSPITAL CORP , AUSUBEL FREDERIC M , DRENKARD ELIANA
发明人： AUSUBEL FREDERIC M , DRENKARD ELIANA
IPC分类号： A61K38/00 , C07H21/04 , C07K14/195 , C07K14/21 , C12N1/21 , C12N15/74 , C12Q1/18 , C12Q1/68 , A61K7/22 , A61K7/24 , A61K38/51 , C12N1/12 , C12N1/20 , C12N9/00 , C12N9/24 , C12N9/99
CPC分类号： C07K14/21 , A61K38/00
摘要： This invention relates to nucleic acid and amino acid sequences of genes regulating bacterial biofilm formation and to the use of these sequences as targets in the diagnosis, treatment, and prevention of bacterial infection and pathogenesis. In addition, the invention relates to screening methods for identifying modulators of bacterial biofilm formation and the development of antibacterial treatments.
摘要翻译：本发明涉及调节细菌生物膜形成的基因的核酸和氨基酸序列，并涉及这些序列作为诊断，治疗和预防细菌感染和发病机理中的靶标的用途。另外，本发明涉及用于鉴定细菌生物膜形成的调节剂和抗菌治疗的发展的筛选方法。

5. 发明申请

WO2003004689A2 REGULATORS OF BIOFILM FORMATION AND USES THEREOF 审中-公开
标题翻译：生物膜形成的调节剂及其用途
公开(公告)号：WO2003004689A2
公开(公告)日：2003-01-16
申请号：PCT/US2002/021431
申请日：2002-07-08
申请人： THE GENERAL HOSPITAL CORPORATION , AUSUBEL, Frederick, M. , DRENKARD, Eliana
发明人： AUSUBEL, Frederick, M. , DRENKARD, Eliana
IPC分类号： C12Q
CPC分类号： C07K14/21 , A61K38/00
摘要： This invention relates to nucleic acid and amino acid sequences of genes regulating bacterial biofilm formation and to the use of these sequences as targets in the diagnosis, treatment, and prevention of bacterial infection and pathogenesis. In addition, the invention relates to screening methods for identifying modulators of bacterial biofilm formation and the development of antibacterial treatments.
摘要翻译：本发明涉及调节细菌生物膜形成的基因的核酸和氨基酸序列，并涉及这些序列作为诊断，治疗和预防细菌感染和发病机理中的靶标的用途。此外，本发明涉及鉴定细菌生物膜形成的调节剂和开发抗菌治疗的筛选方法。

6. 发明申请

WO03038116A3 SALMONELLA VIRULENCE FACTORS AND USES THEREOF 审中-公开
标题翻译： SALMONELLA VIRULENCE因子及其用途
公开(公告)号：WO03038116A3
公开(公告)日：2003-10-16
申请号：PCT/US0235039
申请日：2002-11-01
申请人： GEN HOSPITAL CORP , AUSUBEL FREDERICK M , ABALLAY ALEJANDRO , DRENKARD ELIANA , MCCORMICK BETH A
发明人： AUSUBEL FREDERICK M , ABALLAY ALEJANDRO , DRENKARD ELIANA , MCCORMICK BETH A
IPC分类号： C07K14/255 , C07H21/04 , A61K39/02 , A61K39/108 , A61K39/112 , C07K1/00 , C12N1/20 , C12N15/00 , C12Q1/68 , G01N33/53
CPC分类号： C07K14/255
摘要： The invention features nucleic acid molecules encoding microbial virulence factors (such as gmhA) and methods of using such gene (and their proteins) as targets to identify anti-pathogenic agents. The invention also features a method of identifying a compound that inhibits the pathogenicity of an effector protein in a nematode, the method involving the steps of: (a) providing a nematode expressing an effector protein; (b) contacting the nematode with a test compound; and (c) determining whether the test compound inhibits the pathogenicity of the effector polypeptide in the nematode.
摘要翻译：本发明的特征在于编码微生物毒力因子（例如gmhA）的核酸分子和使用这种基因（及其蛋白质）作为靶标的方法来鉴定抗病原体。本发明还涉及鉴定抑制线虫中效应蛋白的致病性的化合物的方法，所述方法包括以下步骤：（a）提供表达效应蛋白的线虫; （b）使线虫与测试化合物接触; 和（c）确定测试化合物是否抑制线虫中效应多肽的致病性。

7. 发明申请

WO2003004689A3 REGULATORS OF BIOFILM FORMATION AND USES THEREOF 审中-公开
公开(公告)号：WO2003004689A3
公开(公告)日：2003-01-16
申请号：PCT/US2002/021431
申请日：2002-07-08
申请人： THE GENERAL HOSPITAL CORPORATION , AUSUBEL, Frederick, M. , DRENKARD, Eliana
发明人： AUSUBEL, Frederick, M. , DRENKARD, Eliana
IPC分类号： C12P21/06
摘要： This invention relates to nucleic acid and amino acid sequences of genes regulating bacterial biofilm formation and to the use of these sequences as targets in the diagnosis, treatment, and prevention of bacterial infection and pathogenesis. In addition, the invention relates to screening methods for identifying modulators of bacterial biofilm formation and the development of antibacterial treatments.

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